Comparative Study of Proteolytic Activities of Venom from Adult and Juvenile Species of Bothrops Alternatus

Autores
Garcia Denegri, María Emilia; Van de Velde, Andrea Carolina; Gomez, Gabriela Noemi; Nuñez, Sandra Elizabeth; Otto, Bárbara Vanesa; Leiva, Laura Cristina Ana; Teibler, Gladys Pamela
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Because of the technological advance in proteomic essays, ontogenic variations studies about snake venoms have become more relevant in the last years. The differences would be associated with differences in the snake venom protein composition and could have importance in the selection of the samples for production and control of quality of antivenoms. On the other hand, some authors suggested the use of venom from young snakes (yV) in order to purify some of the main components present in major proportions than in venom from adult snakes (aV). In this study, we compared venoms from Bothrops alternatus (yarará grande) from both stages of ontogeny, focusing on the study of the protein bands by SDS-PAGE and also on the study of proteolytic activities (metalloproteinases and serineproteases enzymes) exhibited by these venoms as the main responsible for the physiopathological action exhibited by this animal. Proteolytic activity was assessing by azocasein method, coagulant activity by citrated plasma and then, amidolytic actitivty was evaluated on BApNA. The results showed differences not only in the protein profile, but also in the enzymatic activities from young and adult snake venoms. Young snake venom coagulated the citrated plasma in the half time (6.3 s) the adult snake venom did. The relation yV/aV of proteolytic activity assessed on azocasein was 2 and 23 in the case of amydolitc action. These results highlight the differences in metalloproteinases concentration (responsible of caseinolytic activity) and especially in the serineproteinases composition (tested on BApNA), which would express in low quantities in adult snake venoms. These evidences warn about the necessity to assay the level of protection that the standart antivenom produces against a potential snakebite by young species and, at the same time, look for the development of antivenoms with pools including young and adult organisms.
Fil: Garcia Denegri, María Emilia. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Van de Velde, Andrea Carolina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Gomez, Gabriela Noemi. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Nuñez, Sandra Elizabeth. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Otto, Bárbara Vanesa. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Leiva, Laura Cristina Ana. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Teibler, Gladys Pamela. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina
XX Annual Meeting of the Argentinean Biology Society and VII Meeting of the Uruguayan Society of Biosciences
Buenos Aires
Argentina
Sociedad Argentina de Biología
Sociedad Uruguaya de Biociencias
Materia
BOTHROPS ALTERNATUS
BAPNA
PROTEASES
CASEIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/181344
Acceder
id CONICETDig_f82b977f35d4590c846f2bf1c8c2355d
oai_identifier_str oai:ri.conicet.gov.ar:11336/181344
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparative Study of Proteolytic Activities of Venom from Adult and Juvenile Species of Bothrops AlternatusGarcia Denegri, María EmiliaVan de Velde, Andrea CarolinaGomez, Gabriela NoemiNuñez, Sandra ElizabethOtto, Bárbara VanesaLeiva, Laura Cristina AnaTeibler, Gladys PamelaBOTHROPS ALTERNATUSBAPNAPROTEASESCASEINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Because of the technological advance in proteomic essays, ontogenic variations studies about snake venoms have become more relevant in the last years. The differences would be associated with differences in the snake venom protein composition and could have importance in the selection of the samples for production and control of quality of antivenoms. On the other hand, some authors suggested the use of venom from young snakes (yV) in order to purify some of the main components present in major proportions than in venom from adult snakes (aV). In this study, we compared venoms from Bothrops alternatus (yarará grande) from both stages of ontogeny, focusing on the study of the protein bands by SDS-PAGE and also on the study of proteolytic activities (metalloproteinases and serineproteases enzymes) exhibited by these venoms as the main responsible for the physiopathological action exhibited by this animal. Proteolytic activity was assessing by azocasein method, coagulant activity by citrated plasma and then, amidolytic actitivty was evaluated on BApNA. The results showed differences not only in the protein profile, but also in the enzymatic activities from young and adult snake venoms. Young snake venom coagulated the citrated plasma in the half time (6.3 s) the adult snake venom did. The relation yV/aV of proteolytic activity assessed on azocasein was 2 and 23 in the case of amydolitc action. These results highlight the differences in metalloproteinases concentration (responsible of caseinolytic activity) and especially in the serineproteinases composition (tested on BApNA), which would express in low quantities in adult snake venoms. These evidences warn about the necessity to assay the level of protection that the standart antivenom produces against a potential snakebite by young species and, at the same time, look for the development of antivenoms with pools including young and adult organisms.Fil: Garcia Denegri, María Emilia. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Van de Velde, Andrea Carolina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: Gomez, Gabriela Noemi. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: Nuñez, Sandra Elizabeth. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Otto, Bárbara Vanesa. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Leiva, Laura Cristina Ana. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Teibler, Gladys Pamela. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; ArgentinaXX Annual Meeting of the Argentinean Biology Society and VII Meeting of the Uruguayan Society of BiosciencesBuenos AiresArgentinaSociedad Argentina de BiologíaSociedad Uruguaya de BiocienciasTech Science Press2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/181344Comparative Study of Proteolytic Activities of Venom from Adult and Juvenile Species of Bothrops Alternatus; XX Annual Meeting of the Argentinean Biology Society and VII Meeting of the Uruguayan Society of Biosciences; Buenos Aires; Argentina; 2018; 1-20327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v43nSuppl.3/33866Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:04:09Zoai:ri.conicet.gov.ar:11336/181344instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:04:09.262CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparative Study of Proteolytic Activities of Venom from Adult and Juvenile Species of Bothrops Alternatus
title Comparative Study of Proteolytic Activities of Venom from Adult and Juvenile Species of Bothrops Alternatus
spellingShingle Comparative Study of Proteolytic Activities of Venom from Adult and Juvenile Species of Bothrops Alternatus
Garcia Denegri, María Emilia
BOTHROPS ALTERNATUS
BAPNA
PROTEASES
CASEIN
title_short Comparative Study of Proteolytic Activities of Venom from Adult and Juvenile Species of Bothrops Alternatus
title_full Comparative Study of Proteolytic Activities of Venom from Adult and Juvenile Species of Bothrops Alternatus
title_fullStr Comparative Study of Proteolytic Activities of Venom from Adult and Juvenile Species of Bothrops Alternatus
title_full_unstemmed Comparative Study of Proteolytic Activities of Venom from Adult and Juvenile Species of Bothrops Alternatus
title_sort Comparative Study of Proteolytic Activities of Venom from Adult and Juvenile Species of Bothrops Alternatus
dc.creator.none.fl_str_mv Garcia Denegri, María Emilia
Van de Velde, Andrea Carolina
Gomez, Gabriela Noemi
Nuñez, Sandra Elizabeth
Otto, Bárbara Vanesa
Leiva, Laura Cristina Ana
Teibler, Gladys Pamela
author Garcia Denegri, María Emilia
author_facet Garcia Denegri, María Emilia
Van de Velde, Andrea Carolina
Gomez, Gabriela Noemi
Nuñez, Sandra Elizabeth
Otto, Bárbara Vanesa
Leiva, Laura Cristina Ana
Teibler, Gladys Pamela
author_role author
author2 Van de Velde, Andrea Carolina
Gomez, Gabriela Noemi
Nuñez, Sandra Elizabeth
Otto, Bárbara Vanesa
Leiva, Laura Cristina Ana
Teibler, Gladys Pamela
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BOTHROPS ALTERNATUS
BAPNA
PROTEASES
CASEIN
topic BOTHROPS ALTERNATUS
BAPNA
PROTEASES
CASEIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Because of the technological advance in proteomic essays, ontogenic variations studies about snake venoms have become more relevant in the last years. The differences would be associated with differences in the snake venom protein composition and could have importance in the selection of the samples for production and control of quality of antivenoms. On the other hand, some authors suggested the use of venom from young snakes (yV) in order to purify some of the main components present in major proportions than in venom from adult snakes (aV). In this study, we compared venoms from Bothrops alternatus (yarará grande) from both stages of ontogeny, focusing on the study of the protein bands by SDS-PAGE and also on the study of proteolytic activities (metalloproteinases and serineproteases enzymes) exhibited by these venoms as the main responsible for the physiopathological action exhibited by this animal. Proteolytic activity was assessing by azocasein method, coagulant activity by citrated plasma and then, amidolytic actitivty was evaluated on BApNA. The results showed differences not only in the protein profile, but also in the enzymatic activities from young and adult snake venoms. Young snake venom coagulated the citrated plasma in the half time (6.3 s) the adult snake venom did. The relation yV/aV of proteolytic activity assessed on azocasein was 2 and 23 in the case of amydolitc action. These results highlight the differences in metalloproteinases concentration (responsible of caseinolytic activity) and especially in the serineproteinases composition (tested on BApNA), which would express in low quantities in adult snake venoms. These evidences warn about the necessity to assay the level of protection that the standart antivenom produces against a potential snakebite by young species and, at the same time, look for the development of antivenoms with pools including young and adult organisms.
Fil: Garcia Denegri, María Emilia. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Van de Velde, Andrea Carolina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Gomez, Gabriela Noemi. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Nuñez, Sandra Elizabeth. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Otto, Bárbara Vanesa. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Leiva, Laura Cristina Ana. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Teibler, Gladys Pamela. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina
XX Annual Meeting of the Argentinean Biology Society and VII Meeting of the Uruguayan Society of Biosciences
Buenos Aires
Argentina
Sociedad Argentina de Biología
Sociedad Uruguaya de Biociencias
description Because of the technological advance in proteomic essays, ontogenic variations studies about snake venoms have become more relevant in the last years. The differences would be associated with differences in the snake venom protein composition and could have importance in the selection of the samples for production and control of quality of antivenoms. On the other hand, some authors suggested the use of venom from young snakes (yV) in order to purify some of the main components present in major proportions than in venom from adult snakes (aV). In this study, we compared venoms from Bothrops alternatus (yarará grande) from both stages of ontogeny, focusing on the study of the protein bands by SDS-PAGE and also on the study of proteolytic activities (metalloproteinases and serineproteases enzymes) exhibited by these venoms as the main responsible for the physiopathological action exhibited by this animal. Proteolytic activity was assessing by azocasein method, coagulant activity by citrated plasma and then, amidolytic actitivty was evaluated on BApNA. The results showed differences not only in the protein profile, but also in the enzymatic activities from young and adult snake venoms. Young snake venom coagulated the citrated plasma in the half time (6.3 s) the adult snake venom did. The relation yV/aV of proteolytic activity assessed on azocasein was 2 and 23 in the case of amydolitc action. These results highlight the differences in metalloproteinases concentration (responsible of caseinolytic activity) and especially in the serineproteinases composition (tested on BApNA), which would express in low quantities in adult snake venoms. These evidences warn about the necessity to assay the level of protection that the standart antivenom produces against a potential snakebite by young species and, at the same time, look for the development of antivenoms with pools including young and adult organisms.
publishDate 2018
dc.date.none.fl_str_mv 2018
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info:eu-repo/semantics/conferenceObject
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http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/181344
Comparative Study of Proteolytic Activities of Venom from Adult and Juvenile Species of Bothrops Alternatus; XX Annual Meeting of the Argentinean Biology Society and VII Meeting of the Uruguayan Society of Biosciences; Buenos Aires; Argentina; 2018; 1-2
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/181344
identifier_str_mv Comparative Study of Proteolytic Activities of Venom from Adult and Juvenile Species of Bothrops Alternatus; XX Annual Meeting of the Argentinean Biology Society and VII Meeting of the Uruguayan Society of Biosciences; Buenos Aires; Argentina; 2018; 1-2
0327-9545
1667-5746
CONICET Digital
CONICET
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publisher.none.fl_str_mv Tech Science Press
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