Characterization of the killer toxin KTCf20 from wickerhamomyces anomalus, a potential biocontrol agent against wine spoilage yeasts

Autores
Fernandez de Ullivarri, Miguel; Mendoza, Lucia Margarita; Raya, Raul Ricardo
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Wickerhamomyces anomalus Cf20 secretes the killer toxin KTCf20 that inhibits several wine spoilage yeasts of the species Pichia guilliermondii, P. membranifaciens, Brettanomyces bruxellensis and Dekkera anomala. KTCf20 binds cell wall extracts from the sensitive target P. guilliermondii Cd6; however, this capacity was lost when cell wall extracts were pre-treated with fungal β-glucanase. Pustulan and laminarin inhibited killer activity, suggesting that β-1,3 and β-1,6-glucans may be the putative binding sites for KTCf20 on the cell wall of sensitive cells. The toxin was produced and showed to be stable and highly active at physicochemical conditions suitable for winemaking process. In addition, the strain Cf20 is compatible with Saccharomyces cerevisiae in co-culture conditions being potential its application in a mixed starter culture. These data suggest that W. anomalus Cf20 and/or KTCf20 are promising biocontrol agents against spoilage yeasts during wine-making process.
Fil: Fernandez de Ullivarri, Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Mendoza, Lucia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
BIOCONTROL
KILLER TOXIN
SPOILAGE YEASTS
WICKERHAMOMYCES ANOMALUS
WINEMAKING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/81451
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/81451
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of the killer toxin KTCf20 from wickerhamomyces anomalus, a potential biocontrol agent against wine spoilage yeastsFernandez de Ullivarri, MiguelMendoza, Lucia MargaritaRaya, Raul RicardoBIOCONTROLKILLER TOXINSPOILAGE YEASTSWICKERHAMOMYCES ANOMALUSWINEMAKINGhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Wickerhamomyces anomalus Cf20 secretes the killer toxin KTCf20 that inhibits several wine spoilage yeasts of the species Pichia guilliermondii, P. membranifaciens, Brettanomyces bruxellensis and Dekkera anomala. KTCf20 binds cell wall extracts from the sensitive target P. guilliermondii Cd6; however, this capacity was lost when cell wall extracts were pre-treated with fungal β-glucanase. Pustulan and laminarin inhibited killer activity, suggesting that β-1,3 and β-1,6-glucans may be the putative binding sites for KTCf20 on the cell wall of sensitive cells. The toxin was produced and showed to be stable and highly active at physicochemical conditions suitable for winemaking process. In addition, the strain Cf20 is compatible with Saccharomyces cerevisiae in co-culture conditions being potential its application in a mixed starter culture. These data suggest that W. anomalus Cf20 and/or KTCf20 are promising biocontrol agents against spoilage yeasts during wine-making process.Fil: Fernandez de Ullivarri, Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Mendoza, Lucia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaElsevier2018-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/81451Fernandez de Ullivarri, Miguel; Mendoza, Lucia Margarita; Raya, Raul Ricardo; Characterization of the killer toxin KTCf20 from wickerhamomyces anomalus, a potential biocontrol agent against wine spoilage yeasts; Elsevier; Biological Control; 121; 6-2018; 223-2281049-9644CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1049964418301658?via%3Dihubinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.biocontrol.2018.03.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:04Zoai:ri.conicet.gov.ar:11336/81451instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:05.217CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of the killer toxin KTCf20 from wickerhamomyces anomalus, a potential biocontrol agent against wine spoilage yeasts
title Characterization of the killer toxin KTCf20 from wickerhamomyces anomalus, a potential biocontrol agent against wine spoilage yeasts
spellingShingle Characterization of the killer toxin KTCf20 from wickerhamomyces anomalus, a potential biocontrol agent against wine spoilage yeasts
Fernandez de Ullivarri, Miguel
BIOCONTROL
KILLER TOXIN
SPOILAGE YEASTS
WICKERHAMOMYCES ANOMALUS
WINEMAKING
title_short Characterization of the killer toxin KTCf20 from wickerhamomyces anomalus, a potential biocontrol agent against wine spoilage yeasts
title_full Characterization of the killer toxin KTCf20 from wickerhamomyces anomalus, a potential biocontrol agent against wine spoilage yeasts
title_fullStr Characterization of the killer toxin KTCf20 from wickerhamomyces anomalus, a potential biocontrol agent against wine spoilage yeasts
title_full_unstemmed Characterization of the killer toxin KTCf20 from wickerhamomyces anomalus, a potential biocontrol agent against wine spoilage yeasts
title_sort Characterization of the killer toxin KTCf20 from wickerhamomyces anomalus, a potential biocontrol agent against wine spoilage yeasts
dc.creator.none.fl_str_mv Fernandez de Ullivarri, Miguel
Mendoza, Lucia Margarita
Raya, Raul Ricardo
author Fernandez de Ullivarri, Miguel
author_facet Fernandez de Ullivarri, Miguel
Mendoza, Lucia Margarita
Raya, Raul Ricardo
author_role author
author2 Mendoza, Lucia Margarita
Raya, Raul Ricardo
author2_role author
author
dc.subject.none.fl_str_mv BIOCONTROL
KILLER TOXIN
SPOILAGE YEASTS
WICKERHAMOMYCES ANOMALUS
WINEMAKING
topic BIOCONTROL
KILLER TOXIN
SPOILAGE YEASTS
WICKERHAMOMYCES ANOMALUS
WINEMAKING
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Wickerhamomyces anomalus Cf20 secretes the killer toxin KTCf20 that inhibits several wine spoilage yeasts of the species Pichia guilliermondii, P. membranifaciens, Brettanomyces bruxellensis and Dekkera anomala. KTCf20 binds cell wall extracts from the sensitive target P. guilliermondii Cd6; however, this capacity was lost when cell wall extracts were pre-treated with fungal β-glucanase. Pustulan and laminarin inhibited killer activity, suggesting that β-1,3 and β-1,6-glucans may be the putative binding sites for KTCf20 on the cell wall of sensitive cells. The toxin was produced and showed to be stable and highly active at physicochemical conditions suitable for winemaking process. In addition, the strain Cf20 is compatible with Saccharomyces cerevisiae in co-culture conditions being potential its application in a mixed starter culture. These data suggest that W. anomalus Cf20 and/or KTCf20 are promising biocontrol agents against spoilage yeasts during wine-making process.
Fil: Fernandez de Ullivarri, Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Mendoza, Lucia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description Wickerhamomyces anomalus Cf20 secretes the killer toxin KTCf20 that inhibits several wine spoilage yeasts of the species Pichia guilliermondii, P. membranifaciens, Brettanomyces bruxellensis and Dekkera anomala. KTCf20 binds cell wall extracts from the sensitive target P. guilliermondii Cd6; however, this capacity was lost when cell wall extracts were pre-treated with fungal β-glucanase. Pustulan and laminarin inhibited killer activity, suggesting that β-1,3 and β-1,6-glucans may be the putative binding sites for KTCf20 on the cell wall of sensitive cells. The toxin was produced and showed to be stable and highly active at physicochemical conditions suitable for winemaking process. In addition, the strain Cf20 is compatible with Saccharomyces cerevisiae in co-culture conditions being potential its application in a mixed starter culture. These data suggest that W. anomalus Cf20 and/or KTCf20 are promising biocontrol agents against spoilage yeasts during wine-making process.
publishDate 2018
dc.date.none.fl_str_mv 2018-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/81451
Fernandez de Ullivarri, Miguel; Mendoza, Lucia Margarita; Raya, Raul Ricardo; Characterization of the killer toxin KTCf20 from wickerhamomyces anomalus, a potential biocontrol agent against wine spoilage yeasts; Elsevier; Biological Control; 121; 6-2018; 223-228
1049-9644
CONICET Digital
CONICET
url http://hdl.handle.net/11336/81451
identifier_str_mv Fernandez de Ullivarri, Miguel; Mendoza, Lucia Margarita; Raya, Raul Ricardo; Characterization of the killer toxin KTCf20 from wickerhamomyces anomalus, a potential biocontrol agent against wine spoilage yeasts; Elsevier; Biological Control; 121; 6-2018; 223-228
1049-9644
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1049964418301658?via%3Dihub
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biocontrol.2018.03.008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270068204896256
score 13.13397

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