Non-target-site glyphosate resistance in Echinochloa colona from Western Australia

Autores
Goh, Sou Sheng; Yu, Qin; Han, Heping; Vila Aiub, Martin Miguel; Busi, Roberto; Powles, Stephen B.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An Echinochloa colona population from Western Australia has evolved resistance to glyphosate. This current study investigates the physiological, molecular and biochemical basis of glyphosate resistance in this population. To minimise genetic differences the susceptible (S) and resistance (R) phenotypes were isolated from within this resistant population. The S phenotype was found to accumulate significantly more shikimate in the leaf tissue than the R phenotype following glyphosate treatment. Target-site EPSPS gene sequencing revealed no resistance mutations and EPSPS gene expression was similar between the S and R phenotypes. Thus, glyphosate resistance in this population is unlikely target-site based. Similarly, there were no significant differences between the S and R phenotypes in glyphosate leaf uptake and translocation at the whole plant level. It is also unlikely that the resistance is associated with glyphosate metabolism as no major glyphosate metabolites were detected in leaf tissue of the S and R phenotypes. Despite much effort the exact glyphosate resistance mechanisms in this R population remain unclear, and novel resistance mechanisms are to be determined.
Fil: Goh, Sou Sheng. Malaysian Rubber Board; Malasia. University of Western Australia; Australia
Fil: Yu, Qin. University of Western Australia; Australia
Fil: Han, Heping. University of Western Australia; Australia
Fil: Vila Aiub, Martin Miguel. University of Western Australia; Australia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Busi, Roberto. University of Western Australia; Australia
Fil: Powles, Stephen B.. University of Western Australia; Australia
Materia
ECHINOCHLOA COLONA
EPSPS
GLYPHOSATE
NON-TARGET-SITE RESISTANCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/93156
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Non-target-site glyphosate resistance in Echinochloa colona from Western AustraliaGoh, Sou ShengYu, QinHan, HepingVila Aiub, Martin MiguelBusi, RobertoPowles, Stephen B.ECHINOCHLOA COLONAEPSPSGLYPHOSATENON-TARGET-SITE RESISTANCEhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4An Echinochloa colona population from Western Australia has evolved resistance to glyphosate. This current study investigates the physiological, molecular and biochemical basis of glyphosate resistance in this population. To minimise genetic differences the susceptible (S) and resistance (R) phenotypes were isolated from within this resistant population. The S phenotype was found to accumulate significantly more shikimate in the leaf tissue than the R phenotype following glyphosate treatment. Target-site EPSPS gene sequencing revealed no resistance mutations and EPSPS gene expression was similar between the S and R phenotypes. Thus, glyphosate resistance in this population is unlikely target-site based. Similarly, there were no significant differences between the S and R phenotypes in glyphosate leaf uptake and translocation at the whole plant level. It is also unlikely that the resistance is associated with glyphosate metabolism as no major glyphosate metabolites were detected in leaf tissue of the S and R phenotypes. Despite much effort the exact glyphosate resistance mechanisms in this R population remain unclear, and novel resistance mechanisms are to be determined.Fil: Goh, Sou Sheng. Malaysian Rubber Board; Malasia. University of Western Australia; AustraliaFil: Yu, Qin. University of Western Australia; AustraliaFil: Han, Heping. University of Western Australia; AustraliaFil: Vila Aiub, Martin Miguel. University of Western Australia; Australia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Busi, Roberto. University of Western Australia; AustraliaFil: Powles, Stephen B.. University of Western Australia; AustraliaElsevier2018-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93156Goh, Sou Sheng; Yu, Qin; Han, Heping; Vila Aiub, Martin Miguel; Busi, Roberto; et al.; Non-target-site glyphosate resistance in Echinochloa colona from Western Australia; Elsevier; Crop Protection; 112; 10-2018; 257-2630261-2194CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.cropro.2018.06.013info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0261219418301686info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:02Zoai:ri.conicet.gov.ar:11336/93156instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:03.007CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Non-target-site glyphosate resistance in Echinochloa colona from Western Australia
title Non-target-site glyphosate resistance in Echinochloa colona from Western Australia
spellingShingle Non-target-site glyphosate resistance in Echinochloa colona from Western Australia
Goh, Sou Sheng
ECHINOCHLOA COLONA
EPSPS
GLYPHOSATE
NON-TARGET-SITE RESISTANCE
title_short Non-target-site glyphosate resistance in Echinochloa colona from Western Australia
title_full Non-target-site glyphosate resistance in Echinochloa colona from Western Australia
title_fullStr Non-target-site glyphosate resistance in Echinochloa colona from Western Australia
title_full_unstemmed Non-target-site glyphosate resistance in Echinochloa colona from Western Australia
title_sort Non-target-site glyphosate resistance in Echinochloa colona from Western Australia
dc.creator.none.fl_str_mv Goh, Sou Sheng
Yu, Qin
Han, Heping
Vila Aiub, Martin Miguel
Busi, Roberto
Powles, Stephen B.
author Goh, Sou Sheng
author_facet Goh, Sou Sheng
Yu, Qin
Han, Heping
Vila Aiub, Martin Miguel
Busi, Roberto
Powles, Stephen B.
author_role author
author2 Yu, Qin
Han, Heping
Vila Aiub, Martin Miguel
Busi, Roberto
Powles, Stephen B.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ECHINOCHLOA COLONA
EPSPS
GLYPHOSATE
NON-TARGET-SITE RESISTANCE
topic ECHINOCHLOA COLONA
EPSPS
GLYPHOSATE
NON-TARGET-SITE RESISTANCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv An Echinochloa colona population from Western Australia has evolved resistance to glyphosate. This current study investigates the physiological, molecular and biochemical basis of glyphosate resistance in this population. To minimise genetic differences the susceptible (S) and resistance (R) phenotypes were isolated from within this resistant population. The S phenotype was found to accumulate significantly more shikimate in the leaf tissue than the R phenotype following glyphosate treatment. Target-site EPSPS gene sequencing revealed no resistance mutations and EPSPS gene expression was similar between the S and R phenotypes. Thus, glyphosate resistance in this population is unlikely target-site based. Similarly, there were no significant differences between the S and R phenotypes in glyphosate leaf uptake and translocation at the whole plant level. It is also unlikely that the resistance is associated with glyphosate metabolism as no major glyphosate metabolites were detected in leaf tissue of the S and R phenotypes. Despite much effort the exact glyphosate resistance mechanisms in this R population remain unclear, and novel resistance mechanisms are to be determined.
Fil: Goh, Sou Sheng. Malaysian Rubber Board; Malasia. University of Western Australia; Australia
Fil: Yu, Qin. University of Western Australia; Australia
Fil: Han, Heping. University of Western Australia; Australia
Fil: Vila Aiub, Martin Miguel. University of Western Australia; Australia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Busi, Roberto. University of Western Australia; Australia
Fil: Powles, Stephen B.. University of Western Australia; Australia
description An Echinochloa colona population from Western Australia has evolved resistance to glyphosate. This current study investigates the physiological, molecular and biochemical basis of glyphosate resistance in this population. To minimise genetic differences the susceptible (S) and resistance (R) phenotypes were isolated from within this resistant population. The S phenotype was found to accumulate significantly more shikimate in the leaf tissue than the R phenotype following glyphosate treatment. Target-site EPSPS gene sequencing revealed no resistance mutations and EPSPS gene expression was similar between the S and R phenotypes. Thus, glyphosate resistance in this population is unlikely target-site based. Similarly, there were no significant differences between the S and R phenotypes in glyphosate leaf uptake and translocation at the whole plant level. It is also unlikely that the resistance is associated with glyphosate metabolism as no major glyphosate metabolites were detected in leaf tissue of the S and R phenotypes. Despite much effort the exact glyphosate resistance mechanisms in this R population remain unclear, and novel resistance mechanisms are to be determined.
publishDate 2018
dc.date.none.fl_str_mv 2018-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/93156
Goh, Sou Sheng; Yu, Qin; Han, Heping; Vila Aiub, Martin Miguel; Busi, Roberto; et al.; Non-target-site glyphosate resistance in Echinochloa colona from Western Australia; Elsevier; Crop Protection; 112; 10-2018; 257-263
0261-2194
CONICET Digital
CONICET
url http://hdl.handle.net/11336/93156
identifier_str_mv Goh, Sou Sheng; Yu, Qin; Han, Heping; Vila Aiub, Martin Miguel; Busi, Roberto; et al.; Non-target-site glyphosate resistance in Echinochloa colona from Western Australia; Elsevier; Crop Protection; 112; 10-2018; 257-263
0261-2194
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cropro.2018.06.013
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0261219418301686
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.885934

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