Mechanism of resistance to glyphosate in Lolium perenne from Argentina

Autores
Yanniccari, Marcos Ezequiel; Gómez Lobato, María Eugenia; Istilart, Carolina María; Natalucci, Claudia Luisa; Gimenez, Daniel Oscar; Castro, Ana Maria
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
n Argentina, glyphosate resistance was reported in a Lolium perenne population after 12 years of successful herbicide use. The aim of the current paper was to put in evidence for the mechanism of glyphosate resistance of this weed. Susceptible leaves treated with different doses of glyphosate and incubated in vitro showed an accumulation of shikimic acid of around three to five times the basal level, while no changes were detected in leaves of glyphosate-resistant plants. The resistance mechanism prevents shikimate accumulation in leaves, even under such tissue-isolation conditions. The activity of the glyphosate target enzyme (EPSPS: 5-enolpyruvylshikimate-3-phosphate synthase) was quantified at different herbicide concentrations. EPSPS from resistant plants showed no difference in glyphosate-sensitivity compared to EPSPS from susceptible plants, and, accordingly, no amino acid substitution causing mutations associated with resistance were found. While the glyphosate target enzymes were equally sensitive, the basal EPSPS activity in glyphosate resistant plants was approximately 3-fold higher than the EPSPS activity in susceptible plants. This increased EPSPS activity in glyphosate resistant plants was associated with a 15-fold higher expression of EPSPS compared with susceptible plants. Therefore, the over-expression of EPSPS appears to be the main mechanism responsible for resistance to glyphosate. This mechanism has a constitutive character and has important effects on plant fitness, as recently reported.
Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Barrow; Argentina
Fil: Gómez Lobato, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Istilart, Carolina María. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Barrow; Argentina
Fil: Natalucci, Claudia Luisa. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Gimenez, Daniel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Castro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina. Universidad Nacional de la Plata. Facultad de Agronomia. Departamento de Sanidad Vegetal; Argentina
Materia
EPSPS ACTIVITY
EPSPS OVEREXPRESSION
GLYPHOSATE RESISTANCE
PERENNIAL RYEGRASS
SHIKIMATE ASSAY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/66847
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/66847
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mechanism of resistance to glyphosate in Lolium perenne from ArgentinaYanniccari, Marcos EzequielGómez Lobato, María EugeniaIstilart, Carolina MaríaNatalucci, Claudia LuisaGimenez, Daniel OscarCastro, Ana MariaEPSPS ACTIVITYEPSPS OVEREXPRESSIONGLYPHOSATE RESISTANCEPERENNIAL RYEGRASSSHIKIMATE ASSAYhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4n Argentina, glyphosate resistance was reported in a Lolium perenne population after 12 years of successful herbicide use. The aim of the current paper was to put in evidence for the mechanism of glyphosate resistance of this weed. Susceptible leaves treated with different doses of glyphosate and incubated in vitro showed an accumulation of shikimic acid of around three to five times the basal level, while no changes were detected in leaves of glyphosate-resistant plants. The resistance mechanism prevents shikimate accumulation in leaves, even under such tissue-isolation conditions. The activity of the glyphosate target enzyme (EPSPS: 5-enolpyruvylshikimate-3-phosphate synthase) was quantified at different herbicide concentrations. EPSPS from resistant plants showed no difference in glyphosate-sensitivity compared to EPSPS from susceptible plants, and, accordingly, no amino acid substitution causing mutations associated with resistance were found. While the glyphosate target enzymes were equally sensitive, the basal EPSPS activity in glyphosate resistant plants was approximately 3-fold higher than the EPSPS activity in susceptible plants. This increased EPSPS activity in glyphosate resistant plants was associated with a 15-fold higher expression of EPSPS compared with susceptible plants. Therefore, the over-expression of EPSPS appears to be the main mechanism responsible for resistance to glyphosate. This mechanism has a constitutive character and has important effects on plant fitness, as recently reported.Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Barrow; ArgentinaFil: Gómez Lobato, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Istilart, Carolina María. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Barrow; ArgentinaFil: Natalucci, Claudia Luisa. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Gimenez, Daniel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Castro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina. Universidad Nacional de la Plata. Facultad de Agronomia. Departamento de Sanidad Vegetal; ArgentinaFrontiers Media S. A2017-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66847Yanniccari, Marcos Ezequiel; Gómez Lobato, María Eugenia; Istilart, Carolina María; Natalucci, Claudia Luisa; Gimenez, Daniel Oscar; et al.; Mechanism of resistance to glyphosate in Lolium perenne from Argentina; Frontiers Media S. A; Frontiers in Ecology and Evolution; 5; OCT; 10-2017; 1-82296-701XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fevo.2017.00123info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fevo.2017.00123info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:03:47Zoai:ri.conicet.gov.ar:11336/66847instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:03:47.921CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mechanism of resistance to glyphosate in Lolium perenne from Argentina
title Mechanism of resistance to glyphosate in Lolium perenne from Argentina
spellingShingle Mechanism of resistance to glyphosate in Lolium perenne from Argentina
Yanniccari, Marcos Ezequiel
EPSPS ACTIVITY
EPSPS OVEREXPRESSION
GLYPHOSATE RESISTANCE
PERENNIAL RYEGRASS
SHIKIMATE ASSAY
title_short Mechanism of resistance to glyphosate in Lolium perenne from Argentina
title_full Mechanism of resistance to glyphosate in Lolium perenne from Argentina
title_fullStr Mechanism of resistance to glyphosate in Lolium perenne from Argentina
title_full_unstemmed Mechanism of resistance to glyphosate in Lolium perenne from Argentina
title_sort Mechanism of resistance to glyphosate in Lolium perenne from Argentina
dc.creator.none.fl_str_mv Yanniccari, Marcos Ezequiel
Gómez Lobato, María Eugenia
Istilart, Carolina María
Natalucci, Claudia Luisa
Gimenez, Daniel Oscar
Castro, Ana Maria
author Yanniccari, Marcos Ezequiel
author_facet Yanniccari, Marcos Ezequiel
Gómez Lobato, María Eugenia
Istilart, Carolina María
Natalucci, Claudia Luisa
Gimenez, Daniel Oscar
Castro, Ana Maria
author_role author
author2 Gómez Lobato, María Eugenia
Istilart, Carolina María
Natalucci, Claudia Luisa
Gimenez, Daniel Oscar
Castro, Ana Maria
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv EPSPS ACTIVITY
EPSPS OVEREXPRESSION
GLYPHOSATE RESISTANCE
PERENNIAL RYEGRASS
SHIKIMATE ASSAY
topic EPSPS ACTIVITY
EPSPS OVEREXPRESSION
GLYPHOSATE RESISTANCE
PERENNIAL RYEGRASS
SHIKIMATE ASSAY
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv n Argentina, glyphosate resistance was reported in a Lolium perenne population after 12 years of successful herbicide use. The aim of the current paper was to put in evidence for the mechanism of glyphosate resistance of this weed. Susceptible leaves treated with different doses of glyphosate and incubated in vitro showed an accumulation of shikimic acid of around three to five times the basal level, while no changes were detected in leaves of glyphosate-resistant plants. The resistance mechanism prevents shikimate accumulation in leaves, even under such tissue-isolation conditions. The activity of the glyphosate target enzyme (EPSPS: 5-enolpyruvylshikimate-3-phosphate synthase) was quantified at different herbicide concentrations. EPSPS from resistant plants showed no difference in glyphosate-sensitivity compared to EPSPS from susceptible plants, and, accordingly, no amino acid substitution causing mutations associated with resistance were found. While the glyphosate target enzymes were equally sensitive, the basal EPSPS activity in glyphosate resistant plants was approximately 3-fold higher than the EPSPS activity in susceptible plants. This increased EPSPS activity in glyphosate resistant plants was associated with a 15-fold higher expression of EPSPS compared with susceptible plants. Therefore, the over-expression of EPSPS appears to be the main mechanism responsible for resistance to glyphosate. This mechanism has a constitutive character and has important effects on plant fitness, as recently reported.
Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Barrow; Argentina
Fil: Gómez Lobato, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Istilart, Carolina María. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Barrow; Argentina
Fil: Natalucci, Claudia Luisa. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Gimenez, Daniel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Castro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina. Universidad Nacional de la Plata. Facultad de Agronomia. Departamento de Sanidad Vegetal; Argentina
description n Argentina, glyphosate resistance was reported in a Lolium perenne population after 12 years of successful herbicide use. The aim of the current paper was to put in evidence for the mechanism of glyphosate resistance of this weed. Susceptible leaves treated with different doses of glyphosate and incubated in vitro showed an accumulation of shikimic acid of around three to five times the basal level, while no changes were detected in leaves of glyphosate-resistant plants. The resistance mechanism prevents shikimate accumulation in leaves, even under such tissue-isolation conditions. The activity of the glyphosate target enzyme (EPSPS: 5-enolpyruvylshikimate-3-phosphate synthase) was quantified at different herbicide concentrations. EPSPS from resistant plants showed no difference in glyphosate-sensitivity compared to EPSPS from susceptible plants, and, accordingly, no amino acid substitution causing mutations associated with resistance were found. While the glyphosate target enzymes were equally sensitive, the basal EPSPS activity in glyphosate resistant plants was approximately 3-fold higher than the EPSPS activity in susceptible plants. This increased EPSPS activity in glyphosate resistant plants was associated with a 15-fold higher expression of EPSPS compared with susceptible plants. Therefore, the over-expression of EPSPS appears to be the main mechanism responsible for resistance to glyphosate. This mechanism has a constitutive character and has important effects on plant fitness, as recently reported.
publishDate 2017
dc.date.none.fl_str_mv 2017-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/66847
Yanniccari, Marcos Ezequiel; Gómez Lobato, María Eugenia; Istilart, Carolina María; Natalucci, Claudia Luisa; Gimenez, Daniel Oscar; et al.; Mechanism of resistance to glyphosate in Lolium perenne from Argentina; Frontiers Media S. A; Frontiers in Ecology and Evolution; 5; OCT; 10-2017; 1-8
2296-701X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/66847
identifier_str_mv Yanniccari, Marcos Ezequiel; Gómez Lobato, María Eugenia; Istilart, Carolina María; Natalucci, Claudia Luisa; Gimenez, Daniel Oscar; et al.; Mechanism of resistance to glyphosate in Lolium perenne from Argentina; Frontiers Media S. A; Frontiers in Ecology and Evolution; 5; OCT; 10-2017; 1-8
2296-701X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fevo.2017.00123
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fevo.2017.00123
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S. A
publisher.none.fl_str_mv Frontiers Media S. A
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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