Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula
- Autores
- Vázquez García, José G; Rojano Delgado, Antonia María; Alcántara de la Cruz, Ricardo; Torra, Joel; Dellaferrera, Ignacio Miguel; Portugal, João; De Prado, Rafael
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The levels of resistance to glyphosate of 13 barnyard grass (Echinochloa crus-galli) populations harvested across different agriculture areas in the Southern Iberian Peninsula were determined in greenhouse and laboratory experiments. Shikimate accumulation fast screening separated the populations regarding resistance to glyphosate: susceptible (S) E2, E3, E4, and E6 and resistant (R) E1, E5, E7, E8, E9, E10, E11, E12, and E13. However, resistance factor (GR50 E1–E13/GR50 E6) values separated these populations into three groups: (S) E2, E3, E4, and E6, (R) E1, E5, E7, E8, and E9, and very resistant (VR) E10, E11, E12, and E13. 14C-glyphosate assays performed on two S populations (E2 and E6) showed greater absorption and translocation than those found for R (E7 and E9) and VR (E10 and E12) populations. No previous population metabolized glyphosate to amino methyl phosphonic acid (AMPA) and glyoxylate, except for the E10 population that metabolized 51% to non-toxic products. The VR populations showed two times more 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) activity without herbicide than the rest, while the inhibition of the EPSPS activity by 50% (I50) required much higher glyphosate in R and VR populations than in S populations. These results indicated that different target-site and non-target-site resistance mechanisms were implicated in the resistance to glyphosate in E. crus-galli. Our results conclude that resistance is independent of climate, type of crop, and geographic region and that the level of glyphosate resistance was mainly due to the selection pressure made by the herbicide on the different populations of E. crus-galli studied.
Fil: Vázquez García, José G. Universidad de Córdoba; España
Fil: Rojano Delgado, Antonia María. Universidad de Córdoba; España
Fil: Alcántara de la Cruz, Ricardo. Universidade Federal do São Carlos; Brasil
Fil: Torra, Joel. Universidad de Lleida; España
Fil: Dellaferrera, Ignacio Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina
Fil: Portugal, João. Instituto Politcnico de Beja; Portugal. Instituto Politcnico de Portalegre; Portugal
Fil: De Prado, Rafael. Universidad de Córdoba; España - Materia
-
BARNYARD GRASS
ENHANCED METABOLISM
GLYPHOSATE
NON-TARGET-SITE RESISTANCE (NTSR)
RESISTANCE MECHANISMS
TARGET-SITE RESISTANCE (TSR) - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/171789
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Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian PeninsulaVázquez García, José GRojano Delgado, Antonia MaríaAlcántara de la Cruz, RicardoTorra, JoelDellaferrera, Ignacio MiguelPortugal, JoãoDe Prado, RafaelBARNYARD GRASSENHANCED METABOLISMGLYPHOSATENON-TARGET-SITE RESISTANCE (NTSR)RESISTANCE MECHANISMSTARGET-SITE RESISTANCE (TSR)https://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4The levels of resistance to glyphosate of 13 barnyard grass (Echinochloa crus-galli) populations harvested across different agriculture areas in the Southern Iberian Peninsula were determined in greenhouse and laboratory experiments. Shikimate accumulation fast screening separated the populations regarding resistance to glyphosate: susceptible (S) E2, E3, E4, and E6 and resistant (R) E1, E5, E7, E8, E9, E10, E11, E12, and E13. However, resistance factor (GR50 E1–E13/GR50 E6) values separated these populations into three groups: (S) E2, E3, E4, and E6, (R) E1, E5, E7, E8, and E9, and very resistant (VR) E10, E11, E12, and E13. 14C-glyphosate assays performed on two S populations (E2 and E6) showed greater absorption and translocation than those found for R (E7 and E9) and VR (E10 and E12) populations. No previous population metabolized glyphosate to amino methyl phosphonic acid (AMPA) and glyoxylate, except for the E10 population that metabolized 51% to non-toxic products. The VR populations showed two times more 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) activity without herbicide than the rest, while the inhibition of the EPSPS activity by 50% (I50) required much higher glyphosate in R and VR populations than in S populations. These results indicated that different target-site and non-target-site resistance mechanisms were implicated in the resistance to glyphosate in E. crus-galli. Our results conclude that resistance is independent of climate, type of crop, and geographic region and that the level of glyphosate resistance was mainly due to the selection pressure made by the herbicide on the different populations of E. crus-galli studied.Fil: Vázquez García, José G. Universidad de Córdoba; EspañaFil: Rojano Delgado, Antonia María. Universidad de Córdoba; EspañaFil: Alcántara de la Cruz, Ricardo. Universidade Federal do São Carlos; BrasilFil: Torra, Joel. Universidad de Lleida; EspañaFil: Dellaferrera, Ignacio Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; ArgentinaFil: Portugal, João. Instituto Politcnico de Beja; Portugal. Instituto Politcnico de Portalegre; PortugalFil: De Prado, Rafael. Universidad de Córdoba; EspañaFrontiers Media2021-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/171789Vázquez García, José G; Rojano Delgado, Antonia María; Alcántara de la Cruz, Ricardo; Torra, Joel; Dellaferrera, Ignacio Miguel; et al.; Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula; Frontiers Media; Frontiers in Plant Science; 12; 2-2021; 1-121664-462XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2021.617040info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:30:47Zoai:ri.conicet.gov.ar:11336/171789instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:30:48.019CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula |
title |
Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula |
spellingShingle |
Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula Vázquez García, José G BARNYARD GRASS ENHANCED METABOLISM GLYPHOSATE NON-TARGET-SITE RESISTANCE (NTSR) RESISTANCE MECHANISMS TARGET-SITE RESISTANCE (TSR) |
title_short |
Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula |
title_full |
Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula |
title_fullStr |
Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula |
title_full_unstemmed |
Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula |
title_sort |
Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula |
dc.creator.none.fl_str_mv |
Vázquez García, José G Rojano Delgado, Antonia María Alcántara de la Cruz, Ricardo Torra, Joel Dellaferrera, Ignacio Miguel Portugal, João De Prado, Rafael |
author |
Vázquez García, José G |
author_facet |
Vázquez García, José G Rojano Delgado, Antonia María Alcántara de la Cruz, Ricardo Torra, Joel Dellaferrera, Ignacio Miguel Portugal, João De Prado, Rafael |
author_role |
author |
author2 |
Rojano Delgado, Antonia María Alcántara de la Cruz, Ricardo Torra, Joel Dellaferrera, Ignacio Miguel Portugal, João De Prado, Rafael |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
BARNYARD GRASS ENHANCED METABOLISM GLYPHOSATE NON-TARGET-SITE RESISTANCE (NTSR) RESISTANCE MECHANISMS TARGET-SITE RESISTANCE (TSR) |
topic |
BARNYARD GRASS ENHANCED METABOLISM GLYPHOSATE NON-TARGET-SITE RESISTANCE (NTSR) RESISTANCE MECHANISMS TARGET-SITE RESISTANCE (TSR) |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.1 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
The levels of resistance to glyphosate of 13 barnyard grass (Echinochloa crus-galli) populations harvested across different agriculture areas in the Southern Iberian Peninsula were determined in greenhouse and laboratory experiments. Shikimate accumulation fast screening separated the populations regarding resistance to glyphosate: susceptible (S) E2, E3, E4, and E6 and resistant (R) E1, E5, E7, E8, E9, E10, E11, E12, and E13. However, resistance factor (GR50 E1–E13/GR50 E6) values separated these populations into three groups: (S) E2, E3, E4, and E6, (R) E1, E5, E7, E8, and E9, and very resistant (VR) E10, E11, E12, and E13. 14C-glyphosate assays performed on two S populations (E2 and E6) showed greater absorption and translocation than those found for R (E7 and E9) and VR (E10 and E12) populations. No previous population metabolized glyphosate to amino methyl phosphonic acid (AMPA) and glyoxylate, except for the E10 population that metabolized 51% to non-toxic products. The VR populations showed two times more 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) activity without herbicide than the rest, while the inhibition of the EPSPS activity by 50% (I50) required much higher glyphosate in R and VR populations than in S populations. These results indicated that different target-site and non-target-site resistance mechanisms were implicated in the resistance to glyphosate in E. crus-galli. Our results conclude that resistance is independent of climate, type of crop, and geographic region and that the level of glyphosate resistance was mainly due to the selection pressure made by the herbicide on the different populations of E. crus-galli studied. Fil: Vázquez García, José G. Universidad de Córdoba; España Fil: Rojano Delgado, Antonia María. Universidad de Córdoba; España Fil: Alcántara de la Cruz, Ricardo. Universidade Federal do São Carlos; Brasil Fil: Torra, Joel. Universidad de Lleida; España Fil: Dellaferrera, Ignacio Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina Fil: Portugal, João. Instituto Politcnico de Beja; Portugal. Instituto Politcnico de Portalegre; Portugal Fil: De Prado, Rafael. Universidad de Córdoba; España |
description |
The levels of resistance to glyphosate of 13 barnyard grass (Echinochloa crus-galli) populations harvested across different agriculture areas in the Southern Iberian Peninsula were determined in greenhouse and laboratory experiments. Shikimate accumulation fast screening separated the populations regarding resistance to glyphosate: susceptible (S) E2, E3, E4, and E6 and resistant (R) E1, E5, E7, E8, E9, E10, E11, E12, and E13. However, resistance factor (GR50 E1–E13/GR50 E6) values separated these populations into three groups: (S) E2, E3, E4, and E6, (R) E1, E5, E7, E8, and E9, and very resistant (VR) E10, E11, E12, and E13. 14C-glyphosate assays performed on two S populations (E2 and E6) showed greater absorption and translocation than those found for R (E7 and E9) and VR (E10 and E12) populations. No previous population metabolized glyphosate to amino methyl phosphonic acid (AMPA) and glyoxylate, except for the E10 population that metabolized 51% to non-toxic products. The VR populations showed two times more 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) activity without herbicide than the rest, while the inhibition of the EPSPS activity by 50% (I50) required much higher glyphosate in R and VR populations than in S populations. These results indicated that different target-site and non-target-site resistance mechanisms were implicated in the resistance to glyphosate in E. crus-galli. Our results conclude that resistance is independent of climate, type of crop, and geographic region and that the level of glyphosate resistance was mainly due to the selection pressure made by the herbicide on the different populations of E. crus-galli studied. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/171789 Vázquez García, José G; Rojano Delgado, Antonia María; Alcántara de la Cruz, Ricardo; Torra, Joel; Dellaferrera, Ignacio Miguel; et al.; Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula; Frontiers Media; Frontiers in Plant Science; 12; 2-2021; 1-12 1664-462X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/171789 |
identifier_str_mv |
Vázquez García, José G; Rojano Delgado, Antonia María; Alcántara de la Cruz, Ricardo; Torra, Joel; Dellaferrera, Ignacio Miguel; et al.; Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula; Frontiers Media; Frontiers in Plant Science; 12; 2-2021; 1-12 1664-462X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2021.617040 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media |
publisher.none.fl_str_mv |
Frontiers Media |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |