Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula

Autores
Vázquez García, José G; Rojano Delgado, Antonia María; Alcántara de la Cruz, Ricardo; Torra, Joel; Dellaferrera, Ignacio Miguel; Portugal, João; De Prado, Rafael
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The levels of resistance to glyphosate of 13 barnyard grass (Echinochloa crus-galli) populations harvested across different agriculture areas in the Southern Iberian Peninsula were determined in greenhouse and laboratory experiments. Shikimate accumulation fast screening separated the populations regarding resistance to glyphosate: susceptible (S) E2, E3, E4, and E6 and resistant (R) E1, E5, E7, E8, E9, E10, E11, E12, and E13. However, resistance factor (GR50 E1–E13/GR50 E6) values separated these populations into three groups: (S) E2, E3, E4, and E6, (R) E1, E5, E7, E8, and E9, and very resistant (VR) E10, E11, E12, and E13. 14C-glyphosate assays performed on two S populations (E2 and E6) showed greater absorption and translocation than those found for R (E7 and E9) and VR (E10 and E12) populations. No previous population metabolized glyphosate to amino methyl phosphonic acid (AMPA) and glyoxylate, except for the E10 population that metabolized 51% to non-toxic products. The VR populations showed two times more 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) activity without herbicide than the rest, while the inhibition of the EPSPS activity by 50% (I50) required much higher glyphosate in R and VR populations than in S populations. These results indicated that different target-site and non-target-site resistance mechanisms were implicated in the resistance to glyphosate in E. crus-galli. Our results conclude that resistance is independent of climate, type of crop, and geographic region and that the level of glyphosate resistance was mainly due to the selection pressure made by the herbicide on the different populations of E. crus-galli studied.
Fil: Vázquez García, José G. Universidad de Córdoba; España
Fil: Rojano Delgado, Antonia María. Universidad de Córdoba; España
Fil: Alcántara de la Cruz, Ricardo. Universidade Federal do São Carlos; Brasil
Fil: Torra, Joel. Universidad de Lleida; España
Fil: Dellaferrera, Ignacio Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina
Fil: Portugal, João. Instituto Politcnico de Beja; Portugal. Instituto Politcnico de Portalegre; Portugal
Fil: De Prado, Rafael. Universidad de Córdoba; España
Materia
BARNYARD GRASS
ENHANCED METABOLISM
GLYPHOSATE
NON-TARGET-SITE RESISTANCE (NTSR)
RESISTANCE MECHANISMS
TARGET-SITE RESISTANCE (TSR)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/171789
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/171789
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian PeninsulaVázquez García, José GRojano Delgado, Antonia MaríaAlcántara de la Cruz, RicardoTorra, JoelDellaferrera, Ignacio MiguelPortugal, JoãoDe Prado, RafaelBARNYARD GRASSENHANCED METABOLISMGLYPHOSATENON-TARGET-SITE RESISTANCE (NTSR)RESISTANCE MECHANISMSTARGET-SITE RESISTANCE (TSR)https://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4The levels of resistance to glyphosate of 13 barnyard grass (Echinochloa crus-galli) populations harvested across different agriculture areas in the Southern Iberian Peninsula were determined in greenhouse and laboratory experiments. Shikimate accumulation fast screening separated the populations regarding resistance to glyphosate: susceptible (S) E2, E3, E4, and E6 and resistant (R) E1, E5, E7, E8, E9, E10, E11, E12, and E13. However, resistance factor (GR50 E1–E13/GR50 E6) values separated these populations into three groups: (S) E2, E3, E4, and E6, (R) E1, E5, E7, E8, and E9, and very resistant (VR) E10, E11, E12, and E13. 14C-glyphosate assays performed on two S populations (E2 and E6) showed greater absorption and translocation than those found for R (E7 and E9) and VR (E10 and E12) populations. No previous population metabolized glyphosate to amino methyl phosphonic acid (AMPA) and glyoxylate, except for the E10 population that metabolized 51% to non-toxic products. The VR populations showed two times more 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) activity without herbicide than the rest, while the inhibition of the EPSPS activity by 50% (I50) required much higher glyphosate in R and VR populations than in S populations. These results indicated that different target-site and non-target-site resistance mechanisms were implicated in the resistance to glyphosate in E. crus-galli. Our results conclude that resistance is independent of climate, type of crop, and geographic region and that the level of glyphosate resistance was mainly due to the selection pressure made by the herbicide on the different populations of E. crus-galli studied.Fil: Vázquez García, José G. Universidad de Córdoba; EspañaFil: Rojano Delgado, Antonia María. Universidad de Córdoba; EspañaFil: Alcántara de la Cruz, Ricardo. Universidade Federal do São Carlos; BrasilFil: Torra, Joel. Universidad de Lleida; EspañaFil: Dellaferrera, Ignacio Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; ArgentinaFil: Portugal, João. Instituto Politcnico de Beja; Portugal. Instituto Politcnico de Portalegre; PortugalFil: De Prado, Rafael. Universidad de Córdoba; EspañaFrontiers Media2021-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/171789Vázquez García, José G; Rojano Delgado, Antonia María; Alcántara de la Cruz, Ricardo; Torra, Joel; Dellaferrera, Ignacio Miguel; et al.; Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula; Frontiers Media; Frontiers in Plant Science; 12; 2-2021; 1-121664-462XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2021.617040info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:03:11Zoai:ri.conicet.gov.ar:11336/171789instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:03:12.004CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula
title Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula
spellingShingle Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula
Vázquez García, José G
BARNYARD GRASS
ENHANCED METABOLISM
GLYPHOSATE
NON-TARGET-SITE RESISTANCE (NTSR)
RESISTANCE MECHANISMS
TARGET-SITE RESISTANCE (TSR)
title_short Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula
title_full Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula
title_fullStr Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula
title_full_unstemmed Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula
title_sort Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula
dc.creator.none.fl_str_mv Vázquez García, José G
Rojano Delgado, Antonia María
Alcántara de la Cruz, Ricardo
Torra, Joel
Dellaferrera, Ignacio Miguel
Portugal, João
De Prado, Rafael
author Vázquez García, José G
author_facet Vázquez García, José G
Rojano Delgado, Antonia María
Alcántara de la Cruz, Ricardo
Torra, Joel
Dellaferrera, Ignacio Miguel
Portugal, João
De Prado, Rafael
author_role author
author2 Rojano Delgado, Antonia María
Alcántara de la Cruz, Ricardo
Torra, Joel
Dellaferrera, Ignacio Miguel
Portugal, João
De Prado, Rafael
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BARNYARD GRASS
ENHANCED METABOLISM
GLYPHOSATE
NON-TARGET-SITE RESISTANCE (NTSR)
RESISTANCE MECHANISMS
TARGET-SITE RESISTANCE (TSR)
topic BARNYARD GRASS
ENHANCED METABOLISM
GLYPHOSATE
NON-TARGET-SITE RESISTANCE (NTSR)
RESISTANCE MECHANISMS
TARGET-SITE RESISTANCE (TSR)
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The levels of resistance to glyphosate of 13 barnyard grass (Echinochloa crus-galli) populations harvested across different agriculture areas in the Southern Iberian Peninsula were determined in greenhouse and laboratory experiments. Shikimate accumulation fast screening separated the populations regarding resistance to glyphosate: susceptible (S) E2, E3, E4, and E6 and resistant (R) E1, E5, E7, E8, E9, E10, E11, E12, and E13. However, resistance factor (GR50 E1–E13/GR50 E6) values separated these populations into three groups: (S) E2, E3, E4, and E6, (R) E1, E5, E7, E8, and E9, and very resistant (VR) E10, E11, E12, and E13. 14C-glyphosate assays performed on two S populations (E2 and E6) showed greater absorption and translocation than those found for R (E7 and E9) and VR (E10 and E12) populations. No previous population metabolized glyphosate to amino methyl phosphonic acid (AMPA) and glyoxylate, except for the E10 population that metabolized 51% to non-toxic products. The VR populations showed two times more 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) activity without herbicide than the rest, while the inhibition of the EPSPS activity by 50% (I50) required much higher glyphosate in R and VR populations than in S populations. These results indicated that different target-site and non-target-site resistance mechanisms were implicated in the resistance to glyphosate in E. crus-galli. Our results conclude that resistance is independent of climate, type of crop, and geographic region and that the level of glyphosate resistance was mainly due to the selection pressure made by the herbicide on the different populations of E. crus-galli studied.
Fil: Vázquez García, José G. Universidad de Córdoba; España
Fil: Rojano Delgado, Antonia María. Universidad de Córdoba; España
Fil: Alcántara de la Cruz, Ricardo. Universidade Federal do São Carlos; Brasil
Fil: Torra, Joel. Universidad de Lleida; España
Fil: Dellaferrera, Ignacio Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina
Fil: Portugal, João. Instituto Politcnico de Beja; Portugal. Instituto Politcnico de Portalegre; Portugal
Fil: De Prado, Rafael. Universidad de Córdoba; España
description The levels of resistance to glyphosate of 13 barnyard grass (Echinochloa crus-galli) populations harvested across different agriculture areas in the Southern Iberian Peninsula were determined in greenhouse and laboratory experiments. Shikimate accumulation fast screening separated the populations regarding resistance to glyphosate: susceptible (S) E2, E3, E4, and E6 and resistant (R) E1, E5, E7, E8, E9, E10, E11, E12, and E13. However, resistance factor (GR50 E1–E13/GR50 E6) values separated these populations into three groups: (S) E2, E3, E4, and E6, (R) E1, E5, E7, E8, and E9, and very resistant (VR) E10, E11, E12, and E13. 14C-glyphosate assays performed on two S populations (E2 and E6) showed greater absorption and translocation than those found for R (E7 and E9) and VR (E10 and E12) populations. No previous population metabolized glyphosate to amino methyl phosphonic acid (AMPA) and glyoxylate, except for the E10 population that metabolized 51% to non-toxic products. The VR populations showed two times more 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) activity without herbicide than the rest, while the inhibition of the EPSPS activity by 50% (I50) required much higher glyphosate in R and VR populations than in S populations. These results indicated that different target-site and non-target-site resistance mechanisms were implicated in the resistance to glyphosate in E. crus-galli. Our results conclude that resistance is independent of climate, type of crop, and geographic region and that the level of glyphosate resistance was mainly due to the selection pressure made by the herbicide on the different populations of E. crus-galli studied.
publishDate 2021
dc.date.none.fl_str_mv 2021-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/171789
Vázquez García, José G; Rojano Delgado, Antonia María; Alcántara de la Cruz, Ricardo; Torra, Joel; Dellaferrera, Ignacio Miguel; et al.; Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula; Frontiers Media; Frontiers in Plant Science; 12; 2-2021; 1-12
1664-462X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/171789
identifier_str_mv Vázquez García, José G; Rojano Delgado, Antonia María; Alcántara de la Cruz, Ricardo; Torra, Joel; Dellaferrera, Ignacio Miguel; et al.; Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula; Frontiers Media; Frontiers in Plant Science; 12; 2-2021; 1-12
1664-462X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2021.617040
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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