On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions

Autores
Vissani, María Aldana; Tordoya, Maria Silvia; Tsai, Y.-L.; Lee, P.-Y.A.; Shen, Y.-H.; Lee, F.-C.; Wang, H.-T.T.; Parreño, Gladys Viviana; Barrandeguy, María Edith
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Equine coital exanthema (ECE) is an infectious, venereally transmitted muco-cutaneous disease affecting mares and stallions, caused by equid alphaherpesvirus 3 (EHV3). Diagnostic tools for rapid identification of EHV3 are of primary importance to diminish the risk of EHV3 dissemination at the time of breeding. In the last years, it has been shown that the performance of the insulated-isothermal polymerase chain reaction (iiPCR) is comparable to virus isolation, nested PCR and real-time PCR (qPCR) in detecting pathogens of various animal species. Analytical sensitivity and specificity of the iiPCR were compared with a qPCR, using a plasmid containing the target region of the EHV3 glycoprotein G gene and an Argentinian EHV3 isolate (E/9283/07 C3A). In order to evaluate the diagnostic performance of the iiPCR, nucleic acids of 85 perineal and genital swabs (PGS) of mares and stallions were extracted by tacoTM mini and tested by both techniques. EHV3 was detected in 46 and 45 of the 85 PGS by the iiPCR and qPCR, respectively. There was almost perfect agreement between the two diagnostic methods (98.82%; 95% CI: 95.03–100%; κ = 0.98). The iiPCR had a limit of detection of 95.00% at 6 genome equivalents per reaction and a detection endpoint for viral DNA comparable to that of the qPCR, and did not react with six non-targeted equine pathogens. The iiPCR represents a sensitive and specific method for the rapid on-site diagnosis of EHV3 infection. Its routinely implementation in breeding facilities, and artificial insemination and embryo transfer centers, will contribute to prevent the dissemination of this venereal, highly contagious disease in horses.
Fil: Vissani, María Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Tordoya, Maria Silvia. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Tsai, Y.-L.. GeneReach; Estados Unidos
Fil: Lee, P.-Y.A.. GeneReach; Estados Unidos
Fil: Shen, Y.-H.. GeneReach; Estados Unidos
Fil: Lee, F.-C.. GeneReach; Estados Unidos
Fil: Wang, H.-T.T.. GeneReach; Estados Unidos
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barrandeguy, María Edith. Instituto Nacional de Tecnología Agropecuaria; Argentina. Universidad del Salvador. Escuela de Veterinaria; Argentina
Materia
ECE
EQUID HERPESVIRUS 3
IIPCR
ON-SITE DETECTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/99245
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/99245
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallionsVissani, María AldanaTordoya, Maria SilviaTsai, Y.-L.Lee, P.-Y.A.Shen, Y.-H.Lee, F.-C.Wang, H.-T.T.Parreño, Gladys VivianaBarrandeguy, María EdithECEEQUID HERPESVIRUS 3IIPCRON-SITE DETECTIONhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Equine coital exanthema (ECE) is an infectious, venereally transmitted muco-cutaneous disease affecting mares and stallions, caused by equid alphaherpesvirus 3 (EHV3). Diagnostic tools for rapid identification of EHV3 are of primary importance to diminish the risk of EHV3 dissemination at the time of breeding. In the last years, it has been shown that the performance of the insulated-isothermal polymerase chain reaction (iiPCR) is comparable to virus isolation, nested PCR and real-time PCR (qPCR) in detecting pathogens of various animal species. Analytical sensitivity and specificity of the iiPCR were compared with a qPCR, using a plasmid containing the target region of the EHV3 glycoprotein G gene and an Argentinian EHV3 isolate (E/9283/07 C3A). In order to evaluate the diagnostic performance of the iiPCR, nucleic acids of 85 perineal and genital swabs (PGS) of mares and stallions were extracted by tacoTM mini and tested by both techniques. EHV3 was detected in 46 and 45 of the 85 PGS by the iiPCR and qPCR, respectively. There was almost perfect agreement between the two diagnostic methods (98.82%; 95% CI: 95.03–100%; κ = 0.98). The iiPCR had a limit of detection of 95.00% at 6 genome equivalents per reaction and a detection endpoint for viral DNA comparable to that of the qPCR, and did not react with six non-targeted equine pathogens. The iiPCR represents a sensitive and specific method for the rapid on-site diagnosis of EHV3 infection. Its routinely implementation in breeding facilities, and artificial insemination and embryo transfer centers, will contribute to prevent the dissemination of this venereal, highly contagious disease in horses.Fil: Vissani, María Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Tordoya, Maria Silvia. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Tsai, Y.-L.. GeneReach; Estados UnidosFil: Lee, P.-Y.A.. GeneReach; Estados UnidosFil: Shen, Y.-H.. GeneReach; Estados UnidosFil: Lee, F.-C.. GeneReach; Estados UnidosFil: Wang, H.-T.T.. GeneReach; Estados UnidosFil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barrandeguy, María Edith. Instituto Nacional de Tecnología Agropecuaria; Argentina. Universidad del Salvador. Escuela de Veterinaria; ArgentinaElsevier Science2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/99245Vissani, María Aldana; Tordoya, Maria Silvia; Tsai, Y.-L.; Lee, P.-Y.A.; Shen, Y.-H.; et al.; On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions; Elsevier Science; Journal of Virological Methods; 257; 7-2018; 29-320166-0934CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0166093418300260info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jviromet.2018.04.002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:38Zoai:ri.conicet.gov.ar:11336/99245instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:39.073CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions
title On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions
spellingShingle On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions
Vissani, María Aldana
ECE
EQUID HERPESVIRUS 3
IIPCR
ON-SITE DETECTION
title_short On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions
title_full On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions
title_fullStr On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions
title_full_unstemmed On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions
title_sort On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions
dc.creator.none.fl_str_mv Vissani, María Aldana
Tordoya, Maria Silvia
Tsai, Y.-L.
Lee, P.-Y.A.
Shen, Y.-H.
Lee, F.-C.
Wang, H.-T.T.
Parreño, Gladys Viviana
Barrandeguy, María Edith
author Vissani, María Aldana
author_facet Vissani, María Aldana
Tordoya, Maria Silvia
Tsai, Y.-L.
Lee, P.-Y.A.
Shen, Y.-H.
Lee, F.-C.
Wang, H.-T.T.
Parreño, Gladys Viviana
Barrandeguy, María Edith
author_role author
author2 Tordoya, Maria Silvia
Tsai, Y.-L.
Lee, P.-Y.A.
Shen, Y.-H.
Lee, F.-C.
Wang, H.-T.T.
Parreño, Gladys Viviana
Barrandeguy, María Edith
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ECE
EQUID HERPESVIRUS 3
IIPCR
ON-SITE DETECTION
topic ECE
EQUID HERPESVIRUS 3
IIPCR
ON-SITE DETECTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Equine coital exanthema (ECE) is an infectious, venereally transmitted muco-cutaneous disease affecting mares and stallions, caused by equid alphaherpesvirus 3 (EHV3). Diagnostic tools for rapid identification of EHV3 are of primary importance to diminish the risk of EHV3 dissemination at the time of breeding. In the last years, it has been shown that the performance of the insulated-isothermal polymerase chain reaction (iiPCR) is comparable to virus isolation, nested PCR and real-time PCR (qPCR) in detecting pathogens of various animal species. Analytical sensitivity and specificity of the iiPCR were compared with a qPCR, using a plasmid containing the target region of the EHV3 glycoprotein G gene and an Argentinian EHV3 isolate (E/9283/07 C3A). In order to evaluate the diagnostic performance of the iiPCR, nucleic acids of 85 perineal and genital swabs (PGS) of mares and stallions were extracted by tacoTM mini and tested by both techniques. EHV3 was detected in 46 and 45 of the 85 PGS by the iiPCR and qPCR, respectively. There was almost perfect agreement between the two diagnostic methods (98.82%; 95% CI: 95.03–100%; κ = 0.98). The iiPCR had a limit of detection of 95.00% at 6 genome equivalents per reaction and a detection endpoint for viral DNA comparable to that of the qPCR, and did not react with six non-targeted equine pathogens. The iiPCR represents a sensitive and specific method for the rapid on-site diagnosis of EHV3 infection. Its routinely implementation in breeding facilities, and artificial insemination and embryo transfer centers, will contribute to prevent the dissemination of this venereal, highly contagious disease in horses.
Fil: Vissani, María Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Tordoya, Maria Silvia. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Tsai, Y.-L.. GeneReach; Estados Unidos
Fil: Lee, P.-Y.A.. GeneReach; Estados Unidos
Fil: Shen, Y.-H.. GeneReach; Estados Unidos
Fil: Lee, F.-C.. GeneReach; Estados Unidos
Fil: Wang, H.-T.T.. GeneReach; Estados Unidos
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barrandeguy, María Edith. Instituto Nacional de Tecnología Agropecuaria; Argentina. Universidad del Salvador. Escuela de Veterinaria; Argentina
description Equine coital exanthema (ECE) is an infectious, venereally transmitted muco-cutaneous disease affecting mares and stallions, caused by equid alphaherpesvirus 3 (EHV3). Diagnostic tools for rapid identification of EHV3 are of primary importance to diminish the risk of EHV3 dissemination at the time of breeding. In the last years, it has been shown that the performance of the insulated-isothermal polymerase chain reaction (iiPCR) is comparable to virus isolation, nested PCR and real-time PCR (qPCR) in detecting pathogens of various animal species. Analytical sensitivity and specificity of the iiPCR were compared with a qPCR, using a plasmid containing the target region of the EHV3 glycoprotein G gene and an Argentinian EHV3 isolate (E/9283/07 C3A). In order to evaluate the diagnostic performance of the iiPCR, nucleic acids of 85 perineal and genital swabs (PGS) of mares and stallions were extracted by tacoTM mini and tested by both techniques. EHV3 was detected in 46 and 45 of the 85 PGS by the iiPCR and qPCR, respectively. There was almost perfect agreement between the two diagnostic methods (98.82%; 95% CI: 95.03–100%; κ = 0.98). The iiPCR had a limit of detection of 95.00% at 6 genome equivalents per reaction and a detection endpoint for viral DNA comparable to that of the qPCR, and did not react with six non-targeted equine pathogens. The iiPCR represents a sensitive and specific method for the rapid on-site diagnosis of EHV3 infection. Its routinely implementation in breeding facilities, and artificial insemination and embryo transfer centers, will contribute to prevent the dissemination of this venereal, highly contagious disease in horses.
publishDate 2018
dc.date.none.fl_str_mv 2018-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/99245
Vissani, María Aldana; Tordoya, Maria Silvia; Tsai, Y.-L.; Lee, P.-Y.A.; Shen, Y.-H.; et al.; On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions; Elsevier Science; Journal of Virological Methods; 257; 7-2018; 29-32
0166-0934
CONICET Digital
CONICET
url http://hdl.handle.net/11336/99245
identifier_str_mv Vissani, María Aldana; Tordoya, Maria Silvia; Tsai, Y.-L.; Lee, P.-Y.A.; Shen, Y.-H.; et al.; On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions; Elsevier Science; Journal of Virological Methods; 257; 7-2018; 29-32
0166-0934
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0166093418300260
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jviromet.2018.04.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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