On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions
- Autores
- Vissani, Maria Aldana; Tordoya, Maria Silvia; Tsai, Yun-Long; Lee, Pei-Yu Alison; Shen, Yu-Han; Lee, Fu-Chun; Wang, Hwa-Tang Thomas; Parreño, Viviana; Barrandeguy, Maria Edith
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Equine coital exanthema (ECE) is an infectious, venereally transmitted muco-cutaneous disease affecting mares and stallions, caused by equid alphaherpesvirus 3 (EHV3). Diagnostic tools for rapid identification of EHV3 are of primary importance to diminish the risk of EHV3 dissemination at the time of breeding. In the last years, it has been shown that the performance of the insulated-isothermal polymerase chain reaction (iiPCR) is comparable to virus isolation, nested PCR and real-time PCR (qPCR) in detecting pathogens of various animal species. Analytical sensitivity and specificity of the iiPCR were compared with a qPCR, using a plasmid containing the target region of the EHV3 glycoprotein G gene and an Argentinian EHV3 isolate (E/9283/07 C3A). In order to evaluate the diagnostic performance of the iiPCR, nucleic acids of 85 perineal and genital swabs (PGS) of mares and stallions were extracted by tacoTM mini and tested by both techniques. EHV3 was detected in 46 and 45 of the 85 PGS by the iiPCR and qPCR, respectively. There was almost perfect agreement between the two diagnostic methods (98.82%; 95% CI: 95.03–100%; κ = 0.98). The iiPCR had a limit of detection of 95.00% at 6 genome equivalents per reaction and a detection endpoint for viral DNA comparable to that of the qPCR, and did not react with six non-targeted equine pathogens. The iiPCR represents a sensitive and specific method for the rapid on-site diagnosis of EHV3 infection. Its routinely implementation in breeding facilities, and artificial insemination and embryo transfer centers, will contribute to prevent the dissemination of this venereal, highly contagious disease in horses.
Instituto de Virología
Fil: Vissani, Aldana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Tordoya, Maria Silvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Tsai, Yun-Long. GeneReach USA, Lexington; Estados Unidos
Fil: Lee, Pei-Yu Alison. GeneReach USA, Lexington; Estados Unidos
Fil: Shen, Yu-Han. GeneReach USA, Lexington; Estados Unidos
Fil: Lee, Fu-Chun. GeneReach USA, Lexington; Estados Unidos
Fil: Wang, Hwa-Tang Thomas. GeneReach USA, Lexington; Estados Unidos
Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentina - Fuente
- Journal of virological methods 257 : 29-32. (July 2018)
- Materia
-
Herpesviridae
Exantema Coital
Yegua
Animal Reproductor
PCR
Coital Exanthema
Mares
Breeding Sbtock
Equid Herpesvirus
Detección In Situ
Equinos
Equine Coital Exanthema
Insulated-isothermal Polymerase Chain Reaction
On-site Detection - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/4479
Ver los metadatos del registro completo
| id |
INTADig_8826f116352061b9281a22c109416a0b |
|---|---|
| oai_identifier_str |
oai:localhost:20.500.12123/4479 |
| network_acronym_str |
INTADig |
| repository_id_str |
l |
| network_name_str |
INTA Digital (INTA) |
| spelling |
On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallionsVissani, Maria AldanaTordoya, Maria SilviaTsai, Yun-LongLee, Pei-Yu AlisonShen, Yu-HanLee, Fu-ChunWang, Hwa-Tang ThomasParreño, VivianaBarrandeguy, Maria EdithHerpesviridaeExantema CoitalYeguaAnimal ReproductorPCRCoital ExanthemaMaresBreeding SbtockEquid HerpesvirusDetección In SituEquinosEquine Coital ExanthemaInsulated-isothermal Polymerase Chain ReactionOn-site DetectionEquine coital exanthema (ECE) is an infectious, venereally transmitted muco-cutaneous disease affecting mares and stallions, caused by equid alphaherpesvirus 3 (EHV3). Diagnostic tools for rapid identification of EHV3 are of primary importance to diminish the risk of EHV3 dissemination at the time of breeding. In the last years, it has been shown that the performance of the insulated-isothermal polymerase chain reaction (iiPCR) is comparable to virus isolation, nested PCR and real-time PCR (qPCR) in detecting pathogens of various animal species. Analytical sensitivity and specificity of the iiPCR were compared with a qPCR, using a plasmid containing the target region of the EHV3 glycoprotein G gene and an Argentinian EHV3 isolate (E/9283/07 C3A). In order to evaluate the diagnostic performance of the iiPCR, nucleic acids of 85 perineal and genital swabs (PGS) of mares and stallions were extracted by tacoTM mini and tested by both techniques. EHV3 was detected in 46 and 45 of the 85 PGS by the iiPCR and qPCR, respectively. There was almost perfect agreement between the two diagnostic methods (98.82%; 95% CI: 95.03–100%; κ = 0.98). The iiPCR had a limit of detection of 95.00% at 6 genome equivalents per reaction and a detection endpoint for viral DNA comparable to that of the qPCR, and did not react with six non-targeted equine pathogens. The iiPCR represents a sensitive and specific method for the rapid on-site diagnosis of EHV3 infection. Its routinely implementation in breeding facilities, and artificial insemination and embryo transfer centers, will contribute to prevent the dissemination of this venereal, highly contagious disease in horses.Instituto de VirologíaFil: Vissani, Aldana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Tordoya, Maria Silvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Tsai, Yun-Long. GeneReach USA, Lexington; Estados UnidosFil: Lee, Pei-Yu Alison. GeneReach USA, Lexington; Estados UnidosFil: Shen, Yu-Han. GeneReach USA, Lexington; Estados UnidosFil: Lee, Fu-Chun. GeneReach USA, Lexington; Estados UnidosFil: Wang, Hwa-Tang Thomas. GeneReach USA, Lexington; Estados UnidosFil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; ArgentinaElsevier2019-02-21T13:01:52Z2019-02-21T13:01:52Z2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4479https://www.sciencedirect.com/science/article/pii/S0166093418300260?via%3Dihub0166-0934https://doi.org/10.1016/j.jviromet.2018.04.002Journal of virological methods 257 : 29-32. (July 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:26Zoai:localhost:20.500.12123/4479instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:27.301INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions |
| title |
On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions |
| spellingShingle |
On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions Vissani, Maria Aldana Herpesviridae Exantema Coital Yegua Animal Reproductor PCR Coital Exanthema Mares Breeding Sbtock Equid Herpesvirus Detección In Situ Equinos Equine Coital Exanthema Insulated-isothermal Polymerase Chain Reaction On-site Detection |
| title_short |
On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions |
| title_full |
On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions |
| title_fullStr |
On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions |
| title_full_unstemmed |
On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions |
| title_sort |
On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions |
| dc.creator.none.fl_str_mv |
Vissani, Maria Aldana Tordoya, Maria Silvia Tsai, Yun-Long Lee, Pei-Yu Alison Shen, Yu-Han Lee, Fu-Chun Wang, Hwa-Tang Thomas Parreño, Viviana Barrandeguy, Maria Edith |
| author |
Vissani, Maria Aldana |
| author_facet |
Vissani, Maria Aldana Tordoya, Maria Silvia Tsai, Yun-Long Lee, Pei-Yu Alison Shen, Yu-Han Lee, Fu-Chun Wang, Hwa-Tang Thomas Parreño, Viviana Barrandeguy, Maria Edith |
| author_role |
author |
| author2 |
Tordoya, Maria Silvia Tsai, Yun-Long Lee, Pei-Yu Alison Shen, Yu-Han Lee, Fu-Chun Wang, Hwa-Tang Thomas Parreño, Viviana Barrandeguy, Maria Edith |
| author2_role |
author author author author author author author author |
| dc.subject.none.fl_str_mv |
Herpesviridae Exantema Coital Yegua Animal Reproductor PCR Coital Exanthema Mares Breeding Sbtock Equid Herpesvirus Detección In Situ Equinos Equine Coital Exanthema Insulated-isothermal Polymerase Chain Reaction On-site Detection |
| topic |
Herpesviridae Exantema Coital Yegua Animal Reproductor PCR Coital Exanthema Mares Breeding Sbtock Equid Herpesvirus Detección In Situ Equinos Equine Coital Exanthema Insulated-isothermal Polymerase Chain Reaction On-site Detection |
| dc.description.none.fl_txt_mv |
Equine coital exanthema (ECE) is an infectious, venereally transmitted muco-cutaneous disease affecting mares and stallions, caused by equid alphaherpesvirus 3 (EHV3). Diagnostic tools for rapid identification of EHV3 are of primary importance to diminish the risk of EHV3 dissemination at the time of breeding. In the last years, it has been shown that the performance of the insulated-isothermal polymerase chain reaction (iiPCR) is comparable to virus isolation, nested PCR and real-time PCR (qPCR) in detecting pathogens of various animal species. Analytical sensitivity and specificity of the iiPCR were compared with a qPCR, using a plasmid containing the target region of the EHV3 glycoprotein G gene and an Argentinian EHV3 isolate (E/9283/07 C3A). In order to evaluate the diagnostic performance of the iiPCR, nucleic acids of 85 perineal and genital swabs (PGS) of mares and stallions were extracted by tacoTM mini and tested by both techniques. EHV3 was detected in 46 and 45 of the 85 PGS by the iiPCR and qPCR, respectively. There was almost perfect agreement between the two diagnostic methods (98.82%; 95% CI: 95.03–100%; κ = 0.98). The iiPCR had a limit of detection of 95.00% at 6 genome equivalents per reaction and a detection endpoint for viral DNA comparable to that of the qPCR, and did not react with six non-targeted equine pathogens. The iiPCR represents a sensitive and specific method for the rapid on-site diagnosis of EHV3 infection. Its routinely implementation in breeding facilities, and artificial insemination and embryo transfer centers, will contribute to prevent the dissemination of this venereal, highly contagious disease in horses. Instituto de Virología Fil: Vissani, Aldana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Tordoya, Maria Silvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Tsai, Yun-Long. GeneReach USA, Lexington; Estados Unidos Fil: Lee, Pei-Yu Alison. GeneReach USA, Lexington; Estados Unidos Fil: Shen, Yu-Han. GeneReach USA, Lexington; Estados Unidos Fil: Lee, Fu-Chun. GeneReach USA, Lexington; Estados Unidos Fil: Wang, Hwa-Tang Thomas. GeneReach USA, Lexington; Estados Unidos Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentina |
| description |
Equine coital exanthema (ECE) is an infectious, venereally transmitted muco-cutaneous disease affecting mares and stallions, caused by equid alphaherpesvirus 3 (EHV3). Diagnostic tools for rapid identification of EHV3 are of primary importance to diminish the risk of EHV3 dissemination at the time of breeding. In the last years, it has been shown that the performance of the insulated-isothermal polymerase chain reaction (iiPCR) is comparable to virus isolation, nested PCR and real-time PCR (qPCR) in detecting pathogens of various animal species. Analytical sensitivity and specificity of the iiPCR were compared with a qPCR, using a plasmid containing the target region of the EHV3 glycoprotein G gene and an Argentinian EHV3 isolate (E/9283/07 C3A). In order to evaluate the diagnostic performance of the iiPCR, nucleic acids of 85 perineal and genital swabs (PGS) of mares and stallions were extracted by tacoTM mini and tested by both techniques. EHV3 was detected in 46 and 45 of the 85 PGS by the iiPCR and qPCR, respectively. There was almost perfect agreement between the two diagnostic methods (98.82%; 95% CI: 95.03–100%; κ = 0.98). The iiPCR had a limit of detection of 95.00% at 6 genome equivalents per reaction and a detection endpoint for viral DNA comparable to that of the qPCR, and did not react with six non-targeted equine pathogens. The iiPCR represents a sensitive and specific method for the rapid on-site diagnosis of EHV3 infection. Its routinely implementation in breeding facilities, and artificial insemination and embryo transfer centers, will contribute to prevent the dissemination of this venereal, highly contagious disease in horses. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-07 2019-02-21T13:01:52Z 2019-02-21T13:01:52Z |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/4479 https://www.sciencedirect.com/science/article/pii/S0166093418300260?via%3Dihub 0166-0934 https://doi.org/10.1016/j.jviromet.2018.04.002 |
| url |
http://hdl.handle.net/20.500.12123/4479 https://www.sciencedirect.com/science/article/pii/S0166093418300260?via%3Dihub https://doi.org/10.1016/j.jviromet.2018.04.002 |
| identifier_str_mv |
0166-0934 |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
| eu_rights_str_mv |
restrictedAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
| dc.source.none.fl_str_mv |
Journal of virological methods 257 : 29-32. (July 2018) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
| reponame_str |
INTA Digital (INTA) |
| collection |
INTA Digital (INTA) |
| instname_str |
Instituto Nacional de Tecnología Agropecuaria |
| repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
| repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
| _version_ |
1846143511159635968 |
| score |
12.712165 |