Monitoring growth and removal of Pseudomonas biofilms on cellulose-based fabrics

Autores
Agustín, María del Rosario; Stengel, Peter; Kellermeier, Matthias; Tücking, Katrin Stephanie; Müller, Mareike
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Biofilms are often tolerant towards routine cleaning and disinfection processes. As they cangrow on fabrics in household or healthcare settings, resulting in odors and serious health problems, it is necessary to contain biofilms through eradication strategies. The current study proposes a novel test model for the growth and removal of biofilms on textiles with Pseudomonas fluorescens and the opportunistic nosocomial pathogen Pseudomonas aeruginosa as model organisms. To assess the biofilm removal on fabrics, (1) a detergent-based, (2) enzyme-based, and (3) combined formulation of both detergent and enzymes (F1/2) were applied. Biofilms were analyzed microscopically (FE-SEM, SEM, 3D laser scanning- and epifluorescence microscopy), via a quartz crystal microbalance with mass dissipation monitoring (QCM-D) as well as plate counting of colonies. This study indicated that Pseudomonas spp. form robust biofilms on woven cellulose that can be efficiently removed via F1/2, proven by a significant reduction (p < 0.001) of viable bacteria in biofilms. Moreover, microscopic analysis indicated a disruption and almost complete removal of the biofilms after F1/2 treatment. QCM-D measurements further confirmed a maximal mass dissipation change after applying F1/2. The combination strategy applying both enzymes and detergent is a promising antibiofilm approach to remove bacteria from fabrics.
Fil: Agustín, María del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Stengel, Peter. Universitat Siegen;
Fil: Kellermeier, Matthias. Universitat Siegen;
Fil: Tücking, Katrin Stephanie. Universitat Siegen;
Fil: Müller, Mareike. Universitat Siegen;
Materia
BIOFILM REMOVAL
PSEUDOMONAS SPP.
CELLULOSE
TEXTILES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/229294
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Monitoring growth and removal of Pseudomonas biofilms on cellulose-based fabricsAgustín, María del RosarioStengel, PeterKellermeier, MatthiasTücking, Katrin StephanieMüller, MareikeBIOFILM REMOVALPSEUDOMONAS SPP.CELLULOSETEXTILEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Biofilms are often tolerant towards routine cleaning and disinfection processes. As they cangrow on fabrics in household or healthcare settings, resulting in odors and serious health problems, it is necessary to contain biofilms through eradication strategies. The current study proposes a novel test model for the growth and removal of biofilms on textiles with Pseudomonas fluorescens and the opportunistic nosocomial pathogen Pseudomonas aeruginosa as model organisms. To assess the biofilm removal on fabrics, (1) a detergent-based, (2) enzyme-based, and (3) combined formulation of both detergent and enzymes (F1/2) were applied. Biofilms were analyzed microscopically (FE-SEM, SEM, 3D laser scanning- and epifluorescence microscopy), via a quartz crystal microbalance with mass dissipation monitoring (QCM-D) as well as plate counting of colonies. This study indicated that Pseudomonas spp. form robust biofilms on woven cellulose that can be efficiently removed via F1/2, proven by a significant reduction (p < 0.001) of viable bacteria in biofilms. Moreover, microscopic analysis indicated a disruption and almost complete removal of the biofilms after F1/2 treatment. QCM-D measurements further confirmed a maximal mass dissipation change after applying F1/2. The combination strategy applying both enzymes and detergent is a promising antibiofilm approach to remove bacteria from fabrics.Fil: Agustín, María del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Stengel, Peter. Universitat Siegen;Fil: Kellermeier, Matthias. Universitat Siegen;Fil: Tücking, Katrin Stephanie. Universitat Siegen;Fil: Müller, Mareike. Universitat Siegen;MDPI2023-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/229294Agustín, María del Rosario; Stengel, Peter; Kellermeier, Matthias; Tücking, Katrin Stephanie; Müller, Mareike; Monitoring growth and removal of Pseudomonas biofilms on cellulose-based fabrics; MDPI; Microorganisms; 11; 4; 3-2023; 1-182076-2607CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/11/4/892info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms11040892info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:40Zoai:ri.conicet.gov.ar:11336/229294instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:40.453CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Monitoring growth and removal of Pseudomonas biofilms on cellulose-based fabrics
title Monitoring growth and removal of Pseudomonas biofilms on cellulose-based fabrics
spellingShingle Monitoring growth and removal of Pseudomonas biofilms on cellulose-based fabrics
Agustín, María del Rosario
BIOFILM REMOVAL
PSEUDOMONAS SPP.
CELLULOSE
TEXTILES
title_short Monitoring growth and removal of Pseudomonas biofilms on cellulose-based fabrics
title_full Monitoring growth and removal of Pseudomonas biofilms on cellulose-based fabrics
title_fullStr Monitoring growth and removal of Pseudomonas biofilms on cellulose-based fabrics
title_full_unstemmed Monitoring growth and removal of Pseudomonas biofilms on cellulose-based fabrics
title_sort Monitoring growth and removal of Pseudomonas biofilms on cellulose-based fabrics
dc.creator.none.fl_str_mv Agustín, María del Rosario
Stengel, Peter
Kellermeier, Matthias
Tücking, Katrin Stephanie
Müller, Mareike
author Agustín, María del Rosario
author_facet Agustín, María del Rosario
Stengel, Peter
Kellermeier, Matthias
Tücking, Katrin Stephanie
Müller, Mareike
author_role author
author2 Stengel, Peter
Kellermeier, Matthias
Tücking, Katrin Stephanie
Müller, Mareike
author2_role author
author
author
author
dc.subject.none.fl_str_mv BIOFILM REMOVAL
PSEUDOMONAS SPP.
CELLULOSE
TEXTILES
topic BIOFILM REMOVAL
PSEUDOMONAS SPP.
CELLULOSE
TEXTILES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Biofilms are often tolerant towards routine cleaning and disinfection processes. As they cangrow on fabrics in household or healthcare settings, resulting in odors and serious health problems, it is necessary to contain biofilms through eradication strategies. The current study proposes a novel test model for the growth and removal of biofilms on textiles with Pseudomonas fluorescens and the opportunistic nosocomial pathogen Pseudomonas aeruginosa as model organisms. To assess the biofilm removal on fabrics, (1) a detergent-based, (2) enzyme-based, and (3) combined formulation of both detergent and enzymes (F1/2) were applied. Biofilms were analyzed microscopically (FE-SEM, SEM, 3D laser scanning- and epifluorescence microscopy), via a quartz crystal microbalance with mass dissipation monitoring (QCM-D) as well as plate counting of colonies. This study indicated that Pseudomonas spp. form robust biofilms on woven cellulose that can be efficiently removed via F1/2, proven by a significant reduction (p < 0.001) of viable bacteria in biofilms. Moreover, microscopic analysis indicated a disruption and almost complete removal of the biofilms after F1/2 treatment. QCM-D measurements further confirmed a maximal mass dissipation change after applying F1/2. The combination strategy applying both enzymes and detergent is a promising antibiofilm approach to remove bacteria from fabrics.
Fil: Agustín, María del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Stengel, Peter. Universitat Siegen;
Fil: Kellermeier, Matthias. Universitat Siegen;
Fil: Tücking, Katrin Stephanie. Universitat Siegen;
Fil: Müller, Mareike. Universitat Siegen;
description Biofilms are often tolerant towards routine cleaning and disinfection processes. As they cangrow on fabrics in household or healthcare settings, resulting in odors and serious health problems, it is necessary to contain biofilms through eradication strategies. The current study proposes a novel test model for the growth and removal of biofilms on textiles with Pseudomonas fluorescens and the opportunistic nosocomial pathogen Pseudomonas aeruginosa as model organisms. To assess the biofilm removal on fabrics, (1) a detergent-based, (2) enzyme-based, and (3) combined formulation of both detergent and enzymes (F1/2) were applied. Biofilms were analyzed microscopically (FE-SEM, SEM, 3D laser scanning- and epifluorescence microscopy), via a quartz crystal microbalance with mass dissipation monitoring (QCM-D) as well as plate counting of colonies. This study indicated that Pseudomonas spp. form robust biofilms on woven cellulose that can be efficiently removed via F1/2, proven by a significant reduction (p < 0.001) of viable bacteria in biofilms. Moreover, microscopic analysis indicated a disruption and almost complete removal of the biofilms after F1/2 treatment. QCM-D measurements further confirmed a maximal mass dissipation change after applying F1/2. The combination strategy applying both enzymes and detergent is a promising antibiofilm approach to remove bacteria from fabrics.
publishDate 2023
dc.date.none.fl_str_mv 2023-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/229294
Agustín, María del Rosario; Stengel, Peter; Kellermeier, Matthias; Tücking, Katrin Stephanie; Müller, Mareike; Monitoring growth and removal of Pseudomonas biofilms on cellulose-based fabrics; MDPI; Microorganisms; 11; 4; 3-2023; 1-18
2076-2607
CONICET Digital
CONICET
url http://hdl.handle.net/11336/229294
identifier_str_mv Agustín, María del Rosario; Stengel, Peter; Kellermeier, Matthias; Tücking, Katrin Stephanie; Müller, Mareike; Monitoring growth and removal of Pseudomonas biofilms on cellulose-based fabrics; MDPI; Microorganisms; 11; 4; 3-2023; 1-18
2076-2607
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/11/4/892
info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms11040892
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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