Effects of Shiga toxin 2 in pregnant and non-pregnant female rats

Autores
Fischer Sigel, Lilian Karina; Sacerdoti, Flavia; Ibarra, Cristina Adriana; Zotta, Elsa; Silberstein, Claudia Marcela
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Shigatoxin-producing Escherichia colicauses acute renal failure and Hemolytic Uremic Syndrome. It was reported that inhibition of nitric oxide(NO) by Shiga toxin type 2 (Stx2) enhanced renal damage in mice and baboonmodels of Stx-mediated HUS. The aim of the work was to study the evolution ofthe damages caused by Stx2 in P compare with NP rats. Pregnant Sprague-Dawleyrats, at day 8 of gestation, and NP rats were ip inoculated with 0.5 ng Stx2/gbody weight (PS, NPS) or diluent (PC, NPC). Some PS and PC rats were treatedwith 1mg/ml L-NAME, NO inhibitor, in drinking water (PLS, PLC) from 24 h beforeip injection to 4 days post-injection (dpi). Rats were individually housed,checked for water and food intake, and weighted every 24 h until 30 dpi. At 4dpi, blood and 24 h urine samples were collected to determine urinary flow andfree water clearance (CH20). Then, rats were euthanized and kidneyswere removed for histopathological observations. NPS and PS rats showed adecrease in food intake and weight with respect to controls (p<0.05). PSrats increased food intake and recovered weight at 5 dpi, while NPS rats showedan improvement at 14 dpi. The water intake increased in NPS and PS ratscompared to controls until 7 dpi (p<0.05). In NPS at 4 dpi, the rise inwater intake coincided with an increase in urinary flow and CH20respect to NPC (p<0.05), different from what was observed in PS. The renalcortex of NPS presented significantly more necrosis and atrophied tubules thanPS (p<0.05). Preliminary results in PLS rats showed that L-NAMEsignificantly increased renal necrosis compared with PLS and PLC rats (p<0.05).In conclusion, PS rats suffered less renal damage and recovered from the Stx2effect faster than NPS rats. L-NAME increased Stx2 effect in PLS suggesting thatphysiology changes caused by pregnancy, like increasing in NO production, may contributeto protect maternal kidney from Stx2 effects.
Fil: Fischer Sigel, Lilian Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Sacerdoti, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Zotta, Elsa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Silberstein, Claudia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología.
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Materia
shiga toxin
pregnant rats
non-pregnant rats
L-NAME
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195038
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/195038
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Effects of Shiga toxin 2 in pregnant and non-pregnant female ratsFischer Sigel, Lilian KarinaSacerdoti, FlaviaIbarra, Cristina AdrianaZotta, ElsaSilberstein, Claudia Marcelashiga toxinpregnant ratsnon-pregnant ratsL-NAMEhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Shigatoxin-producing Escherichia colicauses acute renal failure and Hemolytic Uremic Syndrome. It was reported that inhibition of nitric oxide(NO) by Shiga toxin type 2 (Stx2) enhanced renal damage in mice and baboonmodels of Stx-mediated HUS. The aim of the work was to study the evolution ofthe damages caused by Stx2 in P compare with NP rats. Pregnant Sprague-Dawleyrats, at day 8 of gestation, and NP rats were ip inoculated with 0.5 ng Stx2/gbody weight (PS, NPS) or diluent (PC, NPC). Some PS and PC rats were treatedwith 1mg/ml L-NAME, NO inhibitor, in drinking water (PLS, PLC) from 24 h beforeip injection to 4 days post-injection (dpi). Rats were individually housed,checked for water and food intake, and weighted every 24 h until 30 dpi. At 4dpi, blood and 24 h urine samples were collected to determine urinary flow andfree water clearance (CH20). Then, rats were euthanized and kidneyswere removed for histopathological observations. NPS and PS rats showed adecrease in food intake and weight with respect to controls (p<0.05). PSrats increased food intake and recovered weight at 5 dpi, while NPS rats showedan improvement at 14 dpi. The water intake increased in NPS and PS ratscompared to controls until 7 dpi (p<0.05). In NPS at 4 dpi, the rise inwater intake coincided with an increase in urinary flow and CH20respect to NPC (p<0.05), different from what was observed in PS. The renalcortex of NPS presented significantly more necrosis and atrophied tubules thanPS (p<0.05). Preliminary results in PLS rats showed that L-NAMEsignificantly increased renal necrosis compared with PLS and PLC rats (p<0.05).In conclusion, PS rats suffered less renal damage and recovered from the Stx2effect faster than NPS rats. L-NAME increased Stx2 effect in PLS suggesting thatphysiology changes caused by pregnancy, like increasing in NO production, may contributeto protect maternal kidney from Stx2 effects.Fil: Fischer Sigel, Lilian Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Sacerdoti, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Zotta, Elsa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Silberstein, Claudia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaLXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología.ArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaFundación Revista Medicina2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195038Effects of Shiga toxin 2 in pregnant and non-pregnant female rats; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología.; Argentina; 2020; 100-1000025-7680CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reunion-anualNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:21:37Zoai:ri.conicet.gov.ar:11336/195038instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:21:37.625CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of Shiga toxin 2 in pregnant and non-pregnant female rats
title Effects of Shiga toxin 2 in pregnant and non-pregnant female rats
spellingShingle Effects of Shiga toxin 2 in pregnant and non-pregnant female rats
Fischer Sigel, Lilian Karina
shiga toxin
pregnant rats
non-pregnant rats
L-NAME
title_short Effects of Shiga toxin 2 in pregnant and non-pregnant female rats
title_full Effects of Shiga toxin 2 in pregnant and non-pregnant female rats
title_fullStr Effects of Shiga toxin 2 in pregnant and non-pregnant female rats
title_full_unstemmed Effects of Shiga toxin 2 in pregnant and non-pregnant female rats
title_sort Effects of Shiga toxin 2 in pregnant and non-pregnant female rats
dc.creator.none.fl_str_mv Fischer Sigel, Lilian Karina
Sacerdoti, Flavia
Ibarra, Cristina Adriana
Zotta, Elsa
Silberstein, Claudia Marcela
author Fischer Sigel, Lilian Karina
author_facet Fischer Sigel, Lilian Karina
Sacerdoti, Flavia
Ibarra, Cristina Adriana
Zotta, Elsa
Silberstein, Claudia Marcela
author_role author
author2 Sacerdoti, Flavia
Ibarra, Cristina Adriana
Zotta, Elsa
Silberstein, Claudia Marcela
author2_role author
author
author
author
dc.subject.none.fl_str_mv shiga toxin
pregnant rats
non-pregnant rats
L-NAME
topic shiga toxin
pregnant rats
non-pregnant rats
L-NAME
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Shigatoxin-producing Escherichia colicauses acute renal failure and Hemolytic Uremic Syndrome. It was reported that inhibition of nitric oxide(NO) by Shiga toxin type 2 (Stx2) enhanced renal damage in mice and baboonmodels of Stx-mediated HUS. The aim of the work was to study the evolution ofthe damages caused by Stx2 in P compare with NP rats. Pregnant Sprague-Dawleyrats, at day 8 of gestation, and NP rats were ip inoculated with 0.5 ng Stx2/gbody weight (PS, NPS) or diluent (PC, NPC). Some PS and PC rats were treatedwith 1mg/ml L-NAME, NO inhibitor, in drinking water (PLS, PLC) from 24 h beforeip injection to 4 days post-injection (dpi). Rats were individually housed,checked for water and food intake, and weighted every 24 h until 30 dpi. At 4dpi, blood and 24 h urine samples were collected to determine urinary flow andfree water clearance (CH20). Then, rats were euthanized and kidneyswere removed for histopathological observations. NPS and PS rats showed adecrease in food intake and weight with respect to controls (p<0.05). PSrats increased food intake and recovered weight at 5 dpi, while NPS rats showedan improvement at 14 dpi. The water intake increased in NPS and PS ratscompared to controls until 7 dpi (p<0.05). In NPS at 4 dpi, the rise inwater intake coincided with an increase in urinary flow and CH20respect to NPC (p<0.05), different from what was observed in PS. The renalcortex of NPS presented significantly more necrosis and atrophied tubules thanPS (p<0.05). Preliminary results in PLS rats showed that L-NAMEsignificantly increased renal necrosis compared with PLS and PLC rats (p<0.05).In conclusion, PS rats suffered less renal damage and recovered from the Stx2effect faster than NPS rats. L-NAME increased Stx2 effect in PLS suggesting thatphysiology changes caused by pregnancy, like increasing in NO production, may contributeto protect maternal kidney from Stx2 effects.
Fil: Fischer Sigel, Lilian Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Sacerdoti, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Zotta, Elsa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Silberstein, Claudia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología.
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
description Shigatoxin-producing Escherichia colicauses acute renal failure and Hemolytic Uremic Syndrome. It was reported that inhibition of nitric oxide(NO) by Shiga toxin type 2 (Stx2) enhanced renal damage in mice and baboonmodels of Stx-mediated HUS. The aim of the work was to study the evolution ofthe damages caused by Stx2 in P compare with NP rats. Pregnant Sprague-Dawleyrats, at day 8 of gestation, and NP rats were ip inoculated with 0.5 ng Stx2/gbody weight (PS, NPS) or diluent (PC, NPC). Some PS and PC rats were treatedwith 1mg/ml L-NAME, NO inhibitor, in drinking water (PLS, PLC) from 24 h beforeip injection to 4 days post-injection (dpi). Rats were individually housed,checked for water and food intake, and weighted every 24 h until 30 dpi. At 4dpi, blood and 24 h urine samples were collected to determine urinary flow andfree water clearance (CH20). Then, rats were euthanized and kidneyswere removed for histopathological observations. NPS and PS rats showed adecrease in food intake and weight with respect to controls (p<0.05). PSrats increased food intake and recovered weight at 5 dpi, while NPS rats showedan improvement at 14 dpi. The water intake increased in NPS and PS ratscompared to controls until 7 dpi (p<0.05). In NPS at 4 dpi, the rise inwater intake coincided with an increase in urinary flow and CH20respect to NPC (p<0.05), different from what was observed in PS. The renalcortex of NPS presented significantly more necrosis and atrophied tubules thanPS (p<0.05). Preliminary results in PLS rats showed that L-NAMEsignificantly increased renal necrosis compared with PLS and PLC rats (p<0.05).In conclusion, PS rats suffered less renal damage and recovered from the Stx2effect faster than NPS rats. L-NAME increased Stx2 effect in PLS suggesting thatphysiology changes caused by pregnancy, like increasing in NO production, may contributeto protect maternal kidney from Stx2 effects.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195038
Effects of Shiga toxin 2 in pregnant and non-pregnant female rats; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología.; Argentina; 2020; 100-100
0025-7680
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195038
identifier_str_mv Effects of Shiga toxin 2 in pregnant and non-pregnant female rats; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología.; Argentina; 2020; 100-100
0025-7680
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reunion-anual
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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