Andrimid production at low temperature by a psychrotolerant Serratia proteamaculans strain

Autores
Sanchez, Leandro Arturo; González Sierra, Manuel; Siñeriz, Faustino; Delgado, Osvaldo Daniel
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Andrimid, a known non-ribosomal pseudo- peptide antibiotic, was isolated from a psychrotolerant Serratia proteamaculans strain. The antibiotic peptide was produced at low temperature (8 °C) in a 7.5 l BIOFLO 101 bioreactor under batch culture mode. Andrimid activity from S. proteamaculans culture was only detected at 25 °C and below and potent antibacterial activity was revealed against both, pathogenic and non-pathogenic bacteria. Minimal inhibitory concentration values determined by microdilution experiments varied in the range between 0.01 and 0.78 lg/ml. Antimicrobial purification and structure elucidation were carried out by LC-MS/MS and 1H/13C NMR approaches. The effects on the ultrastructure of sensitive Escherichia coli 35,218 cells were observed by transmission electron microscopy at different inhibition stages. This work demonstrated the significance of biop- rospection from cold environments through the screening of microorganisms with ability to produce cold-active biomolecules of biotechnological interest. S. proteamacu- lans 136 was revealed as a novel microbial source for andrimid production at low temperatures, showing bio- technological potential to be applied in cryopreservation, food or cosmetic industries against pathogenic bacteria.
Fil: Sanchez, Leandro Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: González Sierra, Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina
Fil: Siñeriz, Faustino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Materia
ANDRIMID
ANTIMICROBIAL
PSEUDO-PEPTIDE
PSYCHROTOLERANT
SERRATIA PROTEAMACULANS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1790
Acceder
id CONICETDig_f6b3e755894f464ec94af557dea69a47
oai_identifier_str oai:ri.conicet.gov.ar:11336/1790
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Andrimid production at low temperature by a psychrotolerant Serratia proteamaculans strainSanchez, Leandro ArturoGonzález Sierra, ManuelSiñeriz, FaustinoDelgado, Osvaldo DanielANDRIMIDANTIMICROBIALPSEUDO-PEPTIDEPSYCHROTOLERANTSERRATIA PROTEAMACULANShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Andrimid, a known non-ribosomal pseudo- peptide antibiotic, was isolated from a psychrotolerant Serratia proteamaculans strain. The antibiotic peptide was produced at low temperature (8 °C) in a 7.5 l BIOFLO 101 bioreactor under batch culture mode. Andrimid activity from S. proteamaculans culture was only detected at 25 °C and below and potent antibacterial activity was revealed against both, pathogenic and non-pathogenic bacteria. Minimal inhibitory concentration values determined by microdilution experiments varied in the range between 0.01 and 0.78 lg/ml. Antimicrobial purification and structure elucidation were carried out by LC-MS/MS and 1H/13C NMR approaches. The effects on the ultrastructure of sensitive Escherichia coli 35,218 cells were observed by transmission electron microscopy at different inhibition stages. This work demonstrated the significance of biop- rospection from cold environments through the screening of microorganisms with ability to produce cold-active biomolecules of biotechnological interest. S. proteamacu- lans 136 was revealed as a novel microbial source for andrimid production at low temperatures, showing bio- technological potential to be applied in cryopreservation, food or cosmetic industries against pathogenic bacteria.Fil: Sanchez, Leandro Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: González Sierra, Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; ArgentinaFil: Siñeriz, Faustino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaSpringer2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1790Sanchez, Leandro Arturo; González Sierra, Manuel; Siñeriz, Faustino; Delgado, Osvaldo Daniel; Andrimid production at low temperature by a psychrotolerant Serratia proteamaculans strain; Springer; World Journal of Microbiology; 29; 10; 4-2013; 1773-17810959-3993enginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11274-013-1338-9info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s11274-013-1338-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:04:20Zoai:ri.conicet.gov.ar:11336/1790instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:04:20.554CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Andrimid production at low temperature by a psychrotolerant Serratia proteamaculans strain
title Andrimid production at low temperature by a psychrotolerant Serratia proteamaculans strain
spellingShingle Andrimid production at low temperature by a psychrotolerant Serratia proteamaculans strain
Sanchez, Leandro Arturo
ANDRIMID
ANTIMICROBIAL
PSEUDO-PEPTIDE
PSYCHROTOLERANT
SERRATIA PROTEAMACULANS
title_short Andrimid production at low temperature by a psychrotolerant Serratia proteamaculans strain
title_full Andrimid production at low temperature by a psychrotolerant Serratia proteamaculans strain
title_fullStr Andrimid production at low temperature by a psychrotolerant Serratia proteamaculans strain
title_full_unstemmed Andrimid production at low temperature by a psychrotolerant Serratia proteamaculans strain
title_sort Andrimid production at low temperature by a psychrotolerant Serratia proteamaculans strain
dc.creator.none.fl_str_mv Sanchez, Leandro Arturo
González Sierra, Manuel
Siñeriz, Faustino
Delgado, Osvaldo Daniel
author Sanchez, Leandro Arturo
author_facet Sanchez, Leandro Arturo
González Sierra, Manuel
Siñeriz, Faustino
Delgado, Osvaldo Daniel
author_role author
author2 González Sierra, Manuel
Siñeriz, Faustino
Delgado, Osvaldo Daniel
author2_role author
author
author
dc.subject.none.fl_str_mv ANDRIMID
ANTIMICROBIAL
PSEUDO-PEPTIDE
PSYCHROTOLERANT
SERRATIA PROTEAMACULANS
topic ANDRIMID
ANTIMICROBIAL
PSEUDO-PEPTIDE
PSYCHROTOLERANT
SERRATIA PROTEAMACULANS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Andrimid, a known non-ribosomal pseudo- peptide antibiotic, was isolated from a psychrotolerant Serratia proteamaculans strain. The antibiotic peptide was produced at low temperature (8 °C) in a 7.5 l BIOFLO 101 bioreactor under batch culture mode. Andrimid activity from S. proteamaculans culture was only detected at 25 °C and below and potent antibacterial activity was revealed against both, pathogenic and non-pathogenic bacteria. Minimal inhibitory concentration values determined by microdilution experiments varied in the range between 0.01 and 0.78 lg/ml. Antimicrobial purification and structure elucidation were carried out by LC-MS/MS and 1H/13C NMR approaches. The effects on the ultrastructure of sensitive Escherichia coli 35,218 cells were observed by transmission electron microscopy at different inhibition stages. This work demonstrated the significance of biop- rospection from cold environments through the screening of microorganisms with ability to produce cold-active biomolecules of biotechnological interest. S. proteamacu- lans 136 was revealed as a novel microbial source for andrimid production at low temperatures, showing bio- technological potential to be applied in cryopreservation, food or cosmetic industries against pathogenic bacteria.
Fil: Sanchez, Leandro Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: González Sierra, Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina
Fil: Siñeriz, Faustino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
description Andrimid, a known non-ribosomal pseudo- peptide antibiotic, was isolated from a psychrotolerant Serratia proteamaculans strain. The antibiotic peptide was produced at low temperature (8 °C) in a 7.5 l BIOFLO 101 bioreactor under batch culture mode. Andrimid activity from S. proteamaculans culture was only detected at 25 °C and below and potent antibacterial activity was revealed against both, pathogenic and non-pathogenic bacteria. Minimal inhibitory concentration values determined by microdilution experiments varied in the range between 0.01 and 0.78 lg/ml. Antimicrobial purification and structure elucidation were carried out by LC-MS/MS and 1H/13C NMR approaches. The effects on the ultrastructure of sensitive Escherichia coli 35,218 cells were observed by transmission electron microscopy at different inhibition stages. This work demonstrated the significance of biop- rospection from cold environments through the screening of microorganisms with ability to produce cold-active biomolecules of biotechnological interest. S. proteamacu- lans 136 was revealed as a novel microbial source for andrimid production at low temperatures, showing bio- technological potential to be applied in cryopreservation, food or cosmetic industries against pathogenic bacteria.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1790
Sanchez, Leandro Arturo; González Sierra, Manuel; Siñeriz, Faustino; Delgado, Osvaldo Daniel; Andrimid production at low temperature by a psychrotolerant Serratia proteamaculans strain; Springer; World Journal of Microbiology; 29; 10; 4-2013; 1773-1781
0959-3993
url http://hdl.handle.net/11336/1790
identifier_str_mv Sanchez, Leandro Arturo; González Sierra, Manuel; Siñeriz, Faustino; Delgado, Osvaldo Daniel; Andrimid production at low temperature by a psychrotolerant Serratia proteamaculans strain; Springer; World Journal of Microbiology; 29; 10; 4-2013; 1773-1781
0959-3993
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s11274-013-1338-9
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s11274-013-1338-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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