Cold-active α-l-rhamnosidase from psychrotolerant bacteria isolated from a sub-Antarctic ecosystem

Autores
Orrillo, Alfredo Gastón; Ledesma, Pablo; Delgado, Osvaldo Daniel; Spagna, Giovanni; Breccia, Javier Dario
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Psychrotolerant bacteria obtained from sea water and alimentary tracts of benthonic organisms from the sub-Antarctic environment of Tierra del Fuego (Argentina) were screened for cold-active A-l-rhamnosidase production. Only 10 of a total of 140 isolates were positive for A-l-rhamnosidase activity as determined by a qualitative assay; and five of them showed substantial activity at 5 °C. Molecular identification (16S rDNA) of five selected isolates revealed that four of them were closely related to the genus Pseudoalteromonas while the other was identified as Ralstonia pickettii. The Pseudoalteromonas sp. 005NJ strain was selected for further characterization based on the higher relative A-l-rhamnosidase activity at 4 °C. It showed specific growth rates of 0.12 to 0.19 1/h in the temperature range of 1 to 8 °C, and the A-l-rhamnosidase activity was mainly intracellular. The reaction showed optimal pH and temperature values of 6 and 40 °C respectively and maintained 6% activity at 4 °C. The enzyme was found to be thermo-sensitive, presenting a half-live of 4 min at 50 °C. In presence of 12% (v/v) ethanol the activity decreased by approximately 48%. Cold-active A-l-rhamnosidase might be useful for food processing technologies.
Fil: Orrillo, Alfredo Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ledesma, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Spagna, Giovanni. Universitá di Catania; Italia
Fil: Breccia, Javier Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Materia
Psychrotolerant
Α-L-Rhamnosidase
Pseudoalteromonas
Ralstonia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/45864
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/45864
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cold-active α-l-rhamnosidase from psychrotolerant bacteria isolated from a sub-Antarctic ecosystemOrrillo, Alfredo GastónLedesma, PabloDelgado, Osvaldo DanielSpagna, GiovanniBreccia, Javier DarioPsychrotolerantΑ-L-RhamnosidasePseudoalteromonasRalstoniahttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Psychrotolerant bacteria obtained from sea water and alimentary tracts of benthonic organisms from the sub-Antarctic environment of Tierra del Fuego (Argentina) were screened for cold-active A-l-rhamnosidase production. Only 10 of a total of 140 isolates were positive for A-l-rhamnosidase activity as determined by a qualitative assay; and five of them showed substantial activity at 5 °C. Molecular identification (16S rDNA) of five selected isolates revealed that four of them were closely related to the genus Pseudoalteromonas while the other was identified as Ralstonia pickettii. The Pseudoalteromonas sp. 005NJ strain was selected for further characterization based on the higher relative A-l-rhamnosidase activity at 4 °C. It showed specific growth rates of 0.12 to 0.19 1/h in the temperature range of 1 to 8 °C, and the A-l-rhamnosidase activity was mainly intracellular. The reaction showed optimal pH and temperature values of 6 and 40 °C respectively and maintained 6% activity at 4 °C. The enzyme was found to be thermo-sensitive, presenting a half-live of 4 min at 50 °C. In presence of 12% (v/v) ethanol the activity decreased by approximately 48%. Cold-active A-l-rhamnosidase might be useful for food processing technologies.Fil: Orrillo, Alfredo Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Ledesma, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Spagna, Giovanni. Universitá di Catania; ItaliaFil: Breccia, Javier Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; ArgentinaElsevier Science Inc2007-01-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/45864Orrillo, Alfredo Gastón; Ledesma, Pablo; Delgado, Osvaldo Daniel; Spagna, Giovanni; Breccia, Javier Dario; Cold-active α-l-rhamnosidase from psychrotolerant bacteria isolated from a sub-Antarctic ecosystem; Elsevier Science Inc; Enzyme and Microbial Technology; 40; 2; 4-1-2007; 236-2410141-0229CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.enzmictec.2006.04.002info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0141022906002006info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:11Zoai:ri.conicet.gov.ar:11336/45864instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:11.757CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cold-active α-l-rhamnosidase from psychrotolerant bacteria isolated from a sub-Antarctic ecosystem
title Cold-active α-l-rhamnosidase from psychrotolerant bacteria isolated from a sub-Antarctic ecosystem
spellingShingle Cold-active α-l-rhamnosidase from psychrotolerant bacteria isolated from a sub-Antarctic ecosystem
Orrillo, Alfredo Gastón
Psychrotolerant
Α-L-Rhamnosidase
Pseudoalteromonas
Ralstonia
title_short Cold-active α-l-rhamnosidase from psychrotolerant bacteria isolated from a sub-Antarctic ecosystem
title_full Cold-active α-l-rhamnosidase from psychrotolerant bacteria isolated from a sub-Antarctic ecosystem
title_fullStr Cold-active α-l-rhamnosidase from psychrotolerant bacteria isolated from a sub-Antarctic ecosystem
title_full_unstemmed Cold-active α-l-rhamnosidase from psychrotolerant bacteria isolated from a sub-Antarctic ecosystem
title_sort Cold-active α-l-rhamnosidase from psychrotolerant bacteria isolated from a sub-Antarctic ecosystem
dc.creator.none.fl_str_mv Orrillo, Alfredo Gastón
Ledesma, Pablo
Delgado, Osvaldo Daniel
Spagna, Giovanni
Breccia, Javier Dario
author Orrillo, Alfredo Gastón
author_facet Orrillo, Alfredo Gastón
Ledesma, Pablo
Delgado, Osvaldo Daniel
Spagna, Giovanni
Breccia, Javier Dario
author_role author
author2 Ledesma, Pablo
Delgado, Osvaldo Daniel
Spagna, Giovanni
Breccia, Javier Dario
author2_role author
author
author
author
dc.subject.none.fl_str_mv Psychrotolerant
Α-L-Rhamnosidase
Pseudoalteromonas
Ralstonia
topic Psychrotolerant
Α-L-Rhamnosidase
Pseudoalteromonas
Ralstonia
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Psychrotolerant bacteria obtained from sea water and alimentary tracts of benthonic organisms from the sub-Antarctic environment of Tierra del Fuego (Argentina) were screened for cold-active A-l-rhamnosidase production. Only 10 of a total of 140 isolates were positive for A-l-rhamnosidase activity as determined by a qualitative assay; and five of them showed substantial activity at 5 °C. Molecular identification (16S rDNA) of five selected isolates revealed that four of them were closely related to the genus Pseudoalteromonas while the other was identified as Ralstonia pickettii. The Pseudoalteromonas sp. 005NJ strain was selected for further characterization based on the higher relative A-l-rhamnosidase activity at 4 °C. It showed specific growth rates of 0.12 to 0.19 1/h in the temperature range of 1 to 8 °C, and the A-l-rhamnosidase activity was mainly intracellular. The reaction showed optimal pH and temperature values of 6 and 40 °C respectively and maintained 6% activity at 4 °C. The enzyme was found to be thermo-sensitive, presenting a half-live of 4 min at 50 °C. In presence of 12% (v/v) ethanol the activity decreased by approximately 48%. Cold-active A-l-rhamnosidase might be useful for food processing technologies.
Fil: Orrillo, Alfredo Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ledesma, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Spagna, Giovanni. Universitá di Catania; Italia
Fil: Breccia, Javier Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
description Psychrotolerant bacteria obtained from sea water and alimentary tracts of benthonic organisms from the sub-Antarctic environment of Tierra del Fuego (Argentina) were screened for cold-active A-l-rhamnosidase production. Only 10 of a total of 140 isolates were positive for A-l-rhamnosidase activity as determined by a qualitative assay; and five of them showed substantial activity at 5 °C. Molecular identification (16S rDNA) of five selected isolates revealed that four of them were closely related to the genus Pseudoalteromonas while the other was identified as Ralstonia pickettii. The Pseudoalteromonas sp. 005NJ strain was selected for further characterization based on the higher relative A-l-rhamnosidase activity at 4 °C. It showed specific growth rates of 0.12 to 0.19 1/h in the temperature range of 1 to 8 °C, and the A-l-rhamnosidase activity was mainly intracellular. The reaction showed optimal pH and temperature values of 6 and 40 °C respectively and maintained 6% activity at 4 °C. The enzyme was found to be thermo-sensitive, presenting a half-live of 4 min at 50 °C. In presence of 12% (v/v) ethanol the activity decreased by approximately 48%. Cold-active A-l-rhamnosidase might be useful for food processing technologies.
publishDate 2007
dc.date.none.fl_str_mv 2007-01-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/45864
Orrillo, Alfredo Gastón; Ledesma, Pablo; Delgado, Osvaldo Daniel; Spagna, Giovanni; Breccia, Javier Dario; Cold-active α-l-rhamnosidase from psychrotolerant bacteria isolated from a sub-Antarctic ecosystem; Elsevier Science Inc; Enzyme and Microbial Technology; 40; 2; 4-1-2007; 236-241
0141-0229
CONICET Digital
CONICET
url http://hdl.handle.net/11336/45864
identifier_str_mv Orrillo, Alfredo Gastón; Ledesma, Pablo; Delgado, Osvaldo Daniel; Spagna, Giovanni; Breccia, Javier Dario; Cold-active α-l-rhamnosidase from psychrotolerant bacteria isolated from a sub-Antarctic ecosystem; Elsevier Science Inc; Enzyme and Microbial Technology; 40; 2; 4-1-2007; 236-241
0141-0229
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.enzmictec.2006.04.002
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0141022906002006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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