Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia)
- Autores
- Arnau, Victor Gonzalo; Sanchez, Leandro Arturo; Delgado, Osvaldo Daniel
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A psychrotolerant strain, 8H1T was isolated from soil samples collected in Isla de los Estados , Ushuaia, Argentina. Cells were Gram negative, aerobic, straight rods, occurring singly or in pairs, non-spore-forming and motile by mean of two polar flagella. The isolate was able to grow in the 4-35 °C range, with an optimum rate at 28 °C. The predominant cellular fatty acids were summed feature 3 (C16:1 ω6c and/or C16:1 ω7c; 38.0 %), C16:0 (35.4 %) and summed feature 8 (C18:1 ω7c; 9.5 %). The polar lipid pattern of strain 8H1T comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phospholipid. Ubiquinone 9 (Q-9) was the predominant lipoquinone. DNA G+C content was 59.8 mol%. 16S rRNA gene sequence-based phylogeny suggested the affiliation of isolate 8H1T to the Pseudomonas fluorescens group; displaying ≥98.5 % sequence similarities among 29 type strains. However, MLSA study by concatenating 16S rRNA, gyrB, rpoD and rpoB genes showed that isolate 8H1T could be discriminated from closely related Pseudomonas species and placed in the Pseudomonas gessardii subgroup, including the species with the highest MLSA sequence similarities: P. brenneriT (96.2 %), P. gessardiiT (96.1 %), P. proteolyticaT (96.0 %), P. meridianaT (96.0 %), P. mucidolensT (95.4 %). DNA-DNA hybridization analysis between 8H1T and these closely related type strains revealed relatedness values of 27.0, 8.8, 41.2, 39.7 and 46.1 %, respectively. These results together with differences in several phenotypic features support the classification of a novel species for which the name Pseudomonas yamanorum sp. nov. is proposed. The type strain is 8H1T (=DSM 26522T =CCUG 63249T= LMG 27247T).
Fil: Arnau, Victor Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina
Fil: Sanchez, Leandro Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina
Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro de Investigaciones y Transferencia de Catamarca; Argentina. Universidad Nacional de Catamarca; Argentina - Materia
-
Pseudomonas Yamanorum
Tierra del Fuego
Psychrotolerant Bacteria - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/16861
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Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia)Arnau, Victor GonzaloSanchez, Leandro ArturoDelgado, Osvaldo DanielPseudomonas YamanorumTierra del FuegoPsychrotolerant Bacteriahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A psychrotolerant strain, 8H1T was isolated from soil samples collected in Isla de los Estados , Ushuaia, Argentina. Cells were Gram negative, aerobic, straight rods, occurring singly or in pairs, non-spore-forming and motile by mean of two polar flagella. The isolate was able to grow in the 4-35 °C range, with an optimum rate at 28 °C. The predominant cellular fatty acids were summed feature 3 (C16:1 ω6c and/or C16:1 ω7c; 38.0 %), C16:0 (35.4 %) and summed feature 8 (C18:1 ω7c; 9.5 %). The polar lipid pattern of strain 8H1T comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phospholipid. Ubiquinone 9 (Q-9) was the predominant lipoquinone. DNA G+C content was 59.8 mol%. 16S rRNA gene sequence-based phylogeny suggested the affiliation of isolate 8H1T to the Pseudomonas fluorescens group; displaying ≥98.5 % sequence similarities among 29 type strains. However, MLSA study by concatenating 16S rRNA, gyrB, rpoD and rpoB genes showed that isolate 8H1T could be discriminated from closely related Pseudomonas species and placed in the Pseudomonas gessardii subgroup, including the species with the highest MLSA sequence similarities: P. brenneriT (96.2 %), P. gessardiiT (96.1 %), P. proteolyticaT (96.0 %), P. meridianaT (96.0 %), P. mucidolensT (95.4 %). DNA-DNA hybridization analysis between 8H1T and these closely related type strains revealed relatedness values of 27.0, 8.8, 41.2, 39.7 and 46.1 %, respectively. These results together with differences in several phenotypic features support the classification of a novel species for which the name Pseudomonas yamanorum sp. nov. is proposed. The type strain is 8H1T (=DSM 26522T =CCUG 63249T= LMG 27247T).Fil: Arnau, Victor Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); ArgentinaFil: Sanchez, Leandro Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); ArgentinaFil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro de Investigaciones y Transferencia de Catamarca; Argentina. Universidad Nacional de Catamarca; ArgentinaSociety For General Microbiology2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16861Arnau, Victor Gonzalo; Sanchez, Leandro Arturo; Delgado, Osvaldo Daniel; Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia); Society For General Microbiology; International Journal Of Systematic And Evolutionary Microbiology.; 65; 2; 2-2015; 424-4311466-5026enginfo:eu-repo/semantics/altIdentifier/doi/10.1099/ijs.0.065201-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:20:59Zoai:ri.conicet.gov.ar:11336/16861instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:20:59.68CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia) |
title |
Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia) |
spellingShingle |
Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia) Arnau, Victor Gonzalo Pseudomonas Yamanorum Tierra del Fuego Psychrotolerant Bacteria |
title_short |
Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia) |
title_full |
Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia) |
title_fullStr |
Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia) |
title_full_unstemmed |
Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia) |
title_sort |
Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia) |
dc.creator.none.fl_str_mv |
Arnau, Victor Gonzalo Sanchez, Leandro Arturo Delgado, Osvaldo Daniel |
author |
Arnau, Victor Gonzalo |
author_facet |
Arnau, Victor Gonzalo Sanchez, Leandro Arturo Delgado, Osvaldo Daniel |
author_role |
author |
author2 |
Sanchez, Leandro Arturo Delgado, Osvaldo Daniel |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Pseudomonas Yamanorum Tierra del Fuego Psychrotolerant Bacteria |
topic |
Pseudomonas Yamanorum Tierra del Fuego Psychrotolerant Bacteria |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
A psychrotolerant strain, 8H1T was isolated from soil samples collected in Isla de los Estados , Ushuaia, Argentina. Cells were Gram negative, aerobic, straight rods, occurring singly or in pairs, non-spore-forming and motile by mean of two polar flagella. The isolate was able to grow in the 4-35 °C range, with an optimum rate at 28 °C. The predominant cellular fatty acids were summed feature 3 (C16:1 ω6c and/or C16:1 ω7c; 38.0 %), C16:0 (35.4 %) and summed feature 8 (C18:1 ω7c; 9.5 %). The polar lipid pattern of strain 8H1T comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phospholipid. Ubiquinone 9 (Q-9) was the predominant lipoquinone. DNA G+C content was 59.8 mol%. 16S rRNA gene sequence-based phylogeny suggested the affiliation of isolate 8H1T to the Pseudomonas fluorescens group; displaying ≥98.5 % sequence similarities among 29 type strains. However, MLSA study by concatenating 16S rRNA, gyrB, rpoD and rpoB genes showed that isolate 8H1T could be discriminated from closely related Pseudomonas species and placed in the Pseudomonas gessardii subgroup, including the species with the highest MLSA sequence similarities: P. brenneriT (96.2 %), P. gessardiiT (96.1 %), P. proteolyticaT (96.0 %), P. meridianaT (96.0 %), P. mucidolensT (95.4 %). DNA-DNA hybridization analysis between 8H1T and these closely related type strains revealed relatedness values of 27.0, 8.8, 41.2, 39.7 and 46.1 %, respectively. These results together with differences in several phenotypic features support the classification of a novel species for which the name Pseudomonas yamanorum sp. nov. is proposed. The type strain is 8H1T (=DSM 26522T =CCUG 63249T= LMG 27247T). Fil: Arnau, Victor Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina Fil: Sanchez, Leandro Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro de Investigaciones y Transferencia de Catamarca; Argentina. Universidad Nacional de Catamarca; Argentina |
description |
A psychrotolerant strain, 8H1T was isolated from soil samples collected in Isla de los Estados , Ushuaia, Argentina. Cells were Gram negative, aerobic, straight rods, occurring singly or in pairs, non-spore-forming and motile by mean of two polar flagella. The isolate was able to grow in the 4-35 °C range, with an optimum rate at 28 °C. The predominant cellular fatty acids were summed feature 3 (C16:1 ω6c and/or C16:1 ω7c; 38.0 %), C16:0 (35.4 %) and summed feature 8 (C18:1 ω7c; 9.5 %). The polar lipid pattern of strain 8H1T comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phospholipid. Ubiquinone 9 (Q-9) was the predominant lipoquinone. DNA G+C content was 59.8 mol%. 16S rRNA gene sequence-based phylogeny suggested the affiliation of isolate 8H1T to the Pseudomonas fluorescens group; displaying ≥98.5 % sequence similarities among 29 type strains. However, MLSA study by concatenating 16S rRNA, gyrB, rpoD and rpoB genes showed that isolate 8H1T could be discriminated from closely related Pseudomonas species and placed in the Pseudomonas gessardii subgroup, including the species with the highest MLSA sequence similarities: P. brenneriT (96.2 %), P. gessardiiT (96.1 %), P. proteolyticaT (96.0 %), P. meridianaT (96.0 %), P. mucidolensT (95.4 %). DNA-DNA hybridization analysis between 8H1T and these closely related type strains revealed relatedness values of 27.0, 8.8, 41.2, 39.7 and 46.1 %, respectively. These results together with differences in several phenotypic features support the classification of a novel species for which the name Pseudomonas yamanorum sp. nov. is proposed. The type strain is 8H1T (=DSM 26522T =CCUG 63249T= LMG 27247T). |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/16861 Arnau, Victor Gonzalo; Sanchez, Leandro Arturo; Delgado, Osvaldo Daniel; Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia); Society For General Microbiology; International Journal Of Systematic And Evolutionary Microbiology.; 65; 2; 2-2015; 424-431 1466-5026 |
url |
http://hdl.handle.net/11336/16861 |
identifier_str_mv |
Arnau, Victor Gonzalo; Sanchez, Leandro Arturo; Delgado, Osvaldo Daniel; Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia); Society For General Microbiology; International Journal Of Systematic And Evolutionary Microbiology.; 65; 2; 2-2015; 424-431 1466-5026 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1099/ijs.0.065201-0 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Society For General Microbiology |
publisher.none.fl_str_mv |
Society For General Microbiology |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614195387039744 |
score |
13.070432 |