Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia)

Autores
Arnau, Victor Gonzalo; Sanchez, Leandro Arturo; Delgado, Osvaldo Daniel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A psychrotolerant strain, 8H1T was isolated from soil samples collected in Isla de los Estados , Ushuaia, Argentina. Cells were Gram negative, aerobic, straight rods, occurring singly or in pairs, non-spore-forming and motile by mean of two polar flagella. The isolate was able to grow in the 4-35 °C range, with an optimum rate at 28 °C. The predominant cellular fatty acids were summed feature 3 (C16:1 ω6c and/or C16:1 ω7c; 38.0 %), C16:0 (35.4 %) and summed feature 8 (C18:1 ω7c; 9.5 %). The polar lipid pattern of strain 8H1T comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phospholipid. Ubiquinone 9 (Q-9) was the predominant lipoquinone. DNA G+C content was 59.8 mol%. 16S rRNA gene sequence-based phylogeny suggested the affiliation of isolate 8H1T to the Pseudomonas fluorescens group; displaying ≥98.5 % sequence similarities among 29 type strains. However, MLSA study by concatenating 16S rRNA, gyrB, rpoD and rpoB genes showed that isolate 8H1T could be discriminated from closely related Pseudomonas species and placed in the Pseudomonas gessardii subgroup, including the species with the highest MLSA sequence similarities: P. brenneriT (96.2 %), P. gessardiiT (96.1 %), P. proteolyticaT (96.0 %), P. meridianaT (96.0 %), P. mucidolensT (95.4 %). DNA-DNA hybridization analysis between 8H1T and these closely related type strains revealed relatedness values of 27.0, 8.8, 41.2, 39.7 and 46.1 %, respectively. These results together with differences in several phenotypic features support the classification of a novel species for which the name Pseudomonas yamanorum sp. nov. is proposed. The type strain is 8H1T (=DSM 26522T =CCUG 63249T= LMG 27247T).
Fil: Arnau, Victor Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina
Fil: Sanchez, Leandro Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina
Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro de Investigaciones y Transferencia de Catamarca; Argentina. Universidad Nacional de Catamarca; Argentina
Materia
Pseudomonas Yamanorum
Tierra del Fuego
Psychrotolerant Bacteria
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16861
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/16861
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia)Arnau, Victor GonzaloSanchez, Leandro ArturoDelgado, Osvaldo DanielPseudomonas YamanorumTierra del FuegoPsychrotolerant Bacteriahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A psychrotolerant strain, 8H1T was isolated from soil samples collected in Isla de los Estados , Ushuaia, Argentina. Cells were Gram negative, aerobic, straight rods, occurring singly or in pairs, non-spore-forming and motile by mean of two polar flagella. The isolate was able to grow in the 4-35 °C range, with an optimum rate at 28 °C. The predominant cellular fatty acids were summed feature 3 (C16:1 ω6c and/or C16:1 ω7c; 38.0 %), C16:0 (35.4 %) and summed feature 8 (C18:1 ω7c; 9.5 %). The polar lipid pattern of strain 8H1T comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phospholipid. Ubiquinone 9 (Q-9) was the predominant lipoquinone. DNA G+C content was 59.8 mol%. 16S rRNA gene sequence-based phylogeny suggested the affiliation of isolate 8H1T to the Pseudomonas fluorescens group; displaying ≥98.5 % sequence similarities among 29 type strains. However, MLSA study by concatenating 16S rRNA, gyrB, rpoD and rpoB genes showed that isolate 8H1T could be discriminated from closely related Pseudomonas species and placed in the Pseudomonas gessardii subgroup, including the species with the highest MLSA sequence similarities: P. brenneriT (96.2 %), P. gessardiiT (96.1 %), P. proteolyticaT (96.0 %), P. meridianaT (96.0 %), P. mucidolensT (95.4 %). DNA-DNA hybridization analysis between 8H1T and these closely related type strains revealed relatedness values of 27.0, 8.8, 41.2, 39.7 and 46.1 %, respectively. These results together with differences in several phenotypic features support the classification of a novel species for which the name Pseudomonas yamanorum sp. nov. is proposed. The type strain is 8H1T (=DSM 26522T =CCUG 63249T= LMG 27247T).Fil: Arnau, Victor Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); ArgentinaFil: Sanchez, Leandro Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); ArgentinaFil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro de Investigaciones y Transferencia de Catamarca; Argentina. Universidad Nacional de Catamarca; ArgentinaSociety For General Microbiology2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16861Arnau, Victor Gonzalo; Sanchez, Leandro Arturo; Delgado, Osvaldo Daniel; Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia); Society For General Microbiology; International Journal Of Systematic And Evolutionary Microbiology.; 65; 2; 2-2015; 424-4311466-5026enginfo:eu-repo/semantics/altIdentifier/doi/10.1099/ijs.0.065201-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:53:40Zoai:ri.conicet.gov.ar:11336/16861instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:53:41.269CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia)
title Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia)
spellingShingle Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia)
Arnau, Victor Gonzalo
Pseudomonas Yamanorum
Tierra del Fuego
Psychrotolerant Bacteria
title_short Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia)
title_full Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia)
title_fullStr Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia)
title_full_unstemmed Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia)
title_sort Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia)
dc.creator.none.fl_str_mv Arnau, Victor Gonzalo
Sanchez, Leandro Arturo
Delgado, Osvaldo Daniel
author Arnau, Victor Gonzalo
author_facet Arnau, Victor Gonzalo
Sanchez, Leandro Arturo
Delgado, Osvaldo Daniel
author_role author
author2 Sanchez, Leandro Arturo
Delgado, Osvaldo Daniel
author2_role author
author
dc.subject.none.fl_str_mv Pseudomonas Yamanorum
Tierra del Fuego
Psychrotolerant Bacteria
topic Pseudomonas Yamanorum
Tierra del Fuego
Psychrotolerant Bacteria
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A psychrotolerant strain, 8H1T was isolated from soil samples collected in Isla de los Estados , Ushuaia, Argentina. Cells were Gram negative, aerobic, straight rods, occurring singly or in pairs, non-spore-forming and motile by mean of two polar flagella. The isolate was able to grow in the 4-35 °C range, with an optimum rate at 28 °C. The predominant cellular fatty acids were summed feature 3 (C16:1 ω6c and/or C16:1 ω7c; 38.0 %), C16:0 (35.4 %) and summed feature 8 (C18:1 ω7c; 9.5 %). The polar lipid pattern of strain 8H1T comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phospholipid. Ubiquinone 9 (Q-9) was the predominant lipoquinone. DNA G+C content was 59.8 mol%. 16S rRNA gene sequence-based phylogeny suggested the affiliation of isolate 8H1T to the Pseudomonas fluorescens group; displaying ≥98.5 % sequence similarities among 29 type strains. However, MLSA study by concatenating 16S rRNA, gyrB, rpoD and rpoB genes showed that isolate 8H1T could be discriminated from closely related Pseudomonas species and placed in the Pseudomonas gessardii subgroup, including the species with the highest MLSA sequence similarities: P. brenneriT (96.2 %), P. gessardiiT (96.1 %), P. proteolyticaT (96.0 %), P. meridianaT (96.0 %), P. mucidolensT (95.4 %). DNA-DNA hybridization analysis between 8H1T and these closely related type strains revealed relatedness values of 27.0, 8.8, 41.2, 39.7 and 46.1 %, respectively. These results together with differences in several phenotypic features support the classification of a novel species for which the name Pseudomonas yamanorum sp. nov. is proposed. The type strain is 8H1T (=DSM 26522T =CCUG 63249T= LMG 27247T).
Fil: Arnau, Victor Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina
Fil: Sanchez, Leandro Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina
Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro de Investigaciones y Transferencia de Catamarca; Argentina. Universidad Nacional de Catamarca; Argentina
description A psychrotolerant strain, 8H1T was isolated from soil samples collected in Isla de los Estados , Ushuaia, Argentina. Cells were Gram negative, aerobic, straight rods, occurring singly or in pairs, non-spore-forming and motile by mean of two polar flagella. The isolate was able to grow in the 4-35 °C range, with an optimum rate at 28 °C. The predominant cellular fatty acids were summed feature 3 (C16:1 ω6c and/or C16:1 ω7c; 38.0 %), C16:0 (35.4 %) and summed feature 8 (C18:1 ω7c; 9.5 %). The polar lipid pattern of strain 8H1T comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phospholipid. Ubiquinone 9 (Q-9) was the predominant lipoquinone. DNA G+C content was 59.8 mol%. 16S rRNA gene sequence-based phylogeny suggested the affiliation of isolate 8H1T to the Pseudomonas fluorescens group; displaying ≥98.5 % sequence similarities among 29 type strains. However, MLSA study by concatenating 16S rRNA, gyrB, rpoD and rpoB genes showed that isolate 8H1T could be discriminated from closely related Pseudomonas species and placed in the Pseudomonas gessardii subgroup, including the species with the highest MLSA sequence similarities: P. brenneriT (96.2 %), P. gessardiiT (96.1 %), P. proteolyticaT (96.0 %), P. meridianaT (96.0 %), P. mucidolensT (95.4 %). DNA-DNA hybridization analysis between 8H1T and these closely related type strains revealed relatedness values of 27.0, 8.8, 41.2, 39.7 and 46.1 %, respectively. These results together with differences in several phenotypic features support the classification of a novel species for which the name Pseudomonas yamanorum sp. nov. is proposed. The type strain is 8H1T (=DSM 26522T =CCUG 63249T= LMG 27247T).
publishDate 2015
dc.date.none.fl_str_mv 2015-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16861
Arnau, Victor Gonzalo; Sanchez, Leandro Arturo; Delgado, Osvaldo Daniel; Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia); Society For General Microbiology; International Journal Of Systematic And Evolutionary Microbiology.; 65; 2; 2-2015; 424-431
1466-5026
url http://hdl.handle.net/11336/16861
identifier_str_mv Arnau, Victor Gonzalo; Sanchez, Leandro Arturo; Delgado, Osvaldo Daniel; Pseudomonas yamanorum sp. nov., a new psychrotolerant bacterium isolated from Sub-Antarctic environment (Tierra del Fuego, Ushuaia); Society For General Microbiology; International Journal Of Systematic And Evolutionary Microbiology.; 65; 2; 2-2015; 424-431
1466-5026
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1099/ijs.0.065201-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society For General Microbiology
publisher.none.fl_str_mv Society For General Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.928904

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