Biological activities of essential oils on Caenorhabditis elegans: from molecular target to anthelmintic therapeutic strategies

Autores
Hernando, Guillermina Silvana; Turani, Ornella; Rodriguez Araujo, Noelia; Bouzat, Cecilia Beatriz
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Plants, herbal preparations and essential oils are used for health and medical functions since ancient times. Essential oils (EOs) have been extensively applied for human and veterinary health; their active agents have been isolated and incorporated into many current pharmaceutical preparations. Our study involves the use of Caenorhabditis elegans as a model of parasitic nematodes due to its simplicity and easy maintenance in the laboratory. We start from the hypothesis that EOs of aromatic plants used for aromatherapy or as food additives have anthelmintic action. We developed behavioral and molecular assays in wild-type worms, and analyzed mutant strains lacking receptors involved in locomotion to identify the pharmacological targets mediating the anthelmintic activities. We also explored the combination of current anthelmintics together with the addition of EOs purified compounds as a strategy to reduce resistance. In paralysis assays on agar plates, all tested EOs inhibited with different potencies C. elegans locomotion as well as egg hatching. The major compounds present in the tested EOs, transcinnamaldehyde (TC), geraniol, citronellol and linalool, were active at C. elegans. The combination of TC with the commercial anthelmintics levamisole and monepantel showed synergistic paralysis effects, while its combination with piperazine or ivermectin produced antagonic effects. Mutant worms lacking the levamisole-sensitive nicotinic receptor (L-AChR), GABA and glutamate receptors were partially resistant to these compounds. By single-channel recordings from C. elegans muscle cultured cells we deciphered how L-AChRs are modulated by TC. The analysis revealed that TC acts as an allosteric inhibitor of L-AChRs with key roles in nematode locomotion. Overall, we identified essential oils and their components as novel anthelmintic drugs and revealed their main pharmacological targets. Our results propose EOs as sources of natural compounds with promising polypharmacological profiles for anthelmintic therapeutics, and provide data on the efficacies of combinations that emerge as strategies to reduce drug resistance in nematodes
Fil: Hernando, Guillermina Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Turani, Ornella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Rodriguez Araujo, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
24th International C. elegans Conference
Glasgow
Escocia
Genetics Society of America
Materia
CAENORHABDITIS ELEGANS
ESSENTIAL OILS
ANTHELMINTICS
CYS-LOOP RECEPTORS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/224927
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biological activities of essential oils on Caenorhabditis elegans: from molecular target to anthelmintic therapeutic strategiesHernando, Guillermina SilvanaTurani, OrnellaRodriguez Araujo, NoeliaBouzat, Cecilia BeatrizCAENORHABDITIS ELEGANSESSENTIAL OILSANTHELMINTICSCYS-LOOP RECEPTORShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Plants, herbal preparations and essential oils are used for health and medical functions since ancient times. Essential oils (EOs) have been extensively applied for human and veterinary health; their active agents have been isolated and incorporated into many current pharmaceutical preparations. Our study involves the use of Caenorhabditis elegans as a model of parasitic nematodes due to its simplicity and easy maintenance in the laboratory. We start from the hypothesis that EOs of aromatic plants used for aromatherapy or as food additives have anthelmintic action. We developed behavioral and molecular assays in wild-type worms, and analyzed mutant strains lacking receptors involved in locomotion to identify the pharmacological targets mediating the anthelmintic activities. We also explored the combination of current anthelmintics together with the addition of EOs purified compounds as a strategy to reduce resistance. In paralysis assays on agar plates, all tested EOs inhibited with different potencies C. elegans locomotion as well as egg hatching. The major compounds present in the tested EOs, transcinnamaldehyde (TC), geraniol, citronellol and linalool, were active at C. elegans. The combination of TC with the commercial anthelmintics levamisole and monepantel showed synergistic paralysis effects, while its combination with piperazine or ivermectin produced antagonic effects. Mutant worms lacking the levamisole-sensitive nicotinic receptor (L-AChR), GABA and glutamate receptors were partially resistant to these compounds. By single-channel recordings from C. elegans muscle cultured cells we deciphered how L-AChRs are modulated by TC. The analysis revealed that TC acts as an allosteric inhibitor of L-AChRs with key roles in nematode locomotion. Overall, we identified essential oils and their components as novel anthelmintic drugs and revealed their main pharmacological targets. Our results propose EOs as sources of natural compounds with promising polypharmacological profiles for anthelmintic therapeutics, and provide data on the efficacies of combinations that emerge as strategies to reduce drug resistance in nematodesFil: Hernando, Guillermina Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Turani, Ornella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Rodriguez Araujo, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina24th International C. elegans ConferenceGlasgowEscociaGenetics Society of AmericaGenetics Society of America2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/224927Biological activities of essential oils on Caenorhabditis elegans: from molecular target to anthelmintic therapeutic strategies; 24th International C. elegans Conference; Glasgow; Escocia; 2023; 471-471CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://genetics-gsa.org/celegans2023/program-and-abstract-books/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:42:54Zoai:ri.conicet.gov.ar:11336/224927instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:42:54.503CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biological activities of essential oils on Caenorhabditis elegans: from molecular target to anthelmintic therapeutic strategies
title Biological activities of essential oils on Caenorhabditis elegans: from molecular target to anthelmintic therapeutic strategies
spellingShingle Biological activities of essential oils on Caenorhabditis elegans: from molecular target to anthelmintic therapeutic strategies
Hernando, Guillermina Silvana
CAENORHABDITIS ELEGANS
ESSENTIAL OILS
ANTHELMINTICS
CYS-LOOP RECEPTORS
title_short Biological activities of essential oils on Caenorhabditis elegans: from molecular target to anthelmintic therapeutic strategies
title_full Biological activities of essential oils on Caenorhabditis elegans: from molecular target to anthelmintic therapeutic strategies
title_fullStr Biological activities of essential oils on Caenorhabditis elegans: from molecular target to anthelmintic therapeutic strategies
title_full_unstemmed Biological activities of essential oils on Caenorhabditis elegans: from molecular target to anthelmintic therapeutic strategies
title_sort Biological activities of essential oils on Caenorhabditis elegans: from molecular target to anthelmintic therapeutic strategies
dc.creator.none.fl_str_mv Hernando, Guillermina Silvana
Turani, Ornella
Rodriguez Araujo, Noelia
Bouzat, Cecilia Beatriz
author Hernando, Guillermina Silvana
author_facet Hernando, Guillermina Silvana
Turani, Ornella
Rodriguez Araujo, Noelia
Bouzat, Cecilia Beatriz
author_role author
author2 Turani, Ornella
Rodriguez Araujo, Noelia
Bouzat, Cecilia Beatriz
author2_role author
author
author
dc.subject.none.fl_str_mv CAENORHABDITIS ELEGANS
ESSENTIAL OILS
ANTHELMINTICS
CYS-LOOP RECEPTORS
topic CAENORHABDITIS ELEGANS
ESSENTIAL OILS
ANTHELMINTICS
CYS-LOOP RECEPTORS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Plants, herbal preparations and essential oils are used for health and medical functions since ancient times. Essential oils (EOs) have been extensively applied for human and veterinary health; their active agents have been isolated and incorporated into many current pharmaceutical preparations. Our study involves the use of Caenorhabditis elegans as a model of parasitic nematodes due to its simplicity and easy maintenance in the laboratory. We start from the hypothesis that EOs of aromatic plants used for aromatherapy or as food additives have anthelmintic action. We developed behavioral and molecular assays in wild-type worms, and analyzed mutant strains lacking receptors involved in locomotion to identify the pharmacological targets mediating the anthelmintic activities. We also explored the combination of current anthelmintics together with the addition of EOs purified compounds as a strategy to reduce resistance. In paralysis assays on agar plates, all tested EOs inhibited with different potencies C. elegans locomotion as well as egg hatching. The major compounds present in the tested EOs, transcinnamaldehyde (TC), geraniol, citronellol and linalool, were active at C. elegans. The combination of TC with the commercial anthelmintics levamisole and monepantel showed synergistic paralysis effects, while its combination with piperazine or ivermectin produced antagonic effects. Mutant worms lacking the levamisole-sensitive nicotinic receptor (L-AChR), GABA and glutamate receptors were partially resistant to these compounds. By single-channel recordings from C. elegans muscle cultured cells we deciphered how L-AChRs are modulated by TC. The analysis revealed that TC acts as an allosteric inhibitor of L-AChRs with key roles in nematode locomotion. Overall, we identified essential oils and their components as novel anthelmintic drugs and revealed their main pharmacological targets. Our results propose EOs as sources of natural compounds with promising polypharmacological profiles for anthelmintic therapeutics, and provide data on the efficacies of combinations that emerge as strategies to reduce drug resistance in nematodes
Fil: Hernando, Guillermina Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Turani, Ornella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Rodriguez Araujo, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
24th International C. elegans Conference
Glasgow
Escocia
Genetics Society of America
description Plants, herbal preparations and essential oils are used for health and medical functions since ancient times. Essential oils (EOs) have been extensively applied for human and veterinary health; their active agents have been isolated and incorporated into many current pharmaceutical preparations. Our study involves the use of Caenorhabditis elegans as a model of parasitic nematodes due to its simplicity and easy maintenance in the laboratory. We start from the hypothesis that EOs of aromatic plants used for aromatherapy or as food additives have anthelmintic action. We developed behavioral and molecular assays in wild-type worms, and analyzed mutant strains lacking receptors involved in locomotion to identify the pharmacological targets mediating the anthelmintic activities. We also explored the combination of current anthelmintics together with the addition of EOs purified compounds as a strategy to reduce resistance. In paralysis assays on agar plates, all tested EOs inhibited with different potencies C. elegans locomotion as well as egg hatching. The major compounds present in the tested EOs, transcinnamaldehyde (TC), geraniol, citronellol and linalool, were active at C. elegans. The combination of TC with the commercial anthelmintics levamisole and monepantel showed synergistic paralysis effects, while its combination with piperazine or ivermectin produced antagonic effects. Mutant worms lacking the levamisole-sensitive nicotinic receptor (L-AChR), GABA and glutamate receptors were partially resistant to these compounds. By single-channel recordings from C. elegans muscle cultured cells we deciphered how L-AChRs are modulated by TC. The analysis revealed that TC acts as an allosteric inhibitor of L-AChRs with key roles in nematode locomotion. Overall, we identified essential oils and their components as novel anthelmintic drugs and revealed their main pharmacological targets. Our results propose EOs as sources of natural compounds with promising polypharmacological profiles for anthelmintic therapeutics, and provide data on the efficacies of combinations that emerge as strategies to reduce drug resistance in nematodes
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/224927
Biological activities of essential oils on Caenorhabditis elegans: from molecular target to anthelmintic therapeutic strategies; 24th International C. elegans Conference; Glasgow; Escocia; 2023; 471-471
CONICET Digital
CONICET
url http://hdl.handle.net/11336/224927
identifier_str_mv Biological activities of essential oils on Caenorhabditis elegans: from molecular target to anthelmintic therapeutic strategies; 24th International C. elegans Conference; Glasgow; Escocia; 2023; 471-471
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://genetics-gsa.org/celegans2023/program-and-abstract-books/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Genetics Society of America
publisher.none.fl_str_mv Genetics Society of America
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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