Cys-loop receptors in Caenorhabditis elegans as pharmacological drug targets (Simposio)

Autores
Bouzat, Cecilia Beatriz
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The free-living nematode Caenorhabditis elegans has emerged as a powerful model for the study of the nervous system and human diseases and as a model for antiparasitic drug discovery. This nematode has also shown promise in the pharmaceutical industry search for new therapeutic compounds by high-throughput screening. Pentameric ligand gated ion channels, which include Cys-loop receptors, mediate rapid synaptic transmission by converting the chemical signal given by the neurotransmitter into an electrical one. These receptors play key roles in physiological processes, such as neuromuscular transmission, cognition, memory, and are targets of pharmacological compounds of clinical relevance. C. elegans has one of the most extended families of Cys-loop receptors, which have multiple functions including neuromuscular transmission. In particular, nicotinic (nAChR) and GABA receptors are essential for worm locomotion and are of clinical importance as targets of antiparasitic drugs. We combined paralysis assays, locomotion measurements and electrophysiological recordings from C. elegans cultured cells to identify the subunit composition, molecular function and antiparasitic drug modulation of muscle nAChR and GABA receptors. We also identified plant terpenoids and novel synthetic compounds that emerge as potential antiparasitic compounds by inducing rapid paralysis of C. elegans and deciphered the main drug targets and mechanisms underlying their anthelmintic actions. In order to use C. elegans as a model of human neuromuscular diseases, we generated transgenic strains containing mutant nAChRs that mimic those found in congenital myasthenic syndromes. We found that it is possible to recapitulate the molecular functional changes observed in patients, thus validating C. elegans as a model for these disorders.
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Reunión Anual de Sociedades de Biociencia: LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); LI Reunión Anual de la Asociación Argentina de Farmacología Experimental (SAFE); XXI Reunión Anual de la Sociedad Argentina de Biología (SAB); XXXI Reunión Anual de la Sociedad Argentina de Protozoología (SAP); IX Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-ar) y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL)
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
The Histochemical Society
Materia
CYS-LOOP RECEPTORS
CAENORHABDITIS ELEGANS
DRUG TARGET
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/161082
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/161082
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cys-loop receptors in Caenorhabditis elegans as pharmacological drug targets (Simposio)Bouzat, Cecilia BeatrizCYS-LOOP RECEPTORSCAENORHABDITIS ELEGANSDRUG TARGEThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The free-living nematode Caenorhabditis elegans has emerged as a powerful model for the study of the nervous system and human diseases and as a model for antiparasitic drug discovery. This nematode has also shown promise in the pharmaceutical industry search for new therapeutic compounds by high-throughput screening. Pentameric ligand gated ion channels, which include Cys-loop receptors, mediate rapid synaptic transmission by converting the chemical signal given by the neurotransmitter into an electrical one. These receptors play key roles in physiological processes, such as neuromuscular transmission, cognition, memory, and are targets of pharmacological compounds of clinical relevance. C. elegans has one of the most extended families of Cys-loop receptors, which have multiple functions including neuromuscular transmission. In particular, nicotinic (nAChR) and GABA receptors are essential for worm locomotion and are of clinical importance as targets of antiparasitic drugs. We combined paralysis assays, locomotion measurements and electrophysiological recordings from C. elegans cultured cells to identify the subunit composition, molecular function and antiparasitic drug modulation of muscle nAChR and GABA receptors. We also identified plant terpenoids and novel synthetic compounds that emerge as potential antiparasitic compounds by inducing rapid paralysis of C. elegans and deciphered the main drug targets and mechanisms underlying their anthelmintic actions. In order to use C. elegans as a model of human neuromuscular diseases, we generated transgenic strains containing mutant nAChRs that mimic those found in congenital myasthenic syndromes. We found that it is possible to recapitulate the molecular functional changes observed in patients, thus validating C. elegans as a model for these disorders.Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaReunión Anual de Sociedades de Biociencia: LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); LI Reunión Anual de la Asociación Argentina de Farmacología Experimental (SAFE); XXI Reunión Anual de la Sociedad Argentina de Biología (SAB); XXXI Reunión Anual de la Sociedad Argentina de Protozoología (SAP); IX Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-ar) y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL)Mar del PlataArgentinaSociedad Argentina de Investigación ClínicaAsociación Argentina de Farmacología ExperimentalSociedad Argentina de BiologíaSociedad Argentina de ProtozoologíaAsociación Argentina de NanomedicinasAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioThe Histochemical SocietyFundación Revista Medicina2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/161082Cys-loop receptors in Caenorhabditis elegans as pharmacological drug targets (Simposio); Reunión Anual de Sociedades de Biociencia: LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); LI Reunión Anual de la Asociación Argentina de Farmacología Experimental (SAFE); XXI Reunión Anual de la Sociedad Argentina de Biología (SAB); XXXI Reunión Anual de la Sociedad Argentina de Protozoología (SAP); IX Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-ar) y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL); Mar del Plata; Argentina; 2019; 36-360025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicinaNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:32:44Zoai:ri.conicet.gov.ar:11336/161082instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:32:44.904CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cys-loop receptors in Caenorhabditis elegans as pharmacological drug targets (Simposio)
title Cys-loop receptors in Caenorhabditis elegans as pharmacological drug targets (Simposio)
spellingShingle Cys-loop receptors in Caenorhabditis elegans as pharmacological drug targets (Simposio)
Bouzat, Cecilia Beatriz
CYS-LOOP RECEPTORS
CAENORHABDITIS ELEGANS
DRUG TARGET
title_short Cys-loop receptors in Caenorhabditis elegans as pharmacological drug targets (Simposio)
title_full Cys-loop receptors in Caenorhabditis elegans as pharmacological drug targets (Simposio)
title_fullStr Cys-loop receptors in Caenorhabditis elegans as pharmacological drug targets (Simposio)
title_full_unstemmed Cys-loop receptors in Caenorhabditis elegans as pharmacological drug targets (Simposio)
title_sort Cys-loop receptors in Caenorhabditis elegans as pharmacological drug targets (Simposio)
dc.creator.none.fl_str_mv Bouzat, Cecilia Beatriz
author Bouzat, Cecilia Beatriz
author_facet Bouzat, Cecilia Beatriz
author_role author
dc.subject.none.fl_str_mv CYS-LOOP RECEPTORS
CAENORHABDITIS ELEGANS
DRUG TARGET
topic CYS-LOOP RECEPTORS
CAENORHABDITIS ELEGANS
DRUG TARGET
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The free-living nematode Caenorhabditis elegans has emerged as a powerful model for the study of the nervous system and human diseases and as a model for antiparasitic drug discovery. This nematode has also shown promise in the pharmaceutical industry search for new therapeutic compounds by high-throughput screening. Pentameric ligand gated ion channels, which include Cys-loop receptors, mediate rapid synaptic transmission by converting the chemical signal given by the neurotransmitter into an electrical one. These receptors play key roles in physiological processes, such as neuromuscular transmission, cognition, memory, and are targets of pharmacological compounds of clinical relevance. C. elegans has one of the most extended families of Cys-loop receptors, which have multiple functions including neuromuscular transmission. In particular, nicotinic (nAChR) and GABA receptors are essential for worm locomotion and are of clinical importance as targets of antiparasitic drugs. We combined paralysis assays, locomotion measurements and electrophysiological recordings from C. elegans cultured cells to identify the subunit composition, molecular function and antiparasitic drug modulation of muscle nAChR and GABA receptors. We also identified plant terpenoids and novel synthetic compounds that emerge as potential antiparasitic compounds by inducing rapid paralysis of C. elegans and deciphered the main drug targets and mechanisms underlying their anthelmintic actions. In order to use C. elegans as a model of human neuromuscular diseases, we generated transgenic strains containing mutant nAChRs that mimic those found in congenital myasthenic syndromes. We found that it is possible to recapitulate the molecular functional changes observed in patients, thus validating C. elegans as a model for these disorders.
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Reunión Anual de Sociedades de Biociencia: LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); LI Reunión Anual de la Asociación Argentina de Farmacología Experimental (SAFE); XXI Reunión Anual de la Sociedad Argentina de Biología (SAB); XXXI Reunión Anual de la Sociedad Argentina de Protozoología (SAP); IX Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-ar) y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL)
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
The Histochemical Society
description The free-living nematode Caenorhabditis elegans has emerged as a powerful model for the study of the nervous system and human diseases and as a model for antiparasitic drug discovery. This nematode has also shown promise in the pharmaceutical industry search for new therapeutic compounds by high-throughput screening. Pentameric ligand gated ion channels, which include Cys-loop receptors, mediate rapid synaptic transmission by converting the chemical signal given by the neurotransmitter into an electrical one. These receptors play key roles in physiological processes, such as neuromuscular transmission, cognition, memory, and are targets of pharmacological compounds of clinical relevance. C. elegans has one of the most extended families of Cys-loop receptors, which have multiple functions including neuromuscular transmission. In particular, nicotinic (nAChR) and GABA receptors are essential for worm locomotion and are of clinical importance as targets of antiparasitic drugs. We combined paralysis assays, locomotion measurements and electrophysiological recordings from C. elegans cultured cells to identify the subunit composition, molecular function and antiparasitic drug modulation of muscle nAChR and GABA receptors. We also identified plant terpenoids and novel synthetic compounds that emerge as potential antiparasitic compounds by inducing rapid paralysis of C. elegans and deciphered the main drug targets and mechanisms underlying their anthelmintic actions. In order to use C. elegans as a model of human neuromuscular diseases, we generated transgenic strains containing mutant nAChRs that mimic those found in congenital myasthenic syndromes. We found that it is possible to recapitulate the molecular functional changes observed in patients, thus validating C. elegans as a model for these disorders.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/161082
Cys-loop receptors in Caenorhabditis elegans as pharmacological drug targets (Simposio); Reunión Anual de Sociedades de Biociencia: LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); LI Reunión Anual de la Asociación Argentina de Farmacología Experimental (SAFE); XXI Reunión Anual de la Sociedad Argentina de Biología (SAB); XXXI Reunión Anual de la Sociedad Argentina de Protozoología (SAP); IX Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-ar) y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL); Mar del Plata; Argentina; 2019; 36-36
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/161082
identifier_str_mv Cys-loop receptors in Caenorhabditis elegans as pharmacological drug targets (Simposio); Reunión Anual de Sociedades de Biociencia: LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); LI Reunión Anual de la Asociación Argentina de Farmacología Experimental (SAFE); XXI Reunión Anual de la Sociedad Argentina de Biología (SAB); XXXI Reunión Anual de la Sociedad Argentina de Protozoología (SAP); IX Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-ar) y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL); Mar del Plata; Argentina; 2019; 36-36
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicina
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
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dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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