C. Elegans as a screening plataform for drug discovery

Autores
Hernando, Guillermina Silvana; Turani, Ornella; Rodriguez Araujo, Noelia; Bouzat, Cecilia Beatriz
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The free-living nematode Caenorhabditis elegans is a widely utilized model organism in various fields of mod- ern biology. Its advantages, such as its easy manipulabil- ity, low maintenance cost, short reproductive cycle of ap- proximately three days, large brood size of 300 progenies per hermaphrodite worm, and transparent body, make it the ideal choice for drug discovery screenings. Notably, C. elegans possesses the largest known Cys-loop recep- tor family, along with unique receptors absent in verte- brates, which present attractive targets for anthelmintic drugs. Additionally, this nematode has shown promise in the search for new therapeutic compounds, including Essential Oils (EOs). EOs have found extensive appli- cations in human and veterinary health, with their active agents being isolated and incorporated into numerous pharmaceutical preparations. Our hypothesis states that EOs derived from aromatic plants commonly used in aro- matherapy or as food additives contain compounds with anthelmintic activity. To test this hypothesis, we utilize C. elegans as a model organism for parasitic nematodes. We have developed behavioral and molecular assays using both wild-type and mutant worms lacking Cys-loop receptors involved in locomotion. These assays aim to identify the primary compounds within EOs that mediate anthelmintic activities and their respective pharmacolog- ical targets. Furthermore, we explore the combination of current anthelmintics with active compounds from EOs as a strategy to mitigate drug resistance. Our objective is to provide a robust platform for the dis- covery of novel antiparasitic compounds. Through our research, we have identified six distinct EOs that inhibit C. elegans locomotion and egg hatching, each exhibiting varying levels of potency, thereby indicating their anthel- mintic capacity. Moreover, we have successfully identi- fied the key bioactive compounds and receptor targets associated with these EOs using single-channel and whole-cell recordings from cultured C. elegans muscle cells. These findings have allowed us to elucidate the molecular mechanisms behind the modulation of C. ele- gans nicotinic and GABA receptors by these compounds. In conclusion, our results highlight EOs as valuable sources of natural compounds with promising polyphar- macological profiles for anthelmintic therapies. We have deciphered the molecular basis of their actions and pro- vided insights into the efficacy of drug combinations as strategies to combat drug resistance in nematodes.
Fil: Hernando, Guillermina Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Turani, Ornella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Rodriguez Araujo, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Materia
CAENORHABDITIS ELEGANS
ESSENTIAL OILS
CYS-LOOP RECEPTORS
DRUG DISCOVERY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/223866
Acceder
id CONICETDig_678b6db62ae74a79074c754db83b7338
oai_identifier_str oai:ri.conicet.gov.ar:11336/223866
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling C. Elegans as a screening plataform for drug discoveryHernando, Guillermina SilvanaTurani, OrnellaRodriguez Araujo, NoeliaBouzat, Cecilia BeatrizCAENORHABDITIS ELEGANSESSENTIAL OILSCYS-LOOP RECEPTORSDRUG DISCOVERYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The free-living nematode Caenorhabditis elegans is a widely utilized model organism in various fields of mod- ern biology. Its advantages, such as its easy manipulabil- ity, low maintenance cost, short reproductive cycle of ap- proximately three days, large brood size of 300 progenies per hermaphrodite worm, and transparent body, make it the ideal choice for drug discovery screenings. Notably, C. elegans possesses the largest known Cys-loop recep- tor family, along with unique receptors absent in verte- brates, which present attractive targets for anthelmintic drugs. Additionally, this nematode has shown promise in the search for new therapeutic compounds, including Essential Oils (EOs). EOs have found extensive appli- cations in human and veterinary health, with their active agents being isolated and incorporated into numerous pharmaceutical preparations. Our hypothesis states that EOs derived from aromatic plants commonly used in aro- matherapy or as food additives contain compounds with anthelmintic activity. To test this hypothesis, we utilize C. elegans as a model organism for parasitic nematodes. We have developed behavioral and molecular assays using both wild-type and mutant worms lacking Cys-loop receptors involved in locomotion. These assays aim to identify the primary compounds within EOs that mediate anthelmintic activities and their respective pharmacolog- ical targets. Furthermore, we explore the combination of current anthelmintics with active compounds from EOs as a strategy to mitigate drug resistance. Our objective is to provide a robust platform for the dis- covery of novel antiparasitic compounds. Through our research, we have identified six distinct EOs that inhibit C. elegans locomotion and egg hatching, each exhibiting varying levels of potency, thereby indicating their anthel- mintic capacity. Moreover, we have successfully identi- fied the key bioactive compounds and receptor targets associated with these EOs using single-channel and whole-cell recordings from cultured C. elegans muscle cells. These findings have allowed us to elucidate the molecular mechanisms behind the modulation of C. ele- gans nicotinic and GABA receptors by these compounds. In conclusion, our results highlight EOs as valuable sources of natural compounds with promising polyphar- macological profiles for anthelmintic therapies. We have deciphered the molecular basis of their actions and pro- vided insights into the efficacy of drug combinations as strategies to combat drug resistance in nematodes.Fil: Hernando, Guillermina Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Turani, Ornella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Rodriguez Araujo, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaLXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de LaboratorioMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de BiologíaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioFundación Revista Medicina2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/223866C. Elegans as a screening plataform for drug discovery; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 55-551669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicinaNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:31Zoai:ri.conicet.gov.ar:11336/223866instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:31.336CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv C. Elegans as a screening plataform for drug discovery
title C. Elegans as a screening plataform for drug discovery
spellingShingle C. Elegans as a screening plataform for drug discovery
Hernando, Guillermina Silvana
CAENORHABDITIS ELEGANS
ESSENTIAL OILS
CYS-LOOP RECEPTORS
DRUG DISCOVERY
title_short C. Elegans as a screening plataform for drug discovery
title_full C. Elegans as a screening plataform for drug discovery
title_fullStr C. Elegans as a screening plataform for drug discovery
title_full_unstemmed C. Elegans as a screening plataform for drug discovery
title_sort C. Elegans as a screening plataform for drug discovery
dc.creator.none.fl_str_mv Hernando, Guillermina Silvana
Turani, Ornella
Rodriguez Araujo, Noelia
Bouzat, Cecilia Beatriz
author Hernando, Guillermina Silvana
author_facet Hernando, Guillermina Silvana
Turani, Ornella
Rodriguez Araujo, Noelia
Bouzat, Cecilia Beatriz
author_role author
author2 Turani, Ornella
Rodriguez Araujo, Noelia
Bouzat, Cecilia Beatriz
author2_role author
author
author
dc.subject.none.fl_str_mv CAENORHABDITIS ELEGANS
ESSENTIAL OILS
CYS-LOOP RECEPTORS
DRUG DISCOVERY
topic CAENORHABDITIS ELEGANS
ESSENTIAL OILS
CYS-LOOP RECEPTORS
DRUG DISCOVERY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The free-living nematode Caenorhabditis elegans is a widely utilized model organism in various fields of mod- ern biology. Its advantages, such as its easy manipulabil- ity, low maintenance cost, short reproductive cycle of ap- proximately three days, large brood size of 300 progenies per hermaphrodite worm, and transparent body, make it the ideal choice for drug discovery screenings. Notably, C. elegans possesses the largest known Cys-loop recep- tor family, along with unique receptors absent in verte- brates, which present attractive targets for anthelmintic drugs. Additionally, this nematode has shown promise in the search for new therapeutic compounds, including Essential Oils (EOs). EOs have found extensive appli- cations in human and veterinary health, with their active agents being isolated and incorporated into numerous pharmaceutical preparations. Our hypothesis states that EOs derived from aromatic plants commonly used in aro- matherapy or as food additives contain compounds with anthelmintic activity. To test this hypothesis, we utilize C. elegans as a model organism for parasitic nematodes. We have developed behavioral and molecular assays using both wild-type and mutant worms lacking Cys-loop receptors involved in locomotion. These assays aim to identify the primary compounds within EOs that mediate anthelmintic activities and their respective pharmacolog- ical targets. Furthermore, we explore the combination of current anthelmintics with active compounds from EOs as a strategy to mitigate drug resistance. Our objective is to provide a robust platform for the dis- covery of novel antiparasitic compounds. Through our research, we have identified six distinct EOs that inhibit C. elegans locomotion and egg hatching, each exhibiting varying levels of potency, thereby indicating their anthel- mintic capacity. Moreover, we have successfully identi- fied the key bioactive compounds and receptor targets associated with these EOs using single-channel and whole-cell recordings from cultured C. elegans muscle cells. These findings have allowed us to elucidate the molecular mechanisms behind the modulation of C. ele- gans nicotinic and GABA receptors by these compounds. In conclusion, our results highlight EOs as valuable sources of natural compounds with promising polyphar- macological profiles for anthelmintic therapies. We have deciphered the molecular basis of their actions and pro- vided insights into the efficacy of drug combinations as strategies to combat drug resistance in nematodes.
Fil: Hernando, Guillermina Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Turani, Ornella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Rodriguez Araujo, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
description The free-living nematode Caenorhabditis elegans is a widely utilized model organism in various fields of mod- ern biology. Its advantages, such as its easy manipulabil- ity, low maintenance cost, short reproductive cycle of ap- proximately three days, large brood size of 300 progenies per hermaphrodite worm, and transparent body, make it the ideal choice for drug discovery screenings. Notably, C. elegans possesses the largest known Cys-loop recep- tor family, along with unique receptors absent in verte- brates, which present attractive targets for anthelmintic drugs. Additionally, this nematode has shown promise in the search for new therapeutic compounds, including Essential Oils (EOs). EOs have found extensive appli- cations in human and veterinary health, with their active agents being isolated and incorporated into numerous pharmaceutical preparations. Our hypothesis states that EOs derived from aromatic plants commonly used in aro- matherapy or as food additives contain compounds with anthelmintic activity. To test this hypothesis, we utilize C. elegans as a model organism for parasitic nematodes. We have developed behavioral and molecular assays using both wild-type and mutant worms lacking Cys-loop receptors involved in locomotion. These assays aim to identify the primary compounds within EOs that mediate anthelmintic activities and their respective pharmacolog- ical targets. Furthermore, we explore the combination of current anthelmintics with active compounds from EOs as a strategy to mitigate drug resistance. Our objective is to provide a robust platform for the dis- covery of novel antiparasitic compounds. Through our research, we have identified six distinct EOs that inhibit C. elegans locomotion and egg hatching, each exhibiting varying levels of potency, thereby indicating their anthel- mintic capacity. Moreover, we have successfully identi- fied the key bioactive compounds and receptor targets associated with these EOs using single-channel and whole-cell recordings from cultured C. elegans muscle cells. These findings have allowed us to elucidate the molecular mechanisms behind the modulation of C. ele- gans nicotinic and GABA receptors by these compounds. In conclusion, our results highlight EOs as valuable sources of natural compounds with promising polyphar- macological profiles for anthelmintic therapies. We have deciphered the molecular basis of their actions and pro- vided insights into the efficacy of drug combinations as strategies to combat drug resistance in nematodes.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/223866
C. Elegans as a screening plataform for drug discovery; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 55-55
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/223866
identifier_str_mv C. Elegans as a screening plataform for drug discovery; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 55-55
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicina
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268977558978560
score 13.13397

Publicaciones similares