Degradation and detoxification of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) by an indigenous Delftia sp. strain in batch and continuous systems

Autores
González, Ana Julieta; Gallego, Alfredo; Gemini, V. L.; Papalia, Mariana Andrea; Radice, Marcela Alejandra; Gutkind, Gabriel Osvaldo; Planes, Estela; Korol, Sonia Edith
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An indigenous bacterial strain capable of utilizing 2,4-dichlorophenoxyacetic acid (2,4-D) as the sole carbon and energy source was isolated from a polluted river in Buenos Aires, Argentina. The strain was identified as Delftia sp. by the 16S rRNA gene sequencing. Aerobic biodegradation assays in batch process were performed using a 2 L volume microfermentor at 28 C with agitation (200 rpm). The continuousflow system employed consists in a down-flow fixed-bed reactor, using polyurethane foam cubes as a support for immobilizing bacterial cells. Degradation of 2,4-D was evaluated by UV spectrophotometry, chemical oxygen demand (COD) and gas chromatography (GC). In the batch reactor microbial growth was measured by the plate count method. Biofilm development on polyurethane foam was observed by scanning electronic microscopy. Detoxification was assessed by using seeds toxicity tests. Under batch conditions Delftia sp. was able to degrade 100 and 200 mg L1 of 2,4-D in 24 and 28 h respectively. Compound and COD removals were 99.9% and 91.3% respectively at an initial 2,4-D concentration of 100 mg L1, and 99.0% and 89.4% when 2,4-D concentration was increased to 200 mg L1. When assays were performed in the continuous-flow reactor under operating conditions 2,4-D and COD removals of 99.6% and 90.8% were achieved, respectively. Toxicity was neither detected at the end of the batch process nor in the continuous-flow reactor effluent.
Fil: González, Ana Julieta. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gallego, Alfredo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; Argentina
Fil: Gemini, V. L.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; Argentina
Fil: Papalia, Mariana Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Radice, Marcela Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Planes, Estela. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Korol, Sonia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; Argentina
Materia
2,4-D
Delftia sp.
Biodegradation
Detoxification
Continuous reactor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/268919
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/268919
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Degradation and detoxification of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) by an indigenous Delftia sp. strain in batch and continuous systemsGonzález, Ana JulietaGallego, AlfredoGemini, V. L.Papalia, Mariana AndreaRadice, Marcela AlejandraGutkind, Gabriel OsvaldoPlanes, EstelaKorol, Sonia Edith2,4-DDelftia sp.BiodegradationDetoxificationContinuous reactorhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2An indigenous bacterial strain capable of utilizing 2,4-dichlorophenoxyacetic acid (2,4-D) as the sole carbon and energy source was isolated from a polluted river in Buenos Aires, Argentina. The strain was identified as Delftia sp. by the 16S rRNA gene sequencing. Aerobic biodegradation assays in batch process were performed using a 2 L volume microfermentor at 28 C with agitation (200 rpm). The continuousflow system employed consists in a down-flow fixed-bed reactor, using polyurethane foam cubes as a support for immobilizing bacterial cells. Degradation of 2,4-D was evaluated by UV spectrophotometry, chemical oxygen demand (COD) and gas chromatography (GC). In the batch reactor microbial growth was measured by the plate count method. Biofilm development on polyurethane foam was observed by scanning electronic microscopy. Detoxification was assessed by using seeds toxicity tests. Under batch conditions Delftia sp. was able to degrade 100 and 200 mg L1 of 2,4-D in 24 and 28 h respectively. Compound and COD removals were 99.9% and 91.3% respectively at an initial 2,4-D concentration of 100 mg L1, and 99.0% and 89.4% when 2,4-D concentration was increased to 200 mg L1. When assays were performed in the continuous-flow reactor under operating conditions 2,4-D and COD removals of 99.6% and 90.8% were achieved, respectively. Toxicity was neither detected at the end of the batch process nor in the continuous-flow reactor effluent.Fil: González, Ana Julieta. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gallego, Alfredo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; ArgentinaFil: Gemini, V. L.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; ArgentinaFil: Papalia, Mariana Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Radice, Marcela Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Planes, Estela. Instituto Nacional de Tecnología Industrial; ArgentinaFil: Korol, Sonia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; ArgentinaElsevier2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/268919González, Ana Julieta; Gallego, Alfredo; Gemini, V. L.; Papalia, Mariana Andrea; Radice, Marcela Alejandra; et al.; Degradation and detoxification of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) by an indigenous Delftia sp. strain in batch and continuous systems; Elsevier; International Biodeterioration and Biodegradation; 66; 1; 1-2012; 8-130964-8305CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S096483051100206Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2011.09.010info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:00:36Zoai:ri.conicet.gov.ar:11336/268919instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:00:36.366CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Degradation and detoxification of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) by an indigenous Delftia sp. strain in batch and continuous systems
title Degradation and detoxification of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) by an indigenous Delftia sp. strain in batch and continuous systems
spellingShingle Degradation and detoxification of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) by an indigenous Delftia sp. strain in batch and continuous systems
González, Ana Julieta
2,4-D
Delftia sp.
Biodegradation
Detoxification
Continuous reactor
title_short Degradation and detoxification of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) by an indigenous Delftia sp. strain in batch and continuous systems
title_full Degradation and detoxification of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) by an indigenous Delftia sp. strain in batch and continuous systems
title_fullStr Degradation and detoxification of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) by an indigenous Delftia sp. strain in batch and continuous systems
title_full_unstemmed Degradation and detoxification of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) by an indigenous Delftia sp. strain in batch and continuous systems
title_sort Degradation and detoxification of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) by an indigenous Delftia sp. strain in batch and continuous systems
dc.creator.none.fl_str_mv González, Ana Julieta
Gallego, Alfredo
Gemini, V. L.
Papalia, Mariana Andrea
Radice, Marcela Alejandra
Gutkind, Gabriel Osvaldo
Planes, Estela
Korol, Sonia Edith
author González, Ana Julieta
author_facet González, Ana Julieta
Gallego, Alfredo
Gemini, V. L.
Papalia, Mariana Andrea
Radice, Marcela Alejandra
Gutkind, Gabriel Osvaldo
Planes, Estela
Korol, Sonia Edith
author_role author
author2 Gallego, Alfredo
Gemini, V. L.
Papalia, Mariana Andrea
Radice, Marcela Alejandra
Gutkind, Gabriel Osvaldo
Planes, Estela
Korol, Sonia Edith
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv 2,4-D
Delftia sp.
Biodegradation
Detoxification
Continuous reactor
topic 2,4-D
Delftia sp.
Biodegradation
Detoxification
Continuous reactor
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv An indigenous bacterial strain capable of utilizing 2,4-dichlorophenoxyacetic acid (2,4-D) as the sole carbon and energy source was isolated from a polluted river in Buenos Aires, Argentina. The strain was identified as Delftia sp. by the 16S rRNA gene sequencing. Aerobic biodegradation assays in batch process were performed using a 2 L volume microfermentor at 28 C with agitation (200 rpm). The continuousflow system employed consists in a down-flow fixed-bed reactor, using polyurethane foam cubes as a support for immobilizing bacterial cells. Degradation of 2,4-D was evaluated by UV spectrophotometry, chemical oxygen demand (COD) and gas chromatography (GC). In the batch reactor microbial growth was measured by the plate count method. Biofilm development on polyurethane foam was observed by scanning electronic microscopy. Detoxification was assessed by using seeds toxicity tests. Under batch conditions Delftia sp. was able to degrade 100 and 200 mg L1 of 2,4-D in 24 and 28 h respectively. Compound and COD removals were 99.9% and 91.3% respectively at an initial 2,4-D concentration of 100 mg L1, and 99.0% and 89.4% when 2,4-D concentration was increased to 200 mg L1. When assays were performed in the continuous-flow reactor under operating conditions 2,4-D and COD removals of 99.6% and 90.8% were achieved, respectively. Toxicity was neither detected at the end of the batch process nor in the continuous-flow reactor effluent.
Fil: González, Ana Julieta. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gallego, Alfredo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; Argentina
Fil: Gemini, V. L.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; Argentina
Fil: Papalia, Mariana Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Radice, Marcela Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Planes, Estela. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Korol, Sonia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; Argentina
description An indigenous bacterial strain capable of utilizing 2,4-dichlorophenoxyacetic acid (2,4-D) as the sole carbon and energy source was isolated from a polluted river in Buenos Aires, Argentina. The strain was identified as Delftia sp. by the 16S rRNA gene sequencing. Aerobic biodegradation assays in batch process were performed using a 2 L volume microfermentor at 28 C with agitation (200 rpm). The continuousflow system employed consists in a down-flow fixed-bed reactor, using polyurethane foam cubes as a support for immobilizing bacterial cells. Degradation of 2,4-D was evaluated by UV spectrophotometry, chemical oxygen demand (COD) and gas chromatography (GC). In the batch reactor microbial growth was measured by the plate count method. Biofilm development on polyurethane foam was observed by scanning electronic microscopy. Detoxification was assessed by using seeds toxicity tests. Under batch conditions Delftia sp. was able to degrade 100 and 200 mg L1 of 2,4-D in 24 and 28 h respectively. Compound and COD removals were 99.9% and 91.3% respectively at an initial 2,4-D concentration of 100 mg L1, and 99.0% and 89.4% when 2,4-D concentration was increased to 200 mg L1. When assays were performed in the continuous-flow reactor under operating conditions 2,4-D and COD removals of 99.6% and 90.8% were achieved, respectively. Toxicity was neither detected at the end of the batch process nor in the continuous-flow reactor effluent.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/268919
González, Ana Julieta; Gallego, Alfredo; Gemini, V. L.; Papalia, Mariana Andrea; Radice, Marcela Alejandra; et al.; Degradation and detoxification of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) by an indigenous Delftia sp. strain in batch and continuous systems; Elsevier; International Biodeterioration and Biodegradation; 66; 1; 1-2012; 8-13
0964-8305
CONICET Digital
CONICET
url http://hdl.handle.net/11336/268919
identifier_str_mv González, Ana Julieta; Gallego, Alfredo; Gemini, V. L.; Papalia, Mariana Andrea; Radice, Marcela Alejandra; et al.; Degradation and detoxification of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) by an indigenous Delftia sp. strain in batch and continuous systems; Elsevier; International Biodeterioration and Biodegradation; 66; 1; 1-2012; 8-13
0964-8305
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S096483051100206X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2011.09.010
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
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reponame_str CONICET Digital (CONICET)
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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