Selection and identification of a bacterial community able to degrade and detoxify m-nitrophenol in continuous biofilm reactors
- Autores
- González, Ana Julieta; Fortunato, María Susana; Papalia, Mariana Andrea; Radice, Marcela Alejandra; Gutkind, Gabriel Osvaldo; Magdaleno, Anahí; Gallego, Alfredo; Korol, Sonia Edith
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Nitroaromatics are widely used for industrial purposes and constitute a group of compounds of environmental concern because of their persistence and toxic properties. Biological processes used for decontamination of nitroaromatic-polluted sources have then attracted worldwide attention. In the present investigation m-nitrophenol (MNP) biodegradation was studied in batch and continuous reactors. A bacterial community able to degrade the compound was first selected from a polluted freshwater stream and the isolates were identified by the analysis of the 16S rRNA gene sequence. The bacterial community was then used in biodegradation assays. Batch experiments were conducted in a 2L aerobic microfermentor at 28°C and with agitation (200rpm). The influence of abiotic factors in the biodegradation process in batch reactors, such as initial concentration of the compound and initial pH of the medium, was also studied. Continuous degradation of MNP was performed in an aerobic up-flow fixed-bed biofilm reactor. The biodegradation process was evaluated by determining MNP and ammonium concentrations and chemical oxygen demand (COD). Detoxification was assessed by Vibrio fischeri and Pseudokirchneriella subcapitata toxicity tests. Under batch conditions the bacterial community was able to degrade 0.72mM of MNP in 32h, with efficiencies higher than 99.9% and 89.0% of MNP and COD removals respectively and with concomitant release of ammonium. When the initial MNP concentration increased to 1.08 and 1.44mM MNP the biodegradation process was accomplished in 40 and 44h, respectively. No biodegradation of the compound was observed at higher concentrations. The community was also able to degrade 0.72mM of the compound at pH 5, 7 and 9. In the continuous process biodegradation efficiency reached 99.5% and 96.8% of MNP and COD removal respectively. The maximum MNP removal rate was 37.9gm-3day-1. Toxicity was not detected after the biodegradation process.
Fil: González, Ana Julieta. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fortunato, María Susana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; Argentina
Fil: Papalia, Mariana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina
Fil: Radice, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina
Fil: Gutkind, Gabriel Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina
Fil: Magdaleno, Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; Argentina
Fil: Gallego, Alfredo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Korol, Sonia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
Abiotic Factors
Biodegradation
Continuous Biofilm Reactors
Detoxification
M-Nitrophenol - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/37940
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Selection and identification of a bacterial community able to degrade and detoxify m-nitrophenol in continuous biofilm reactorsGonzález, Ana JulietaFortunato, María SusanaPapalia, Mariana AndreaRadice, Marcela AlejandraGutkind, Gabriel OsvaldoMagdaleno, AnahíGallego, AlfredoKorol, Sonia EdithAbiotic FactorsBiodegradationContinuous Biofilm ReactorsDetoxificationM-Nitrophenolhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Nitroaromatics are widely used for industrial purposes and constitute a group of compounds of environmental concern because of their persistence and toxic properties. Biological processes used for decontamination of nitroaromatic-polluted sources have then attracted worldwide attention. In the present investigation m-nitrophenol (MNP) biodegradation was studied in batch and continuous reactors. A bacterial community able to degrade the compound was first selected from a polluted freshwater stream and the isolates were identified by the analysis of the 16S rRNA gene sequence. The bacterial community was then used in biodegradation assays. Batch experiments were conducted in a 2L aerobic microfermentor at 28°C and with agitation (200rpm). The influence of abiotic factors in the biodegradation process in batch reactors, such as initial concentration of the compound and initial pH of the medium, was also studied. Continuous degradation of MNP was performed in an aerobic up-flow fixed-bed biofilm reactor. The biodegradation process was evaluated by determining MNP and ammonium concentrations and chemical oxygen demand (COD). Detoxification was assessed by Vibrio fischeri and Pseudokirchneriella subcapitata toxicity tests. Under batch conditions the bacterial community was able to degrade 0.72mM of MNP in 32h, with efficiencies higher than 99.9% and 89.0% of MNP and COD removals respectively and with concomitant release of ammonium. When the initial MNP concentration increased to 1.08 and 1.44mM MNP the biodegradation process was accomplished in 40 and 44h, respectively. No biodegradation of the compound was observed at higher concentrations. The community was also able to degrade 0.72mM of the compound at pH 5, 7 and 9. In the continuous process biodegradation efficiency reached 99.5% and 96.8% of MNP and COD removal respectively. The maximum MNP removal rate was 37.9gm-3day-1. Toxicity was not detected after the biodegradation process.Fil: González, Ana Julieta. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fortunato, María Susana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; ArgentinaFil: Papalia, Mariana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; ArgentinaFil: Radice, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; ArgentinaFil: Gutkind, Gabriel Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; ArgentinaFil: Magdaleno, Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; ArgentinaFil: Gallego, Alfredo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Korol, Sonia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaAcademic Press Inc Elsevier Science2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37940González, Ana Julieta; Fortunato, María Susana; Papalia, Mariana Andrea; Radice, Marcela Alejandra; Gutkind, Gabriel Osvaldo; et al.; Selection and identification of a bacterial community able to degrade and detoxify m-nitrophenol in continuous biofilm reactors; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 122; 12-2015; 245-2510147-6513CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0147651315300361info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2015.07.029info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:45:15Zoai:ri.conicet.gov.ar:11336/37940instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:45:15.678CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Selection and identification of a bacterial community able to degrade and detoxify m-nitrophenol in continuous biofilm reactors |
title |
Selection and identification of a bacterial community able to degrade and detoxify m-nitrophenol in continuous biofilm reactors |
spellingShingle |
Selection and identification of a bacterial community able to degrade and detoxify m-nitrophenol in continuous biofilm reactors González, Ana Julieta Abiotic Factors Biodegradation Continuous Biofilm Reactors Detoxification M-Nitrophenol |
title_short |
Selection and identification of a bacterial community able to degrade and detoxify m-nitrophenol in continuous biofilm reactors |
title_full |
Selection and identification of a bacterial community able to degrade and detoxify m-nitrophenol in continuous biofilm reactors |
title_fullStr |
Selection and identification of a bacterial community able to degrade and detoxify m-nitrophenol in continuous biofilm reactors |
title_full_unstemmed |
Selection and identification of a bacterial community able to degrade and detoxify m-nitrophenol in continuous biofilm reactors |
title_sort |
Selection and identification of a bacterial community able to degrade and detoxify m-nitrophenol in continuous biofilm reactors |
dc.creator.none.fl_str_mv |
González, Ana Julieta Fortunato, María Susana Papalia, Mariana Andrea Radice, Marcela Alejandra Gutkind, Gabriel Osvaldo Magdaleno, Anahí Gallego, Alfredo Korol, Sonia Edith |
author |
González, Ana Julieta |
author_facet |
González, Ana Julieta Fortunato, María Susana Papalia, Mariana Andrea Radice, Marcela Alejandra Gutkind, Gabriel Osvaldo Magdaleno, Anahí Gallego, Alfredo Korol, Sonia Edith |
author_role |
author |
author2 |
Fortunato, María Susana Papalia, Mariana Andrea Radice, Marcela Alejandra Gutkind, Gabriel Osvaldo Magdaleno, Anahí Gallego, Alfredo Korol, Sonia Edith |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Abiotic Factors Biodegradation Continuous Biofilm Reactors Detoxification M-Nitrophenol |
topic |
Abiotic Factors Biodegradation Continuous Biofilm Reactors Detoxification M-Nitrophenol |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.8 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Nitroaromatics are widely used for industrial purposes and constitute a group of compounds of environmental concern because of their persistence and toxic properties. Biological processes used for decontamination of nitroaromatic-polluted sources have then attracted worldwide attention. In the present investigation m-nitrophenol (MNP) biodegradation was studied in batch and continuous reactors. A bacterial community able to degrade the compound was first selected from a polluted freshwater stream and the isolates were identified by the analysis of the 16S rRNA gene sequence. The bacterial community was then used in biodegradation assays. Batch experiments were conducted in a 2L aerobic microfermentor at 28°C and with agitation (200rpm). The influence of abiotic factors in the biodegradation process in batch reactors, such as initial concentration of the compound and initial pH of the medium, was also studied. Continuous degradation of MNP was performed in an aerobic up-flow fixed-bed biofilm reactor. The biodegradation process was evaluated by determining MNP and ammonium concentrations and chemical oxygen demand (COD). Detoxification was assessed by Vibrio fischeri and Pseudokirchneriella subcapitata toxicity tests. Under batch conditions the bacterial community was able to degrade 0.72mM of MNP in 32h, with efficiencies higher than 99.9% and 89.0% of MNP and COD removals respectively and with concomitant release of ammonium. When the initial MNP concentration increased to 1.08 and 1.44mM MNP the biodegradation process was accomplished in 40 and 44h, respectively. No biodegradation of the compound was observed at higher concentrations. The community was also able to degrade 0.72mM of the compound at pH 5, 7 and 9. In the continuous process biodegradation efficiency reached 99.5% and 96.8% of MNP and COD removal respectively. The maximum MNP removal rate was 37.9gm-3day-1. Toxicity was not detected after the biodegradation process. Fil: González, Ana Julieta. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fortunato, María Susana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; Argentina Fil: Papalia, Mariana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina Fil: Radice, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina Fil: Gutkind, Gabriel Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina Fil: Magdaleno, Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; Argentina Fil: Gallego, Alfredo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Korol, Sonia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Sanidad, Nutrición, Bromatología y Toxicología. Cátedra de Higiene y Sanidad; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Nitroaromatics are widely used for industrial purposes and constitute a group of compounds of environmental concern because of their persistence and toxic properties. Biological processes used for decontamination of nitroaromatic-polluted sources have then attracted worldwide attention. In the present investigation m-nitrophenol (MNP) biodegradation was studied in batch and continuous reactors. A bacterial community able to degrade the compound was first selected from a polluted freshwater stream and the isolates were identified by the analysis of the 16S rRNA gene sequence. The bacterial community was then used in biodegradation assays. Batch experiments were conducted in a 2L aerobic microfermentor at 28°C and with agitation (200rpm). The influence of abiotic factors in the biodegradation process in batch reactors, such as initial concentration of the compound and initial pH of the medium, was also studied. Continuous degradation of MNP was performed in an aerobic up-flow fixed-bed biofilm reactor. The biodegradation process was evaluated by determining MNP and ammonium concentrations and chemical oxygen demand (COD). Detoxification was assessed by Vibrio fischeri and Pseudokirchneriella subcapitata toxicity tests. Under batch conditions the bacterial community was able to degrade 0.72mM of MNP in 32h, with efficiencies higher than 99.9% and 89.0% of MNP and COD removals respectively and with concomitant release of ammonium. When the initial MNP concentration increased to 1.08 and 1.44mM MNP the biodegradation process was accomplished in 40 and 44h, respectively. No biodegradation of the compound was observed at higher concentrations. The community was also able to degrade 0.72mM of the compound at pH 5, 7 and 9. In the continuous process biodegradation efficiency reached 99.5% and 96.8% of MNP and COD removal respectively. The maximum MNP removal rate was 37.9gm-3day-1. Toxicity was not detected after the biodegradation process. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/37940 González, Ana Julieta; Fortunato, María Susana; Papalia, Mariana Andrea; Radice, Marcela Alejandra; Gutkind, Gabriel Osvaldo; et al.; Selection and identification of a bacterial community able to degrade and detoxify m-nitrophenol in continuous biofilm reactors; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 122; 12-2015; 245-251 0147-6513 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/37940 |
identifier_str_mv |
González, Ana Julieta; Fortunato, María Susana; Papalia, Mariana Andrea; Radice, Marcela Alejandra; Gutkind, Gabriel Osvaldo; et al.; Selection and identification of a bacterial community able to degrade and detoxify m-nitrophenol in continuous biofilm reactors; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 122; 12-2015; 245-251 0147-6513 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0147651315300361 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2015.07.029 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Academic Press Inc Elsevier Science |
publisher.none.fl_str_mv |
Academic Press Inc Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |