Evidence of a noncoding transcript of the RIPK2 gene overexpressed in head and neck tumor

Autores
Mancini Villagra, Ulises Maximiliano; da Cunha, Bianca R.; Polachini, Giovana M.; Tiago, Tiago Henrique; Carlos H. T. P. da Silva; Feitosa, Olavo A.; Fukuyama, Erica E.; López, Rossana V. M.; Dias Neto, Emmanuel; Nunes, Fabio D.; Severino, Patricia; Tajara, Eloiza Helena Tajara
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Receptor-interacting proteins are a family of serine/threonine kinases, which integrate extra and intracellular stress signals caused by different factors, including infections, inflammation and DNA damage. Receptor-interacting serine/threonine-protein kinase 2 (RIP-2) is a member of this family and an important component of the nuclear factor NF-kappa-B signaling pathway. The corresponding human gene RIPK2 generates two transcripts by alternative splicing, the full-length and a short transcript. The short transcript has a truncated 5? sequence, which results in a predicted isoform with a partial kinase domain but able to transduce signals through its caspase recruitment domain. In this study, the expression of RIPK2 was investigated in human tissue samples and, in order to determine if both transcripts are similarly regulated at the transcriptional level, cancer cell lines were submitted to temperature and acid stresses. We observed that both transcripts are expressed in all tissues analyzed, with higher expression of the short one in tumor samples, and they are differentially regulated following temperature stress. Despite transcription, no corresponding protein for the short transcript was detected in tissues and cell lines analyzed. We propose that the shorter transcript is a noncoding RNA and that its presence in the cell may play regulatory roles and affect inflammation and other biological processes related to the kinase activity of RIP-2.
Fil: Mancini Villagra, Ulises Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: da Cunha, Bianca R.. Universidade de Sao Paulo; Brasil
Fil: Polachini, Giovana M.. No especifíca;
Fil: Tiago, Tiago Henrique. No especifíca;
Fil: Carlos H. T. P. da Silva. Universidade de Sao Paulo; Brasil
Fil: Feitosa, Olavo A.. Universidade de Sao Paulo; Brasil
Fil: Fukuyama, Erica E.. Arnaldo Vieira de Carvalho Cancer Institute; Brasil
Fil: López, Rossana V. M.. No especifíca;
Fil: Dias Neto, Emmanuel. Universidade de Sao Paulo; Brasil
Fil: Nunes, Fabio D.. Universidade de Sao Paulo; Brasil
Fil: Severino, Patricia. Hospital Israelita Albert Einstein; Brasil
Fil: Tajara, Eloiza Helena Tajara. Universidade de Sao Paulo; Brasil
Materia
RIPK2
ALTERNATIVE SPLICING
HEAD AND NECK CANCER
TEMPERATURE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/180761
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/180761
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evidence of a noncoding transcript of the RIPK2 gene overexpressed in head and neck tumorMancini Villagra, Ulises Maximilianoda Cunha, Bianca R.Polachini, Giovana M.Tiago, Tiago HenriqueCarlos H. T. P. da SilvaFeitosa, Olavo A.Fukuyama, Erica E.López, Rossana V. M.Dias Neto, EmmanuelNunes, Fabio D.Severino, PatriciaTajara, Eloiza Helena TajaraRIPK2ALTERNATIVE SPLICINGHEAD AND NECK CANCERTEMPERATUREhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Receptor-interacting proteins are a family of serine/threonine kinases, which integrate extra and intracellular stress signals caused by different factors, including infections, inflammation and DNA damage. Receptor-interacting serine/threonine-protein kinase 2 (RIP-2) is a member of this family and an important component of the nuclear factor NF-kappa-B signaling pathway. The corresponding human gene RIPK2 generates two transcripts by alternative splicing, the full-length and a short transcript. The short transcript has a truncated 5? sequence, which results in a predicted isoform with a partial kinase domain but able to transduce signals through its caspase recruitment domain. In this study, the expression of RIPK2 was investigated in human tissue samples and, in order to determine if both transcripts are similarly regulated at the transcriptional level, cancer cell lines were submitted to temperature and acid stresses. We observed that both transcripts are expressed in all tissues analyzed, with higher expression of the short one in tumor samples, and they are differentially regulated following temperature stress. Despite transcription, no corresponding protein for the short transcript was detected in tissues and cell lines analyzed. We propose that the shorter transcript is a noncoding RNA and that its presence in the cell may play regulatory roles and affect inflammation and other biological processes related to the kinase activity of RIP-2.Fil: Mancini Villagra, Ulises Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: da Cunha, Bianca R.. Universidade de Sao Paulo; BrasilFil: Polachini, Giovana M.. No especifíca;Fil: Tiago, Tiago Henrique. No especifíca;Fil: Carlos H. T. P. da Silva. Universidade de Sao Paulo; BrasilFil: Feitosa, Olavo A.. Universidade de Sao Paulo; BrasilFil: Fukuyama, Erica E.. Arnaldo Vieira de Carvalho Cancer Institute; BrasilFil: López, Rossana V. M.. No especifíca;Fil: Dias Neto, Emmanuel. Universidade de Sao Paulo; BrasilFil: Nunes, Fabio D.. Universidade de Sao Paulo; BrasilFil: Severino, Patricia. Hospital Israelita Albert Einstein; BrasilFil: Tajara, Eloiza Helena Tajara. Universidade de Sao Paulo; BrasilCold Spring Harbor Laboratory Press2018-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/180761Mancini Villagra, Ulises Maximiliano; da Cunha, Bianca R. ; Polachini, Giovana M.; Tiago, Tiago Henrique; Carlos H. T. P. da Silva; et al.; Evidence of a noncoding transcript of the RIPK2 gene overexpressed in head and neck tumor; Cold Spring Harbor Laboratory Press; bioRxiv.; 11-2018; 1-282692-8205CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.biorxiv.org/content/10.1101/466011v1info:eu-repo/semantics/altIdentifier/doi/10.1101/466011info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:59Zoai:ri.conicet.gov.ar:11336/180761instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:59.476CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evidence of a noncoding transcript of the RIPK2 gene overexpressed in head and neck tumor
title Evidence of a noncoding transcript of the RIPK2 gene overexpressed in head and neck tumor
spellingShingle Evidence of a noncoding transcript of the RIPK2 gene overexpressed in head and neck tumor
Mancini Villagra, Ulises Maximiliano
RIPK2
ALTERNATIVE SPLICING
HEAD AND NECK CANCER
TEMPERATURE
title_short Evidence of a noncoding transcript of the RIPK2 gene overexpressed in head and neck tumor
title_full Evidence of a noncoding transcript of the RIPK2 gene overexpressed in head and neck tumor
title_fullStr Evidence of a noncoding transcript of the RIPK2 gene overexpressed in head and neck tumor
title_full_unstemmed Evidence of a noncoding transcript of the RIPK2 gene overexpressed in head and neck tumor
title_sort Evidence of a noncoding transcript of the RIPK2 gene overexpressed in head and neck tumor
dc.creator.none.fl_str_mv Mancini Villagra, Ulises Maximiliano
da Cunha, Bianca R.
Polachini, Giovana M.
Tiago, Tiago Henrique
Carlos H. T. P. da Silva
Feitosa, Olavo A.
Fukuyama, Erica E.
López, Rossana V. M.
Dias Neto, Emmanuel
Nunes, Fabio D.
Severino, Patricia
Tajara, Eloiza Helena Tajara
author Mancini Villagra, Ulises Maximiliano
author_facet Mancini Villagra, Ulises Maximiliano
da Cunha, Bianca R.
Polachini, Giovana M.
Tiago, Tiago Henrique
Carlos H. T. P. da Silva
Feitosa, Olavo A.
Fukuyama, Erica E.
López, Rossana V. M.
Dias Neto, Emmanuel
Nunes, Fabio D.
Severino, Patricia
Tajara, Eloiza Helena Tajara
author_role author
author2 da Cunha, Bianca R.
Polachini, Giovana M.
Tiago, Tiago Henrique
Carlos H. T. P. da Silva
Feitosa, Olavo A.
Fukuyama, Erica E.
López, Rossana V. M.
Dias Neto, Emmanuel
Nunes, Fabio D.
Severino, Patricia
Tajara, Eloiza Helena Tajara
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv RIPK2
ALTERNATIVE SPLICING
HEAD AND NECK CANCER
TEMPERATURE
topic RIPK2
ALTERNATIVE SPLICING
HEAD AND NECK CANCER
TEMPERATURE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Receptor-interacting proteins are a family of serine/threonine kinases, which integrate extra and intracellular stress signals caused by different factors, including infections, inflammation and DNA damage. Receptor-interacting serine/threonine-protein kinase 2 (RIP-2) is a member of this family and an important component of the nuclear factor NF-kappa-B signaling pathway. The corresponding human gene RIPK2 generates two transcripts by alternative splicing, the full-length and a short transcript. The short transcript has a truncated 5? sequence, which results in a predicted isoform with a partial kinase domain but able to transduce signals through its caspase recruitment domain. In this study, the expression of RIPK2 was investigated in human tissue samples and, in order to determine if both transcripts are similarly regulated at the transcriptional level, cancer cell lines were submitted to temperature and acid stresses. We observed that both transcripts are expressed in all tissues analyzed, with higher expression of the short one in tumor samples, and they are differentially regulated following temperature stress. Despite transcription, no corresponding protein for the short transcript was detected in tissues and cell lines analyzed. We propose that the shorter transcript is a noncoding RNA and that its presence in the cell may play regulatory roles and affect inflammation and other biological processes related to the kinase activity of RIP-2.
Fil: Mancini Villagra, Ulises Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: da Cunha, Bianca R.. Universidade de Sao Paulo; Brasil
Fil: Polachini, Giovana M.. No especifíca;
Fil: Tiago, Tiago Henrique. No especifíca;
Fil: Carlos H. T. P. da Silva. Universidade de Sao Paulo; Brasil
Fil: Feitosa, Olavo A.. Universidade de Sao Paulo; Brasil
Fil: Fukuyama, Erica E.. Arnaldo Vieira de Carvalho Cancer Institute; Brasil
Fil: López, Rossana V. M.. No especifíca;
Fil: Dias Neto, Emmanuel. Universidade de Sao Paulo; Brasil
Fil: Nunes, Fabio D.. Universidade de Sao Paulo; Brasil
Fil: Severino, Patricia. Hospital Israelita Albert Einstein; Brasil
Fil: Tajara, Eloiza Helena Tajara. Universidade de Sao Paulo; Brasil
description Receptor-interacting proteins are a family of serine/threonine kinases, which integrate extra and intracellular stress signals caused by different factors, including infections, inflammation and DNA damage. Receptor-interacting serine/threonine-protein kinase 2 (RIP-2) is a member of this family and an important component of the nuclear factor NF-kappa-B signaling pathway. The corresponding human gene RIPK2 generates two transcripts by alternative splicing, the full-length and a short transcript. The short transcript has a truncated 5? sequence, which results in a predicted isoform with a partial kinase domain but able to transduce signals through its caspase recruitment domain. In this study, the expression of RIPK2 was investigated in human tissue samples and, in order to determine if both transcripts are similarly regulated at the transcriptional level, cancer cell lines were submitted to temperature and acid stresses. We observed that both transcripts are expressed in all tissues analyzed, with higher expression of the short one in tumor samples, and they are differentially regulated following temperature stress. Despite transcription, no corresponding protein for the short transcript was detected in tissues and cell lines analyzed. We propose that the shorter transcript is a noncoding RNA and that its presence in the cell may play regulatory roles and affect inflammation and other biological processes related to the kinase activity of RIP-2.
publishDate 2018
dc.date.none.fl_str_mv 2018-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/180761
Mancini Villagra, Ulises Maximiliano; da Cunha, Bianca R. ; Polachini, Giovana M.; Tiago, Tiago Henrique; Carlos H. T. P. da Silva; et al.; Evidence of a noncoding transcript of the RIPK2 gene overexpressed in head and neck tumor; Cold Spring Harbor Laboratory Press; bioRxiv.; 11-2018; 1-28
2692-8205
CONICET Digital
CONICET
url http://hdl.handle.net/11336/180761
identifier_str_mv Mancini Villagra, Ulises Maximiliano; da Cunha, Bianca R. ; Polachini, Giovana M.; Tiago, Tiago Henrique; Carlos H. T. P. da Silva; et al.; Evidence of a noncoding transcript of the RIPK2 gene overexpressed in head and neck tumor; Cold Spring Harbor Laboratory Press; bioRxiv.; 11-2018; 1-28
2692-8205
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.biorxiv.org/content/10.1101/466011v1
info:eu-repo/semantics/altIdentifier/doi/10.1101/466011
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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