Expression of Truncated Products at the 5′-Terminal Region of RIPK2 and Evolutive Aspects that Support Their Biological Importance

Autores
Mancini Villagra, Ulises Maximiliano; da Cunha, Bianca R.; Polachini, Giovana M.; Henrique, Tiago; Buzzo Stefanini, Ana Carolina; de Castro, Tialfi Bergamin; da Silva, Carlos H. T. P.; Feitosa, Olavo A.; Fukuyama, Erica E.; López, Rossana V. M.; Dias Neto, Emmanuel; Nunes, Fabio D.; Severino, Patricia; Tajara, Eloiza H.
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Alternative splicing is the process of generating different mRNAs from the same primary transcript, which contributes to increase the transcriptome and proteome diversity. Abnormal splicing has been associated with the development of several diseases including cancer. Given that mutations and abnormal levels of the RIPK2 transcript and RIP-2 protein are frequent in tumors, and that RIP-2 modulates immune and inflammatory responses, we investigated alternative splicing events that result in partial deletions of the kinase domain at the N-terminus of RIP-2. We also investigated the structure and expression of the RIPK2 truncated variants and isoforms in different environments. In addition, we searched data throughout Supraprimates evolution that could support the biological importance of RIPK2 alternatively spliced products. We observed that human variants and isoforms were differentially regulated following temperature stress, and that the truncated transcript was more expressed than the long transcript in tumor samples. The inverse was found for the longer protein isoform. The truncated variant was also detected in chimpanzee, gorilla, hare, pika, mouse, rat, and tree shrew. The fact that the same variant has been preserved in mammals with divergence times up to 70 million years raises the hypothesis that it may have a functional significance.
Fil: Mancini Villagra, Ulises Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: da Cunha, Bianca R.. Universidade de Sao Paulo; Brasil
Fil: Polachini, Giovana M.. Universidade de Sao Paulo; Brasil
Fil: Henrique, Tiago. Universidade de Sao Paulo; Brasil
Fil: Buzzo Stefanini, Ana Carolina. Universidade de Sao Paulo; Brasil
Fil: de Castro, Tialfi Bergamin. Universidade de Sao Paulo; Brasil. University of Maryland; Estados Unidos
Fil: da Silva, Carlos H. T. P.. Universidade de Sao Paulo; Brasil
Fil: Feitosa, Olavo A.. Universidade de Sao Paulo; Brasil
Fil: Fukuyama, Erica E.. Arnaldo Vieira de Carvalho Cancer Institute; Brasil
Fil: López, Rossana V. M.. Universidade de Sao Paulo; Brasil
Fil: Dias Neto, Emmanuel. A.C. Camargo Cancer Center; Brasil
Fil: Nunes, Fabio D.. Universidade de Sao Paulo; Brasil
Fil: Severino, Patricia. Universidade de Sao Paulo; Brasil. Hospital Israelita Albert Einstein; Brasil
Fil: Tajara, Eloiza H.. Universidade de Sao Paulo; Brasil
Materia
Alternative splicing
RIPK2
Gene expression and stress conditions
Isoform conservation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/269075
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/269075
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression of Truncated Products at the 5′-Terminal Region of RIPK2 and Evolutive Aspects that Support Their Biological ImportanceMancini Villagra, Ulises Maximilianoda Cunha, Bianca R.Polachini, Giovana M.Henrique, TiagoBuzzo Stefanini, Ana Carolinade Castro, Tialfi Bergaminda Silva, Carlos H. T. P.Feitosa, Olavo A.Fukuyama, Erica E.López, Rossana V. M.Dias Neto, EmmanuelNunes, Fabio D.Severino, PatriciaTajara, Eloiza H.Alternative splicingRIPK2Gene expression and stress conditionsIsoform conservationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Alternative splicing is the process of generating different mRNAs from the same primary transcript, which contributes to increase the transcriptome and proteome diversity. Abnormal splicing has been associated with the development of several diseases including cancer. Given that mutations and abnormal levels of the RIPK2 transcript and RIP-2 protein are frequent in tumors, and that RIP-2 modulates immune and inflammatory responses, we investigated alternative splicing events that result in partial deletions of the kinase domain at the N-terminus of RIP-2. We also investigated the structure and expression of the RIPK2 truncated variants and isoforms in different environments. In addition, we searched data throughout Supraprimates evolution that could support the biological importance of RIPK2 alternatively spliced products. We observed that human variants and isoforms were differentially regulated following temperature stress, and that the truncated transcript was more expressed than the long transcript in tumor samples. The inverse was found for the longer protein isoform. The truncated variant was also detected in chimpanzee, gorilla, hare, pika, mouse, rat, and tree shrew. The fact that the same variant has been preserved in mammals with divergence times up to 70 million years raises the hypothesis that it may have a functional significance.Fil: Mancini Villagra, Ulises Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: da Cunha, Bianca R.. Universidade de Sao Paulo; BrasilFil: Polachini, Giovana M.. Universidade de Sao Paulo; BrasilFil: Henrique, Tiago. Universidade de Sao Paulo; BrasilFil: Buzzo Stefanini, Ana Carolina. Universidade de Sao Paulo; BrasilFil: de Castro, Tialfi Bergamin. Universidade de Sao Paulo; Brasil. University of Maryland; Estados UnidosFil: da Silva, Carlos H. T. P.. Universidade de Sao Paulo; BrasilFil: Feitosa, Olavo A.. Universidade de Sao Paulo; BrasilFil: Fukuyama, Erica E.. Arnaldo Vieira de Carvalho Cancer Institute; BrasilFil: López, Rossana V. M.. Universidade de Sao Paulo; BrasilFil: Dias Neto, Emmanuel. A.C. Camargo Cancer Center; BrasilFil: Nunes, Fabio D.. Universidade de Sao Paulo; BrasilFil: Severino, Patricia. Universidade de Sao Paulo; Brasil. Hospital Israelita Albert Einstein; BrasilFil: Tajara, Eloiza H.. Universidade de Sao Paulo; BrasilOxford University Press2024-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/269075Mancini Villagra, Ulises Maximiliano; da Cunha, Bianca R.; Polachini, Giovana M.; Henrique, Tiago; Buzzo Stefanini, Ana Carolina; et al.; Expression of Truncated Products at the 5′-Terminal Region of RIPK2 and Evolutive Aspects that Support Their Biological Importance; Oxford University Press; Genome Biology and Evolution; 16; 7; 7-2024; 1-201759-6653CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/gbe/article/doi/10.1093/gbe/evae106/7675095info:eu-repo/semantics/altIdentifier/doi/10.1093/gbe/evae106info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:38:55Zoai:ri.conicet.gov.ar:11336/269075instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:38:55.434CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression of Truncated Products at the 5′-Terminal Region of RIPK2 and Evolutive Aspects that Support Their Biological Importance
title Expression of Truncated Products at the 5′-Terminal Region of RIPK2 and Evolutive Aspects that Support Their Biological Importance
spellingShingle Expression of Truncated Products at the 5′-Terminal Region of RIPK2 and Evolutive Aspects that Support Their Biological Importance
Mancini Villagra, Ulises Maximiliano
Alternative splicing
RIPK2
Gene expression and stress conditions
Isoform conservation
title_short Expression of Truncated Products at the 5′-Terminal Region of RIPK2 and Evolutive Aspects that Support Their Biological Importance
title_full Expression of Truncated Products at the 5′-Terminal Region of RIPK2 and Evolutive Aspects that Support Their Biological Importance
title_fullStr Expression of Truncated Products at the 5′-Terminal Region of RIPK2 and Evolutive Aspects that Support Their Biological Importance
title_full_unstemmed Expression of Truncated Products at the 5′-Terminal Region of RIPK2 and Evolutive Aspects that Support Their Biological Importance
title_sort Expression of Truncated Products at the 5′-Terminal Region of RIPK2 and Evolutive Aspects that Support Their Biological Importance
dc.creator.none.fl_str_mv Mancini Villagra, Ulises Maximiliano
da Cunha, Bianca R.
Polachini, Giovana M.
Henrique, Tiago
Buzzo Stefanini, Ana Carolina
de Castro, Tialfi Bergamin
da Silva, Carlos H. T. P.
Feitosa, Olavo A.
Fukuyama, Erica E.
López, Rossana V. M.
Dias Neto, Emmanuel
Nunes, Fabio D.
Severino, Patricia
Tajara, Eloiza H.
author Mancini Villagra, Ulises Maximiliano
author_facet Mancini Villagra, Ulises Maximiliano
da Cunha, Bianca R.
Polachini, Giovana M.
Henrique, Tiago
Buzzo Stefanini, Ana Carolina
de Castro, Tialfi Bergamin
da Silva, Carlos H. T. P.
Feitosa, Olavo A.
Fukuyama, Erica E.
López, Rossana V. M.
Dias Neto, Emmanuel
Nunes, Fabio D.
Severino, Patricia
Tajara, Eloiza H.
author_role author
author2 da Cunha, Bianca R.
Polachini, Giovana M.
Henrique, Tiago
Buzzo Stefanini, Ana Carolina
de Castro, Tialfi Bergamin
da Silva, Carlos H. T. P.
Feitosa, Olavo A.
Fukuyama, Erica E.
López, Rossana V. M.
Dias Neto, Emmanuel
Nunes, Fabio D.
Severino, Patricia
Tajara, Eloiza H.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Alternative splicing
RIPK2
Gene expression and stress conditions
Isoform conservation
topic Alternative splicing
RIPK2
Gene expression and stress conditions
Isoform conservation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Alternative splicing is the process of generating different mRNAs from the same primary transcript, which contributes to increase the transcriptome and proteome diversity. Abnormal splicing has been associated with the development of several diseases including cancer. Given that mutations and abnormal levels of the RIPK2 transcript and RIP-2 protein are frequent in tumors, and that RIP-2 modulates immune and inflammatory responses, we investigated alternative splicing events that result in partial deletions of the kinase domain at the N-terminus of RIP-2. We also investigated the structure and expression of the RIPK2 truncated variants and isoforms in different environments. In addition, we searched data throughout Supraprimates evolution that could support the biological importance of RIPK2 alternatively spliced products. We observed that human variants and isoforms were differentially regulated following temperature stress, and that the truncated transcript was more expressed than the long transcript in tumor samples. The inverse was found for the longer protein isoform. The truncated variant was also detected in chimpanzee, gorilla, hare, pika, mouse, rat, and tree shrew. The fact that the same variant has been preserved in mammals with divergence times up to 70 million years raises the hypothesis that it may have a functional significance.
Fil: Mancini Villagra, Ulises Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: da Cunha, Bianca R.. Universidade de Sao Paulo; Brasil
Fil: Polachini, Giovana M.. Universidade de Sao Paulo; Brasil
Fil: Henrique, Tiago. Universidade de Sao Paulo; Brasil
Fil: Buzzo Stefanini, Ana Carolina. Universidade de Sao Paulo; Brasil
Fil: de Castro, Tialfi Bergamin. Universidade de Sao Paulo; Brasil. University of Maryland; Estados Unidos
Fil: da Silva, Carlos H. T. P.. Universidade de Sao Paulo; Brasil
Fil: Feitosa, Olavo A.. Universidade de Sao Paulo; Brasil
Fil: Fukuyama, Erica E.. Arnaldo Vieira de Carvalho Cancer Institute; Brasil
Fil: López, Rossana V. M.. Universidade de Sao Paulo; Brasil
Fil: Dias Neto, Emmanuel. A.C. Camargo Cancer Center; Brasil
Fil: Nunes, Fabio D.. Universidade de Sao Paulo; Brasil
Fil: Severino, Patricia. Universidade de Sao Paulo; Brasil. Hospital Israelita Albert Einstein; Brasil
Fil: Tajara, Eloiza H.. Universidade de Sao Paulo; Brasil
description Alternative splicing is the process of generating different mRNAs from the same primary transcript, which contributes to increase the transcriptome and proteome diversity. Abnormal splicing has been associated with the development of several diseases including cancer. Given that mutations and abnormal levels of the RIPK2 transcript and RIP-2 protein are frequent in tumors, and that RIP-2 modulates immune and inflammatory responses, we investigated alternative splicing events that result in partial deletions of the kinase domain at the N-terminus of RIP-2. We also investigated the structure and expression of the RIPK2 truncated variants and isoforms in different environments. In addition, we searched data throughout Supraprimates evolution that could support the biological importance of RIPK2 alternatively spliced products. We observed that human variants and isoforms were differentially regulated following temperature stress, and that the truncated transcript was more expressed than the long transcript in tumor samples. The inverse was found for the longer protein isoform. The truncated variant was also detected in chimpanzee, gorilla, hare, pika, mouse, rat, and tree shrew. The fact that the same variant has been preserved in mammals with divergence times up to 70 million years raises the hypothesis that it may have a functional significance.
publishDate 2024
dc.date.none.fl_str_mv 2024-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/269075
Mancini Villagra, Ulises Maximiliano; da Cunha, Bianca R.; Polachini, Giovana M.; Henrique, Tiago; Buzzo Stefanini, Ana Carolina; et al.; Expression of Truncated Products at the 5′-Terminal Region of RIPK2 and Evolutive Aspects that Support Their Biological Importance; Oxford University Press; Genome Biology and Evolution; 16; 7; 7-2024; 1-20
1759-6653
CONICET Digital
CONICET
url http://hdl.handle.net/11336/269075
identifier_str_mv Mancini Villagra, Ulises Maximiliano; da Cunha, Bianca R.; Polachini, Giovana M.; Henrique, Tiago; Buzzo Stefanini, Ana Carolina; et al.; Expression of Truncated Products at the 5′-Terminal Region of RIPK2 and Evolutive Aspects that Support Their Biological Importance; Oxford University Press; Genome Biology and Evolution; 16; 7; 7-2024; 1-20
1759-6653
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/gbe/article/doi/10.1093/gbe/evae106/7675095
info:eu-repo/semantics/altIdentifier/doi/10.1093/gbe/evae106
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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