Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos ( Lama guanicoe ) and its application in a wild population

Autores
Marozzi, Antonela Alejandra; Cantarelli, Verónica Inés; Panebianco, Antonella; Gomez, Facundo Martin; Ovejero, R.; Gregorio, Pablo Francisco; Peña, Fiama Evelin; Ponzio, Marina Flavia; Carmanchahi, Pablo Daniel
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Obtaining endocrinological profiles using non-invasive methodologies by the measurement of hormone fecal metabolites is awidely used method to monitor ovarian activity and pregnancy in wild species. These tools allow the obtention of physiologicalinformation without causing capture-related stress on the individuals. In this research, we aimed to 1) biologically validate anon-invasive method to assess fecal progestagens and estrogens fluctuations during gestation in guanacos (Lama guanicoe)and 2) apply this technique to assess pregnancy in a wild free-ranging population. Fecal samples were collected through thegestation period (∼12 months) of female guanacos in a 6.5-ha paddock. An increase in fecal metabolites of both hormoneswas detected. Progestagens increased gradually, in contrast to estrogens, which remained at basal values for most of thegestation period and peaked only a few days before calving. To assess pregnancy in wild free-ranging animals, fecal sampleswere collected from a population of La Payunia provincial reserve (Mendoza, Argentina) during the beginning of gestation andat the end of gestation. Through the first months of possible gestation, pregnant females represented between 40 and 80% ofthe population; at the end of gestation, only 20–40% of the females had confirmed pregnancies. Our results demonstrated thatthe polyclonal antisera and sexual hormone metabolite assays used here detect variations in the metabolites excreted throughfeces in guanacos and provide the possibility of non-invasive hormone monitoring of female reproductive status. Also, thefindings in wild conditions suggest that natural abortions could have occurred during the first months of gestation. Althoughsome abortions may be natural, the harsh environmental conditions that challenge the support of such a long gestationalprocess may be another relevant factor to consider. The results obtained here enhance our understanding of the reproductivephysiology of one of the most emblematic ungulates in South America.
Fil: Marozzi, Antonela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Cantarelli, Verónica Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Panebianco, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Gomez, Facundo Martin. Universidad Nacional del Comahue. Asentamiento Universidad San Martín de Los Andes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ovejero, R.. Universidad Nacional de Tucumán. Instituto de Ecología Regional. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Ecología Regional; Argentina
Fil: Gregorio, Pablo Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Peña, Fiama Evelin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina
Fil: Ponzio, Marina Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Carmanchahi, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Materia
Estrone conjugates
gestation
pregnancy rate
pregnanediol glucuronides
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/260913
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos ( Lama guanicoe ) and its application in a wild populationMarozzi, Antonela AlejandraCantarelli, Verónica InésPanebianco, AntonellaGomez, Facundo MartinOvejero, R.Gregorio, Pablo FranciscoPeña, Fiama EvelinPonzio, Marina FlaviaCarmanchahi, Pablo DanielEstrone conjugatesgestationpregnancy ratepregnanediol glucuronideshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Obtaining endocrinological profiles using non-invasive methodologies by the measurement of hormone fecal metabolites is awidely used method to monitor ovarian activity and pregnancy in wild species. These tools allow the obtention of physiologicalinformation without causing capture-related stress on the individuals. In this research, we aimed to 1) biologically validate anon-invasive method to assess fecal progestagens and estrogens fluctuations during gestation in guanacos (Lama guanicoe)and 2) apply this technique to assess pregnancy in a wild free-ranging population. Fecal samples were collected through thegestation period (∼12 months) of female guanacos in a 6.5-ha paddock. An increase in fecal metabolites of both hormoneswas detected. Progestagens increased gradually, in contrast to estrogens, which remained at basal values for most of thegestation period and peaked only a few days before calving. To assess pregnancy in wild free-ranging animals, fecal sampleswere collected from a population of La Payunia provincial reserve (Mendoza, Argentina) during the beginning of gestation andat the end of gestation. Through the first months of possible gestation, pregnant females represented between 40 and 80% ofthe population; at the end of gestation, only 20–40% of the females had confirmed pregnancies. Our results demonstrated thatthe polyclonal antisera and sexual hormone metabolite assays used here detect variations in the metabolites excreted throughfeces in guanacos and provide the possibility of non-invasive hormone monitoring of female reproductive status. Also, thefindings in wild conditions suggest that natural abortions could have occurred during the first months of gestation. Althoughsome abortions may be natural, the harsh environmental conditions that challenge the support of such a long gestationalprocess may be another relevant factor to consider. The results obtained here enhance our understanding of the reproductivephysiology of one of the most emblematic ungulates in South America.Fil: Marozzi, Antonela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaFil: Cantarelli, Verónica Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Panebianco, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaFil: Gomez, Facundo Martin. Universidad Nacional del Comahue. Asentamiento Universidad San Martín de Los Andes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ovejero, R.. Universidad Nacional de Tucumán. Instituto de Ecología Regional. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Ecología Regional; ArgentinaFil: Gregorio, Pablo Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaFil: Peña, Fiama Evelin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; ArgentinaFil: Ponzio, Marina Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Carmanchahi, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaOxford University Press2024-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/260913Marozzi, Antonela Alejandra; Cantarelli, Verónica Inés; Panebianco, Antonella; Gomez, Facundo Martin; Ovejero, R.; et al.; Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos ( Lama guanicoe ) and its application in a wild population; Oxford University Press; Conservation Physiology; 12; 1; 2-2024; 1-112051-1434CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/conphys/article/doi/10.1093/conphys/coae003/7604205info:eu-repo/semantics/altIdentifier/doi/10.1093/conphys/coae003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:02:57Zoai:ri.conicet.gov.ar:11336/260913instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:02:58.177CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos ( Lama guanicoe ) and its application in a wild population
title Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos ( Lama guanicoe ) and its application in a wild population
spellingShingle Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos ( Lama guanicoe ) and its application in a wild population
Marozzi, Antonela Alejandra
Estrone conjugates
gestation
pregnancy rate
pregnanediol glucuronides
title_short Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos ( Lama guanicoe ) and its application in a wild population
title_full Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos ( Lama guanicoe ) and its application in a wild population
title_fullStr Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos ( Lama guanicoe ) and its application in a wild population
title_full_unstemmed Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos ( Lama guanicoe ) and its application in a wild population
title_sort Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos ( Lama guanicoe ) and its application in a wild population
dc.creator.none.fl_str_mv Marozzi, Antonela Alejandra
Cantarelli, Verónica Inés
Panebianco, Antonella
Gomez, Facundo Martin
Ovejero, R.
Gregorio, Pablo Francisco
Peña, Fiama Evelin
Ponzio, Marina Flavia
Carmanchahi, Pablo Daniel
author Marozzi, Antonela Alejandra
author_facet Marozzi, Antonela Alejandra
Cantarelli, Verónica Inés
Panebianco, Antonella
Gomez, Facundo Martin
Ovejero, R.
Gregorio, Pablo Francisco
Peña, Fiama Evelin
Ponzio, Marina Flavia
Carmanchahi, Pablo Daniel
author_role author
author2 Cantarelli, Verónica Inés
Panebianco, Antonella
Gomez, Facundo Martin
Ovejero, R.
Gregorio, Pablo Francisco
Peña, Fiama Evelin
Ponzio, Marina Flavia
Carmanchahi, Pablo Daniel
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Estrone conjugates
gestation
pregnancy rate
pregnanediol glucuronides
topic Estrone conjugates
gestation
pregnancy rate
pregnanediol glucuronides
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Obtaining endocrinological profiles using non-invasive methodologies by the measurement of hormone fecal metabolites is awidely used method to monitor ovarian activity and pregnancy in wild species. These tools allow the obtention of physiologicalinformation without causing capture-related stress on the individuals. In this research, we aimed to 1) biologically validate anon-invasive method to assess fecal progestagens and estrogens fluctuations during gestation in guanacos (Lama guanicoe)and 2) apply this technique to assess pregnancy in a wild free-ranging population. Fecal samples were collected through thegestation period (∼12 months) of female guanacos in a 6.5-ha paddock. An increase in fecal metabolites of both hormoneswas detected. Progestagens increased gradually, in contrast to estrogens, which remained at basal values for most of thegestation period and peaked only a few days before calving. To assess pregnancy in wild free-ranging animals, fecal sampleswere collected from a population of La Payunia provincial reserve (Mendoza, Argentina) during the beginning of gestation andat the end of gestation. Through the first months of possible gestation, pregnant females represented between 40 and 80% ofthe population; at the end of gestation, only 20–40% of the females had confirmed pregnancies. Our results demonstrated thatthe polyclonal antisera and sexual hormone metabolite assays used here detect variations in the metabolites excreted throughfeces in guanacos and provide the possibility of non-invasive hormone monitoring of female reproductive status. Also, thefindings in wild conditions suggest that natural abortions could have occurred during the first months of gestation. Althoughsome abortions may be natural, the harsh environmental conditions that challenge the support of such a long gestationalprocess may be another relevant factor to consider. The results obtained here enhance our understanding of the reproductivephysiology of one of the most emblematic ungulates in South America.
Fil: Marozzi, Antonela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Cantarelli, Verónica Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Panebianco, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Gomez, Facundo Martin. Universidad Nacional del Comahue. Asentamiento Universidad San Martín de Los Andes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ovejero, R.. Universidad Nacional de Tucumán. Instituto de Ecología Regional. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Ecología Regional; Argentina
Fil: Gregorio, Pablo Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Peña, Fiama Evelin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina
Fil: Ponzio, Marina Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Carmanchahi, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
description Obtaining endocrinological profiles using non-invasive methodologies by the measurement of hormone fecal metabolites is awidely used method to monitor ovarian activity and pregnancy in wild species. These tools allow the obtention of physiologicalinformation without causing capture-related stress on the individuals. In this research, we aimed to 1) biologically validate anon-invasive method to assess fecal progestagens and estrogens fluctuations during gestation in guanacos (Lama guanicoe)and 2) apply this technique to assess pregnancy in a wild free-ranging population. Fecal samples were collected through thegestation period (∼12 months) of female guanacos in a 6.5-ha paddock. An increase in fecal metabolites of both hormoneswas detected. Progestagens increased gradually, in contrast to estrogens, which remained at basal values for most of thegestation period and peaked only a few days before calving. To assess pregnancy in wild free-ranging animals, fecal sampleswere collected from a population of La Payunia provincial reserve (Mendoza, Argentina) during the beginning of gestation andat the end of gestation. Through the first months of possible gestation, pregnant females represented between 40 and 80% ofthe population; at the end of gestation, only 20–40% of the females had confirmed pregnancies. Our results demonstrated thatthe polyclonal antisera and sexual hormone metabolite assays used here detect variations in the metabolites excreted throughfeces in guanacos and provide the possibility of non-invasive hormone monitoring of female reproductive status. Also, thefindings in wild conditions suggest that natural abortions could have occurred during the first months of gestation. Althoughsome abortions may be natural, the harsh environmental conditions that challenge the support of such a long gestationalprocess may be another relevant factor to consider. The results obtained here enhance our understanding of the reproductivephysiology of one of the most emblematic ungulates in South America.
publishDate 2024
dc.date.none.fl_str_mv 2024-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/260913
Marozzi, Antonela Alejandra; Cantarelli, Verónica Inés; Panebianco, Antonella; Gomez, Facundo Martin; Ovejero, R.; et al.; Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos ( Lama guanicoe ) and its application in a wild population; Oxford University Press; Conservation Physiology; 12; 1; 2-2024; 1-11
2051-1434
CONICET Digital
CONICET
url http://hdl.handle.net/11336/260913
identifier_str_mv Marozzi, Antonela Alejandra; Cantarelli, Verónica Inés; Panebianco, Antonella; Gomez, Facundo Martin; Ovejero, R.; et al.; Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos ( Lama guanicoe ) and its application in a wild population; Oxford University Press; Conservation Physiology; 12; 1; 2-2024; 1-11
2051-1434
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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