Histone demethylase KDM4B regulates otic vesicle invagination via epigenetic control of Dlx3 expression

Autores
Uribe, Rosa A.; Buzzi, Ailín Leticia; Bronner, Marianne E.; Strobl Mazulla, Pablo Hernan
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In vertebrates, the inner ear arises from the otic placode, a thickened swathe of ectoderm that invaginates to form the otic vesicle. We report that histone demethylase KDM4B is dynamically expressed during early stages of chick inner ear formation. A loss of KDM4B results in defective invagination and striking morphological changes in the otic epithelium, characterized by abnormal localization of adhesion and cytoskeletal molecules and reduced expression of several inner ear markers, including Dlx3. In vivo chromatin immunoprecipitation reveals direct and dynamic occupancy of KDM4B and its target, H3K9me3, at regulatory regions of the Dlx3 locus. Accordingly, coelectroporations of DLX3 or KDM4B encoding constructs, but not a catalytically dead mutant of KDM4B, rescue the ear invagination phenotype caused by KDM4B knockdown. Moreover, a loss of DLX3 phenocopies a loss of KDM4B. Collectively, our findings suggest that KDM4B play a critical role during inner ear invagination via modulating histone methylation of the direct target Dlx3.
Fil: Uribe, Rosa A.. California Institute of Technology; Estados Unidos
Fil: Buzzi, Ailín Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Fil: Bronner, Marianne E.. California Institute of Technology; Estados Unidos
Fil: Strobl Mazulla, Pablo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Materia
Epigenetics
Inner ear
Invagination
Dlx3
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53679
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/53679
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Histone demethylase KDM4B regulates otic vesicle invagination via epigenetic control of Dlx3 expressionUribe, Rosa A.Buzzi, Ailín LeticiaBronner, Marianne E.Strobl Mazulla, Pablo HernanEpigeneticsInner earInvaginationDlx3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In vertebrates, the inner ear arises from the otic placode, a thickened swathe of ectoderm that invaginates to form the otic vesicle. We report that histone demethylase KDM4B is dynamically expressed during early stages of chick inner ear formation. A loss of KDM4B results in defective invagination and striking morphological changes in the otic epithelium, characterized by abnormal localization of adhesion and cytoskeletal molecules and reduced expression of several inner ear markers, including Dlx3. In vivo chromatin immunoprecipitation reveals direct and dynamic occupancy of KDM4B and its target, H3K9me3, at regulatory regions of the Dlx3 locus. Accordingly, coelectroporations of DLX3 or KDM4B encoding constructs, but not a catalytically dead mutant of KDM4B, rescue the ear invagination phenotype caused by KDM4B knockdown. Moreover, a loss of DLX3 phenocopies a loss of KDM4B. Collectively, our findings suggest that KDM4B play a critical role during inner ear invagination via modulating histone methylation of the direct target Dlx3.Fil: Uribe, Rosa A.. California Institute of Technology; Estados UnidosFil: Buzzi, Ailín Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; ArgentinaFil: Bronner, Marianne E.. California Institute of Technology; Estados UnidosFil: Strobl Mazulla, Pablo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; ArgentinaRockefeller University Press2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53679Uribe, Rosa A.; Buzzi, Ailín Leticia; Bronner, Marianne E.; Strobl Mazulla, Pablo Hernan; Histone demethylase KDM4B regulates otic vesicle invagination via epigenetic control of Dlx3 expression; Rockefeller University Press; Journal of Cell Biology; 211; 4; 11-2015; 815-8270021-9525CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1083/jcb.201503071info:eu-repo/semantics/altIdentifier/url/http://jcb.rupress.org/content/211/4/815info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:49:33Zoai:ri.conicet.gov.ar:11336/53679instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:49:33.421CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Histone demethylase KDM4B regulates otic vesicle invagination via epigenetic control of Dlx3 expression
title Histone demethylase KDM4B regulates otic vesicle invagination via epigenetic control of Dlx3 expression
spellingShingle Histone demethylase KDM4B regulates otic vesicle invagination via epigenetic control of Dlx3 expression
Uribe, Rosa A.
Epigenetics
Inner ear
Invagination
Dlx3
title_short Histone demethylase KDM4B regulates otic vesicle invagination via epigenetic control of Dlx3 expression
title_full Histone demethylase KDM4B regulates otic vesicle invagination via epigenetic control of Dlx3 expression
title_fullStr Histone demethylase KDM4B regulates otic vesicle invagination via epigenetic control of Dlx3 expression
title_full_unstemmed Histone demethylase KDM4B regulates otic vesicle invagination via epigenetic control of Dlx3 expression
title_sort Histone demethylase KDM4B regulates otic vesicle invagination via epigenetic control of Dlx3 expression
dc.creator.none.fl_str_mv Uribe, Rosa A.
Buzzi, Ailín Leticia
Bronner, Marianne E.
Strobl Mazulla, Pablo Hernan
author Uribe, Rosa A.
author_facet Uribe, Rosa A.
Buzzi, Ailín Leticia
Bronner, Marianne E.
Strobl Mazulla, Pablo Hernan
author_role author
author2 Buzzi, Ailín Leticia
Bronner, Marianne E.
Strobl Mazulla, Pablo Hernan
author2_role author
author
author
dc.subject.none.fl_str_mv Epigenetics
Inner ear
Invagination
Dlx3
topic Epigenetics
Inner ear
Invagination
Dlx3
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In vertebrates, the inner ear arises from the otic placode, a thickened swathe of ectoderm that invaginates to form the otic vesicle. We report that histone demethylase KDM4B is dynamically expressed during early stages of chick inner ear formation. A loss of KDM4B results in defective invagination and striking morphological changes in the otic epithelium, characterized by abnormal localization of adhesion and cytoskeletal molecules and reduced expression of several inner ear markers, including Dlx3. In vivo chromatin immunoprecipitation reveals direct and dynamic occupancy of KDM4B and its target, H3K9me3, at regulatory regions of the Dlx3 locus. Accordingly, coelectroporations of DLX3 or KDM4B encoding constructs, but not a catalytically dead mutant of KDM4B, rescue the ear invagination phenotype caused by KDM4B knockdown. Moreover, a loss of DLX3 phenocopies a loss of KDM4B. Collectively, our findings suggest that KDM4B play a critical role during inner ear invagination via modulating histone methylation of the direct target Dlx3.
Fil: Uribe, Rosa A.. California Institute of Technology; Estados Unidos
Fil: Buzzi, Ailín Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Fil: Bronner, Marianne E.. California Institute of Technology; Estados Unidos
Fil: Strobl Mazulla, Pablo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
description In vertebrates, the inner ear arises from the otic placode, a thickened swathe of ectoderm that invaginates to form the otic vesicle. We report that histone demethylase KDM4B is dynamically expressed during early stages of chick inner ear formation. A loss of KDM4B results in defective invagination and striking morphological changes in the otic epithelium, characterized by abnormal localization of adhesion and cytoskeletal molecules and reduced expression of several inner ear markers, including Dlx3. In vivo chromatin immunoprecipitation reveals direct and dynamic occupancy of KDM4B and its target, H3K9me3, at regulatory regions of the Dlx3 locus. Accordingly, coelectroporations of DLX3 or KDM4B encoding constructs, but not a catalytically dead mutant of KDM4B, rescue the ear invagination phenotype caused by KDM4B knockdown. Moreover, a loss of DLX3 phenocopies a loss of KDM4B. Collectively, our findings suggest that KDM4B play a critical role during inner ear invagination via modulating histone methylation of the direct target Dlx3.
publishDate 2015
dc.date.none.fl_str_mv 2015-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53679
Uribe, Rosa A.; Buzzi, Ailín Leticia; Bronner, Marianne E.; Strobl Mazulla, Pablo Hernan; Histone demethylase KDM4B regulates otic vesicle invagination via epigenetic control of Dlx3 expression; Rockefeller University Press; Journal of Cell Biology; 211; 4; 11-2015; 815-827
0021-9525
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53679
identifier_str_mv Uribe, Rosa A.; Buzzi, Ailín Leticia; Bronner, Marianne E.; Strobl Mazulla, Pablo Hernan; Histone demethylase KDM4B regulates otic vesicle invagination via epigenetic control of Dlx3 expression; Rockefeller University Press; Journal of Cell Biology; 211; 4; 11-2015; 815-827
0021-9525
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1083/jcb.201503071
info:eu-repo/semantics/altIdentifier/url/http://jcb.rupress.org/content/211/4/815
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Rockefeller University Press
publisher.none.fl_str_mv Rockefeller University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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