ATR-SEIRAs characterization of surface redox processes in G. sulfurreducens

Autores
Busalmen, Juan Pablo; Esteve Núñez, Abraham; Berná, Antonio; Feliu, Juan Miguel
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work we report on the occurrence of at least two different redox pairs on the cell surface of the electrogenic bacteria Geobacter sulfurreducens adsorbed on gold that are expressed in response to the polarization potential. As previously reported on graphite (Environ. Sci. Technol. 42 (2008) 2445) a typical low potential redox pair is found centered at around − 0.06 V when cells are polarized for a few hours at 0.2 V, while a new pair centered at around 0.38 V is expressed upon polarization at 0.6 V. Reversible changes in the IR band pattern of whole cells where obtained by Attenuated Total Reflection-Surface Enhanced Infrared Absorption Spectroscopy (ATR-SEIRAS) upon potential cycling around both redox pairs. Changes clearly resemble the electrochemical turnover of oxidized/reduced states in c-type cytochromes, thus evidencing the nature of the involved molecules. The expression of external cytochromes in response to the potential of the electron acceptor suggests the existence of alternative pathways of electron transport with different energy yield, though it remains to be demonstrated.
Fil: Busalmen, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigación en Ciencia y Tecnología de Materiales (i); Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina. Universidad de Alicante; España
Fil: Esteve Núñez, Abraham. Centro de Astrobiología; España
Fil: Berná, Antonio. Universidad de Alicante; España
Fil: Feliu, Juan Miguel. Universidad de Alicante; España
Materia
Electrode-Reducing Bacteria
Electron Transport Mechanism
Cytochromes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13242
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling ATR-SEIRAs characterization of surface redox processes in G. sulfurreducensBusalmen, Juan PabloEsteve Núñez, AbrahamBerná, AntonioFeliu, Juan MiguelElectrode-Reducing BacteriaElectron Transport MechanismCytochromeshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In this work we report on the occurrence of at least two different redox pairs on the cell surface of the electrogenic bacteria Geobacter sulfurreducens adsorbed on gold that are expressed in response to the polarization potential. As previously reported on graphite (Environ. Sci. Technol. 42 (2008) 2445) a typical low potential redox pair is found centered at around − 0.06 V when cells are polarized for a few hours at 0.2 V, while a new pair centered at around 0.38 V is expressed upon polarization at 0.6 V. Reversible changes in the IR band pattern of whole cells where obtained by Attenuated Total Reflection-Surface Enhanced Infrared Absorption Spectroscopy (ATR-SEIRAS) upon potential cycling around both redox pairs. Changes clearly resemble the electrochemical turnover of oxidized/reduced states in c-type cytochromes, thus evidencing the nature of the involved molecules. The expression of external cytochromes in response to the potential of the electron acceptor suggests the existence of alternative pathways of electron transport with different energy yield, though it remains to be demonstrated.Fil: Busalmen, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigación en Ciencia y Tecnología de Materiales (i); Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina. Universidad de Alicante; EspañaFil: Esteve Núñez, Abraham. Centro de Astrobiología; EspañaFil: Berná, Antonio. Universidad de Alicante; EspañaFil: Feliu, Juan Miguel. Universidad de Alicante; EspañaElsevier2009-05-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13242Busalmen, Juan Pablo; Esteve Núñez, Abraham; Berná, Antonio; Feliu, Juan Miguel; ATR-SEIRAs characterization of surface redox processes in G. sulfurreducens; Elsevier; Bioelectrochemistry; 78; 1; 9-5-2009; 25-291567-5394enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1567539409000863info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bioelechem.2009.04.011info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:01Zoai:ri.conicet.gov.ar:11336/13242instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:01.372CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv ATR-SEIRAs characterization of surface redox processes in G. sulfurreducens
title ATR-SEIRAs characterization of surface redox processes in G. sulfurreducens
spellingShingle ATR-SEIRAs characterization of surface redox processes in G. sulfurreducens
Busalmen, Juan Pablo
Electrode-Reducing Bacteria
Electron Transport Mechanism
Cytochromes
title_short ATR-SEIRAs characterization of surface redox processes in G. sulfurreducens
title_full ATR-SEIRAs characterization of surface redox processes in G. sulfurreducens
title_fullStr ATR-SEIRAs characterization of surface redox processes in G. sulfurreducens
title_full_unstemmed ATR-SEIRAs characterization of surface redox processes in G. sulfurreducens
title_sort ATR-SEIRAs characterization of surface redox processes in G. sulfurreducens
dc.creator.none.fl_str_mv Busalmen, Juan Pablo
Esteve Núñez, Abraham
Berná, Antonio
Feliu, Juan Miguel
author Busalmen, Juan Pablo
author_facet Busalmen, Juan Pablo
Esteve Núñez, Abraham
Berná, Antonio
Feliu, Juan Miguel
author_role author
author2 Esteve Núñez, Abraham
Berná, Antonio
Feliu, Juan Miguel
author2_role author
author
author
dc.subject.none.fl_str_mv Electrode-Reducing Bacteria
Electron Transport Mechanism
Cytochromes
topic Electrode-Reducing Bacteria
Electron Transport Mechanism
Cytochromes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this work we report on the occurrence of at least two different redox pairs on the cell surface of the electrogenic bacteria Geobacter sulfurreducens adsorbed on gold that are expressed in response to the polarization potential. As previously reported on graphite (Environ. Sci. Technol. 42 (2008) 2445) a typical low potential redox pair is found centered at around − 0.06 V when cells are polarized for a few hours at 0.2 V, while a new pair centered at around 0.38 V is expressed upon polarization at 0.6 V. Reversible changes in the IR band pattern of whole cells where obtained by Attenuated Total Reflection-Surface Enhanced Infrared Absorption Spectroscopy (ATR-SEIRAS) upon potential cycling around both redox pairs. Changes clearly resemble the electrochemical turnover of oxidized/reduced states in c-type cytochromes, thus evidencing the nature of the involved molecules. The expression of external cytochromes in response to the potential of the electron acceptor suggests the existence of alternative pathways of electron transport with different energy yield, though it remains to be demonstrated.
Fil: Busalmen, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigación en Ciencia y Tecnología de Materiales (i); Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina. Universidad de Alicante; España
Fil: Esteve Núñez, Abraham. Centro de Astrobiología; España
Fil: Berná, Antonio. Universidad de Alicante; España
Fil: Feliu, Juan Miguel. Universidad de Alicante; España
description In this work we report on the occurrence of at least two different redox pairs on the cell surface of the electrogenic bacteria Geobacter sulfurreducens adsorbed on gold that are expressed in response to the polarization potential. As previously reported on graphite (Environ. Sci. Technol. 42 (2008) 2445) a typical low potential redox pair is found centered at around − 0.06 V when cells are polarized for a few hours at 0.2 V, while a new pair centered at around 0.38 V is expressed upon polarization at 0.6 V. Reversible changes in the IR band pattern of whole cells where obtained by Attenuated Total Reflection-Surface Enhanced Infrared Absorption Spectroscopy (ATR-SEIRAS) upon potential cycling around both redox pairs. Changes clearly resemble the electrochemical turnover of oxidized/reduced states in c-type cytochromes, thus evidencing the nature of the involved molecules. The expression of external cytochromes in response to the potential of the electron acceptor suggests the existence of alternative pathways of electron transport with different energy yield, though it remains to be demonstrated.
publishDate 2009
dc.date.none.fl_str_mv 2009-05-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13242
Busalmen, Juan Pablo; Esteve Núñez, Abraham; Berná, Antonio; Feliu, Juan Miguel; ATR-SEIRAs characterization of surface redox processes in G. sulfurreducens; Elsevier; Bioelectrochemistry; 78; 1; 9-5-2009; 25-29
1567-5394
url http://hdl.handle.net/11336/13242
identifier_str_mv Busalmen, Juan Pablo; Esteve Núñez, Abraham; Berná, Antonio; Feliu, Juan Miguel; ATR-SEIRAs characterization of surface redox processes in G. sulfurreducens; Elsevier; Bioelectrochemistry; 78; 1; 9-5-2009; 25-29
1567-5394
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1567539409000863
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bioelechem.2009.04.011
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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