Enzymatic unhairing: Permeability assay of bovine skin epidermis with fungal enzyme extracts
- Autores
- Galarza, Betina Claudia; Garro, María Laura; Greco, Carlos A.; Hours, Roque Alberto
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The aim of enzymatic unhairing is to allow the separation of the outer sheath of hair root from the dermal connective tissue. This is considered an ecofriendly process. It?s not expensive since if the enzymes are applied on the epidermis thereby surface extracts could be saved. The purpose of this work was to analyze the changes in pilosebaceus unit and basal lamina by unhairing enzyme action. Various keratinolytic fungi, previously isolated and selected from different soil samples were used: Neurospora crassa, Verticillum albo-atrum, Trichophyton ajelloi, Chrysosporium sp, Aspergillus sydowii, Paecilomyces lilacinus and Acremonium murorum. Fungal isolates were cultivated in solid state conditions using hair waste obtained from the hair-saving unhairing process as substrate. Enzyme extracts were characterized according to their keratinolytic-proteolytic activity and protein content. Bovine skins were placed in plastic plates where the epidermis contacted with different solutions: fungal enzyme extracts with commercial tensioactives (0.1%, 0.5%), biocide 0.2% w/w (wet skin) for 4 h (soaking) and 24 h (unhairing) at 25°C and 40 rpm. Samples of skin with and without enzymatic treatment were fixed in 4% formaldehyde, dried on increasing concentration of ethanol and analyzed with scanning electron microscopy. It was observed that the enzymes altered the epidermis, caused detachment of corneocytes and the emptying of hair follicles. Characteristic patterns of hair cuticle and collagen fibres were preserved in skin control with buffer. Trichophyton ajelloi caused the strongest depilatory effect . It was concluded that the enzyme penetration through epidermis could be employed for the application of reagents during the handcrafted tanning of leather.
Fil: Galarza, Betina Claudia. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo del Cuero. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Centíficas. Centro de Investigación y Desarrollo del Cuero; Argentina
Fil: Garro, María Laura. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo del Cuero. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Centíficas. Centro de Investigación y Desarrollo del Cuero; Argentina
Fil: Greco, Carlos A.. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo del Cuero. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Centíficas. Centro de Investigación y Desarrollo del Cuero; Argentina
Fil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina - Materia
-
UNHAIRING
KERATINOLYTIC FUNGAL ENZYMES
PERMEABILITY
EPIDERMIS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/122652
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CONICET Digital (CONICET) |
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Enzymatic unhairing: Permeability assay of bovine skin epidermis with fungal enzyme extractsGalarza, Betina ClaudiaGarro, María LauraGreco, Carlos A.Hours, Roque AlbertoUNHAIRINGKERATINOLYTIC FUNGAL ENZYMESPERMEABILITYEPIDERMIShttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The aim of enzymatic unhairing is to allow the separation of the outer sheath of hair root from the dermal connective tissue. This is considered an ecofriendly process. It?s not expensive since if the enzymes are applied on the epidermis thereby surface extracts could be saved. The purpose of this work was to analyze the changes in pilosebaceus unit and basal lamina by unhairing enzyme action. Various keratinolytic fungi, previously isolated and selected from different soil samples were used: Neurospora crassa, Verticillum albo-atrum, Trichophyton ajelloi, Chrysosporium sp, Aspergillus sydowii, Paecilomyces lilacinus and Acremonium murorum. Fungal isolates were cultivated in solid state conditions using hair waste obtained from the hair-saving unhairing process as substrate. Enzyme extracts were characterized according to their keratinolytic-proteolytic activity and protein content. Bovine skins were placed in plastic plates where the epidermis contacted with different solutions: fungal enzyme extracts with commercial tensioactives (0.1%, 0.5%), biocide 0.2% w/w (wet skin) for 4 h (soaking) and 24 h (unhairing) at 25°C and 40 rpm. Samples of skin with and without enzymatic treatment were fixed in 4% formaldehyde, dried on increasing concentration of ethanol and analyzed with scanning electron microscopy. It was observed that the enzymes altered the epidermis, caused detachment of corneocytes and the emptying of hair follicles. Characteristic patterns of hair cuticle and collagen fibres were preserved in skin control with buffer. Trichophyton ajelloi caused the strongest depilatory effect . It was concluded that the enzyme penetration through epidermis could be employed for the application of reagents during the handcrafted tanning of leather.Fil: Galarza, Betina Claudia. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo del Cuero. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Centíficas. Centro de Investigación y Desarrollo del Cuero; ArgentinaFil: Garro, María Laura. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo del Cuero. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Centíficas. Centro de Investigación y Desarrollo del Cuero; ArgentinaFil: Greco, Carlos A.. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo del Cuero. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Centíficas. Centro de Investigación y Desarrollo del Cuero; ArgentinaFil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaAsociación Química Española de la Industria del Cuero2019-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/122652Galarza, Betina Claudia; Garro, María Laura; Greco, Carlos A.; Hours, Roque Alberto; Enzymatic unhairing: Permeability assay of bovine skin epidermis with fungal enzyme extracts; Asociación Química Española de la Industria del Cuero; Journal of AQEIC; 70; 1; 7-2019; 15-212014-8011CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.aqeic.org/web/journal/esp/index.phpinfo:eu-repo/semantics/altIdentifier/url/https://dialnet.unirioja.es/servlet/articulo?codigo=7058914info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:30:50Zoai:ri.conicet.gov.ar:11336/122652instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:30:51.245CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Enzymatic unhairing: Permeability assay of bovine skin epidermis with fungal enzyme extracts |
title |
Enzymatic unhairing: Permeability assay of bovine skin epidermis with fungal enzyme extracts |
spellingShingle |
Enzymatic unhairing: Permeability assay of bovine skin epidermis with fungal enzyme extracts Galarza, Betina Claudia UNHAIRING KERATINOLYTIC FUNGAL ENZYMES PERMEABILITY EPIDERMIS |
title_short |
Enzymatic unhairing: Permeability assay of bovine skin epidermis with fungal enzyme extracts |
title_full |
Enzymatic unhairing: Permeability assay of bovine skin epidermis with fungal enzyme extracts |
title_fullStr |
Enzymatic unhairing: Permeability assay of bovine skin epidermis with fungal enzyme extracts |
title_full_unstemmed |
Enzymatic unhairing: Permeability assay of bovine skin epidermis with fungal enzyme extracts |
title_sort |
Enzymatic unhairing: Permeability assay of bovine skin epidermis with fungal enzyme extracts |
dc.creator.none.fl_str_mv |
Galarza, Betina Claudia Garro, María Laura Greco, Carlos A. Hours, Roque Alberto |
author |
Galarza, Betina Claudia |
author_facet |
Galarza, Betina Claudia Garro, María Laura Greco, Carlos A. Hours, Roque Alberto |
author_role |
author |
author2 |
Garro, María Laura Greco, Carlos A. Hours, Roque Alberto |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
UNHAIRING KERATINOLYTIC FUNGAL ENZYMES PERMEABILITY EPIDERMIS |
topic |
UNHAIRING KERATINOLYTIC FUNGAL ENZYMES PERMEABILITY EPIDERMIS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
The aim of enzymatic unhairing is to allow the separation of the outer sheath of hair root from the dermal connective tissue. This is considered an ecofriendly process. It?s not expensive since if the enzymes are applied on the epidermis thereby surface extracts could be saved. The purpose of this work was to analyze the changes in pilosebaceus unit and basal lamina by unhairing enzyme action. Various keratinolytic fungi, previously isolated and selected from different soil samples were used: Neurospora crassa, Verticillum albo-atrum, Trichophyton ajelloi, Chrysosporium sp, Aspergillus sydowii, Paecilomyces lilacinus and Acremonium murorum. Fungal isolates were cultivated in solid state conditions using hair waste obtained from the hair-saving unhairing process as substrate. Enzyme extracts were characterized according to their keratinolytic-proteolytic activity and protein content. Bovine skins were placed in plastic plates where the epidermis contacted with different solutions: fungal enzyme extracts with commercial tensioactives (0.1%, 0.5%), biocide 0.2% w/w (wet skin) for 4 h (soaking) and 24 h (unhairing) at 25°C and 40 rpm. Samples of skin with and without enzymatic treatment were fixed in 4% formaldehyde, dried on increasing concentration of ethanol and analyzed with scanning electron microscopy. It was observed that the enzymes altered the epidermis, caused detachment of corneocytes and the emptying of hair follicles. Characteristic patterns of hair cuticle and collagen fibres were preserved in skin control with buffer. Trichophyton ajelloi caused the strongest depilatory effect . It was concluded that the enzyme penetration through epidermis could be employed for the application of reagents during the handcrafted tanning of leather. Fil: Galarza, Betina Claudia. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo del Cuero. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Centíficas. Centro de Investigación y Desarrollo del Cuero; Argentina Fil: Garro, María Laura. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo del Cuero. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Centíficas. Centro de Investigación y Desarrollo del Cuero; Argentina Fil: Greco, Carlos A.. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo del Cuero. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Centíficas. Centro de Investigación y Desarrollo del Cuero; Argentina Fil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina |
description |
The aim of enzymatic unhairing is to allow the separation of the outer sheath of hair root from the dermal connective tissue. This is considered an ecofriendly process. It?s not expensive since if the enzymes are applied on the epidermis thereby surface extracts could be saved. The purpose of this work was to analyze the changes in pilosebaceus unit and basal lamina by unhairing enzyme action. Various keratinolytic fungi, previously isolated and selected from different soil samples were used: Neurospora crassa, Verticillum albo-atrum, Trichophyton ajelloi, Chrysosporium sp, Aspergillus sydowii, Paecilomyces lilacinus and Acremonium murorum. Fungal isolates were cultivated in solid state conditions using hair waste obtained from the hair-saving unhairing process as substrate. Enzyme extracts were characterized according to their keratinolytic-proteolytic activity and protein content. Bovine skins were placed in plastic plates where the epidermis contacted with different solutions: fungal enzyme extracts with commercial tensioactives (0.1%, 0.5%), biocide 0.2% w/w (wet skin) for 4 h (soaking) and 24 h (unhairing) at 25°C and 40 rpm. Samples of skin with and without enzymatic treatment were fixed in 4% formaldehyde, dried on increasing concentration of ethanol and analyzed with scanning electron microscopy. It was observed that the enzymes altered the epidermis, caused detachment of corneocytes and the emptying of hair follicles. Characteristic patterns of hair cuticle and collagen fibres were preserved in skin control with buffer. Trichophyton ajelloi caused the strongest depilatory effect . It was concluded that the enzyme penetration through epidermis could be employed for the application of reagents during the handcrafted tanning of leather. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/122652 Galarza, Betina Claudia; Garro, María Laura; Greco, Carlos A.; Hours, Roque Alberto; Enzymatic unhairing: Permeability assay of bovine skin epidermis with fungal enzyme extracts; Asociación Química Española de la Industria del Cuero; Journal of AQEIC; 70; 1; 7-2019; 15-21 2014-8011 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/122652 |
identifier_str_mv |
Galarza, Betina Claudia; Garro, María Laura; Greco, Carlos A.; Hours, Roque Alberto; Enzymatic unhairing: Permeability assay of bovine skin epidermis with fungal enzyme extracts; Asociación Química Española de la Industria del Cuero; Journal of AQEIC; 70; 1; 7-2019; 15-21 2014-8011 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.aqeic.org/web/journal/esp/index.php info:eu-repo/semantics/altIdentifier/url/https://dialnet.unirioja.es/servlet/articulo?codigo=7058914 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Asociación Química Española de la Industria del Cuero |
publisher.none.fl_str_mv |
Asociación Química Española de la Industria del Cuero |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846082787755425792 |
score |
13.22299 |