Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants
- Autores
- Ullio Gamboa, Gabriela Veronica; Benedini, Luciano Alejandro; Schulz, Pablo Carlos; Allemandi, Daniel Alberto
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In this work, the phase behavior variations of an ascorbyl palmitate (Asc16) system in aqueous solution were analyzed when immunologically active hydrophilic compounds (CpG and OVA) were introduced. This study was carried out through optical polarizing microscopy (OPM) and differential scanning calorimetry (DSC) at different temperatures and over a broad range of concentrations. The combination of both techniques allowed the determination of a complete phase diagram which was compared with those built for Asc16-water system and it was demonstrated that fixed concentrations of hydrophilic compounds (300 and 24 µg/g for CpG-ODN and OVA respectively) generate two lamellar liquid crystals, a cubic liquid crystal phase, and also other aggregates. However, no changes were observed in the phase diagram in terms of formation of new mesophases. The aqueous phase behavior was also studied as a function of surfactant and temperature. DSC and Fourier transform infrared spectroscopy (FT-IR) measurements show differences in the free water and mainly in the secondary hydration layer, which confirm that the studied compounds are situated in the aqueous domain. The construction and analysis of Asc16 phase diagrams with a fixed concentration of CpG-ODN/OVA allows the comprehension of Asc16 phase behavior and could be easily adapted to other concentrations. Moreover, these findings could be extrapolated to other hydrophilic substances in aqueous solution introduced in liquid crystal phases since they follow a similar behavior as those reported in the literature.
Fil: Ullio Gamboa, Gabriela Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Fil: Benedini, Luciano Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Schulz, Pablo Carlos. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Allemandi, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina - Materia
-
Ascorbyl Palmitate
Liquid Crystals
Oligonucleotides
Phase Diagrams
Water Behavior - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/54782
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Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine AdjuvantsUllio Gamboa, Gabriela VeronicaBenedini, Luciano AlejandroSchulz, Pablo CarlosAllemandi, Daniel AlbertoAscorbyl PalmitateLiquid CrystalsOligonucleotidesPhase DiagramsWater Behaviorhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In this work, the phase behavior variations of an ascorbyl palmitate (Asc16) system in aqueous solution were analyzed when immunologically active hydrophilic compounds (CpG and OVA) were introduced. This study was carried out through optical polarizing microscopy (OPM) and differential scanning calorimetry (DSC) at different temperatures and over a broad range of concentrations. The combination of both techniques allowed the determination of a complete phase diagram which was compared with those built for Asc16-water system and it was demonstrated that fixed concentrations of hydrophilic compounds (300 and 24 µg/g for CpG-ODN and OVA respectively) generate two lamellar liquid crystals, a cubic liquid crystal phase, and also other aggregates. However, no changes were observed in the phase diagram in terms of formation of new mesophases. The aqueous phase behavior was also studied as a function of surfactant and temperature. DSC and Fourier transform infrared spectroscopy (FT-IR) measurements show differences in the free water and mainly in the secondary hydration layer, which confirm that the studied compounds are situated in the aqueous domain. The construction and analysis of Asc16 phase diagrams with a fixed concentration of CpG-ODN/OVA allows the comprehension of Asc16 phase behavior and could be easily adapted to other concentrations. Moreover, these findings could be extrapolated to other hydrophilic substances in aqueous solution introduced in liquid crystal phases since they follow a similar behavior as those reported in the literature.Fil: Ullio Gamboa, Gabriela Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaFil: Benedini, Luciano Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Schulz, Pablo Carlos. Universidad Nacional del Sur. Departamento de Química; ArgentinaFil: Allemandi, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaSpringer Heidelberg2016-07-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54782Ullio Gamboa, Gabriela Veronica; Benedini, Luciano Alejandro; Schulz, Pablo Carlos; Allemandi, Daniel Alberto; Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants; Springer Heidelberg; Journal Of Surfactants And Detergents; 19; 4; 26-7-2016; 747-7571097-3958CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1007/s11743-016-1816-9info:eu-repo/semantics/altIdentifier/doi/10.1007/s11743-016-1816-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:30Zoai:ri.conicet.gov.ar:11336/54782instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:30.48CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants |
title |
Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants |
spellingShingle |
Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants Ullio Gamboa, Gabriela Veronica Ascorbyl Palmitate Liquid Crystals Oligonucleotides Phase Diagrams Water Behavior |
title_short |
Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants |
title_full |
Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants |
title_fullStr |
Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants |
title_full_unstemmed |
Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants |
title_sort |
Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants |
dc.creator.none.fl_str_mv |
Ullio Gamboa, Gabriela Veronica Benedini, Luciano Alejandro Schulz, Pablo Carlos Allemandi, Daniel Alberto |
author |
Ullio Gamboa, Gabriela Veronica |
author_facet |
Ullio Gamboa, Gabriela Veronica Benedini, Luciano Alejandro Schulz, Pablo Carlos Allemandi, Daniel Alberto |
author_role |
author |
author2 |
Benedini, Luciano Alejandro Schulz, Pablo Carlos Allemandi, Daniel Alberto |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Ascorbyl Palmitate Liquid Crystals Oligonucleotides Phase Diagrams Water Behavior |
topic |
Ascorbyl Palmitate Liquid Crystals Oligonucleotides Phase Diagrams Water Behavior |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
In this work, the phase behavior variations of an ascorbyl palmitate (Asc16) system in aqueous solution were analyzed when immunologically active hydrophilic compounds (CpG and OVA) were introduced. This study was carried out through optical polarizing microscopy (OPM) and differential scanning calorimetry (DSC) at different temperatures and over a broad range of concentrations. The combination of both techniques allowed the determination of a complete phase diagram which was compared with those built for Asc16-water system and it was demonstrated that fixed concentrations of hydrophilic compounds (300 and 24 µg/g for CpG-ODN and OVA respectively) generate two lamellar liquid crystals, a cubic liquid crystal phase, and also other aggregates. However, no changes were observed in the phase diagram in terms of formation of new mesophases. The aqueous phase behavior was also studied as a function of surfactant and temperature. DSC and Fourier transform infrared spectroscopy (FT-IR) measurements show differences in the free water and mainly in the secondary hydration layer, which confirm that the studied compounds are situated in the aqueous domain. The construction and analysis of Asc16 phase diagrams with a fixed concentration of CpG-ODN/OVA allows the comprehension of Asc16 phase behavior and could be easily adapted to other concentrations. Moreover, these findings could be extrapolated to other hydrophilic substances in aqueous solution introduced in liquid crystal phases since they follow a similar behavior as those reported in the literature. Fil: Ullio Gamboa, Gabriela Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina Fil: Benedini, Luciano Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina Fil: Schulz, Pablo Carlos. Universidad Nacional del Sur. Departamento de Química; Argentina Fil: Allemandi, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina |
description |
In this work, the phase behavior variations of an ascorbyl palmitate (Asc16) system in aqueous solution were analyzed when immunologically active hydrophilic compounds (CpG and OVA) were introduced. This study was carried out through optical polarizing microscopy (OPM) and differential scanning calorimetry (DSC) at different temperatures and over a broad range of concentrations. The combination of both techniques allowed the determination of a complete phase diagram which was compared with those built for Asc16-water system and it was demonstrated that fixed concentrations of hydrophilic compounds (300 and 24 µg/g for CpG-ODN and OVA respectively) generate two lamellar liquid crystals, a cubic liquid crystal phase, and also other aggregates. However, no changes were observed in the phase diagram in terms of formation of new mesophases. The aqueous phase behavior was also studied as a function of surfactant and temperature. DSC and Fourier transform infrared spectroscopy (FT-IR) measurements show differences in the free water and mainly in the secondary hydration layer, which confirm that the studied compounds are situated in the aqueous domain. The construction and analysis of Asc16 phase diagrams with a fixed concentration of CpG-ODN/OVA allows the comprehension of Asc16 phase behavior and could be easily adapted to other concentrations. Moreover, these findings could be extrapolated to other hydrophilic substances in aqueous solution introduced in liquid crystal phases since they follow a similar behavior as those reported in the literature. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-07-26 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/54782 Ullio Gamboa, Gabriela Veronica; Benedini, Luciano Alejandro; Schulz, Pablo Carlos; Allemandi, Daniel Alberto; Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants; Springer Heidelberg; Journal Of Surfactants And Detergents; 19; 4; 26-7-2016; 747-757 1097-3958 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/54782 |
identifier_str_mv |
Ullio Gamboa, Gabriela Veronica; Benedini, Luciano Alejandro; Schulz, Pablo Carlos; Allemandi, Daniel Alberto; Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants; Springer Heidelberg; Journal Of Surfactants And Detergents; 19; 4; 26-7-2016; 747-757 1097-3958 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1007/s11743-016-1816-9 info:eu-repo/semantics/altIdentifier/doi/10.1007/s11743-016-1816-9 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Springer Heidelberg |
publisher.none.fl_str_mv |
Springer Heidelberg |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613610088693760 |
score |
13.070432 |