Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants

Autores
Ullio Gamboa, Gabriela Veronica; Benedini, Luciano Alejandro; Schulz, Pablo Carlos; Allemandi, Daniel Alberto
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work, the phase behavior variations of an ascorbyl palmitate (Asc16) system in aqueous solution were analyzed when immunologically active hydrophilic compounds (CpG and OVA) were introduced. This study was carried out through optical polarizing microscopy (OPM) and differential scanning calorimetry (DSC) at different temperatures and over a broad range of concentrations. The combination of both techniques allowed the determination of a complete phase diagram which was compared with those built for Asc16-water system and it was demonstrated that fixed concentrations of hydrophilic compounds (300 and 24 µg/g for CpG-ODN and OVA respectively) generate two lamellar liquid crystals, a cubic liquid crystal phase, and also other aggregates. However, no changes were observed in the phase diagram in terms of formation of new mesophases. The aqueous phase behavior was also studied as a function of surfactant and temperature. DSC and Fourier transform infrared spectroscopy (FT-IR) measurements show differences in the free water and mainly in the secondary hydration layer, which confirm that the studied compounds are situated in the aqueous domain. The construction and analysis of Asc16 phase diagrams with a fixed concentration of CpG-ODN/OVA allows the comprehension of Asc16 phase behavior and could be easily adapted to other concentrations. Moreover, these findings could be extrapolated to other hydrophilic substances in aqueous solution introduced in liquid crystal phases since they follow a similar behavior as those reported in the literature.
Fil: Ullio Gamboa, Gabriela Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Fil: Benedini, Luciano Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Schulz, Pablo Carlos. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Allemandi, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Materia
Ascorbyl Palmitate
Liquid Crystals
Oligonucleotides
Phase Diagrams
Water Behavior
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54782
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/54782
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine AdjuvantsUllio Gamboa, Gabriela VeronicaBenedini, Luciano AlejandroSchulz, Pablo CarlosAllemandi, Daniel AlbertoAscorbyl PalmitateLiquid CrystalsOligonucleotidesPhase DiagramsWater Behaviorhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In this work, the phase behavior variations of an ascorbyl palmitate (Asc16) system in aqueous solution were analyzed when immunologically active hydrophilic compounds (CpG and OVA) were introduced. This study was carried out through optical polarizing microscopy (OPM) and differential scanning calorimetry (DSC) at different temperatures and over a broad range of concentrations. The combination of both techniques allowed the determination of a complete phase diagram which was compared with those built for Asc16-water system and it was demonstrated that fixed concentrations of hydrophilic compounds (300 and 24 µg/g for CpG-ODN and OVA respectively) generate two lamellar liquid crystals, a cubic liquid crystal phase, and also other aggregates. However, no changes were observed in the phase diagram in terms of formation of new mesophases. The aqueous phase behavior was also studied as a function of surfactant and temperature. DSC and Fourier transform infrared spectroscopy (FT-IR) measurements show differences in the free water and mainly in the secondary hydration layer, which confirm that the studied compounds are situated in the aqueous domain. The construction and analysis of Asc16 phase diagrams with a fixed concentration of CpG-ODN/OVA allows the comprehension of Asc16 phase behavior and could be easily adapted to other concentrations. Moreover, these findings could be extrapolated to other hydrophilic substances in aqueous solution introduced in liquid crystal phases since they follow a similar behavior as those reported in the literature.Fil: Ullio Gamboa, Gabriela Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaFil: Benedini, Luciano Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Schulz, Pablo Carlos. Universidad Nacional del Sur. Departamento de Química; ArgentinaFil: Allemandi, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaSpringer Heidelberg2016-07-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54782Ullio Gamboa, Gabriela Veronica; Benedini, Luciano Alejandro; Schulz, Pablo Carlos; Allemandi, Daniel Alberto; Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants; Springer Heidelberg; Journal Of Surfactants And Detergents; 19; 4; 26-7-2016; 747-7571097-3958CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1007/s11743-016-1816-9info:eu-repo/semantics/altIdentifier/doi/10.1007/s11743-016-1816-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:47:24Zoai:ri.conicet.gov.ar:11336/54782instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:47:24.921CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants
title Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants
spellingShingle Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants
Ullio Gamboa, Gabriela Veronica
Ascorbyl Palmitate
Liquid Crystals
Oligonucleotides
Phase Diagrams
Water Behavior
title_short Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants
title_full Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants
title_fullStr Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants
title_full_unstemmed Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants
title_sort Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants
dc.creator.none.fl_str_mv Ullio Gamboa, Gabriela Veronica
Benedini, Luciano Alejandro
Schulz, Pablo Carlos
Allemandi, Daniel Alberto
author Ullio Gamboa, Gabriela Veronica
author_facet Ullio Gamboa, Gabriela Veronica
Benedini, Luciano Alejandro
Schulz, Pablo Carlos
Allemandi, Daniel Alberto
author_role author
author2 Benedini, Luciano Alejandro
Schulz, Pablo Carlos
Allemandi, Daniel Alberto
author2_role author
author
author
dc.subject.none.fl_str_mv Ascorbyl Palmitate
Liquid Crystals
Oligonucleotides
Phase Diagrams
Water Behavior
topic Ascorbyl Palmitate
Liquid Crystals
Oligonucleotides
Phase Diagrams
Water Behavior
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this work, the phase behavior variations of an ascorbyl palmitate (Asc16) system in aqueous solution were analyzed when immunologically active hydrophilic compounds (CpG and OVA) were introduced. This study was carried out through optical polarizing microscopy (OPM) and differential scanning calorimetry (DSC) at different temperatures and over a broad range of concentrations. The combination of both techniques allowed the determination of a complete phase diagram which was compared with those built for Asc16-water system and it was demonstrated that fixed concentrations of hydrophilic compounds (300 and 24 µg/g for CpG-ODN and OVA respectively) generate two lamellar liquid crystals, a cubic liquid crystal phase, and also other aggregates. However, no changes were observed in the phase diagram in terms of formation of new mesophases. The aqueous phase behavior was also studied as a function of surfactant and temperature. DSC and Fourier transform infrared spectroscopy (FT-IR) measurements show differences in the free water and mainly in the secondary hydration layer, which confirm that the studied compounds are situated in the aqueous domain. The construction and analysis of Asc16 phase diagrams with a fixed concentration of CpG-ODN/OVA allows the comprehension of Asc16 phase behavior and could be easily adapted to other concentrations. Moreover, these findings could be extrapolated to other hydrophilic substances in aqueous solution introduced in liquid crystal phases since they follow a similar behavior as those reported in the literature.
Fil: Ullio Gamboa, Gabriela Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Fil: Benedini, Luciano Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Schulz, Pablo Carlos. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Allemandi, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
description In this work, the phase behavior variations of an ascorbyl palmitate (Asc16) system in aqueous solution were analyzed when immunologically active hydrophilic compounds (CpG and OVA) were introduced. This study was carried out through optical polarizing microscopy (OPM) and differential scanning calorimetry (DSC) at different temperatures and over a broad range of concentrations. The combination of both techniques allowed the determination of a complete phase diagram which was compared with those built for Asc16-water system and it was demonstrated that fixed concentrations of hydrophilic compounds (300 and 24 µg/g for CpG-ODN and OVA respectively) generate two lamellar liquid crystals, a cubic liquid crystal phase, and also other aggregates. However, no changes were observed in the phase diagram in terms of formation of new mesophases. The aqueous phase behavior was also studied as a function of surfactant and temperature. DSC and Fourier transform infrared spectroscopy (FT-IR) measurements show differences in the free water and mainly in the secondary hydration layer, which confirm that the studied compounds are situated in the aqueous domain. The construction and analysis of Asc16 phase diagrams with a fixed concentration of CpG-ODN/OVA allows the comprehension of Asc16 phase behavior and could be easily adapted to other concentrations. Moreover, these findings could be extrapolated to other hydrophilic substances in aqueous solution introduced in liquid crystal phases since they follow a similar behavior as those reported in the literature.
publishDate 2016
dc.date.none.fl_str_mv 2016-07-26
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54782
Ullio Gamboa, Gabriela Veronica; Benedini, Luciano Alejandro; Schulz, Pablo Carlos; Allemandi, Daniel Alberto; Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants; Springer Heidelberg; Journal Of Surfactants And Detergents; 19; 4; 26-7-2016; 747-757
1097-3958
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54782
identifier_str_mv Ullio Gamboa, Gabriela Veronica; Benedini, Luciano Alejandro; Schulz, Pablo Carlos; Allemandi, Daniel Alberto; Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants; Springer Heidelberg; Journal Of Surfactants And Detergents; 19; 4; 26-7-2016; 747-757
1097-3958
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1007/s11743-016-1816-9
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11743-016-1816-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Heidelberg
publisher.none.fl_str_mv Springer Heidelberg
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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