Oral administration of a catalase-producing Lactococcus lactis can prevent a chemically induced colon cancer in mice

Autores
de Moreno, Maria Alejandra; Leblanc, Jean Guy Joseph; Perdigon, Gabriela del Valle; Miyoshi, Anderson; Langella, Philippe; Azevedo, Vaso; Sesma, Fernando Juan Manuel
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Reactive oxygen species, such as hydrogen peroxide (H2O 2), are involved in various aspects of tumour development. Decreasing their levels can therefore be a promising approach for colon cancer prevention. The objective of this study was to evaluate the effect of catalase-producing Lactococcus lactis on the prevention of an experimental murine 1,2-dimethylhydrazine (DMH)-induced colon cancer. DMH-treated BALB/c mice received either a catalase-producing L. lactis strain or the isogenic non-catalase-producing strain as a control, whereas other untreated mice did not receive bacterial supplementation. Catalase activity and H2O 2 levels in intestinal fluids and blood samples were measured, and changes in the histology of the large intestines during tumour progression were evaluated. The catalase-producing L. lactis strain used in this study was able to slightly increase catalase activities in DMH-treated mice (1.19 ± 0.08 U ml-1) and reduce H2O2 levels (3.4 ± 1.1 μM) compared to (i) animals that received the non-catalase-producing strain (1.00 ± 0.09 U ml-1, 9.0 ± 0.8 μM), and (ii) those that did not receive bacterial supplementation (1.06 ± 0.07 U ml-1, 10.0 ± 1.1 μM). Using the histopathological grading scale of chemically induced colorectal cancer, animals that received the catalase-producing L. lactis had a significantly lesser extent of colonic damage and inflammation (2.0 ± 0.4) compared to animals that received the non-catalase-producing L. lactis (4.0 ± 0.3) or those that did not receive bacterial supplementation (4.7 ± 0.5). The catalase-producing L. lactis strain used in this study was able to prevent tumour appearance in an experimental DMH-induced colon cancer model.
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Miyoshi, Anderson. Universidade Federal de Minas Gerais; Brasil
Fil: Langella, Philippe. Institut National de la Recherche Agronomique; Francia
Fil: Azevedo, Vaso. Universidade Federal de Minas Gerais; Brasil
Fil: Sesma, Fernando Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
Catalase
Lactic Acid Bacteria
Colon Cancer
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/56483
Acceder
id CONICETDig_f44e54fee7ad9ff647c9b42c38eeb491
oai_identifier_str oai:ri.conicet.gov.ar:11336/56483
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Oral administration of a catalase-producing Lactococcus lactis can prevent a chemically induced colon cancer in micede Moreno, Maria AlejandraLeblanc, Jean Guy JosephPerdigon, Gabriela del ValleMiyoshi, AndersonLangella, PhilippeAzevedo, VasoSesma, Fernando Juan ManuelCatalaseLactic Acid BacteriaColon Cancerhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Reactive oxygen species, such as hydrogen peroxide (H2O 2), are involved in various aspects of tumour development. Decreasing their levels can therefore be a promising approach for colon cancer prevention. The objective of this study was to evaluate the effect of catalase-producing Lactococcus lactis on the prevention of an experimental murine 1,2-dimethylhydrazine (DMH)-induced colon cancer. DMH-treated BALB/c mice received either a catalase-producing L. lactis strain or the isogenic non-catalase-producing strain as a control, whereas other untreated mice did not receive bacterial supplementation. Catalase activity and H2O 2 levels in intestinal fluids and blood samples were measured, and changes in the histology of the large intestines during tumour progression were evaluated. The catalase-producing L. lactis strain used in this study was able to slightly increase catalase activities in DMH-treated mice (1.19 ± 0.08 U ml-1) and reduce H2O2 levels (3.4 ± 1.1 μM) compared to (i) animals that received the non-catalase-producing strain (1.00 ± 0.09 U ml-1, 9.0 ± 0.8 μM), and (ii) those that did not receive bacterial supplementation (1.06 ± 0.07 U ml-1, 10.0 ± 1.1 μM). Using the histopathological grading scale of chemically induced colorectal cancer, animals that received the catalase-producing L. lactis had a significantly lesser extent of colonic damage and inflammation (2.0 ± 0.4) compared to animals that received the non-catalase-producing L. lactis (4.0 ± 0.3) or those that did not receive bacterial supplementation (4.7 ± 0.5). The catalase-producing L. lactis strain used in this study was able to prevent tumour appearance in an experimental DMH-induced colon cancer model.Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Miyoshi, Anderson. Universidade Federal de Minas Gerais; BrasilFil: Langella, Philippe. Institut National de la Recherche Agronomique; FranciaFil: Azevedo, Vaso. Universidade Federal de Minas Gerais; BrasilFil: Sesma, Fernando Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaSociety for General Microbiology2008-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/56483de Moreno, Maria Alejandra; Leblanc, Jean Guy Joseph; Perdigon, Gabriela del Valle; Miyoshi, Anderson; Langella, Philippe; et al.; Oral administration of a catalase-producing Lactococcus lactis can prevent a chemically induced colon cancer in mice; Society for General Microbiology; Journal Of Medical Microbiology; 57; 1; 1-2008; 100-1050022-26151473-5644CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1099/jmm.0.47403-0info:eu-repo/semantics/altIdentifier/url/http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.47403-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:33:21Zoai:ri.conicet.gov.ar:11336/56483instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:33:22.03CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Oral administration of a catalase-producing Lactococcus lactis can prevent a chemically induced colon cancer in mice
title Oral administration of a catalase-producing Lactococcus lactis can prevent a chemically induced colon cancer in mice
spellingShingle Oral administration of a catalase-producing Lactococcus lactis can prevent a chemically induced colon cancer in mice
de Moreno, Maria Alejandra
Catalase
Lactic Acid Bacteria
Colon Cancer
title_short Oral administration of a catalase-producing Lactococcus lactis can prevent a chemically induced colon cancer in mice
title_full Oral administration of a catalase-producing Lactococcus lactis can prevent a chemically induced colon cancer in mice
title_fullStr Oral administration of a catalase-producing Lactococcus lactis can prevent a chemically induced colon cancer in mice
title_full_unstemmed Oral administration of a catalase-producing Lactococcus lactis can prevent a chemically induced colon cancer in mice
title_sort Oral administration of a catalase-producing Lactococcus lactis can prevent a chemically induced colon cancer in mice
dc.creator.none.fl_str_mv de Moreno, Maria Alejandra
Leblanc, Jean Guy Joseph
Perdigon, Gabriela del Valle
Miyoshi, Anderson
Langella, Philippe
Azevedo, Vaso
Sesma, Fernando Juan Manuel
author de Moreno, Maria Alejandra
author_facet de Moreno, Maria Alejandra
Leblanc, Jean Guy Joseph
Perdigon, Gabriela del Valle
Miyoshi, Anderson
Langella, Philippe
Azevedo, Vaso
Sesma, Fernando Juan Manuel
author_role author
author2 Leblanc, Jean Guy Joseph
Perdigon, Gabriela del Valle
Miyoshi, Anderson
Langella, Philippe
Azevedo, Vaso
Sesma, Fernando Juan Manuel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Catalase
Lactic Acid Bacteria
Colon Cancer
topic Catalase
Lactic Acid Bacteria
Colon Cancer
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Reactive oxygen species, such as hydrogen peroxide (H2O 2), are involved in various aspects of tumour development. Decreasing their levels can therefore be a promising approach for colon cancer prevention. The objective of this study was to evaluate the effect of catalase-producing Lactococcus lactis on the prevention of an experimental murine 1,2-dimethylhydrazine (DMH)-induced colon cancer. DMH-treated BALB/c mice received either a catalase-producing L. lactis strain or the isogenic non-catalase-producing strain as a control, whereas other untreated mice did not receive bacterial supplementation. Catalase activity and H2O 2 levels in intestinal fluids and blood samples were measured, and changes in the histology of the large intestines during tumour progression were evaluated. The catalase-producing L. lactis strain used in this study was able to slightly increase catalase activities in DMH-treated mice (1.19 ± 0.08 U ml-1) and reduce H2O2 levels (3.4 ± 1.1 μM) compared to (i) animals that received the non-catalase-producing strain (1.00 ± 0.09 U ml-1, 9.0 ± 0.8 μM), and (ii) those that did not receive bacterial supplementation (1.06 ± 0.07 U ml-1, 10.0 ± 1.1 μM). Using the histopathological grading scale of chemically induced colorectal cancer, animals that received the catalase-producing L. lactis had a significantly lesser extent of colonic damage and inflammation (2.0 ± 0.4) compared to animals that received the non-catalase-producing L. lactis (4.0 ± 0.3) or those that did not receive bacterial supplementation (4.7 ± 0.5). The catalase-producing L. lactis strain used in this study was able to prevent tumour appearance in an experimental DMH-induced colon cancer model.
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Miyoshi, Anderson. Universidade Federal de Minas Gerais; Brasil
Fil: Langella, Philippe. Institut National de la Recherche Agronomique; Francia
Fil: Azevedo, Vaso. Universidade Federal de Minas Gerais; Brasil
Fil: Sesma, Fernando Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description Reactive oxygen species, such as hydrogen peroxide (H2O 2), are involved in various aspects of tumour development. Decreasing their levels can therefore be a promising approach for colon cancer prevention. The objective of this study was to evaluate the effect of catalase-producing Lactococcus lactis on the prevention of an experimental murine 1,2-dimethylhydrazine (DMH)-induced colon cancer. DMH-treated BALB/c mice received either a catalase-producing L. lactis strain or the isogenic non-catalase-producing strain as a control, whereas other untreated mice did not receive bacterial supplementation. Catalase activity and H2O 2 levels in intestinal fluids and blood samples were measured, and changes in the histology of the large intestines during tumour progression were evaluated. The catalase-producing L. lactis strain used in this study was able to slightly increase catalase activities in DMH-treated mice (1.19 ± 0.08 U ml-1) and reduce H2O2 levels (3.4 ± 1.1 μM) compared to (i) animals that received the non-catalase-producing strain (1.00 ± 0.09 U ml-1, 9.0 ± 0.8 μM), and (ii) those that did not receive bacterial supplementation (1.06 ± 0.07 U ml-1, 10.0 ± 1.1 μM). Using the histopathological grading scale of chemically induced colorectal cancer, animals that received the catalase-producing L. lactis had a significantly lesser extent of colonic damage and inflammation (2.0 ± 0.4) compared to animals that received the non-catalase-producing L. lactis (4.0 ± 0.3) or those that did not receive bacterial supplementation (4.7 ± 0.5). The catalase-producing L. lactis strain used in this study was able to prevent tumour appearance in an experimental DMH-induced colon cancer model.
publishDate 2008
dc.date.none.fl_str_mv 2008-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/56483
de Moreno, Maria Alejandra; Leblanc, Jean Guy Joseph; Perdigon, Gabriela del Valle; Miyoshi, Anderson; Langella, Philippe; et al.; Oral administration of a catalase-producing Lactococcus lactis can prevent a chemically induced colon cancer in mice; Society for General Microbiology; Journal Of Medical Microbiology; 57; 1; 1-2008; 100-105
0022-2615
1473-5644
CONICET Digital
CONICET
url http://hdl.handle.net/11336/56483
identifier_str_mv de Moreno, Maria Alejandra; Leblanc, Jean Guy Joseph; Perdigon, Gabriela del Valle; Miyoshi, Anderson; Langella, Philippe; et al.; Oral administration of a catalase-producing Lactococcus lactis can prevent a chemically induced colon cancer in mice; Society for General Microbiology; Journal Of Medical Microbiology; 57; 1; 1-2008; 100-105
0022-2615
1473-5644
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1099/jmm.0.47403-0
info:eu-repo/semantics/altIdentifier/url/http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.47403-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for General Microbiology
publisher.none.fl_str_mv Society for General Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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