Co-cultivation of haematococcus pluvialis and chlorella sp. as a novel strategy for microalgal-bases biotechnology

Autores
Scodelaro Bilbao, Paola Gabriela; Almeyda, María Delfina; Bolletta, Micaela; Leonardi, Patricia Ines
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Microalgae have been identified as potential sources of valuable products with many commercial applications including food supplements, feed additives and biofuel feedstocks. They are innovative production platforms since, in order to adapt to growth conditions, they synthesize various metabolites. However, the synthesis of these biomolecules requires an adequate selection of microalgal species, a deep knowledge of their biology and physiology, as well as rigorous evaluation of cultivation strategies. Monocultures have been the preferred production route in the bio-industry. Nevertheless, from a biotechnological perspective, it is necessary to develop successful cultivation technologies to increase their productivity, in terms of biomass and availability of biomolecules. In this way, there is increasing interest in the use of co-cultures to deal with contamination issues, and simultaneously increase productivity and product diversity. Thus, in this work our purpose was to analyze co-cultivations of two different microalgal strains in terms of biomass production and product availability. For this end, Haematococcus pluvialis and Chlorella sp., two important carotenoid producers, were selected for co-cultivations in an appropriate culture medium at 22oC for 10 days. Then, cell number, dry weight, chlorophyll and carotenoid quantification and autofluorescence, Red Nile (RN) fluorescence, and triacylglyceride (TAG) and sterol contents were analyzed. The results revealed that co-cultivation based on 50% H. pluvialis and 50% Chlorella sp. prevented population domination of one strain over the other. In addition, this co-cultivation condition showed the highest values in terms of cell density and dry weight. Flow cytometry analyses also shown the maximum RN fluorescence and carotenoid autofluorescence within this experimental condition. In addition, in co-cultures based on 50% H. pluvialis and 50% Chlorella sp., carotenoid autofluorescence was accompanied by the greatest increase in the antioxidant capacity and in the amount of total carotenoids. Moreover, thin layer chromatography coupled to spectrophotometric quantification also showed highest TAG and sterol contents. The results suggest that the co-cultivation system based on 50% H. pluvialis and 50% Chlorella sp. may be a successful strategy to enhance biomass yield and the obtention of value-added products, supporting the development of a microalgal-based biotechnological process.
Fil: Scodelaro Bilbao, Paola Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Almeyda, María Delfina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Bolletta, Micaela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LVII SAIB Meeting; XVI SAMIGE Meeting
Online
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
Clorophyta
Coculture
Biomass
Lipid
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/177175
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Co-cultivation of haematococcus pluvialis and chlorella sp. as a novel strategy for microalgal-bases biotechnologyScodelaro Bilbao, Paola GabrielaAlmeyda, María DelfinaBolletta, MicaelaLeonardi, Patricia InesClorophytaCocultureBiomassLipidhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Microalgae have been identified as potential sources of valuable products with many commercial applications including food supplements, feed additives and biofuel feedstocks. They are innovative production platforms since, in order to adapt to growth conditions, they synthesize various metabolites. However, the synthesis of these biomolecules requires an adequate selection of microalgal species, a deep knowledge of their biology and physiology, as well as rigorous evaluation of cultivation strategies. Monocultures have been the preferred production route in the bio-industry. Nevertheless, from a biotechnological perspective, it is necessary to develop successful cultivation technologies to increase their productivity, in terms of biomass and availability of biomolecules. In this way, there is increasing interest in the use of co-cultures to deal with contamination issues, and simultaneously increase productivity and product diversity. Thus, in this work our purpose was to analyze co-cultivations of two different microalgal strains in terms of biomass production and product availability. For this end, Haematococcus pluvialis and Chlorella sp., two important carotenoid producers, were selected for co-cultivations in an appropriate culture medium at 22oC for 10 days. Then, cell number, dry weight, chlorophyll and carotenoid quantification and autofluorescence, Red Nile (RN) fluorescence, and triacylglyceride (TAG) and sterol contents were analyzed. The results revealed that co-cultivation based on 50% H. pluvialis and 50% Chlorella sp. prevented population domination of one strain over the other. In addition, this co-cultivation condition showed the highest values in terms of cell density and dry weight. Flow cytometry analyses also shown the maximum RN fluorescence and carotenoid autofluorescence within this experimental condition. In addition, in co-cultures based on 50% H. pluvialis and 50% Chlorella sp., carotenoid autofluorescence was accompanied by the greatest increase in the antioxidant capacity and in the amount of total carotenoids. Moreover, thin layer chromatography coupled to spectrophotometric quantification also showed highest TAG and sterol contents. The results suggest that the co-cultivation system based on 50% H. pluvialis and 50% Chlorella sp. may be a successful strategy to enhance biomass yield and the obtention of value-added products, supporting the development of a microalgal-based biotechnological process.Fil: Scodelaro Bilbao, Paola Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Almeyda, María Delfina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Bolletta, Micaela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaLVII SAIB Meeting; XVI SAMIGE MeetingOnlineArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularSociedad Argentina de Investigación Bioquímica y Biología Molecular2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/177175Co-cultivation of haematococcus pluvialis and chlorella sp. as a novel strategy for microalgal-bases biotechnology; LVII SAIB Meeting; XVI SAMIGE Meeting; Online; Argentina; 2021; 1-10327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://congresos.g2consultora.com/wp-content/uploads/2021/10/Biocell-Preprint-SAIB-SAMIGE-2021.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:18Zoai:ri.conicet.gov.ar:11336/177175instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:19.046CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Co-cultivation of haematococcus pluvialis and chlorella sp. as a novel strategy for microalgal-bases biotechnology
title Co-cultivation of haematococcus pluvialis and chlorella sp. as a novel strategy for microalgal-bases biotechnology
spellingShingle Co-cultivation of haematococcus pluvialis and chlorella sp. as a novel strategy for microalgal-bases biotechnology
Scodelaro Bilbao, Paola Gabriela
Clorophyta
Coculture
Biomass
Lipid
title_short Co-cultivation of haematococcus pluvialis and chlorella sp. as a novel strategy for microalgal-bases biotechnology
title_full Co-cultivation of haematococcus pluvialis and chlorella sp. as a novel strategy for microalgal-bases biotechnology
title_fullStr Co-cultivation of haematococcus pluvialis and chlorella sp. as a novel strategy for microalgal-bases biotechnology
title_full_unstemmed Co-cultivation of haematococcus pluvialis and chlorella sp. as a novel strategy for microalgal-bases biotechnology
title_sort Co-cultivation of haematococcus pluvialis and chlorella sp. as a novel strategy for microalgal-bases biotechnology
dc.creator.none.fl_str_mv Scodelaro Bilbao, Paola Gabriela
Almeyda, María Delfina
Bolletta, Micaela
Leonardi, Patricia Ines
author Scodelaro Bilbao, Paola Gabriela
author_facet Scodelaro Bilbao, Paola Gabriela
Almeyda, María Delfina
Bolletta, Micaela
Leonardi, Patricia Ines
author_role author
author2 Almeyda, María Delfina
Bolletta, Micaela
Leonardi, Patricia Ines
author2_role author
author
author
dc.subject.none.fl_str_mv Clorophyta
Coculture
Biomass
Lipid
topic Clorophyta
Coculture
Biomass
Lipid
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Microalgae have been identified as potential sources of valuable products with many commercial applications including food supplements, feed additives and biofuel feedstocks. They are innovative production platforms since, in order to adapt to growth conditions, they synthesize various metabolites. However, the synthesis of these biomolecules requires an adequate selection of microalgal species, a deep knowledge of their biology and physiology, as well as rigorous evaluation of cultivation strategies. Monocultures have been the preferred production route in the bio-industry. Nevertheless, from a biotechnological perspective, it is necessary to develop successful cultivation technologies to increase their productivity, in terms of biomass and availability of biomolecules. In this way, there is increasing interest in the use of co-cultures to deal with contamination issues, and simultaneously increase productivity and product diversity. Thus, in this work our purpose was to analyze co-cultivations of two different microalgal strains in terms of biomass production and product availability. For this end, Haematococcus pluvialis and Chlorella sp., two important carotenoid producers, were selected for co-cultivations in an appropriate culture medium at 22oC for 10 days. Then, cell number, dry weight, chlorophyll and carotenoid quantification and autofluorescence, Red Nile (RN) fluorescence, and triacylglyceride (TAG) and sterol contents were analyzed. The results revealed that co-cultivation based on 50% H. pluvialis and 50% Chlorella sp. prevented population domination of one strain over the other. In addition, this co-cultivation condition showed the highest values in terms of cell density and dry weight. Flow cytometry analyses also shown the maximum RN fluorescence and carotenoid autofluorescence within this experimental condition. In addition, in co-cultures based on 50% H. pluvialis and 50% Chlorella sp., carotenoid autofluorescence was accompanied by the greatest increase in the antioxidant capacity and in the amount of total carotenoids. Moreover, thin layer chromatography coupled to spectrophotometric quantification also showed highest TAG and sterol contents. The results suggest that the co-cultivation system based on 50% H. pluvialis and 50% Chlorella sp. may be a successful strategy to enhance biomass yield and the obtention of value-added products, supporting the development of a microalgal-based biotechnological process.
Fil: Scodelaro Bilbao, Paola Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Almeyda, María Delfina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Bolletta, Micaela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LVII SAIB Meeting; XVI SAMIGE Meeting
Online
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description Microalgae have been identified as potential sources of valuable products with many commercial applications including food supplements, feed additives and biofuel feedstocks. They are innovative production platforms since, in order to adapt to growth conditions, they synthesize various metabolites. However, the synthesis of these biomolecules requires an adequate selection of microalgal species, a deep knowledge of their biology and physiology, as well as rigorous evaluation of cultivation strategies. Monocultures have been the preferred production route in the bio-industry. Nevertheless, from a biotechnological perspective, it is necessary to develop successful cultivation technologies to increase their productivity, in terms of biomass and availability of biomolecules. In this way, there is increasing interest in the use of co-cultures to deal with contamination issues, and simultaneously increase productivity and product diversity. Thus, in this work our purpose was to analyze co-cultivations of two different microalgal strains in terms of biomass production and product availability. For this end, Haematococcus pluvialis and Chlorella sp., two important carotenoid producers, were selected for co-cultivations in an appropriate culture medium at 22oC for 10 days. Then, cell number, dry weight, chlorophyll and carotenoid quantification and autofluorescence, Red Nile (RN) fluorescence, and triacylglyceride (TAG) and sterol contents were analyzed. The results revealed that co-cultivation based on 50% H. pluvialis and 50% Chlorella sp. prevented population domination of one strain over the other. In addition, this co-cultivation condition showed the highest values in terms of cell density and dry weight. Flow cytometry analyses also shown the maximum RN fluorescence and carotenoid autofluorescence within this experimental condition. In addition, in co-cultures based on 50% H. pluvialis and 50% Chlorella sp., carotenoid autofluorescence was accompanied by the greatest increase in the antioxidant capacity and in the amount of total carotenoids. Moreover, thin layer chromatography coupled to spectrophotometric quantification also showed highest TAG and sterol contents. The results suggest that the co-cultivation system based on 50% H. pluvialis and 50% Chlorella sp. may be a successful strategy to enhance biomass yield and the obtention of value-added products, supporting the development of a microalgal-based biotechnological process.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/177175
Co-cultivation of haematococcus pluvialis and chlorella sp. as a novel strategy for microalgal-bases biotechnology; LVII SAIB Meeting; XVI SAMIGE Meeting; Online; Argentina; 2021; 1-1
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/177175
identifier_str_mv Co-cultivation of haematococcus pluvialis and chlorella sp. as a novel strategy for microalgal-bases biotechnology; LVII SAIB Meeting; XVI SAMIGE Meeting; Online; Argentina; 2021; 1-1
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://congresos.g2consultora.com/wp-content/uploads/2021/10/Biocell-Preprint-SAIB-SAMIGE-2021.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación Bioquímica y Biología Molecular
publisher.none.fl_str_mv Sociedad Argentina de Investigación Bioquímica y Biología Molecular
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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