New insights into lipid metabolism of Haematococcus pluvialis in response to high-light stress
- Autores
- Scodelaro Bilbao, Paola Gabriela; Salvador, Gabriela Alejandra; Leonardi, Patricia Ines
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- The oleaginous unicellular microalga Haematococcus pluvialis is being intensely studied as a source of carotenoids and lipids with potential biotechnological and biomedical applications. The aim of this study was to analyze the effect of high-light stress on the regulation of triacylglyceride (TAG) and sterol synthesis in H. pluvialis UTEX 2505. After 24 hours of high-light exposure, both lipid classes significantly increased compared to the control condition. As expected, the use of inhibitors of the Kennedy pathway cerulenin, propranolol, xanthohumol and A922500 significantly decreased TAG synthesis under high-light stress, while mevastatin and 25-hydroxycholesterol reduced sterol content. These results were coincident with qPCR and Western blot analyses and also revealed some of the enzymes of H. pluvialis involved in lipid synthesis in response to high-light. In addition, we observed that Kennedy pathway inhibitors yet reduced sterol content while sterol biosynthesis inhibitors also diminished TAG production, denoting a coordination between both synthesis pathways. Fosmidomycin, a specific inhibitor of the non-mevalonate pathway of isoprenoid biosynthesis, reduced total carotenoid content and antioxidant capacity together with TAG and sterol content. Finally, inhibition of carotenoid, TAG and sterol synthesis significantly reduced cell viability suggesting their participation in H. pluvialis survival and adaption to high irradiance. These findings reveal features of lipid synthesis and function of H. pluvialis under high-light stress, and points towards new possible molecular targets to enhance lipid production in microalgae.
Fil: Scodelaro Bilbao, Paola Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
54th Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology
Paraná
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular - Materia
-
MICROALGAE
TRIACYLGLYCERIDES
STEROLS
GREEN ALGAE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/224036
Ver los metadatos del registro completo
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New insights into lipid metabolism of Haematococcus pluvialis in response to high-light stressScodelaro Bilbao, Paola GabrielaSalvador, Gabriela AlejandraLeonardi, Patricia InesMICROALGAETRIACYLGLYCERIDESSTEROLSGREEN ALGAEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The oleaginous unicellular microalga Haematococcus pluvialis is being intensely studied as a source of carotenoids and lipids with potential biotechnological and biomedical applications. The aim of this study was to analyze the effect of high-light stress on the regulation of triacylglyceride (TAG) and sterol synthesis in H. pluvialis UTEX 2505. After 24 hours of high-light exposure, both lipid classes significantly increased compared to the control condition. As expected, the use of inhibitors of the Kennedy pathway cerulenin, propranolol, xanthohumol and A922500 significantly decreased TAG synthesis under high-light stress, while mevastatin and 25-hydroxycholesterol reduced sterol content. These results were coincident with qPCR and Western blot analyses and also revealed some of the enzymes of H. pluvialis involved in lipid synthesis in response to high-light. In addition, we observed that Kennedy pathway inhibitors yet reduced sterol content while sterol biosynthesis inhibitors also diminished TAG production, denoting a coordination between both synthesis pathways. Fosmidomycin, a specific inhibitor of the non-mevalonate pathway of isoprenoid biosynthesis, reduced total carotenoid content and antioxidant capacity together with TAG and sterol content. Finally, inhibition of carotenoid, TAG and sterol synthesis significantly reduced cell viability suggesting their participation in H. pluvialis survival and adaption to high irradiance. These findings reveal features of lipid synthesis and function of H. pluvialis under high-light stress, and points towards new possible molecular targets to enhance lipid production in microalgae.Fil: Scodelaro Bilbao, Paola Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina54th Annual Meeting of the Argentine Society for Biochemistry and Molecular BiologyParanáArgentinaSociedad Argentina de Investigación Bioquímica y Biología MolecularTech Science Press2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/224036New insights into lipid metabolism of Haematococcus pluvialis in response to high-light stress; 54th Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology; Paraná; Argentina; 2018; 90-901667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2018.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:37Zoai:ri.conicet.gov.ar:11336/224036instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:37.393CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
New insights into lipid metabolism of Haematococcus pluvialis in response to high-light stress |
| title |
New insights into lipid metabolism of Haematococcus pluvialis in response to high-light stress |
| spellingShingle |
New insights into lipid metabolism of Haematococcus pluvialis in response to high-light stress Scodelaro Bilbao, Paola Gabriela MICROALGAE TRIACYLGLYCERIDES STEROLS GREEN ALGAE |
| title_short |
New insights into lipid metabolism of Haematococcus pluvialis in response to high-light stress |
| title_full |
New insights into lipid metabolism of Haematococcus pluvialis in response to high-light stress |
| title_fullStr |
New insights into lipid metabolism of Haematococcus pluvialis in response to high-light stress |
| title_full_unstemmed |
New insights into lipid metabolism of Haematococcus pluvialis in response to high-light stress |
| title_sort |
New insights into lipid metabolism of Haematococcus pluvialis in response to high-light stress |
| dc.creator.none.fl_str_mv |
Scodelaro Bilbao, Paola Gabriela Salvador, Gabriela Alejandra Leonardi, Patricia Ines |
| author |
Scodelaro Bilbao, Paola Gabriela |
| author_facet |
Scodelaro Bilbao, Paola Gabriela Salvador, Gabriela Alejandra Leonardi, Patricia Ines |
| author_role |
author |
| author2 |
Salvador, Gabriela Alejandra Leonardi, Patricia Ines |
| author2_role |
author author |
| dc.subject.none.fl_str_mv |
MICROALGAE TRIACYLGLYCERIDES STEROLS GREEN ALGAE |
| topic |
MICROALGAE TRIACYLGLYCERIDES STEROLS GREEN ALGAE |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
The oleaginous unicellular microalga Haematococcus pluvialis is being intensely studied as a source of carotenoids and lipids with potential biotechnological and biomedical applications. The aim of this study was to analyze the effect of high-light stress on the regulation of triacylglyceride (TAG) and sterol synthesis in H. pluvialis UTEX 2505. After 24 hours of high-light exposure, both lipid classes significantly increased compared to the control condition. As expected, the use of inhibitors of the Kennedy pathway cerulenin, propranolol, xanthohumol and A922500 significantly decreased TAG synthesis under high-light stress, while mevastatin and 25-hydroxycholesterol reduced sterol content. These results were coincident with qPCR and Western blot analyses and also revealed some of the enzymes of H. pluvialis involved in lipid synthesis in response to high-light. In addition, we observed that Kennedy pathway inhibitors yet reduced sterol content while sterol biosynthesis inhibitors also diminished TAG production, denoting a coordination between both synthesis pathways. Fosmidomycin, a specific inhibitor of the non-mevalonate pathway of isoprenoid biosynthesis, reduced total carotenoid content and antioxidant capacity together with TAG and sterol content. Finally, inhibition of carotenoid, TAG and sterol synthesis significantly reduced cell viability suggesting their participation in H. pluvialis survival and adaption to high irradiance. These findings reveal features of lipid synthesis and function of H. pluvialis under high-light stress, and points towards new possible molecular targets to enhance lipid production in microalgae. Fil: Scodelaro Bilbao, Paola Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina Fil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina 54th Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Paraná Argentina Sociedad Argentina de Investigación Bioquímica y Biología Molecular |
| description |
The oleaginous unicellular microalga Haematococcus pluvialis is being intensely studied as a source of carotenoids and lipids with potential biotechnological and biomedical applications. The aim of this study was to analyze the effect of high-light stress on the regulation of triacylglyceride (TAG) and sterol synthesis in H. pluvialis UTEX 2505. After 24 hours of high-light exposure, both lipid classes significantly increased compared to the control condition. As expected, the use of inhibitors of the Kennedy pathway cerulenin, propranolol, xanthohumol and A922500 significantly decreased TAG synthesis under high-light stress, while mevastatin and 25-hydroxycholesterol reduced sterol content. These results were coincident with qPCR and Western blot analyses and also revealed some of the enzymes of H. pluvialis involved in lipid synthesis in response to high-light. In addition, we observed that Kennedy pathway inhibitors yet reduced sterol content while sterol biosynthesis inhibitors also diminished TAG production, denoting a coordination between both synthesis pathways. Fosmidomycin, a specific inhibitor of the non-mevalonate pathway of isoprenoid biosynthesis, reduced total carotenoid content and antioxidant capacity together with TAG and sterol content. Finally, inhibition of carotenoid, TAG and sterol synthesis significantly reduced cell viability suggesting their participation in H. pluvialis survival and adaption to high irradiance. These findings reveal features of lipid synthesis and function of H. pluvialis under high-light stress, and points towards new possible molecular targets to enhance lipid production in microalgae. |
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2018 |
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2018 |
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http://hdl.handle.net/11336/224036 New insights into lipid metabolism of Haematococcus pluvialis in response to high-light stress; 54th Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology; Paraná; Argentina; 2018; 90-90 1667-5746 CONICET Digital CONICET |
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New insights into lipid metabolism of Haematococcus pluvialis in response to high-light stress; 54th Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology; Paraná; Argentina; 2018; 90-90 1667-5746 CONICET Digital CONICET |
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eng |
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