The adhesion plaque mediates fluid discharge and duplication of the contractile vacuole complex in Trypanosoma cruzi

Autores
Augusto, Ingrid; Girard Dias, Wendell; Schoijet, Alejandra Cecilia; Alonso, Guillermo Daniel; Jimenez, Veronica; de Souza, Wanderley; Miranda, Kildare
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work explores the intricate process of osmoregulation in Trypanosoma cruzi, the causative agent of Chagas disease, with a specific focus on the mechanisms of fluid discharge by the contractile vacuole complex (CVC) and the role of the adhesion plaque (AP), a structure whose densities are located in the membrane domain shared by the CVC and the flagellar pocket. Cryopreparation of T. cruzi samples, combined with volume electron microscopy techniques, allowed for a comprehensive analysis of the essential mechanisms underlying the structural changes that take place in the AP during osmotic stress. Remodeling of the AP coupled to membrane fusion events leads to the formation of pores that connect the flagellar pocket and the CVC. The fluid discharge process followed sequential steps of pore opening, expansion and closure, to allow membrane fusion. Additionally, this study uncovers structural variations in the CVC during cellular replication, providing insights into the cellular biology and physiology of trypanosomatids.
Fil: Augusto, Ingrid. Universidade Federal do Rio de Janeiro; Brasil
Fil: Girard Dias, Wendell. Universidade Federal do Rio de Janeiro; Brasil. Ministerio de Salud de Brasil. Fundación Oswaldo Cruz. Instituto Oswaldo Cruz;
Fil: Schoijet, Alejandra Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Alonso, Guillermo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Jimenez, Veronica. California State University. College Of Natural Science And Mathematics; Estados Unidos
Fil: de Souza, Wanderley. Universidade Federal do Rio de Janeiro; Brasil
Fil: Miranda, Kildare. Universidade Federal do Rio de Janeiro; Brasil
Materia
ADHESION PLAQUE
CONTRACTILE VACUOLE
OSMOREGULATION
CHAGAS DISEASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/280841
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The adhesion plaque mediates fluid discharge and duplication of the contractile vacuole complex in Trypanosoma cruziAugusto, IngridGirard Dias, WendellSchoijet, Alejandra CeciliaAlonso, Guillermo DanielJimenez, Veronicade Souza, WanderleyMiranda, KildareADHESION PLAQUECONTRACTILE VACUOLEOSMOREGULATIONCHAGAS DISEASEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1This work explores the intricate process of osmoregulation in Trypanosoma cruzi, the causative agent of Chagas disease, with a specific focus on the mechanisms of fluid discharge by the contractile vacuole complex (CVC) and the role of the adhesion plaque (AP), a structure whose densities are located in the membrane domain shared by the CVC and the flagellar pocket. Cryopreparation of T. cruzi samples, combined with volume electron microscopy techniques, allowed for a comprehensive analysis of the essential mechanisms underlying the structural changes that take place in the AP during osmotic stress. Remodeling of the AP coupled to membrane fusion events leads to the formation of pores that connect the flagellar pocket and the CVC. The fluid discharge process followed sequential steps of pore opening, expansion and closure, to allow membrane fusion. Additionally, this study uncovers structural variations in the CVC during cellular replication, providing insights into the cellular biology and physiology of trypanosomatids.Fil: Augusto, Ingrid. Universidade Federal do Rio de Janeiro; BrasilFil: Girard Dias, Wendell. Universidade Federal do Rio de Janeiro; Brasil. Ministerio de Salud de Brasil. Fundación Oswaldo Cruz. Instituto Oswaldo Cruz;Fil: Schoijet, Alejandra Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Alonso, Guillermo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Jimenez, Veronica. California State University. College Of Natural Science And Mathematics; Estados UnidosFil: de Souza, Wanderley. Universidade Federal do Rio de Janeiro; BrasilFil: Miranda, Kildare. Universidade Federal do Rio de Janeiro; BrasilCompany of Biologists2025-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/280841Augusto, Ingrid; Girard Dias, Wendell; Schoijet, Alejandra Cecilia; Alonso, Guillermo Daniel; Jimenez, Veronica; et al.; The adhesion plaque mediates fluid discharge and duplication of the contractile vacuole complex in Trypanosoma cruzi; Company of Biologists; Journal of Cell Science; 138; 15; 8-2025; 1-120021-9533CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.biologists.com/jcs/article/138/15/jcs263810/368816/The-adhesion-plaque-mediates-fluid-discharge-andinfo:eu-repo/semantics/altIdentifier/doi/10.1242/jcs.263810info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T09:57:37Zoai:ri.conicet.gov.ar:11336/280841instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 09:57:37.697CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The adhesion plaque mediates fluid discharge and duplication of the contractile vacuole complex in Trypanosoma cruzi
title The adhesion plaque mediates fluid discharge and duplication of the contractile vacuole complex in Trypanosoma cruzi
spellingShingle The adhesion plaque mediates fluid discharge and duplication of the contractile vacuole complex in Trypanosoma cruzi
Augusto, Ingrid
ADHESION PLAQUE
CONTRACTILE VACUOLE
OSMOREGULATION
CHAGAS DISEASE
title_short The adhesion plaque mediates fluid discharge and duplication of the contractile vacuole complex in Trypanosoma cruzi
title_full The adhesion plaque mediates fluid discharge and duplication of the contractile vacuole complex in Trypanosoma cruzi
title_fullStr The adhesion plaque mediates fluid discharge and duplication of the contractile vacuole complex in Trypanosoma cruzi
title_full_unstemmed The adhesion plaque mediates fluid discharge and duplication of the contractile vacuole complex in Trypanosoma cruzi
title_sort The adhesion plaque mediates fluid discharge and duplication of the contractile vacuole complex in Trypanosoma cruzi
dc.creator.none.fl_str_mv Augusto, Ingrid
Girard Dias, Wendell
Schoijet, Alejandra Cecilia
Alonso, Guillermo Daniel
Jimenez, Veronica
de Souza, Wanderley
Miranda, Kildare
author Augusto, Ingrid
author_facet Augusto, Ingrid
Girard Dias, Wendell
Schoijet, Alejandra Cecilia
Alonso, Guillermo Daniel
Jimenez, Veronica
de Souza, Wanderley
Miranda, Kildare
author_role author
author2 Girard Dias, Wendell
Schoijet, Alejandra Cecilia
Alonso, Guillermo Daniel
Jimenez, Veronica
de Souza, Wanderley
Miranda, Kildare
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ADHESION PLAQUE
CONTRACTILE VACUOLE
OSMOREGULATION
CHAGAS DISEASE
topic ADHESION PLAQUE
CONTRACTILE VACUOLE
OSMOREGULATION
CHAGAS DISEASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This work explores the intricate process of osmoregulation in Trypanosoma cruzi, the causative agent of Chagas disease, with a specific focus on the mechanisms of fluid discharge by the contractile vacuole complex (CVC) and the role of the adhesion plaque (AP), a structure whose densities are located in the membrane domain shared by the CVC and the flagellar pocket. Cryopreparation of T. cruzi samples, combined with volume electron microscopy techniques, allowed for a comprehensive analysis of the essential mechanisms underlying the structural changes that take place in the AP during osmotic stress. Remodeling of the AP coupled to membrane fusion events leads to the formation of pores that connect the flagellar pocket and the CVC. The fluid discharge process followed sequential steps of pore opening, expansion and closure, to allow membrane fusion. Additionally, this study uncovers structural variations in the CVC during cellular replication, providing insights into the cellular biology and physiology of trypanosomatids.
Fil: Augusto, Ingrid. Universidade Federal do Rio de Janeiro; Brasil
Fil: Girard Dias, Wendell. Universidade Federal do Rio de Janeiro; Brasil. Ministerio de Salud de Brasil. Fundación Oswaldo Cruz. Instituto Oswaldo Cruz;
Fil: Schoijet, Alejandra Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Alonso, Guillermo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Jimenez, Veronica. California State University. College Of Natural Science And Mathematics; Estados Unidos
Fil: de Souza, Wanderley. Universidade Federal do Rio de Janeiro; Brasil
Fil: Miranda, Kildare. Universidade Federal do Rio de Janeiro; Brasil
description This work explores the intricate process of osmoregulation in Trypanosoma cruzi, the causative agent of Chagas disease, with a specific focus on the mechanisms of fluid discharge by the contractile vacuole complex (CVC) and the role of the adhesion plaque (AP), a structure whose densities are located in the membrane domain shared by the CVC and the flagellar pocket. Cryopreparation of T. cruzi samples, combined with volume electron microscopy techniques, allowed for a comprehensive analysis of the essential mechanisms underlying the structural changes that take place in the AP during osmotic stress. Remodeling of the AP coupled to membrane fusion events leads to the formation of pores that connect the flagellar pocket and the CVC. The fluid discharge process followed sequential steps of pore opening, expansion and closure, to allow membrane fusion. Additionally, this study uncovers structural variations in the CVC during cellular replication, providing insights into the cellular biology and physiology of trypanosomatids.
publishDate 2025
dc.date.none.fl_str_mv 2025-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/280841
Augusto, Ingrid; Girard Dias, Wendell; Schoijet, Alejandra Cecilia; Alonso, Guillermo Daniel; Jimenez, Veronica; et al.; The adhesion plaque mediates fluid discharge and duplication of the contractile vacuole complex in Trypanosoma cruzi; Company of Biologists; Journal of Cell Science; 138; 15; 8-2025; 1-12
0021-9533
CONICET Digital
CONICET
url http://hdl.handle.net/11336/280841
identifier_str_mv Augusto, Ingrid; Girard Dias, Wendell; Schoijet, Alejandra Cecilia; Alonso, Guillermo Daniel; Jimenez, Veronica; et al.; The adhesion plaque mediates fluid discharge and duplication of the contractile vacuole complex in Trypanosoma cruzi; Company of Biologists; Journal of Cell Science; 138; 15; 8-2025; 1-12
0021-9533
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.biologists.com/jcs/article/138/15/jcs263810/368816/The-adhesion-plaque-mediates-fluid-discharge-and
info:eu-repo/semantics/altIdentifier/doi/10.1242/jcs.263810
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1858304748200919040
score 13.176822

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