Trypanosoma cruzi invasion in non-phagocytic cells: an ultrastructural study

Autores
Cueto, Juan Agustin; Santos Barrias, Emile; de Souza, Wanderley; Romano, Patricia Silvia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trypanosoma cruzi is the causative agent of Chagas disease. This parasite requires the intracellular niche in order to proliferate and disseminate the infection. After invasion, T. cruzi resides temporarily in an acidic vacuole which is lysed by a not well-understood mechanism. Transmission electron microscopy was used to describe the process of T. cruzi escape from the parasitophorous vacuole over the time. Using HeLa (non-professional phagocytic cells) as host cell, we observed that recently internalized parasites reside in a membrane-bounded vacuole. A few hours later, the first sign of vacuole disruption appeared as membrane discontinuities. This observation was followed by a progressive vacuole swelling as evidenced by an electron-lucent halo between the parasite and the vacuole membrane. Apparently, the vacuole membrane remnants reorganized as small vesicles that eventually disappeared from the vicinity of the parasites. Finally, parasites reach the host cell cytosol where replication takes place. The thorough ultrastructural description of this process set the base for a comprehensive understanding of the parasite-host cell interaction and, thus open the possibility of new therapeutic intervention strategies.
Fil: Cueto, Juan Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Santos Barrias, Emile. Instituto Nacional de Metrologia, Qualidade e Tecnologia-Inmetro, Rio de Janeiro; Brasil
Fil: de Souza, Wanderley. Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Centro de Ciência da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro; Brasil. Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagens e Centro Nacional de Bioimagens-CENABIO, Universidade Federal do Rio de Janeiro, Rio de Janeiro; Brasil
Fil: Romano, Patricia Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Materia
PARASITOPHOROUS VACUOLE
PATHOGEN
PARASITES
CHAGAS DISEASE
INFECTIOUS DISEASES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95263
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/95263
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Trypanosoma cruzi invasion in non-phagocytic cells: an ultrastructural studyCueto, Juan AgustinSantos Barrias, Emilede Souza, WanderleyRomano, Patricia SilviaPARASITOPHOROUS VACUOLEPATHOGENPARASITESCHAGAS DISEASEINFECTIOUS DISEASEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Trypanosoma cruzi is the causative agent of Chagas disease. This parasite requires the intracellular niche in order to proliferate and disseminate the infection. After invasion, T. cruzi resides temporarily in an acidic vacuole which is lysed by a not well-understood mechanism. Transmission electron microscopy was used to describe the process of T. cruzi escape from the parasitophorous vacuole over the time. Using HeLa (non-professional phagocytic cells) as host cell, we observed that recently internalized parasites reside in a membrane-bounded vacuole. A few hours later, the first sign of vacuole disruption appeared as membrane discontinuities. This observation was followed by a progressive vacuole swelling as evidenced by an electron-lucent halo between the parasite and the vacuole membrane. Apparently, the vacuole membrane remnants reorganized as small vesicles that eventually disappeared from the vicinity of the parasites. Finally, parasites reach the host cell cytosol where replication takes place. The thorough ultrastructural description of this process set the base for a comprehensive understanding of the parasite-host cell interaction and, thus open the possibility of new therapeutic intervention strategies.Fil: Cueto, Juan Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Santos Barrias, Emile. Instituto Nacional de Metrologia, Qualidade e Tecnologia-Inmetro, Rio de Janeiro; BrasilFil: de Souza, Wanderley. Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Centro de Ciência da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro; Brasil. Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagens e Centro Nacional de Bioimagens-CENABIO, Universidade Federal do Rio de Janeiro, Rio de Janeiro; BrasilFil: Romano, Patricia Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaInstituto de Histología y Embriología "Dr. Mario Burgos"2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95263Cueto, Juan Agustin; Santos Barrias, Emile; de Souza, Wanderley; Romano, Patricia Silvia; Trypanosoma cruzi invasion in non-phagocytic cells: an ultrastructural study; Instituto de Histología y Embriología "Dr. Mario Burgos"; Biocell; 42; 3; 12-2018; 105-1080327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://biocell.institute/index.php/BioCell/article/view/105-108info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:40Zoai:ri.conicet.gov.ar:11336/95263instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:40.568CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Trypanosoma cruzi invasion in non-phagocytic cells: an ultrastructural study
title Trypanosoma cruzi invasion in non-phagocytic cells: an ultrastructural study
spellingShingle Trypanosoma cruzi invasion in non-phagocytic cells: an ultrastructural study
Cueto, Juan Agustin
PARASITOPHOROUS VACUOLE
PATHOGEN
PARASITES
CHAGAS DISEASE
INFECTIOUS DISEASES
title_short Trypanosoma cruzi invasion in non-phagocytic cells: an ultrastructural study
title_full Trypanosoma cruzi invasion in non-phagocytic cells: an ultrastructural study
title_fullStr Trypanosoma cruzi invasion in non-phagocytic cells: an ultrastructural study
title_full_unstemmed Trypanosoma cruzi invasion in non-phagocytic cells: an ultrastructural study
title_sort Trypanosoma cruzi invasion in non-phagocytic cells: an ultrastructural study
dc.creator.none.fl_str_mv Cueto, Juan Agustin
Santos Barrias, Emile
de Souza, Wanderley
Romano, Patricia Silvia
author Cueto, Juan Agustin
author_facet Cueto, Juan Agustin
Santos Barrias, Emile
de Souza, Wanderley
Romano, Patricia Silvia
author_role author
author2 Santos Barrias, Emile
de Souza, Wanderley
Romano, Patricia Silvia
author2_role author
author
author
dc.subject.none.fl_str_mv PARASITOPHOROUS VACUOLE
PATHOGEN
PARASITES
CHAGAS DISEASE
INFECTIOUS DISEASES
topic PARASITOPHOROUS VACUOLE
PATHOGEN
PARASITES
CHAGAS DISEASE
INFECTIOUS DISEASES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Trypanosoma cruzi is the causative agent of Chagas disease. This parasite requires the intracellular niche in order to proliferate and disseminate the infection. After invasion, T. cruzi resides temporarily in an acidic vacuole which is lysed by a not well-understood mechanism. Transmission electron microscopy was used to describe the process of T. cruzi escape from the parasitophorous vacuole over the time. Using HeLa (non-professional phagocytic cells) as host cell, we observed that recently internalized parasites reside in a membrane-bounded vacuole. A few hours later, the first sign of vacuole disruption appeared as membrane discontinuities. This observation was followed by a progressive vacuole swelling as evidenced by an electron-lucent halo between the parasite and the vacuole membrane. Apparently, the vacuole membrane remnants reorganized as small vesicles that eventually disappeared from the vicinity of the parasites. Finally, parasites reach the host cell cytosol where replication takes place. The thorough ultrastructural description of this process set the base for a comprehensive understanding of the parasite-host cell interaction and, thus open the possibility of new therapeutic intervention strategies.
Fil: Cueto, Juan Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Santos Barrias, Emile. Instituto Nacional de Metrologia, Qualidade e Tecnologia-Inmetro, Rio de Janeiro; Brasil
Fil: de Souza, Wanderley. Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Centro de Ciência da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro; Brasil. Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagens e Centro Nacional de Bioimagens-CENABIO, Universidade Federal do Rio de Janeiro, Rio de Janeiro; Brasil
Fil: Romano, Patricia Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
description Trypanosoma cruzi is the causative agent of Chagas disease. This parasite requires the intracellular niche in order to proliferate and disseminate the infection. After invasion, T. cruzi resides temporarily in an acidic vacuole which is lysed by a not well-understood mechanism. Transmission electron microscopy was used to describe the process of T. cruzi escape from the parasitophorous vacuole over the time. Using HeLa (non-professional phagocytic cells) as host cell, we observed that recently internalized parasites reside in a membrane-bounded vacuole. A few hours later, the first sign of vacuole disruption appeared as membrane discontinuities. This observation was followed by a progressive vacuole swelling as evidenced by an electron-lucent halo between the parasite and the vacuole membrane. Apparently, the vacuole membrane remnants reorganized as small vesicles that eventually disappeared from the vicinity of the parasites. Finally, parasites reach the host cell cytosol where replication takes place. The thorough ultrastructural description of this process set the base for a comprehensive understanding of the parasite-host cell interaction and, thus open the possibility of new therapeutic intervention strategies.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95263
Cueto, Juan Agustin; Santos Barrias, Emile; de Souza, Wanderley; Romano, Patricia Silvia; Trypanosoma cruzi invasion in non-phagocytic cells: an ultrastructural study; Instituto de Histología y Embriología "Dr. Mario Burgos"; Biocell; 42; 3; 12-2018; 105-108
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95263
identifier_str_mv Cueto, Juan Agustin; Santos Barrias, Emile; de Souza, Wanderley; Romano, Patricia Silvia; Trypanosoma cruzi invasion in non-phagocytic cells: an ultrastructural study; Instituto de Histología y Embriología "Dr. Mario Burgos"; Biocell; 42; 3; 12-2018; 105-108
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://biocell.institute/index.php/BioCell/article/view/105-108
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Instituto de Histología y Embriología "Dr. Mario Burgos"
publisher.none.fl_str_mv Instituto de Histología y Embriología "Dr. Mario Burgos"
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613113571180544
score 13.070432

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