Quantitative assessment of the nanoanatomy of the contractile vacuole complex in Trypanosoma cruzi

Autores
Augusto, Ingrid; Girard Dias, Wendell; Schoijet, Alejandra Cecilia; Alonso, Guillermo Daniel; Portugal, Rodrigo V.; de Souza, Wanderley; Jimenez, Veronica; Miranda, Kildare
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trypanosoma cruzi uses various mechanisms to cope with osmotic fluctuations during infection, including the remodeling of organelles such as the contractile vacuole complex (CVC). Little is known about the morphological changes of the CVC during pulsation cycles occurring upon osmotic stress. Here, we investigated the structure-function relationship between the CVC and the flagellar pocket domain where fluid discharge takes place-the adhesion plaque-during the CVC pulsation cycle. Using TcrPDEC2 and TcVps34 overexpressing mutants, known to have low and high efficiency for osmotic responses, we described a structural phenotype for the CVC that matches their corresponding physiological responses. Quantitative tomography provided data on the volume of the CVC and spongiome connections. Changes in the adhesion plaque during the pulsation cycle were also quantified and a dense filamentous network was observed. Together, the results suggest that the adhesion plaque mediates fluid discharge from the central vacuole, revealing new aspects of the osmoregulatory system in T. cruzi.
Fil: Augusto, Ingrid. Universidade Federal do Rio de Janeiro; Brasil
Fil: Girard Dias, Wendell. Universidade Federal do Rio de Janeiro; Brasil. Ministerio de Salud de Brasil. Fundación Oswaldo Cruz. Instituto Oswaldo Cruz;
Fil: Schoijet, Alejandra Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Alonso, Guillermo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Portugal, Rodrigo V.. Universidad Federal do Abc; Brasil
Fil: de Souza, Wanderley. Universidade Federal do Rio de Janeiro; Brasil. Universidade do Estado do Amazonas; Brasil
Fil: Jimenez, Veronica. California State University. College Of Natural Science And Mathematics; Estados Unidos
Fil: Miranda, Kildare. Universidade Federal do Rio de Janeiro; Brasil. Universidade do Estado do Amazonas; Brasil
Materia
COMPLEJO DE LA VACUOLA CONTRACTIL
OSMORREGULACION
TRYPANOSOMA CRUZI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/245906
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/245906
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Quantitative assessment of the nanoanatomy of the contractile vacuole complex in Trypanosoma cruziAugusto, IngridGirard Dias, WendellSchoijet, Alejandra CeciliaAlonso, Guillermo DanielPortugal, Rodrigo V.de Souza, WanderleyJimenez, VeronicaMiranda, KildareCOMPLEJO DE LA VACUOLA CONTRACTILOSMORREGULACIONTRYPANOSOMA CRUZIhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Trypanosoma cruzi uses various mechanisms to cope with osmotic fluctuations during infection, including the remodeling of organelles such as the contractile vacuole complex (CVC). Little is known about the morphological changes of the CVC during pulsation cycles occurring upon osmotic stress. Here, we investigated the structure-function relationship between the CVC and the flagellar pocket domain where fluid discharge takes place-the adhesion plaque-during the CVC pulsation cycle. Using TcrPDEC2 and TcVps34 overexpressing mutants, known to have low and high efficiency for osmotic responses, we described a structural phenotype for the CVC that matches their corresponding physiological responses. Quantitative tomography provided data on the volume of the CVC and spongiome connections. Changes in the adhesion plaque during the pulsation cycle were also quantified and a dense filamentous network was observed. Together, the results suggest that the adhesion plaque mediates fluid discharge from the central vacuole, revealing new aspects of the osmoregulatory system in T. cruzi.Fil: Augusto, Ingrid. Universidade Federal do Rio de Janeiro; BrasilFil: Girard Dias, Wendell. Universidade Federal do Rio de Janeiro; Brasil. Ministerio de Salud de Brasil. Fundación Oswaldo Cruz. Instituto Oswaldo Cruz;Fil: Schoijet, Alejandra Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Alonso, Guillermo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Portugal, Rodrigo V.. Universidad Federal do Abc; BrasilFil: de Souza, Wanderley. Universidade Federal do Rio de Janeiro; Brasil. Universidade do Estado do Amazonas; BrasilFil: Jimenez, Veronica. California State University. College Of Natural Science And Mathematics; Estados UnidosFil: Miranda, Kildare. Universidade Federal do Rio de Janeiro; Brasil. Universidade do Estado do Amazonas; BrasilLife Science Alliance2024-07-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/245906Augusto, Ingrid; Girard Dias, Wendell; Schoijet, Alejandra Cecilia; Alonso, Guillermo Daniel; Portugal, Rodrigo V.; et al.; Quantitative assessment of the nanoanatomy of the contractile vacuole complex in Trypanosoma cruzi; Life Science Alliance; Life Science Alliance; 7; 10; 29-7-2024; 1-182575-1077CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.life-science-alliance.org/content/7/10/e202402826info:eu-repo/semantics/altIdentifier/doi/10.26508/lsa.202402826info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:25:01Zoai:ri.conicet.gov.ar:11336/245906instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:25:01.367CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Quantitative assessment of the nanoanatomy of the contractile vacuole complex in Trypanosoma cruzi
title Quantitative assessment of the nanoanatomy of the contractile vacuole complex in Trypanosoma cruzi
spellingShingle Quantitative assessment of the nanoanatomy of the contractile vacuole complex in Trypanosoma cruzi
Augusto, Ingrid
COMPLEJO DE LA VACUOLA CONTRACTIL
OSMORREGULACION
TRYPANOSOMA CRUZI
title_short Quantitative assessment of the nanoanatomy of the contractile vacuole complex in Trypanosoma cruzi
title_full Quantitative assessment of the nanoanatomy of the contractile vacuole complex in Trypanosoma cruzi
title_fullStr Quantitative assessment of the nanoanatomy of the contractile vacuole complex in Trypanosoma cruzi
title_full_unstemmed Quantitative assessment of the nanoanatomy of the contractile vacuole complex in Trypanosoma cruzi
title_sort Quantitative assessment of the nanoanatomy of the contractile vacuole complex in Trypanosoma cruzi
dc.creator.none.fl_str_mv Augusto, Ingrid
Girard Dias, Wendell
Schoijet, Alejandra Cecilia
Alonso, Guillermo Daniel
Portugal, Rodrigo V.
de Souza, Wanderley
Jimenez, Veronica
Miranda, Kildare
author Augusto, Ingrid
author_facet Augusto, Ingrid
Girard Dias, Wendell
Schoijet, Alejandra Cecilia
Alonso, Guillermo Daniel
Portugal, Rodrigo V.
de Souza, Wanderley
Jimenez, Veronica
Miranda, Kildare
author_role author
author2 Girard Dias, Wendell
Schoijet, Alejandra Cecilia
Alonso, Guillermo Daniel
Portugal, Rodrigo V.
de Souza, Wanderley
Jimenez, Veronica
Miranda, Kildare
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv COMPLEJO DE LA VACUOLA CONTRACTIL
OSMORREGULACION
TRYPANOSOMA CRUZI
topic COMPLEJO DE LA VACUOLA CONTRACTIL
OSMORREGULACION
TRYPANOSOMA CRUZI
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Trypanosoma cruzi uses various mechanisms to cope with osmotic fluctuations during infection, including the remodeling of organelles such as the contractile vacuole complex (CVC). Little is known about the morphological changes of the CVC during pulsation cycles occurring upon osmotic stress. Here, we investigated the structure-function relationship between the CVC and the flagellar pocket domain where fluid discharge takes place-the adhesion plaque-during the CVC pulsation cycle. Using TcrPDEC2 and TcVps34 overexpressing mutants, known to have low and high efficiency for osmotic responses, we described a structural phenotype for the CVC that matches their corresponding physiological responses. Quantitative tomography provided data on the volume of the CVC and spongiome connections. Changes in the adhesion plaque during the pulsation cycle were also quantified and a dense filamentous network was observed. Together, the results suggest that the adhesion plaque mediates fluid discharge from the central vacuole, revealing new aspects of the osmoregulatory system in T. cruzi.
Fil: Augusto, Ingrid. Universidade Federal do Rio de Janeiro; Brasil
Fil: Girard Dias, Wendell. Universidade Federal do Rio de Janeiro; Brasil. Ministerio de Salud de Brasil. Fundación Oswaldo Cruz. Instituto Oswaldo Cruz;
Fil: Schoijet, Alejandra Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Alonso, Guillermo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Portugal, Rodrigo V.. Universidad Federal do Abc; Brasil
Fil: de Souza, Wanderley. Universidade Federal do Rio de Janeiro; Brasil. Universidade do Estado do Amazonas; Brasil
Fil: Jimenez, Veronica. California State University. College Of Natural Science And Mathematics; Estados Unidos
Fil: Miranda, Kildare. Universidade Federal do Rio de Janeiro; Brasil. Universidade do Estado do Amazonas; Brasil
description Trypanosoma cruzi uses various mechanisms to cope with osmotic fluctuations during infection, including the remodeling of organelles such as the contractile vacuole complex (CVC). Little is known about the morphological changes of the CVC during pulsation cycles occurring upon osmotic stress. Here, we investigated the structure-function relationship between the CVC and the flagellar pocket domain where fluid discharge takes place-the adhesion plaque-during the CVC pulsation cycle. Using TcrPDEC2 and TcVps34 overexpressing mutants, known to have low and high efficiency for osmotic responses, we described a structural phenotype for the CVC that matches their corresponding physiological responses. Quantitative tomography provided data on the volume of the CVC and spongiome connections. Changes in the adhesion plaque during the pulsation cycle were also quantified and a dense filamentous network was observed. Together, the results suggest that the adhesion plaque mediates fluid discharge from the central vacuole, revealing new aspects of the osmoregulatory system in T. cruzi.
publishDate 2024
dc.date.none.fl_str_mv 2024-07-29
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/245906
Augusto, Ingrid; Girard Dias, Wendell; Schoijet, Alejandra Cecilia; Alonso, Guillermo Daniel; Portugal, Rodrigo V.; et al.; Quantitative assessment of the nanoanatomy of the contractile vacuole complex in Trypanosoma cruzi; Life Science Alliance; Life Science Alliance; 7; 10; 29-7-2024; 1-18
2575-1077
CONICET Digital
CONICET
url http://hdl.handle.net/11336/245906
identifier_str_mv Augusto, Ingrid; Girard Dias, Wendell; Schoijet, Alejandra Cecilia; Alonso, Guillermo Daniel; Portugal, Rodrigo V.; et al.; Quantitative assessment of the nanoanatomy of the contractile vacuole complex in Trypanosoma cruzi; Life Science Alliance; Life Science Alliance; 7; 10; 29-7-2024; 1-18
2575-1077
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.life-science-alliance.org/content/7/10/e202402826
info:eu-repo/semantics/altIdentifier/doi/10.26508/lsa.202402826
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Life Science Alliance
publisher.none.fl_str_mv Life Science Alliance
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614248111538176
score 13.070432

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