pDNA capture using grafted adsorbents

Autores
Singh, Naveen Kumar; Dsouza, Roy N.; Yelemane, Vikas; Nentwig, Nina; Grasselli, Mariano; Fernández Lahore, Marcelo
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
BACKGROUND: ‘Expanded’ composite materials are of interest as an alternative, or as a supplement, to packed-bed chromatography during bioproduct recovery and purification. Functionalized non-woven fabrics and mega-porous bodies are examples of systems that showed promise. However, there is scarce information on their suitability to capture and release plasmid DNA (pDNA), an important type of product employed in gene therapy. RESULTS: Composite adsorbents were prepared using either chemical (CG-DEAE-NW) or gamma-irradiated graft-polymerization (GIR-DEAE-MP), and subsequently modified to have diethylamino ethanol (DEAE) functionality. Capture experiments showed that pDNA can actually reversibly bind to the two mentioned adsorbents, with capacity values of 2.4 and 1.3 mg per mL, respectively. These values are in the range of what can be expected from commercial beaded adsorbents but lower that the values expected from monoliths. CONCLUSIONS: Expanded materials, due to their high voidage, may present limited capacity for pDNA. However, such materials are able to bind proteins and other contaminants from bacterial lysate, opening the way for their utilization in the ‘negative’ mode.
Fil: Singh, Naveen Kumar. University of Notre Dame; Estados Unidos. Jacobs University; Alemania
Fil: Dsouza, Roy N.. Jacobs University; Alemania
Fil: Yelemane, Vikas. Jacobs University; Alemania
Fil: Nentwig, Nina. Jacobs University; Alemania
Fil: Grasselli, Mariano. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Fernández Lahore, Marcelo. Jacobs University; Alemania
Materia
EXPANDED ADSORBENTS
DNA PURIFICATION
GENE THERAPY
CHROMATOGRAPHY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/100077
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/100077
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling pDNA capture using grafted adsorbentsSingh, Naveen KumarDsouza, Roy N.Yelemane, VikasNentwig, NinaGrasselli, MarianoFernández Lahore, MarceloEXPANDED ADSORBENTSDNA PURIFICATIONGENE THERAPYCHROMATOGRAPHYhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2BACKGROUND: ‘Expanded’ composite materials are of interest as an alternative, or as a supplement, to packed-bed chromatography during bioproduct recovery and purification. Functionalized non-woven fabrics and mega-porous bodies are examples of systems that showed promise. However, there is scarce information on their suitability to capture and release plasmid DNA (pDNA), an important type of product employed in gene therapy. RESULTS: Composite adsorbents were prepared using either chemical (CG-DEAE-NW) or gamma-irradiated graft-polymerization (GIR-DEAE-MP), and subsequently modified to have diethylamino ethanol (DEAE) functionality. Capture experiments showed that pDNA can actually reversibly bind to the two mentioned adsorbents, with capacity values of 2.4 and 1.3 mg per mL, respectively. These values are in the range of what can be expected from commercial beaded adsorbents but lower that the values expected from monoliths. CONCLUSIONS: Expanded materials, due to their high voidage, may present limited capacity for pDNA. However, such materials are able to bind proteins and other contaminants from bacterial lysate, opening the way for their utilization in the ‘negative’ mode.Fil: Singh, Naveen Kumar. University of Notre Dame; Estados Unidos. Jacobs University; AlemaniaFil: Dsouza, Roy N.. Jacobs University; AlemaniaFil: Yelemane, Vikas. Jacobs University; AlemaniaFil: Nentwig, Nina. Jacobs University; AlemaniaFil: Grasselli, Mariano. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Fernández Lahore, Marcelo. Jacobs University; AlemaniaJohn Wiley & Sons Ltd2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/100077Singh, Naveen Kumar; Dsouza, Roy N.; Yelemane, Vikas; Nentwig, Nina; Grasselli, Mariano; et al.; pDNA capture using grafted adsorbents; John Wiley & Sons Ltd; Journal of Chemical Technology and Biotechnology; 93; 7; 7-2018; 1975-19790268-2575CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jctb.5671info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jctb.5671info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:19:51Zoai:ri.conicet.gov.ar:11336/100077instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:19:52.276CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv pDNA capture using grafted adsorbents
title pDNA capture using grafted adsorbents
spellingShingle pDNA capture using grafted adsorbents
Singh, Naveen Kumar
EXPANDED ADSORBENTS
DNA PURIFICATION
GENE THERAPY
CHROMATOGRAPHY
title_short pDNA capture using grafted adsorbents
title_full pDNA capture using grafted adsorbents
title_fullStr pDNA capture using grafted adsorbents
title_full_unstemmed pDNA capture using grafted adsorbents
title_sort pDNA capture using grafted adsorbents
dc.creator.none.fl_str_mv Singh, Naveen Kumar
Dsouza, Roy N.
Yelemane, Vikas
Nentwig, Nina
Grasselli, Mariano
Fernández Lahore, Marcelo
author Singh, Naveen Kumar
author_facet Singh, Naveen Kumar
Dsouza, Roy N.
Yelemane, Vikas
Nentwig, Nina
Grasselli, Mariano
Fernández Lahore, Marcelo
author_role author
author2 Dsouza, Roy N.
Yelemane, Vikas
Nentwig, Nina
Grasselli, Mariano
Fernández Lahore, Marcelo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv EXPANDED ADSORBENTS
DNA PURIFICATION
GENE THERAPY
CHROMATOGRAPHY
topic EXPANDED ADSORBENTS
DNA PURIFICATION
GENE THERAPY
CHROMATOGRAPHY
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv BACKGROUND: ‘Expanded’ composite materials are of interest as an alternative, or as a supplement, to packed-bed chromatography during bioproduct recovery and purification. Functionalized non-woven fabrics and mega-porous bodies are examples of systems that showed promise. However, there is scarce information on their suitability to capture and release plasmid DNA (pDNA), an important type of product employed in gene therapy. RESULTS: Composite adsorbents were prepared using either chemical (CG-DEAE-NW) or gamma-irradiated graft-polymerization (GIR-DEAE-MP), and subsequently modified to have diethylamino ethanol (DEAE) functionality. Capture experiments showed that pDNA can actually reversibly bind to the two mentioned adsorbents, with capacity values of 2.4 and 1.3 mg per mL, respectively. These values are in the range of what can be expected from commercial beaded adsorbents but lower that the values expected from monoliths. CONCLUSIONS: Expanded materials, due to their high voidage, may present limited capacity for pDNA. However, such materials are able to bind proteins and other contaminants from bacterial lysate, opening the way for their utilization in the ‘negative’ mode.
Fil: Singh, Naveen Kumar. University of Notre Dame; Estados Unidos. Jacobs University; Alemania
Fil: Dsouza, Roy N.. Jacobs University; Alemania
Fil: Yelemane, Vikas. Jacobs University; Alemania
Fil: Nentwig, Nina. Jacobs University; Alemania
Fil: Grasselli, Mariano. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Fernández Lahore, Marcelo. Jacobs University; Alemania
description BACKGROUND: ‘Expanded’ composite materials are of interest as an alternative, or as a supplement, to packed-bed chromatography during bioproduct recovery and purification. Functionalized non-woven fabrics and mega-porous bodies are examples of systems that showed promise. However, there is scarce information on their suitability to capture and release plasmid DNA (pDNA), an important type of product employed in gene therapy. RESULTS: Composite adsorbents were prepared using either chemical (CG-DEAE-NW) or gamma-irradiated graft-polymerization (GIR-DEAE-MP), and subsequently modified to have diethylamino ethanol (DEAE) functionality. Capture experiments showed that pDNA can actually reversibly bind to the two mentioned adsorbents, with capacity values of 2.4 and 1.3 mg per mL, respectively. These values are in the range of what can be expected from commercial beaded adsorbents but lower that the values expected from monoliths. CONCLUSIONS: Expanded materials, due to their high voidage, may present limited capacity for pDNA. However, such materials are able to bind proteins and other contaminants from bacterial lysate, opening the way for their utilization in the ‘negative’ mode.
publishDate 2018
dc.date.none.fl_str_mv 2018-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/100077
Singh, Naveen Kumar; Dsouza, Roy N.; Yelemane, Vikas; Nentwig, Nina; Grasselli, Mariano; et al.; pDNA capture using grafted adsorbents; John Wiley & Sons Ltd; Journal of Chemical Technology and Biotechnology; 93; 7; 7-2018; 1975-1979
0268-2575
CONICET Digital
CONICET
url http://hdl.handle.net/11336/100077
identifier_str_mv Singh, Naveen Kumar; Dsouza, Roy N.; Yelemane, Vikas; Nentwig, Nina; Grasselli, Mariano; et al.; pDNA capture using grafted adsorbents; John Wiley & Sons Ltd; Journal of Chemical Technology and Biotechnology; 93; 7; 7-2018; 1975-1979
0268-2575
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/jctb.5671
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jctb.5671
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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