High capacity cryogel-type adsorbents for protein purification

Autores
Singh, Naveen Kumar; Dsouza, Roy N.; Grasselli, Mariano; Fernández Lahore, Marcelo
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cryogel bodies were modified to obtain epoxy groups by graft-copolymerization using both chemical and gamma irradiation initiation techniques. The free epoxy adsorbents were reacted further to introduce diethylaminoethanol (DEAE) functionalities. The resulting weak anion-exchange cryogel adsorbents showed dynamic binding capacities of ca. 27 ± 3 mg/mL, which was significantly higher than previously reported for this type of adsorbent material. Gamma irradiated grafting initiation showed a 4-fold higher capacity for proteins than chemical grafting initiation procedures. The phosphate capacity for these DEAE cryogels was 119 mmol/L and also showed similar column efficiency as compared to commercial adsorbents. The large pores in the cryogel structure ensure convective transport of the molecules to active binding sites located on the polymer-grafted surface of cryogels. However, as cryogels have relatively large pores (10–100 μm), the BET area available for surface activation is low, and consequently, the capacity of the cryogels is relatively low for biomolecules, especially when compared to commercial beaded adsorbents. Nevertheless, we have shown that gamma ray mediated surface grafting of cryogel matrices greatly enhance their functional and adsorptive properties.
Fil: Singh, Naveen Kumar. Universitat Bremen. School of Enigineerring and Science Jacobs; Alemania
Fil: Dsouza, Roy N.. Universitat Bremen. School of Enigineerring and Science Jacobs; Alemania
Fil: Grasselli, Mariano. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández Lahore, Marcelo. Universitat Bremen. School of Enigineerring and Science Jacobs; Alemania
Materia
PROTEIN CHROMATOGRAPHY
MONOLITHS
MEGAPOROUS CRYOGELS
WEAK ANION-EXCHANGE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31452
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/31452
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling High capacity cryogel-type adsorbents for protein purificationSingh, Naveen KumarDsouza, Roy N.Grasselli, MarianoFernández Lahore, MarceloPROTEIN CHROMATOGRAPHYMONOLITHSMEGAPOROUS CRYOGELSWEAK ANION-EXCHANGEhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Cryogel bodies were modified to obtain epoxy groups by graft-copolymerization using both chemical and gamma irradiation initiation techniques. The free epoxy adsorbents were reacted further to introduce diethylaminoethanol (DEAE) functionalities. The resulting weak anion-exchange cryogel adsorbents showed dynamic binding capacities of ca. 27 ± 3 mg/mL, which was significantly higher than previously reported for this type of adsorbent material. Gamma irradiated grafting initiation showed a 4-fold higher capacity for proteins than chemical grafting initiation procedures. The phosphate capacity for these DEAE cryogels was 119 mmol/L and also showed similar column efficiency as compared to commercial adsorbents. The large pores in the cryogel structure ensure convective transport of the molecules to active binding sites located on the polymer-grafted surface of cryogels. However, as cryogels have relatively large pores (10–100 μm), the BET area available for surface activation is low, and consequently, the capacity of the cryogels is relatively low for biomolecules, especially when compared to commercial beaded adsorbents. Nevertheless, we have shown that gamma ray mediated surface grafting of cryogel matrices greatly enhance their functional and adsorptive properties.Fil: Singh, Naveen Kumar. Universitat Bremen. School of Enigineerring and Science Jacobs; AlemaniaFil: Dsouza, Roy N.. Universitat Bremen. School of Enigineerring and Science Jacobs; AlemaniaFil: Grasselli, Mariano. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernández Lahore, Marcelo. Universitat Bremen. School of Enigineerring and Science Jacobs; AlemaniaElsevier Science2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31452Fernández Lahore, Marcelo; Grasselli, Mariano; Dsouza, Roy N.; Singh, Naveen Kumar; High capacity cryogel-type adsorbents for protein purification; Elsevier Science; Journal of Chromatography - A; 1355; 6-2014; 143-1480021-9673CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.chroma.2014.06.008info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0021967314008899info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:02:50Zoai:ri.conicet.gov.ar:11336/31452instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:02:51.09CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv High capacity cryogel-type adsorbents for protein purification
title High capacity cryogel-type adsorbents for protein purification
spellingShingle High capacity cryogel-type adsorbents for protein purification
Singh, Naveen Kumar
PROTEIN CHROMATOGRAPHY
MONOLITHS
MEGAPOROUS CRYOGELS
WEAK ANION-EXCHANGE
title_short High capacity cryogel-type adsorbents for protein purification
title_full High capacity cryogel-type adsorbents for protein purification
title_fullStr High capacity cryogel-type adsorbents for protein purification
title_full_unstemmed High capacity cryogel-type adsorbents for protein purification
title_sort High capacity cryogel-type adsorbents for protein purification
dc.creator.none.fl_str_mv Singh, Naveen Kumar
Dsouza, Roy N.
Grasselli, Mariano
Fernández Lahore, Marcelo
author Singh, Naveen Kumar
author_facet Singh, Naveen Kumar
Dsouza, Roy N.
Grasselli, Mariano
Fernández Lahore, Marcelo
author_role author
author2 Dsouza, Roy N.
Grasselli, Mariano
Fernández Lahore, Marcelo
author2_role author
author
author
dc.subject.none.fl_str_mv PROTEIN CHROMATOGRAPHY
MONOLITHS
MEGAPOROUS CRYOGELS
WEAK ANION-EXCHANGE
topic PROTEIN CHROMATOGRAPHY
MONOLITHS
MEGAPOROUS CRYOGELS
WEAK ANION-EXCHANGE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Cryogel bodies were modified to obtain epoxy groups by graft-copolymerization using both chemical and gamma irradiation initiation techniques. The free epoxy adsorbents were reacted further to introduce diethylaminoethanol (DEAE) functionalities. The resulting weak anion-exchange cryogel adsorbents showed dynamic binding capacities of ca. 27 ± 3 mg/mL, which was significantly higher than previously reported for this type of adsorbent material. Gamma irradiated grafting initiation showed a 4-fold higher capacity for proteins than chemical grafting initiation procedures. The phosphate capacity for these DEAE cryogels was 119 mmol/L and also showed similar column efficiency as compared to commercial adsorbents. The large pores in the cryogel structure ensure convective transport of the molecules to active binding sites located on the polymer-grafted surface of cryogels. However, as cryogels have relatively large pores (10–100 μm), the BET area available for surface activation is low, and consequently, the capacity of the cryogels is relatively low for biomolecules, especially when compared to commercial beaded adsorbents. Nevertheless, we have shown that gamma ray mediated surface grafting of cryogel matrices greatly enhance their functional and adsorptive properties.
Fil: Singh, Naveen Kumar. Universitat Bremen. School of Enigineerring and Science Jacobs; Alemania
Fil: Dsouza, Roy N.. Universitat Bremen. School of Enigineerring and Science Jacobs; Alemania
Fil: Grasselli, Mariano. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández Lahore, Marcelo. Universitat Bremen. School of Enigineerring and Science Jacobs; Alemania
description Cryogel bodies were modified to obtain epoxy groups by graft-copolymerization using both chemical and gamma irradiation initiation techniques. The free epoxy adsorbents were reacted further to introduce diethylaminoethanol (DEAE) functionalities. The resulting weak anion-exchange cryogel adsorbents showed dynamic binding capacities of ca. 27 ± 3 mg/mL, which was significantly higher than previously reported for this type of adsorbent material. Gamma irradiated grafting initiation showed a 4-fold higher capacity for proteins than chemical grafting initiation procedures. The phosphate capacity for these DEAE cryogels was 119 mmol/L and also showed similar column efficiency as compared to commercial adsorbents. The large pores in the cryogel structure ensure convective transport of the molecules to active binding sites located on the polymer-grafted surface of cryogels. However, as cryogels have relatively large pores (10–100 μm), the BET area available for surface activation is low, and consequently, the capacity of the cryogels is relatively low for biomolecules, especially when compared to commercial beaded adsorbents. Nevertheless, we have shown that gamma ray mediated surface grafting of cryogel matrices greatly enhance their functional and adsorptive properties.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31452
Fernández Lahore, Marcelo; Grasselli, Mariano; Dsouza, Roy N.; Singh, Naveen Kumar; High capacity cryogel-type adsorbents for protein purification; Elsevier Science; Journal of Chromatography - A; 1355; 6-2014; 143-148
0021-9673
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31452
identifier_str_mv Fernández Lahore, Marcelo; Grasselli, Mariano; Dsouza, Roy N.; Singh, Naveen Kumar; High capacity cryogel-type adsorbents for protein purification; Elsevier Science; Journal of Chromatography - A; 1355; 6-2014; 143-148
0021-9673
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chroma.2014.06.008
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0021967314008899
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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