Enhanced anticancer activity of encapsulated geraniol into biocompatible lipid nanoparticles against A549 human lung cancer cells

Autores
Rodenak Kladniew, Boris Emilio; Gambaro, Rocío Celeste; Cisneros, José Sebastián; Huck Iriart, Cristián; Padula, Gisel; Castro, Guillermo Raul; Chain, Cecilia Yamil; Islan, German Abel
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Geraniol (GOH) is a linear monoterpene alcohol found in essential oils of plants and herbs with multiple pharmacological properties, including anticancer activity. Due to its poor water solubility and volatility, GOH stability, administration, and bioavailability in physiological conditions should be improved to develop efficient carrier systems. Thus, GOH was encapsulated into nanostructured lipid carriers (NLC) as a novel strategy to improve its anticancer activity. Highly stable and lowly dispersed GOH-loaded NLC (NLC/GOH); 110 nm mean size, polydispersity index ([PDI] < 0.2), Zeta potential around −10mV, and 95% GOH encapsulation efficiency were obtained. After GOH inclusion, the crystallographic structure of NLC changed according to wide-and small-angle X-ray scattering studies. An increased in vitro GOH release from NLC at acidic pH (tumoral environment) after 24 h was observed. The NLC/GOH proved to be safe against blood components, adsorbed in vitro a thin layer of serum proteins, and preferentially interacted with human serum albumin compared with plasmatic opsonins. An efficient time-dependent cellular uptake of NLC/GOH was observed in A549 cells after 24 h. The A549 cell viability loss was increased (1.8–3.2 fold) after GOH encapsulation into NLC, which also enhanced mitochondrial membrane depolarization and cell death. Highly cytotoxic concentrations of NLC/GOH against A549 cells resulted non-toxic to normal lung fibroblasts WI-38 cells. Finally, GOH nanoencapsulation into NLC potentiated the inhibition of A549 cell migration exerted by free GOH.
Fil: Rodenak Kladniew, Boris Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Gambaro, Rocío Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Cisneros, José Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Huck Iriart, Cristián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Tecnologías Emergentes y Ciencias Aplicadas. - Universidad Nacional de San Martin. Instituto de Tecnologías Emergentes y Ciencias Aplicadas; Argentina
Fil: Padula, Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Castro, Guillermo Raul. Laboratorio Max Planck de Biologia Estructural, Quimica y Biofisica Molecular de Rosario ; Centro de Estudios Interdisciplinarios ; Universidad Nacional de Rosario; . Universidad Federal do Abc; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chain, Cecilia Yamil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Islan, German Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Materia
CELL MIGRATION
CYTOTOXICITY
DRUG DELIVERY
GERANIOL
LUNG CANCER CELLS
NANOSTRUCTURED LIPID CARRIERS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/226938
Acceder
id CONICETDig_ab5a6067173a2c5e2b2eb3d55fd56310
oai_identifier_str oai:ri.conicet.gov.ar:11336/226938
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Enhanced anticancer activity of encapsulated geraniol into biocompatible lipid nanoparticles against A549 human lung cancer cellsRodenak Kladniew, Boris EmilioGambaro, Rocío CelesteCisneros, José SebastiánHuck Iriart, CristiánPadula, GiselCastro, Guillermo RaulChain, Cecilia YamilIslan, German AbelCELL MIGRATIONCYTOTOXICITYDRUG DELIVERYGERANIOLLUNG CANCER CELLSNANOSTRUCTURED LIPID CARRIERShttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Geraniol (GOH) is a linear monoterpene alcohol found in essential oils of plants and herbs with multiple pharmacological properties, including anticancer activity. Due to its poor water solubility and volatility, GOH stability, administration, and bioavailability in physiological conditions should be improved to develop efficient carrier systems. Thus, GOH was encapsulated into nanostructured lipid carriers (NLC) as a novel strategy to improve its anticancer activity. Highly stable and lowly dispersed GOH-loaded NLC (NLC/GOH); 110 nm mean size, polydispersity index ([PDI] < 0.2), Zeta potential around −10mV, and 95% GOH encapsulation efficiency were obtained. After GOH inclusion, the crystallographic structure of NLC changed according to wide-and small-angle X-ray scattering studies. An increased in vitro GOH release from NLC at acidic pH (tumoral environment) after 24 h was observed. The NLC/GOH proved to be safe against blood components, adsorbed in vitro a thin layer of serum proteins, and preferentially interacted with human serum albumin compared with plasmatic opsonins. An efficient time-dependent cellular uptake of NLC/GOH was observed in A549 cells after 24 h. The A549 cell viability loss was increased (1.8–3.2 fold) after GOH encapsulation into NLC, which also enhanced mitochondrial membrane depolarization and cell death. Highly cytotoxic concentrations of NLC/GOH against A549 cells resulted non-toxic to normal lung fibroblasts WI-38 cells. Finally, GOH nanoencapsulation into NLC potentiated the inhibition of A549 cell migration exerted by free GOH.Fil: Rodenak Kladniew, Boris Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Gambaro, Rocío Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Cisneros, José Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Huck Iriart, Cristián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Tecnologías Emergentes y Ciencias Aplicadas. - Universidad Nacional de San Martin. Instituto de Tecnologías Emergentes y Ciencias Aplicadas; ArgentinaFil: Padula, Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Castro, Guillermo Raul. Laboratorio Max Planck de Biologia Estructural, Quimica y Biofisica Molecular de Rosario ; Centro de Estudios Interdisciplinarios ; Universidad Nacional de Rosario; . Universidad Federal do Abc; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chain, Cecilia Yamil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Islan, German Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaElsevier2023-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/226938Rodenak Kladniew, Boris Emilio; Gambaro, Rocío Celeste; Cisneros, José Sebastián; Huck Iriart, Cristián; Padula, Gisel; et al.; Enhanced anticancer activity of encapsulated geraniol into biocompatible lipid nanoparticles against A549 human lung cancer cells; Elsevier; Journal of Drug Delivery Science and Technology; 80; 2-2023; 104159-1041701773-2247CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1773224723000114?via%3Dihubinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jddst.2023.104159info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:16:38Zoai:ri.conicet.gov.ar:11336/226938instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:16:38.369CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enhanced anticancer activity of encapsulated geraniol into biocompatible lipid nanoparticles against A549 human lung cancer cells
title Enhanced anticancer activity of encapsulated geraniol into biocompatible lipid nanoparticles against A549 human lung cancer cells
spellingShingle Enhanced anticancer activity of encapsulated geraniol into biocompatible lipid nanoparticles against A549 human lung cancer cells
Rodenak Kladniew, Boris Emilio
CELL MIGRATION
CYTOTOXICITY
DRUG DELIVERY
GERANIOL
LUNG CANCER CELLS
NANOSTRUCTURED LIPID CARRIERS
title_short Enhanced anticancer activity of encapsulated geraniol into biocompatible lipid nanoparticles against A549 human lung cancer cells
title_full Enhanced anticancer activity of encapsulated geraniol into biocompatible lipid nanoparticles against A549 human lung cancer cells
title_fullStr Enhanced anticancer activity of encapsulated geraniol into biocompatible lipid nanoparticles against A549 human lung cancer cells
title_full_unstemmed Enhanced anticancer activity of encapsulated geraniol into biocompatible lipid nanoparticles against A549 human lung cancer cells
title_sort Enhanced anticancer activity of encapsulated geraniol into biocompatible lipid nanoparticles against A549 human lung cancer cells
dc.creator.none.fl_str_mv Rodenak Kladniew, Boris Emilio
Gambaro, Rocío Celeste
Cisneros, José Sebastián
Huck Iriart, Cristián
Padula, Gisel
Castro, Guillermo Raul
Chain, Cecilia Yamil
Islan, German Abel
author Rodenak Kladniew, Boris Emilio
author_facet Rodenak Kladniew, Boris Emilio
Gambaro, Rocío Celeste
Cisneros, José Sebastián
Huck Iriart, Cristián
Padula, Gisel
Castro, Guillermo Raul
Chain, Cecilia Yamil
Islan, German Abel
author_role author
author2 Gambaro, Rocío Celeste
Cisneros, José Sebastián
Huck Iriart, Cristián
Padula, Gisel
Castro, Guillermo Raul
Chain, Cecilia Yamil
Islan, German Abel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CELL MIGRATION
CYTOTOXICITY
DRUG DELIVERY
GERANIOL
LUNG CANCER CELLS
NANOSTRUCTURED LIPID CARRIERS
topic CELL MIGRATION
CYTOTOXICITY
DRUG DELIVERY
GERANIOL
LUNG CANCER CELLS
NANOSTRUCTURED LIPID CARRIERS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Geraniol (GOH) is a linear monoterpene alcohol found in essential oils of plants and herbs with multiple pharmacological properties, including anticancer activity. Due to its poor water solubility and volatility, GOH stability, administration, and bioavailability in physiological conditions should be improved to develop efficient carrier systems. Thus, GOH was encapsulated into nanostructured lipid carriers (NLC) as a novel strategy to improve its anticancer activity. Highly stable and lowly dispersed GOH-loaded NLC (NLC/GOH); 110 nm mean size, polydispersity index ([PDI] < 0.2), Zeta potential around −10mV, and 95% GOH encapsulation efficiency were obtained. After GOH inclusion, the crystallographic structure of NLC changed according to wide-and small-angle X-ray scattering studies. An increased in vitro GOH release from NLC at acidic pH (tumoral environment) after 24 h was observed. The NLC/GOH proved to be safe against blood components, adsorbed in vitro a thin layer of serum proteins, and preferentially interacted with human serum albumin compared with plasmatic opsonins. An efficient time-dependent cellular uptake of NLC/GOH was observed in A549 cells after 24 h. The A549 cell viability loss was increased (1.8–3.2 fold) after GOH encapsulation into NLC, which also enhanced mitochondrial membrane depolarization and cell death. Highly cytotoxic concentrations of NLC/GOH against A549 cells resulted non-toxic to normal lung fibroblasts WI-38 cells. Finally, GOH nanoencapsulation into NLC potentiated the inhibition of A549 cell migration exerted by free GOH.
Fil: Rodenak Kladniew, Boris Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Gambaro, Rocío Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Cisneros, José Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Huck Iriart, Cristián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Tecnologías Emergentes y Ciencias Aplicadas. - Universidad Nacional de San Martin. Instituto de Tecnologías Emergentes y Ciencias Aplicadas; Argentina
Fil: Padula, Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Castro, Guillermo Raul. Laboratorio Max Planck de Biologia Estructural, Quimica y Biofisica Molecular de Rosario ; Centro de Estudios Interdisciplinarios ; Universidad Nacional de Rosario; . Universidad Federal do Abc; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chain, Cecilia Yamil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Islan, German Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
description Geraniol (GOH) is a linear monoterpene alcohol found in essential oils of plants and herbs with multiple pharmacological properties, including anticancer activity. Due to its poor water solubility and volatility, GOH stability, administration, and bioavailability in physiological conditions should be improved to develop efficient carrier systems. Thus, GOH was encapsulated into nanostructured lipid carriers (NLC) as a novel strategy to improve its anticancer activity. Highly stable and lowly dispersed GOH-loaded NLC (NLC/GOH); 110 nm mean size, polydispersity index ([PDI] < 0.2), Zeta potential around −10mV, and 95% GOH encapsulation efficiency were obtained. After GOH inclusion, the crystallographic structure of NLC changed according to wide-and small-angle X-ray scattering studies. An increased in vitro GOH release from NLC at acidic pH (tumoral environment) after 24 h was observed. The NLC/GOH proved to be safe against blood components, adsorbed in vitro a thin layer of serum proteins, and preferentially interacted with human serum albumin compared with plasmatic opsonins. An efficient time-dependent cellular uptake of NLC/GOH was observed in A549 cells after 24 h. The A549 cell viability loss was increased (1.8–3.2 fold) after GOH encapsulation into NLC, which also enhanced mitochondrial membrane depolarization and cell death. Highly cytotoxic concentrations of NLC/GOH against A549 cells resulted non-toxic to normal lung fibroblasts WI-38 cells. Finally, GOH nanoencapsulation into NLC potentiated the inhibition of A549 cell migration exerted by free GOH.
publishDate 2023
dc.date.none.fl_str_mv 2023-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/226938
Rodenak Kladniew, Boris Emilio; Gambaro, Rocío Celeste; Cisneros, José Sebastián; Huck Iriart, Cristián; Padula, Gisel; et al.; Enhanced anticancer activity of encapsulated geraniol into biocompatible lipid nanoparticles against A549 human lung cancer cells; Elsevier; Journal of Drug Delivery Science and Technology; 80; 2-2023; 104159-104170
1773-2247
CONICET Digital
CONICET
url http://hdl.handle.net/11336/226938
identifier_str_mv Rodenak Kladniew, Boris Emilio; Gambaro, Rocío Celeste; Cisneros, José Sebastián; Huck Iriart, Cristián; Padula, Gisel; et al.; Enhanced anticancer activity of encapsulated geraniol into biocompatible lipid nanoparticles against A549 human lung cancer cells; Elsevier; Journal of Drug Delivery Science and Technology; 80; 2-2023; 104159-104170
1773-2247
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1773224723000114?via%3Dihub
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jddst.2023.104159
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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