Involvement of hydrogen peroxide in histamine-induced modulation of WM35 human malignant melanoma cell proliferation

Autores
Medina, Vanina Araceli; Massari, Noelia Andrea; Cricco, Graciela Patricia; Martín, Gabriela A.; Bergoc, Rosa Maria; Rivera, Elena Susana
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Histamine is a recognized growth factor in melanoma, and exogenous histamine produces a dual effect on proliferation. We have previously reported that histamine at micromolar concentrations reduces the proliferation of melanoma cell lines. To investigate the mechanism by which histamine inhibits proliferation of WM35 human melanoma cells, we have studied the involvement of histamine in reactive oxygen species production and antioxidant enzyme regulation in these cells. Results indicate that histamine treatment (10 μM) significantly increased hydrogen peroxide levels, whereas it slightly decreased superoxide levels associated with an enhancement of superoxide dismutase and a reduction in catalase activity. Additionally, catalase treatment reversed the inhibitory effect of histamine on proliferation, and various treatments that reduce hydrogen peroxide formation increased proliferation of these cells. Furthermore, we demonstrate that the inhibition of proliferation produced by histamine was mediated at least in part by an induction of cell senescence. We conclude that hydrogen peroxide is involved in histamine-mediated modulation of proliferation in malignant melanoma cells.
Fil: Medina, Vanina Araceli. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Massari, Noelia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Cricco, Graciela Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Martín, Gabriela A.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Bergoc, Rosa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Rivera, Elena Susana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Materia
ANTIOXIDANT ENZYMES
CELL PROLIFERATION
FREE RADICALS
HISTAMINE
MELANOMA
REACTIVE OXYGEN SPECIES
SENESCENCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/114226
Acceder
id CONICETDig_f2c10aa9147b7beac4a82b0ed531d76b
oai_identifier_str oai:ri.conicet.gov.ar:11336/114226
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Involvement of hydrogen peroxide in histamine-induced modulation of WM35 human malignant melanoma cell proliferationMedina, Vanina AraceliMassari, Noelia AndreaCricco, Graciela PatriciaMartín, Gabriela A.Bergoc, Rosa MariaRivera, Elena SusanaANTIOXIDANT ENZYMESCELL PROLIFERATIONFREE RADICALSHISTAMINEMELANOMAREACTIVE OXYGEN SPECIESSENESCENCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Histamine is a recognized growth factor in melanoma, and exogenous histamine produces a dual effect on proliferation. We have previously reported that histamine at micromolar concentrations reduces the proliferation of melanoma cell lines. To investigate the mechanism by which histamine inhibits proliferation of WM35 human melanoma cells, we have studied the involvement of histamine in reactive oxygen species production and antioxidant enzyme regulation in these cells. Results indicate that histamine treatment (10 μM) significantly increased hydrogen peroxide levels, whereas it slightly decreased superoxide levels associated with an enhancement of superoxide dismutase and a reduction in catalase activity. Additionally, catalase treatment reversed the inhibitory effect of histamine on proliferation, and various treatments that reduce hydrogen peroxide formation increased proliferation of these cells. Furthermore, we demonstrate that the inhibition of proliferation produced by histamine was mediated at least in part by an induction of cell senescence. We conclude that hydrogen peroxide is involved in histamine-mediated modulation of proliferation in malignant melanoma cells.Fil: Medina, Vanina Araceli. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Massari, Noelia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Cricco, Graciela Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Martín, Gabriela A.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Bergoc, Rosa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Rivera, Elena Susana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaElsevier Science Inc2009-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/114226Medina, Vanina Araceli; Massari, Noelia Andrea; Cricco, Graciela Patricia; Martín, Gabriela A.; Bergoc, Rosa Maria; et al.; Involvement of hydrogen peroxide in histamine-induced modulation of WM35 human malignant melanoma cell proliferation; Elsevier Science Inc; Free Radical Biology and Medicine; 46; 11; 6-2009; 1510-15150891-5849CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0891584909001269info:eu-repo/semantics/altIdentifier/doi/10.1016/j.freeradbiomed.2009.03.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:58:44Zoai:ri.conicet.gov.ar:11336/114226instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:58:44.878CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Involvement of hydrogen peroxide in histamine-induced modulation of WM35 human malignant melanoma cell proliferation
title Involvement of hydrogen peroxide in histamine-induced modulation of WM35 human malignant melanoma cell proliferation
spellingShingle Involvement of hydrogen peroxide in histamine-induced modulation of WM35 human malignant melanoma cell proliferation
Medina, Vanina Araceli
ANTIOXIDANT ENZYMES
CELL PROLIFERATION
FREE RADICALS
HISTAMINE
MELANOMA
REACTIVE OXYGEN SPECIES
SENESCENCE
title_short Involvement of hydrogen peroxide in histamine-induced modulation of WM35 human malignant melanoma cell proliferation
title_full Involvement of hydrogen peroxide in histamine-induced modulation of WM35 human malignant melanoma cell proliferation
title_fullStr Involvement of hydrogen peroxide in histamine-induced modulation of WM35 human malignant melanoma cell proliferation
title_full_unstemmed Involvement of hydrogen peroxide in histamine-induced modulation of WM35 human malignant melanoma cell proliferation
title_sort Involvement of hydrogen peroxide in histamine-induced modulation of WM35 human malignant melanoma cell proliferation
dc.creator.none.fl_str_mv Medina, Vanina Araceli
Massari, Noelia Andrea
Cricco, Graciela Patricia
Martín, Gabriela A.
Bergoc, Rosa Maria
Rivera, Elena Susana
author Medina, Vanina Araceli
author_facet Medina, Vanina Araceli
Massari, Noelia Andrea
Cricco, Graciela Patricia
Martín, Gabriela A.
Bergoc, Rosa Maria
Rivera, Elena Susana
author_role author
author2 Massari, Noelia Andrea
Cricco, Graciela Patricia
Martín, Gabriela A.
Bergoc, Rosa Maria
Rivera, Elena Susana
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ANTIOXIDANT ENZYMES
CELL PROLIFERATION
FREE RADICALS
HISTAMINE
MELANOMA
REACTIVE OXYGEN SPECIES
SENESCENCE
topic ANTIOXIDANT ENZYMES
CELL PROLIFERATION
FREE RADICALS
HISTAMINE
MELANOMA
REACTIVE OXYGEN SPECIES
SENESCENCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Histamine is a recognized growth factor in melanoma, and exogenous histamine produces a dual effect on proliferation. We have previously reported that histamine at micromolar concentrations reduces the proliferation of melanoma cell lines. To investigate the mechanism by which histamine inhibits proliferation of WM35 human melanoma cells, we have studied the involvement of histamine in reactive oxygen species production and antioxidant enzyme regulation in these cells. Results indicate that histamine treatment (10 μM) significantly increased hydrogen peroxide levels, whereas it slightly decreased superoxide levels associated with an enhancement of superoxide dismutase and a reduction in catalase activity. Additionally, catalase treatment reversed the inhibitory effect of histamine on proliferation, and various treatments that reduce hydrogen peroxide formation increased proliferation of these cells. Furthermore, we demonstrate that the inhibition of proliferation produced by histamine was mediated at least in part by an induction of cell senescence. We conclude that hydrogen peroxide is involved in histamine-mediated modulation of proliferation in malignant melanoma cells.
Fil: Medina, Vanina Araceli. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Massari, Noelia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Cricco, Graciela Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Martín, Gabriela A.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Bergoc, Rosa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Rivera, Elena Susana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
description Histamine is a recognized growth factor in melanoma, and exogenous histamine produces a dual effect on proliferation. We have previously reported that histamine at micromolar concentrations reduces the proliferation of melanoma cell lines. To investigate the mechanism by which histamine inhibits proliferation of WM35 human melanoma cells, we have studied the involvement of histamine in reactive oxygen species production and antioxidant enzyme regulation in these cells. Results indicate that histamine treatment (10 μM) significantly increased hydrogen peroxide levels, whereas it slightly decreased superoxide levels associated with an enhancement of superoxide dismutase and a reduction in catalase activity. Additionally, catalase treatment reversed the inhibitory effect of histamine on proliferation, and various treatments that reduce hydrogen peroxide formation increased proliferation of these cells. Furthermore, we demonstrate that the inhibition of proliferation produced by histamine was mediated at least in part by an induction of cell senescence. We conclude that hydrogen peroxide is involved in histamine-mediated modulation of proliferation in malignant melanoma cells.
publishDate 2009
dc.date.none.fl_str_mv 2009-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/114226
Medina, Vanina Araceli; Massari, Noelia Andrea; Cricco, Graciela Patricia; Martín, Gabriela A.; Bergoc, Rosa Maria; et al.; Involvement of hydrogen peroxide in histamine-induced modulation of WM35 human malignant melanoma cell proliferation; Elsevier Science Inc; Free Radical Biology and Medicine; 46; 11; 6-2009; 1510-1515
0891-5849
CONICET Digital
CONICET
url http://hdl.handle.net/11336/114226
identifier_str_mv Medina, Vanina Araceli; Massari, Noelia Andrea; Cricco, Graciela Patricia; Martín, Gabriela A.; Bergoc, Rosa Maria; et al.; Involvement of hydrogen peroxide in histamine-induced modulation of WM35 human malignant melanoma cell proliferation; Elsevier Science Inc; Free Radical Biology and Medicine; 46; 11; 6-2009; 1510-1515
0891-5849
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0891584909001269
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.freeradbiomed.2009.03.003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083128241684480
score 12.891075

Publicaciones similares