Role of H4 receptor in histamine-mediated responses in human melanoma

Autores
Massari, Noelia Andrea; Medina, Vanina Araceli; Martinel Lamas, Diego José; Cricco, Graciela; Croci, Máximo; Sambuco, Lorena Andrea; Bergoc, Rosa Maria; Rivera, Elena
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We have previously reported that histamine at micromolar concentrations reduces the proliferation of melanoma cell lines. It is also known that melanoma cells express histamine H1, H2 and H3 receptors. The aim of this work was to investigate the presence of histamine H4 receptor (H4R) in human melanoma cells and its associated biological processes. In order to better understand the importance of histamine in tumor development, we explored the expression of H4R in human melanoma tissue biopsies. The expression of H4R in WM35 and M1/15 cells was analyzed by RT-PCR, Western blot and immunocytochemistry. To characterize the biological responses we evaluated cell proliferation by clonogenic assay and BrdU incorporation. In addition, cell senescence and differentiation were determined by β-galactosidase enzyme assay and dopa oxidase activity, respectively. The expression levels of H4R were determined by immunohistochemistry in 19 samples of human malignant lesions. Results indicate that melanoma cells express H4R at the mRNA and protein level. By using histamine agonists, antagonists and H4 siRNA we showed that the inhibitory effect of histamine on proliferation was in part mediated through the stimulation of the H4R. The decrease in proliferation was associated with an induction of cell senescence and an increase in melanogenesis that is a differentiation marker of these cells. Furthermore, H4R was expressed in 42% of human melanoma biopses. To our knowledge this is the first report that describes the presence of the H4R in melanoma cells and tissue suggesting a potential therapeutic application of H4R ligands.
Fil: Massari, Noelia Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Medina, Vanina Araceli. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Martinel Lamas, Diego José. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Cricco, Graciela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Croci, Máximo. Instituto de Inmunooncología; Argentina
Fil: Sambuco, Lorena Andrea. Instituto de Inmunooncología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Bergoc, Rosa Maria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Rivera, Elena. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Materia
Histamine
Human Melanoma
Histamine H4 Receptor
Cell Proliferation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13376
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/13376
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of H4 receptor in histamine-mediated responses in human melanomaMassari, Noelia AndreaMedina, Vanina AraceliMartinel Lamas, Diego JoséCricco, GracielaCroci, MáximoSambuco, Lorena AndreaBergoc, Rosa MariaRivera, ElenaHistamineHuman MelanomaHistamine H4 ReceptorCell Proliferationhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3We have previously reported that histamine at micromolar concentrations reduces the proliferation of melanoma cell lines. It is also known that melanoma cells express histamine H1, H2 and H3 receptors. The aim of this work was to investigate the presence of histamine H4 receptor (H4R) in human melanoma cells and its associated biological processes. In order to better understand the importance of histamine in tumor development, we explored the expression of H4R in human melanoma tissue biopsies. The expression of H4R in WM35 and M1/15 cells was analyzed by RT-PCR, Western blot and immunocytochemistry. To characterize the biological responses we evaluated cell proliferation by clonogenic assay and BrdU incorporation. In addition, cell senescence and differentiation were determined by β-galactosidase enzyme assay and dopa oxidase activity, respectively. The expression levels of H4R were determined by immunohistochemistry in 19 samples of human malignant lesions. Results indicate that melanoma cells express H4R at the mRNA and protein level. By using histamine agonists, antagonists and H4 siRNA we showed that the inhibitory effect of histamine on proliferation was in part mediated through the stimulation of the H4R. The decrease in proliferation was associated with an induction of cell senescence and an increase in melanogenesis that is a differentiation marker of these cells. Furthermore, H4R was expressed in 42% of human melanoma biopses. To our knowledge this is the first report that describes the presence of the H4R in melanoma cells and tissue suggesting a potential therapeutic application of H4R ligands.Fil: Massari, Noelia Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Medina, Vanina Araceli. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Martinel Lamas, Diego José. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Cricco, Graciela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Croci, Máximo. Instituto de Inmunooncología; ArgentinaFil: Sambuco, Lorena Andrea. Instituto de Inmunooncología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Bergoc, Rosa Maria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Rivera, Elena. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaLippincott Williams & Wilkins2011-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13376Massari, Noelia Andrea; Medina, Vanina Araceli; Martinel Lamas, Diego José; Cricco, Graciela; Croci, Máximo; et al.; Role of H4 receptor in histamine-mediated responses in human melanoma ; Lippincott Williams & Wilkins; Melanoma Research; 21; 5; 10-2011; 395-4040960-8931enginfo:eu-repo/semantics/altIdentifier/url/http://journals.lww.com/melanomaresearch/pages/articleviewer.aspx?year=2011&issue=10000&article=00004&type=abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1097/CMR.0b013e328347ee53info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:03Zoai:ri.conicet.gov.ar:11336/13376instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:03.624CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of H4 receptor in histamine-mediated responses in human melanoma
title Role of H4 receptor in histamine-mediated responses in human melanoma
spellingShingle Role of H4 receptor in histamine-mediated responses in human melanoma
Massari, Noelia Andrea
Histamine
Human Melanoma
Histamine H4 Receptor
Cell Proliferation
title_short Role of H4 receptor in histamine-mediated responses in human melanoma
title_full Role of H4 receptor in histamine-mediated responses in human melanoma
title_fullStr Role of H4 receptor in histamine-mediated responses in human melanoma
title_full_unstemmed Role of H4 receptor in histamine-mediated responses in human melanoma
title_sort Role of H4 receptor in histamine-mediated responses in human melanoma
dc.creator.none.fl_str_mv Massari, Noelia Andrea
Medina, Vanina Araceli
Martinel Lamas, Diego José
Cricco, Graciela
Croci, Máximo
Sambuco, Lorena Andrea
Bergoc, Rosa Maria
Rivera, Elena
author Massari, Noelia Andrea
author_facet Massari, Noelia Andrea
Medina, Vanina Araceli
Martinel Lamas, Diego José
Cricco, Graciela
Croci, Máximo
Sambuco, Lorena Andrea
Bergoc, Rosa Maria
Rivera, Elena
author_role author
author2 Medina, Vanina Araceli
Martinel Lamas, Diego José
Cricco, Graciela
Croci, Máximo
Sambuco, Lorena Andrea
Bergoc, Rosa Maria
Rivera, Elena
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Histamine
Human Melanoma
Histamine H4 Receptor
Cell Proliferation
topic Histamine
Human Melanoma
Histamine H4 Receptor
Cell Proliferation
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv We have previously reported that histamine at micromolar concentrations reduces the proliferation of melanoma cell lines. It is also known that melanoma cells express histamine H1, H2 and H3 receptors. The aim of this work was to investigate the presence of histamine H4 receptor (H4R) in human melanoma cells and its associated biological processes. In order to better understand the importance of histamine in tumor development, we explored the expression of H4R in human melanoma tissue biopsies. The expression of H4R in WM35 and M1/15 cells was analyzed by RT-PCR, Western blot and immunocytochemistry. To characterize the biological responses we evaluated cell proliferation by clonogenic assay and BrdU incorporation. In addition, cell senescence and differentiation were determined by β-galactosidase enzyme assay and dopa oxidase activity, respectively. The expression levels of H4R were determined by immunohistochemistry in 19 samples of human malignant lesions. Results indicate that melanoma cells express H4R at the mRNA and protein level. By using histamine agonists, antagonists and H4 siRNA we showed that the inhibitory effect of histamine on proliferation was in part mediated through the stimulation of the H4R. The decrease in proliferation was associated with an induction of cell senescence and an increase in melanogenesis that is a differentiation marker of these cells. Furthermore, H4R was expressed in 42% of human melanoma biopses. To our knowledge this is the first report that describes the presence of the H4R in melanoma cells and tissue suggesting a potential therapeutic application of H4R ligands.
Fil: Massari, Noelia Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Medina, Vanina Araceli. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Martinel Lamas, Diego José. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Cricco, Graciela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Croci, Máximo. Instituto de Inmunooncología; Argentina
Fil: Sambuco, Lorena Andrea. Instituto de Inmunooncología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Bergoc, Rosa Maria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Rivera, Elena. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
description We have previously reported that histamine at micromolar concentrations reduces the proliferation of melanoma cell lines. It is also known that melanoma cells express histamine H1, H2 and H3 receptors. The aim of this work was to investigate the presence of histamine H4 receptor (H4R) in human melanoma cells and its associated biological processes. In order to better understand the importance of histamine in tumor development, we explored the expression of H4R in human melanoma tissue biopsies. The expression of H4R in WM35 and M1/15 cells was analyzed by RT-PCR, Western blot and immunocytochemistry. To characterize the biological responses we evaluated cell proliferation by clonogenic assay and BrdU incorporation. In addition, cell senescence and differentiation were determined by β-galactosidase enzyme assay and dopa oxidase activity, respectively. The expression levels of H4R were determined by immunohistochemistry in 19 samples of human malignant lesions. Results indicate that melanoma cells express H4R at the mRNA and protein level. By using histamine agonists, antagonists and H4 siRNA we showed that the inhibitory effect of histamine on proliferation was in part mediated through the stimulation of the H4R. The decrease in proliferation was associated with an induction of cell senescence and an increase in melanogenesis that is a differentiation marker of these cells. Furthermore, H4R was expressed in 42% of human melanoma biopses. To our knowledge this is the first report that describes the presence of the H4R in melanoma cells and tissue suggesting a potential therapeutic application of H4R ligands.
publishDate 2011
dc.date.none.fl_str_mv 2011-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13376
Massari, Noelia Andrea; Medina, Vanina Araceli; Martinel Lamas, Diego José; Cricco, Graciela; Croci, Máximo; et al.; Role of H4 receptor in histamine-mediated responses in human melanoma ; Lippincott Williams & Wilkins; Melanoma Research; 21; 5; 10-2011; 395-404
0960-8931
url http://hdl.handle.net/11336/13376
identifier_str_mv Massari, Noelia Andrea; Medina, Vanina Araceli; Martinel Lamas, Diego José; Cricco, Graciela; Croci, Máximo; et al.; Role of H4 receptor in histamine-mediated responses in human melanoma ; Lippincott Williams & Wilkins; Melanoma Research; 21; 5; 10-2011; 395-404
0960-8931
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.lww.com/melanomaresearch/pages/articleviewer.aspx?year=2011&issue=10000&article=00004&type=abstract
info:eu-repo/semantics/altIdentifier/doi/10.1097/CMR.0b013e328347ee53
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Lippincott Williams & Wilkins
publisher.none.fl_str_mv Lippincott Williams & Wilkins
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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