Intracellular calcium mobilization: A key step for chitosan-induced anthraquinone production in Rubia tinctorum L.

Autores
Vasconsuelo, Andrea Anahi; Morelli, Susana Ana; Picotto, Gabriela; Giulietti, Ana Maria; Boland, Ricardo Leopoldo
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Elicitation with chitosan (200 mg/l) significantly stimulates (∼100%) anthraquinone (Aq) synthesis in Rubia tinctorum L. cultures, but the mechanism of elicitation is largely unknown. We recently showed that the effects of the elicitor involve phospholipase C (PLC), protein kinase C (PKC), phosphoinositide 3-kinase (PI3K) and mitogen activated protein kinase (MAPK) cascades. Here, we show that the elicitor action on Aq production can be blocked with the intracellular Ca2+ chelator BAPTA-AM but not in a Ca 2+-free medium (+EGTA) or in the presence of the voltage-dependent Ca2+ channel antagonists verapamil and nifedipine. In agreement with these observations, spectrofluorimetric measurements in Fura 2-loaded R. tinctorum cells show that chitosan increases intracellular Ca2+ concentration in a medium devoid of calcium. Short treatment intervals (1-5 min) of cells with the elicitor significantly increased DAG and IP3 formation. Moreover, the PI-PLC inhibitors neomycin and U73122 diminished to a great extent chitosan-induced Aq synthesis. Blockers of Ca2+ release from inner stores such as 2-APB, TMB-8, caffeine, ruthenium red and dantrolene inhibited elicitation. Chitosan rapidly stimulated (phosphorylated) MAPK. This effect was significantly decreased by the calcium modulators used above. However, EGTA did not prevent activation of MAPK. Compound LY24009, a blocker of PI3K, inhibited MAPK phosphorylation by chitosan. Accordingly, an increase in PI3K activity was observed in parallel. The results of this study show that chitosan induction of anthraquinone synthesis in R. tinctorum involves the stimulation of PLC, intracellular Ca2+ mobilization and PI3K, which mediate MAPK activation.
Fil: Vasconsuelo, Andrea Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina
Fil: Morelli, Susana Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Picotto, Gabriela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina
Fil: Giulietti, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina
Fil: Boland, Ricardo Leopoldo. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina
Materia
ANTHRAQUINONE SYNTHESIS
CHITOSAN
DAG
INTRACELLULAR CA 2+
IP3
MAPK
PI3-K
PLC
RUBIA TINCTORUM L.
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/97827
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/97827
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Intracellular calcium mobilization: A key step for chitosan-induced anthraquinone production in Rubia tinctorum L.Vasconsuelo, Andrea AnahiMorelli, Susana AnaPicotto, GabrielaGiulietti, Ana MariaBoland, Ricardo LeopoldoANTHRAQUINONE SYNTHESISCHITOSANDAGINTRACELLULAR CA 2+IP3MAPKPI3-KPLCRUBIA TINCTORUM L.https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Elicitation with chitosan (200 mg/l) significantly stimulates (∼100%) anthraquinone (Aq) synthesis in Rubia tinctorum L. cultures, but the mechanism of elicitation is largely unknown. We recently showed that the effects of the elicitor involve phospholipase C (PLC), protein kinase C (PKC), phosphoinositide 3-kinase (PI3K) and mitogen activated protein kinase (MAPK) cascades. Here, we show that the elicitor action on Aq production can be blocked with the intracellular Ca2+ chelator BAPTA-AM but not in a Ca 2+-free medium (+EGTA) or in the presence of the voltage-dependent Ca2+ channel antagonists verapamil and nifedipine. In agreement with these observations, spectrofluorimetric measurements in Fura 2-loaded R. tinctorum cells show that chitosan increases intracellular Ca2+ concentration in a medium devoid of calcium. Short treatment intervals (1-5 min) of cells with the elicitor significantly increased DAG and IP3 formation. Moreover, the PI-PLC inhibitors neomycin and U73122 diminished to a great extent chitosan-induced Aq synthesis. Blockers of Ca2+ release from inner stores such as 2-APB, TMB-8, caffeine, ruthenium red and dantrolene inhibited elicitation. Chitosan rapidly stimulated (phosphorylated) MAPK. This effect was significantly decreased by the calcium modulators used above. However, EGTA did not prevent activation of MAPK. Compound LY24009, a blocker of PI3K, inhibited MAPK phosphorylation by chitosan. Accordingly, an increase in PI3K activity was observed in parallel. The results of this study show that chitosan induction of anthraquinone synthesis in R. tinctorum involves the stimulation of PLC, intracellular Ca2+ mobilization and PI3K, which mediate MAPK activation.Fil: Vasconsuelo, Andrea Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; ArgentinaFil: Morelli, Susana Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Picotto, Gabriela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; ArgentinaFil: Giulietti, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; ArgentinaFil: Boland, Ricardo Leopoldo. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; ArgentinaElsevier Ireland2005-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/97827Vasconsuelo, Andrea Anahi; Morelli, Susana Ana; Picotto, Gabriela; Giulietti, Ana Maria; Boland, Ricardo Leopoldo; Intracellular calcium mobilization: A key step for chitosan-induced anthraquinone production in Rubia tinctorum L.; Elsevier Ireland; Plant Science; 169; 4; 10-2005; 712-7200168-9452CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2005.05.022info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168945205001925info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:41:05Zoai:ri.conicet.gov.ar:11336/97827instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:41:05.835CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Intracellular calcium mobilization: A key step for chitosan-induced anthraquinone production in Rubia tinctorum L.
title Intracellular calcium mobilization: A key step for chitosan-induced anthraquinone production in Rubia tinctorum L.
spellingShingle Intracellular calcium mobilization: A key step for chitosan-induced anthraquinone production in Rubia tinctorum L.
Vasconsuelo, Andrea Anahi
ANTHRAQUINONE SYNTHESIS
CHITOSAN
DAG
INTRACELLULAR CA 2+
IP3
MAPK
PI3-K
PLC
RUBIA TINCTORUM L.
title_short Intracellular calcium mobilization: A key step for chitosan-induced anthraquinone production in Rubia tinctorum L.
title_full Intracellular calcium mobilization: A key step for chitosan-induced anthraquinone production in Rubia tinctorum L.
title_fullStr Intracellular calcium mobilization: A key step for chitosan-induced anthraquinone production in Rubia tinctorum L.
title_full_unstemmed Intracellular calcium mobilization: A key step for chitosan-induced anthraquinone production in Rubia tinctorum L.
title_sort Intracellular calcium mobilization: A key step for chitosan-induced anthraquinone production in Rubia tinctorum L.
dc.creator.none.fl_str_mv Vasconsuelo, Andrea Anahi
Morelli, Susana Ana
Picotto, Gabriela
Giulietti, Ana Maria
Boland, Ricardo Leopoldo
author Vasconsuelo, Andrea Anahi
author_facet Vasconsuelo, Andrea Anahi
Morelli, Susana Ana
Picotto, Gabriela
Giulietti, Ana Maria
Boland, Ricardo Leopoldo
author_role author
author2 Morelli, Susana Ana
Picotto, Gabriela
Giulietti, Ana Maria
Boland, Ricardo Leopoldo
author2_role author
author
author
author
dc.subject.none.fl_str_mv ANTHRAQUINONE SYNTHESIS
CHITOSAN
DAG
INTRACELLULAR CA 2+
IP3
MAPK
PI3-K
PLC
RUBIA TINCTORUM L.
topic ANTHRAQUINONE SYNTHESIS
CHITOSAN
DAG
INTRACELLULAR CA 2+
IP3
MAPK
PI3-K
PLC
RUBIA TINCTORUM L.
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Elicitation with chitosan (200 mg/l) significantly stimulates (∼100%) anthraquinone (Aq) synthesis in Rubia tinctorum L. cultures, but the mechanism of elicitation is largely unknown. We recently showed that the effects of the elicitor involve phospholipase C (PLC), protein kinase C (PKC), phosphoinositide 3-kinase (PI3K) and mitogen activated protein kinase (MAPK) cascades. Here, we show that the elicitor action on Aq production can be blocked with the intracellular Ca2+ chelator BAPTA-AM but not in a Ca 2+-free medium (+EGTA) or in the presence of the voltage-dependent Ca2+ channel antagonists verapamil and nifedipine. In agreement with these observations, spectrofluorimetric measurements in Fura 2-loaded R. tinctorum cells show that chitosan increases intracellular Ca2+ concentration in a medium devoid of calcium. Short treatment intervals (1-5 min) of cells with the elicitor significantly increased DAG and IP3 formation. Moreover, the PI-PLC inhibitors neomycin and U73122 diminished to a great extent chitosan-induced Aq synthesis. Blockers of Ca2+ release from inner stores such as 2-APB, TMB-8, caffeine, ruthenium red and dantrolene inhibited elicitation. Chitosan rapidly stimulated (phosphorylated) MAPK. This effect was significantly decreased by the calcium modulators used above. However, EGTA did not prevent activation of MAPK. Compound LY24009, a blocker of PI3K, inhibited MAPK phosphorylation by chitosan. Accordingly, an increase in PI3K activity was observed in parallel. The results of this study show that chitosan induction of anthraquinone synthesis in R. tinctorum involves the stimulation of PLC, intracellular Ca2+ mobilization and PI3K, which mediate MAPK activation.
Fil: Vasconsuelo, Andrea Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina
Fil: Morelli, Susana Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Picotto, Gabriela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina
Fil: Giulietti, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina
Fil: Boland, Ricardo Leopoldo. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina
description Elicitation with chitosan (200 mg/l) significantly stimulates (∼100%) anthraquinone (Aq) synthesis in Rubia tinctorum L. cultures, but the mechanism of elicitation is largely unknown. We recently showed that the effects of the elicitor involve phospholipase C (PLC), protein kinase C (PKC), phosphoinositide 3-kinase (PI3K) and mitogen activated protein kinase (MAPK) cascades. Here, we show that the elicitor action on Aq production can be blocked with the intracellular Ca2+ chelator BAPTA-AM but not in a Ca 2+-free medium (+EGTA) or in the presence of the voltage-dependent Ca2+ channel antagonists verapamil and nifedipine. In agreement with these observations, spectrofluorimetric measurements in Fura 2-loaded R. tinctorum cells show that chitosan increases intracellular Ca2+ concentration in a medium devoid of calcium. Short treatment intervals (1-5 min) of cells with the elicitor significantly increased DAG and IP3 formation. Moreover, the PI-PLC inhibitors neomycin and U73122 diminished to a great extent chitosan-induced Aq synthesis. Blockers of Ca2+ release from inner stores such as 2-APB, TMB-8, caffeine, ruthenium red and dantrolene inhibited elicitation. Chitosan rapidly stimulated (phosphorylated) MAPK. This effect was significantly decreased by the calcium modulators used above. However, EGTA did not prevent activation of MAPK. Compound LY24009, a blocker of PI3K, inhibited MAPK phosphorylation by chitosan. Accordingly, an increase in PI3K activity was observed in parallel. The results of this study show that chitosan induction of anthraquinone synthesis in R. tinctorum involves the stimulation of PLC, intracellular Ca2+ mobilization and PI3K, which mediate MAPK activation.
publishDate 2005
dc.date.none.fl_str_mv 2005-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/97827
Vasconsuelo, Andrea Anahi; Morelli, Susana Ana; Picotto, Gabriela; Giulietti, Ana Maria; Boland, Ricardo Leopoldo; Intracellular calcium mobilization: A key step for chitosan-induced anthraquinone production in Rubia tinctorum L.; Elsevier Ireland; Plant Science; 169; 4; 10-2005; 712-720
0168-9452
CONICET Digital
CONICET
url http://hdl.handle.net/11336/97827
identifier_str_mv Vasconsuelo, Andrea Anahi; Morelli, Susana Ana; Picotto, Gabriela; Giulietti, Ana Maria; Boland, Ricardo Leopoldo; Intracellular calcium mobilization: A key step for chitosan-induced anthraquinone production in Rubia tinctorum L.; Elsevier Ireland; Plant Science; 169; 4; 10-2005; 712-720
0168-9452
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2005.05.022
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168945205001925
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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