Anthraquinones production in Rubia tinctorum cell suspension cultures: Down scale of shear effects

Autores
Busto, Víctor Daniel; Calabró López, Roberto Ariel; Rodriguez Talou, Julian; Giulietti, Ana Maria; Merchuk, José C.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effect of turbulence on suspended cells is one of the most complex problems in the scale-up of cell cultures. In the present paper, a direct comparison of the effects of turbulence on suspension cultures of Rubia tinctorum in a standard bioreactor and in shake flask cultures was done. A procedure derived from the well known global method proposed by Nishikawa et al. (1977) was applied. Standard flasks and four-baffled shake flasks were used. The effect of turbulence and light irradiation on cell viability, biomass, and anthraquinones (AQs) production was evaluated. The biomass concentration and AQs production obtained using baffled shake flasks agitated at 360 rpm were similar to that achieved in R. tinctorum suspension cultures growing in a stirred tank bioreactor operating at 450 rpm, previously published (Busto et al., 2008). The effect of light on AQs production was found to be very significant, and a difference of up to 48% was found in cells with and without illumination after 7 days of culture. It is concluded that this down-scaled and simple flask culture system is a suitable and valid small scale instrument for the study of intracellular mechanisms of turbulence-induced AQs production in R. tinctorum suspension cultures.
Fil: Busto, Víctor Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Biotecnología; Argentina
Fil: Calabró López, Roberto Ariel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Biotecnología; Argentina
Fil: Rodriguez Talou, Julian. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Biotecnología; Argentina
Fil: Giulietti, Ana Maria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina
Fil: Merchuk, José C.. Ben Gurion University of The Negev; Israel;
Materia
Anthraquinones
Shear Stress
Turbulence
Scale-Down
Rubia Tinctorum
Plant Cell Cultures
Shaken Bioreactors
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1907
Acceder
id CONICETDig_0276677d3f8daf1ff9e9bb5e69e53c8e
oai_identifier_str oai:ri.conicet.gov.ar:11336/1907
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Anthraquinones production in Rubia tinctorum cell suspension cultures: Down scale of shear effectsBusto, Víctor DanielCalabró López, Roberto ArielRodriguez Talou, JulianGiulietti, Ana MariaMerchuk, José C.AnthraquinonesShear StressTurbulenceScale-DownRubia TinctorumPlant Cell CulturesShaken Bioreactorshttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The effect of turbulence on suspended cells is one of the most complex problems in the scale-up of cell cultures. In the present paper, a direct comparison of the effects of turbulence on suspension cultures of Rubia tinctorum in a standard bioreactor and in shake flask cultures was done. A procedure derived from the well known global method proposed by Nishikawa et al. (1977) was applied. Standard flasks and four-baffled shake flasks were used. The effect of turbulence and light irradiation on cell viability, biomass, and anthraquinones (AQs) production was evaluated. The biomass concentration and AQs production obtained using baffled shake flasks agitated at 360 rpm were similar to that achieved in R. tinctorum suspension cultures growing in a stirred tank bioreactor operating at 450 rpm, previously published (Busto et al., 2008). The effect of light on AQs production was found to be very significant, and a difference of up to 48% was found in cells with and without illumination after 7 days of culture. It is concluded that this down-scaled and simple flask culture system is a suitable and valid small scale instrument for the study of intracellular mechanisms of turbulence-induced AQs production in R. tinctorum suspension cultures.Fil: Busto, Víctor Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Biotecnología; ArgentinaFil: Calabró López, Roberto Ariel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Biotecnología; ArgentinaFil: Rodriguez Talou, Julian. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Biotecnología; ArgentinaFil: Giulietti, Ana Maria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; ArgentinaFil: Merchuk, José C.. Ben Gurion University of The Negev; Israel;Elsevier2013-08-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1907Busto, Víctor Daniel; Calabró López, Roberto Ariel; Rodriguez Talou, Julian; Giulietti, Ana Maria; Merchuk, José C.; Anthraquinones production in Rubia tinctorum cell suspension cultures: Down scale of shear effects; Elsevier; Biochemical Engineering Journal; 77; 15-8-2013; 119-1281369-703Xenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1369703X13001630info:eu-repo/semantics/altIdentifier/doi/doi:10.1016/j.bej.2013.05.013info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:25Zoai:ri.conicet.gov.ar:11336/1907instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:25.75CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Anthraquinones production in Rubia tinctorum cell suspension cultures: Down scale of shear effects
title Anthraquinones production in Rubia tinctorum cell suspension cultures: Down scale of shear effects
spellingShingle Anthraquinones production in Rubia tinctorum cell suspension cultures: Down scale of shear effects
Busto, Víctor Daniel
Anthraquinones
Shear Stress
Turbulence
Scale-Down
Rubia Tinctorum
Plant Cell Cultures
Shaken Bioreactors
title_short Anthraquinones production in Rubia tinctorum cell suspension cultures: Down scale of shear effects
title_full Anthraquinones production in Rubia tinctorum cell suspension cultures: Down scale of shear effects
title_fullStr Anthraquinones production in Rubia tinctorum cell suspension cultures: Down scale of shear effects
title_full_unstemmed Anthraquinones production in Rubia tinctorum cell suspension cultures: Down scale of shear effects
title_sort Anthraquinones production in Rubia tinctorum cell suspension cultures: Down scale of shear effects
dc.creator.none.fl_str_mv Busto, Víctor Daniel
Calabró López, Roberto Ariel
Rodriguez Talou, Julian
Giulietti, Ana Maria
Merchuk, José C.
author Busto, Víctor Daniel
author_facet Busto, Víctor Daniel
Calabró López, Roberto Ariel
Rodriguez Talou, Julian
Giulietti, Ana Maria
Merchuk, José C.
author_role author
author2 Calabró López, Roberto Ariel
Rodriguez Talou, Julian
Giulietti, Ana Maria
Merchuk, José C.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Anthraquinones
Shear Stress
Turbulence
Scale-Down
Rubia Tinctorum
Plant Cell Cultures
Shaken Bioreactors
topic Anthraquinones
Shear Stress
Turbulence
Scale-Down
Rubia Tinctorum
Plant Cell Cultures
Shaken Bioreactors
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The effect of turbulence on suspended cells is one of the most complex problems in the scale-up of cell cultures. In the present paper, a direct comparison of the effects of turbulence on suspension cultures of Rubia tinctorum in a standard bioreactor and in shake flask cultures was done. A procedure derived from the well known global method proposed by Nishikawa et al. (1977) was applied. Standard flasks and four-baffled shake flasks were used. The effect of turbulence and light irradiation on cell viability, biomass, and anthraquinones (AQs) production was evaluated. The biomass concentration and AQs production obtained using baffled shake flasks agitated at 360 rpm were similar to that achieved in R. tinctorum suspension cultures growing in a stirred tank bioreactor operating at 450 rpm, previously published (Busto et al., 2008). The effect of light on AQs production was found to be very significant, and a difference of up to 48% was found in cells with and without illumination after 7 days of culture. It is concluded that this down-scaled and simple flask culture system is a suitable and valid small scale instrument for the study of intracellular mechanisms of turbulence-induced AQs production in R. tinctorum suspension cultures.
Fil: Busto, Víctor Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Biotecnología; Argentina
Fil: Calabró López, Roberto Ariel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Biotecnología; Argentina
Fil: Rodriguez Talou, Julian. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Biotecnología; Argentina
Fil: Giulietti, Ana Maria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina
Fil: Merchuk, José C.. Ben Gurion University of The Negev; Israel;
description The effect of turbulence on suspended cells is one of the most complex problems in the scale-up of cell cultures. In the present paper, a direct comparison of the effects of turbulence on suspension cultures of Rubia tinctorum in a standard bioreactor and in shake flask cultures was done. A procedure derived from the well known global method proposed by Nishikawa et al. (1977) was applied. Standard flasks and four-baffled shake flasks were used. The effect of turbulence and light irradiation on cell viability, biomass, and anthraquinones (AQs) production was evaluated. The biomass concentration and AQs production obtained using baffled shake flasks agitated at 360 rpm were similar to that achieved in R. tinctorum suspension cultures growing in a stirred tank bioreactor operating at 450 rpm, previously published (Busto et al., 2008). The effect of light on AQs production was found to be very significant, and a difference of up to 48% was found in cells with and without illumination after 7 days of culture. It is concluded that this down-scaled and simple flask culture system is a suitable and valid small scale instrument for the study of intracellular mechanisms of turbulence-induced AQs production in R. tinctorum suspension cultures.
publishDate 2013
dc.date.none.fl_str_mv 2013-08-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1907
Busto, Víctor Daniel; Calabró López, Roberto Ariel; Rodriguez Talou, Julian; Giulietti, Ana Maria; Merchuk, José C.; Anthraquinones production in Rubia tinctorum cell suspension cultures: Down scale of shear effects; Elsevier; Biochemical Engineering Journal; 77; 15-8-2013; 119-128
1369-703X
url http://hdl.handle.net/11336/1907
identifier_str_mv Busto, Víctor Daniel; Calabró López, Roberto Ariel; Rodriguez Talou, Julian; Giulietti, Ana Maria; Merchuk, José C.; Anthraquinones production in Rubia tinctorum cell suspension cultures: Down scale of shear effects; Elsevier; Biochemical Engineering Journal; 77; 15-8-2013; 119-128
1369-703X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1369703X13001630
info:eu-repo/semantics/altIdentifier/doi/doi:10.1016/j.bej.2013.05.013
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.885934

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