Signal transduction events mediating chitosan stimulation of anthraquinone synthesis in Rubia tinctorum

Autores
Vasconsuelo, Andrea Anahi; Giulietti, Ana Maria; Boland, Ricardo Leopoldo
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The signalling mechanism by which chitosan increases anthraquinones in Rubia tinctorum L. is largely unknown. We recently showed that the effects of the elicitor require activation of the PLC/PKC cascade. In view of the intrincate network of pathways mediating extracellular stimuli, in this study we investigated whether mitogen activated protein kinase (MAPK), a pathway known to be a PKC target, also participates in chitosan action. Immunoblot analysis revealed a marked stimulation of the MAPK in elicited cells. In presence of the PKC inhibitor calphostin C, lack of activation of MAPK paralleled by a 90% suppression of the chitosan-dependent increase in anthraquinones were observed. Moreover, the elicitor action was decreased 65% by MAPK kinase inhibitor PD 98059. Also, we tested whether the adenylyl cyclase (AC)/cAMP/PKA messenger system plays a role in elicitation. Forskolin, which stimulates AC, and the PKA activators adenosine 3′,5′-cyclic monophosphorothioate-Sp (Sp-cAMPS) or 8-Br-cAMP, were not able to mimic the induction of anthraquinone synthesis in R. tinctorum cells by the elicitor. In addition, the PKA inhibitor adenosine 3′,5′-cyclic monophosphorothiolate-Rp (Rp-cAMPS) did not block chitosan effects. On the other hand, phosphoinositide 3′-OH-kinase (PI3K) inhibitors LY204002 and wortmannin blocked chitosan stimulation of PKC activity and anthraquinone synthesis. These results involve PI3K-mediated activation of PKC and in turn of MAPK in chitosan elicitation.
Fil: Vasconsuelo, Andrea Anahi. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Giulietti, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina
Fil: Boland, Ricardo Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Materia
ANTHRAQUINONE
CHITOSAN
PI3K/PDK-1/PKC/MAPK
RUBIA TINCTORUM L.
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/97824
Acceder
id CONICETDig_2fb131122a9ba8bfe8ed97b6fc20ecaa
oai_identifier_str oai:ri.conicet.gov.ar:11336/97824
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Signal transduction events mediating chitosan stimulation of anthraquinone synthesis in Rubia tinctorumVasconsuelo, Andrea AnahiGiulietti, Ana MariaBoland, Ricardo LeopoldoANTHRAQUINONECHITOSANPI3K/PDK-1/PKC/MAPKRUBIA TINCTORUM L.https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The signalling mechanism by which chitosan increases anthraquinones in Rubia tinctorum L. is largely unknown. We recently showed that the effects of the elicitor require activation of the PLC/PKC cascade. In view of the intrincate network of pathways mediating extracellular stimuli, in this study we investigated whether mitogen activated protein kinase (MAPK), a pathway known to be a PKC target, also participates in chitosan action. Immunoblot analysis revealed a marked stimulation of the MAPK in elicited cells. In presence of the PKC inhibitor calphostin C, lack of activation of MAPK paralleled by a 90% suppression of the chitosan-dependent increase in anthraquinones were observed. Moreover, the elicitor action was decreased 65% by MAPK kinase inhibitor PD 98059. Also, we tested whether the adenylyl cyclase (AC)/cAMP/PKA messenger system plays a role in elicitation. Forskolin, which stimulates AC, and the PKA activators adenosine 3′,5′-cyclic monophosphorothioate-Sp (Sp-cAMPS) or 8-Br-cAMP, were not able to mimic the induction of anthraquinone synthesis in R. tinctorum cells by the elicitor. In addition, the PKA inhibitor adenosine 3′,5′-cyclic monophosphorothiolate-Rp (Rp-cAMPS) did not block chitosan effects. On the other hand, phosphoinositide 3′-OH-kinase (PI3K) inhibitors LY204002 and wortmannin blocked chitosan stimulation of PKC activity and anthraquinone synthesis. These results involve PI3K-mediated activation of PKC and in turn of MAPK in chitosan elicitation.Fil: Vasconsuelo, Andrea Anahi. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Giulietti, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; ArgentinaFil: Boland, Ricardo Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaElsevier Ireland2004-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/97824Vasconsuelo, Andrea Anahi; Giulietti, Ana Maria; Boland, Ricardo Leopoldo; Signal transduction events mediating chitosan stimulation of anthraquinone synthesis in Rubia tinctorum; Elsevier Ireland; Plant Science; 166; 2; 12-2004; 405-4130168-9452CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2003.10.007info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168945203004485info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:02:26Zoai:ri.conicet.gov.ar:11336/97824instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:02:27.066CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Signal transduction events mediating chitosan stimulation of anthraquinone synthesis in Rubia tinctorum
title Signal transduction events mediating chitosan stimulation of anthraquinone synthesis in Rubia tinctorum
spellingShingle Signal transduction events mediating chitosan stimulation of anthraquinone synthesis in Rubia tinctorum
Vasconsuelo, Andrea Anahi
ANTHRAQUINONE
CHITOSAN
PI3K/PDK-1/PKC/MAPK
RUBIA TINCTORUM L.
title_short Signal transduction events mediating chitosan stimulation of anthraquinone synthesis in Rubia tinctorum
title_full Signal transduction events mediating chitosan stimulation of anthraquinone synthesis in Rubia tinctorum
title_fullStr Signal transduction events mediating chitosan stimulation of anthraquinone synthesis in Rubia tinctorum
title_full_unstemmed Signal transduction events mediating chitosan stimulation of anthraquinone synthesis in Rubia tinctorum
title_sort Signal transduction events mediating chitosan stimulation of anthraquinone synthesis in Rubia tinctorum
dc.creator.none.fl_str_mv Vasconsuelo, Andrea Anahi
Giulietti, Ana Maria
Boland, Ricardo Leopoldo
author Vasconsuelo, Andrea Anahi
author_facet Vasconsuelo, Andrea Anahi
Giulietti, Ana Maria
Boland, Ricardo Leopoldo
author_role author
author2 Giulietti, Ana Maria
Boland, Ricardo Leopoldo
author2_role author
author
dc.subject.none.fl_str_mv ANTHRAQUINONE
CHITOSAN
PI3K/PDK-1/PKC/MAPK
RUBIA TINCTORUM L.
topic ANTHRAQUINONE
CHITOSAN
PI3K/PDK-1/PKC/MAPK
RUBIA TINCTORUM L.
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The signalling mechanism by which chitosan increases anthraquinones in Rubia tinctorum L. is largely unknown. We recently showed that the effects of the elicitor require activation of the PLC/PKC cascade. In view of the intrincate network of pathways mediating extracellular stimuli, in this study we investigated whether mitogen activated protein kinase (MAPK), a pathway known to be a PKC target, also participates in chitosan action. Immunoblot analysis revealed a marked stimulation of the MAPK in elicited cells. In presence of the PKC inhibitor calphostin C, lack of activation of MAPK paralleled by a 90% suppression of the chitosan-dependent increase in anthraquinones were observed. Moreover, the elicitor action was decreased 65% by MAPK kinase inhibitor PD 98059. Also, we tested whether the adenylyl cyclase (AC)/cAMP/PKA messenger system plays a role in elicitation. Forskolin, which stimulates AC, and the PKA activators adenosine 3′,5′-cyclic monophosphorothioate-Sp (Sp-cAMPS) or 8-Br-cAMP, were not able to mimic the induction of anthraquinone synthesis in R. tinctorum cells by the elicitor. In addition, the PKA inhibitor adenosine 3′,5′-cyclic monophosphorothiolate-Rp (Rp-cAMPS) did not block chitosan effects. On the other hand, phosphoinositide 3′-OH-kinase (PI3K) inhibitors LY204002 and wortmannin blocked chitosan stimulation of PKC activity and anthraquinone synthesis. These results involve PI3K-mediated activation of PKC and in turn of MAPK in chitosan elicitation.
Fil: Vasconsuelo, Andrea Anahi. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Giulietti, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina
Fil: Boland, Ricardo Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
description The signalling mechanism by which chitosan increases anthraquinones in Rubia tinctorum L. is largely unknown. We recently showed that the effects of the elicitor require activation of the PLC/PKC cascade. In view of the intrincate network of pathways mediating extracellular stimuli, in this study we investigated whether mitogen activated protein kinase (MAPK), a pathway known to be a PKC target, also participates in chitosan action. Immunoblot analysis revealed a marked stimulation of the MAPK in elicited cells. In presence of the PKC inhibitor calphostin C, lack of activation of MAPK paralleled by a 90% suppression of the chitosan-dependent increase in anthraquinones were observed. Moreover, the elicitor action was decreased 65% by MAPK kinase inhibitor PD 98059. Also, we tested whether the adenylyl cyclase (AC)/cAMP/PKA messenger system plays a role in elicitation. Forskolin, which stimulates AC, and the PKA activators adenosine 3′,5′-cyclic monophosphorothioate-Sp (Sp-cAMPS) or 8-Br-cAMP, were not able to mimic the induction of anthraquinone synthesis in R. tinctorum cells by the elicitor. In addition, the PKA inhibitor adenosine 3′,5′-cyclic monophosphorothiolate-Rp (Rp-cAMPS) did not block chitosan effects. On the other hand, phosphoinositide 3′-OH-kinase (PI3K) inhibitors LY204002 and wortmannin blocked chitosan stimulation of PKC activity and anthraquinone synthesis. These results involve PI3K-mediated activation of PKC and in turn of MAPK in chitosan elicitation.
publishDate 2004
dc.date.none.fl_str_mv 2004-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/97824
Vasconsuelo, Andrea Anahi; Giulietti, Ana Maria; Boland, Ricardo Leopoldo; Signal transduction events mediating chitosan stimulation of anthraquinone synthesis in Rubia tinctorum; Elsevier Ireland; Plant Science; 166; 2; 12-2004; 405-413
0168-9452
CONICET Digital
CONICET
url http://hdl.handle.net/11336/97824
identifier_str_mv Vasconsuelo, Andrea Anahi; Giulietti, Ana Maria; Boland, Ricardo Leopoldo; Signal transduction events mediating chitosan stimulation of anthraquinone synthesis in Rubia tinctorum; Elsevier Ireland; Plant Science; 166; 2; 12-2004; 405-413
0168-9452
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2003.10.007
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168945203004485
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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