Impedance spectroscopy applied to the fast wounding dynamics of an electrical wound-healing assay in mammalian cells

Autores
Bellotti, Mariela; Giana, Fabián Eduardo; Bonetto, Fabian Jose
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Electrical wound-healing assays are often used as a means to study in vitro cell migration and proliferation. In such analysis, a cell monolayer that sits on a small electrode is electrically wounded and its spectral impedance is then continuously measured in order to monitor the healing process. The relatively slow dynamics of the cell healing have been extensively studied, while those of the much faster wounding phase have not yet been investigated. An analysis of the electrical properties of a particular cell type during this phase could give extra information about the changes in the cell membrane due to the application of the wounding current, and could also be useful to optimize the wounding regime for different cell types. The main issue when trying to register information about these dynamics is that the traditional measurement scheme employed in typical wound-healing assays doesn't allow the simultaneous application of the wounding signal and measurement of the system's impedance. In this paper, we overcome this limitation by implementing a measurement strategy consisting of cycles of fast alternating low- and high-voltage signals applied on electrodes covered with mammalian cells. This approach is capable of registering the fast impedance changes during the transient regime corresponding to the cell wounding process. Furthermore, these quasi-simultaneous high- and low-voltage measurements can be compared in order to obtain an empirical correlation between both quantities.
Fil: Bellotti, Mariela. Comisión Nacional de Energía Atómica. Gerencia de Área de Energía Nuclear. Gerencia de Ingeniería Nuclear (CAB). Laboratorio Cavitación y Biotecnología; Argentina. Comisión Nacional de Energía Atómica. Gerencia del Área de Energía Nuclear. Instituto Balseiro; Argentina
Fil: Giana, Fabián Eduardo. Comisión Nacional de Energía Atómica. Gerencia de Área de Energía Nuclear. Gerencia de Ingeniería Nuclear (CAB). Laboratorio Cavitación y Biotecnología; Argentina. Comisión Nacional de Energía Atómica. Gerencia del Área de Energía Nuclear. Instituto Balseiro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina
Fil: Bonetto, Fabian Jose. Comisión Nacional de Energía Atómica. Gerencia de Área de Energía Nuclear. Gerencia de Ingeniería Nuclear (CAB). Laboratorio Cavitación y Biotecnología; Argentina. Comisión Nacional de Energía Atómica. Gerencia del Área de Energía Nuclear. Instituto Balseiro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina
Materia
CELL WOUNDING DYNAMICS
IMPEDANCE SPECTROSCOPY
MAMMALIAN CELLS
NONLINEAR VOLTAGE-CURRENT RELATIONSHIP
WOUND-HEALING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/127057
Acceder
id CONICETDig_f282ee200e4200dd79953aa98d3a56dc
oai_identifier_str oai:ri.conicet.gov.ar:11336/127057
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Impedance spectroscopy applied to the fast wounding dynamics of an electrical wound-healing assay in mammalian cellsBellotti, MarielaGiana, Fabián EduardoBonetto, Fabian JoseCELL WOUNDING DYNAMICSIMPEDANCE SPECTROSCOPYMAMMALIAN CELLSNONLINEAR VOLTAGE-CURRENT RELATIONSHIPWOUND-HEALINGhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Electrical wound-healing assays are often used as a means to study in vitro cell migration and proliferation. In such analysis, a cell monolayer that sits on a small electrode is electrically wounded and its spectral impedance is then continuously measured in order to monitor the healing process. The relatively slow dynamics of the cell healing have been extensively studied, while those of the much faster wounding phase have not yet been investigated. An analysis of the electrical properties of a particular cell type during this phase could give extra information about the changes in the cell membrane due to the application of the wounding current, and could also be useful to optimize the wounding regime for different cell types. The main issue when trying to register information about these dynamics is that the traditional measurement scheme employed in typical wound-healing assays doesn't allow the simultaneous application of the wounding signal and measurement of the system's impedance. In this paper, we overcome this limitation by implementing a measurement strategy consisting of cycles of fast alternating low- and high-voltage signals applied on electrodes covered with mammalian cells. This approach is capable of registering the fast impedance changes during the transient regime corresponding to the cell wounding process. Furthermore, these quasi-simultaneous high- and low-voltage measurements can be compared in order to obtain an empirical correlation between both quantities.Fil: Bellotti, Mariela. Comisión Nacional de Energía Atómica. Gerencia de Área de Energía Nuclear. Gerencia de Ingeniería Nuclear (CAB). Laboratorio Cavitación y Biotecnología; Argentina. Comisión Nacional de Energía Atómica. Gerencia del Área de Energía Nuclear. Instituto Balseiro; ArgentinaFil: Giana, Fabián Eduardo. Comisión Nacional de Energía Atómica. Gerencia de Área de Energía Nuclear. Gerencia de Ingeniería Nuclear (CAB). Laboratorio Cavitación y Biotecnología; Argentina. Comisión Nacional de Energía Atómica. Gerencia del Área de Energía Nuclear. Instituto Balseiro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; ArgentinaFil: Bonetto, Fabian Jose. Comisión Nacional de Energía Atómica. Gerencia de Área de Energía Nuclear. Gerencia de Ingeniería Nuclear (CAB). Laboratorio Cavitación y Biotecnología; Argentina. Comisión Nacional de Energía Atómica. Gerencia del Área de Energía Nuclear. Instituto Balseiro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; ArgentinaIOP Publishing2015-06-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/127057Bellotti, Mariela; Giana, Fabián Eduardo; Bonetto, Fabian Jose; Impedance spectroscopy applied to the fast wounding dynamics of an electrical wound-healing assay in mammalian cells; IOP Publishing; Measurement Science & Technology (print); 26; 8; 25-6-2015; 1-110957-02331361-6501CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1088/0957-0233/26/8/085701info:eu-repo/semantics/altIdentifier/url/https://iopscience.iop.org/article/10.1088/0957-0233/26/8/085701info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:10Zoai:ri.conicet.gov.ar:11336/127057instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:10.482CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Impedance spectroscopy applied to the fast wounding dynamics of an electrical wound-healing assay in mammalian cells
title Impedance spectroscopy applied to the fast wounding dynamics of an electrical wound-healing assay in mammalian cells
spellingShingle Impedance spectroscopy applied to the fast wounding dynamics of an electrical wound-healing assay in mammalian cells
Bellotti, Mariela
CELL WOUNDING DYNAMICS
IMPEDANCE SPECTROSCOPY
MAMMALIAN CELLS
NONLINEAR VOLTAGE-CURRENT RELATIONSHIP
WOUND-HEALING
title_short Impedance spectroscopy applied to the fast wounding dynamics of an electrical wound-healing assay in mammalian cells
title_full Impedance spectroscopy applied to the fast wounding dynamics of an electrical wound-healing assay in mammalian cells
title_fullStr Impedance spectroscopy applied to the fast wounding dynamics of an electrical wound-healing assay in mammalian cells
title_full_unstemmed Impedance spectroscopy applied to the fast wounding dynamics of an electrical wound-healing assay in mammalian cells
title_sort Impedance spectroscopy applied to the fast wounding dynamics of an electrical wound-healing assay in mammalian cells
dc.creator.none.fl_str_mv Bellotti, Mariela
Giana, Fabián Eduardo
Bonetto, Fabian Jose
author Bellotti, Mariela
author_facet Bellotti, Mariela
Giana, Fabián Eduardo
Bonetto, Fabian Jose
author_role author
author2 Giana, Fabián Eduardo
Bonetto, Fabian Jose
author2_role author
author
dc.subject.none.fl_str_mv CELL WOUNDING DYNAMICS
IMPEDANCE SPECTROSCOPY
MAMMALIAN CELLS
NONLINEAR VOLTAGE-CURRENT RELATIONSHIP
WOUND-HEALING
topic CELL WOUNDING DYNAMICS
IMPEDANCE SPECTROSCOPY
MAMMALIAN CELLS
NONLINEAR VOLTAGE-CURRENT RELATIONSHIP
WOUND-HEALING
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Electrical wound-healing assays are often used as a means to study in vitro cell migration and proliferation. In such analysis, a cell monolayer that sits on a small electrode is electrically wounded and its spectral impedance is then continuously measured in order to monitor the healing process. The relatively slow dynamics of the cell healing have been extensively studied, while those of the much faster wounding phase have not yet been investigated. An analysis of the electrical properties of a particular cell type during this phase could give extra information about the changes in the cell membrane due to the application of the wounding current, and could also be useful to optimize the wounding regime for different cell types. The main issue when trying to register information about these dynamics is that the traditional measurement scheme employed in typical wound-healing assays doesn't allow the simultaneous application of the wounding signal and measurement of the system's impedance. In this paper, we overcome this limitation by implementing a measurement strategy consisting of cycles of fast alternating low- and high-voltage signals applied on electrodes covered with mammalian cells. This approach is capable of registering the fast impedance changes during the transient regime corresponding to the cell wounding process. Furthermore, these quasi-simultaneous high- and low-voltage measurements can be compared in order to obtain an empirical correlation between both quantities.
Fil: Bellotti, Mariela. Comisión Nacional de Energía Atómica. Gerencia de Área de Energía Nuclear. Gerencia de Ingeniería Nuclear (CAB). Laboratorio Cavitación y Biotecnología; Argentina. Comisión Nacional de Energía Atómica. Gerencia del Área de Energía Nuclear. Instituto Balseiro; Argentina
Fil: Giana, Fabián Eduardo. Comisión Nacional de Energía Atómica. Gerencia de Área de Energía Nuclear. Gerencia de Ingeniería Nuclear (CAB). Laboratorio Cavitación y Biotecnología; Argentina. Comisión Nacional de Energía Atómica. Gerencia del Área de Energía Nuclear. Instituto Balseiro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina
Fil: Bonetto, Fabian Jose. Comisión Nacional de Energía Atómica. Gerencia de Área de Energía Nuclear. Gerencia de Ingeniería Nuclear (CAB). Laboratorio Cavitación y Biotecnología; Argentina. Comisión Nacional de Energía Atómica. Gerencia del Área de Energía Nuclear. Instituto Balseiro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina
description Electrical wound-healing assays are often used as a means to study in vitro cell migration and proliferation. In such analysis, a cell monolayer that sits on a small electrode is electrically wounded and its spectral impedance is then continuously measured in order to monitor the healing process. The relatively slow dynamics of the cell healing have been extensively studied, while those of the much faster wounding phase have not yet been investigated. An analysis of the electrical properties of a particular cell type during this phase could give extra information about the changes in the cell membrane due to the application of the wounding current, and could also be useful to optimize the wounding regime for different cell types. The main issue when trying to register information about these dynamics is that the traditional measurement scheme employed in typical wound-healing assays doesn't allow the simultaneous application of the wounding signal and measurement of the system's impedance. In this paper, we overcome this limitation by implementing a measurement strategy consisting of cycles of fast alternating low- and high-voltage signals applied on electrodes covered with mammalian cells. This approach is capable of registering the fast impedance changes during the transient regime corresponding to the cell wounding process. Furthermore, these quasi-simultaneous high- and low-voltage measurements can be compared in order to obtain an empirical correlation between both quantities.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-25
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/127057
Bellotti, Mariela; Giana, Fabián Eduardo; Bonetto, Fabian Jose; Impedance spectroscopy applied to the fast wounding dynamics of an electrical wound-healing assay in mammalian cells; IOP Publishing; Measurement Science & Technology (print); 26; 8; 25-6-2015; 1-11
0957-0233
1361-6501
CONICET Digital
CONICET
url http://hdl.handle.net/11336/127057
identifier_str_mv Bellotti, Mariela; Giana, Fabián Eduardo; Bonetto, Fabian Jose; Impedance spectroscopy applied to the fast wounding dynamics of an electrical wound-healing assay in mammalian cells; IOP Publishing; Measurement Science & Technology (print); 26; 8; 25-6-2015; 1-11
0957-0233
1361-6501
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1088/0957-0233/26/8/085701
info:eu-repo/semantics/altIdentifier/url/https://iopscience.iop.org/article/10.1088/0957-0233/26/8/085701
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv IOP Publishing
publisher.none.fl_str_mv IOP Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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