Simultaneous determination of aspartame and acesulfame-K by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromato...

Autores
Cantarelli, Miguel Angel; Pellerano, Roberto Gerardo; Marchevsky, Eduardo Jorge; Camiña, José Manuel
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A new method to determine mixtures of two sweeteners, aspartame and acesulfame-K, in commercial sweeteners is proposed. A classical 52 full factorial design for standards was used for calibration in the concentration matrix. Salicylic acid was used as internal standard in order to evaluate the adjustment of the real samples in the PLS-2 model. This model was obtained from UV spectral data, validated by internal cross-validation and was used to find the concentration of analytes in the commercial samples. The PLS-2 method was validated externally by high performance liquid chromatography (HPLC), finding, in all cases, a relative error of less than 10% between the PLS-2 and the HPLC methods. The mean value of recovery degree in real samples was 99.2% with standard deviation of 3.2%. The proposed procedure was applied successfully to the determination of mixtures of aspartame and acesulfame-K in artificial sweeteners.
Fil: Cantarelli, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Fil: Pellerano, Roberto Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Marchevsky, Eduardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Camiña, José Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Materia
Acesulfame-K
Aspartame
Hplc
Multivariate Calibration
Pls-2 Method
Sweeteners
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/81666
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Simultaneous determination of aspartame and acesulfame-K by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromatographyCantarelli, Miguel AngelPellerano, Roberto GerardoMarchevsky, Eduardo JorgeCamiña, José ManuelAcesulfame-KAspartameHplcMultivariate CalibrationPls-2 MethodSweetenershttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A new method to determine mixtures of two sweeteners, aspartame and acesulfame-K, in commercial sweeteners is proposed. A classical 52 full factorial design for standards was used for calibration in the concentration matrix. Salicylic acid was used as internal standard in order to evaluate the adjustment of the real samples in the PLS-2 model. This model was obtained from UV spectral data, validated by internal cross-validation and was used to find the concentration of analytes in the commercial samples. The PLS-2 method was validated externally by high performance liquid chromatography (HPLC), finding, in all cases, a relative error of less than 10% between the PLS-2 and the HPLC methods. The mean value of recovery degree in real samples was 99.2% with standard deviation of 3.2%. The proposed procedure was applied successfully to the determination of mixtures of aspartame and acesulfame-K in artificial sweeteners.Fil: Cantarelli, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; ArgentinaFil: Pellerano, Roberto Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Marchevsky, Eduardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Camiña, José Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; ArgentinaElsevier2009-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/81666Cantarelli, Miguel Angel; Pellerano, Roberto Gerardo; Marchevsky, Eduardo Jorge; Camiña, José Manuel; Simultaneous determination of aspartame and acesulfame-K by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromatography; Elsevier; Food Chemistry; 115; 3; 8-2009; 1128-11320308-8146CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0308814609000211info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2008.12.101info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:26Zoai:ri.conicet.gov.ar:11336/81666instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:27.204CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Simultaneous determination of aspartame and acesulfame-K by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromatography
title Simultaneous determination of aspartame and acesulfame-K by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromatography
spellingShingle Simultaneous determination of aspartame and acesulfame-K by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromatography
Cantarelli, Miguel Angel
Acesulfame-K
Aspartame
Hplc
Multivariate Calibration
Pls-2 Method
Sweeteners
title_short Simultaneous determination of aspartame and acesulfame-K by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromatography
title_full Simultaneous determination of aspartame and acesulfame-K by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromatography
title_fullStr Simultaneous determination of aspartame and acesulfame-K by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromatography
title_full_unstemmed Simultaneous determination of aspartame and acesulfame-K by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromatography
title_sort Simultaneous determination of aspartame and acesulfame-K by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromatography
dc.creator.none.fl_str_mv Cantarelli, Miguel Angel
Pellerano, Roberto Gerardo
Marchevsky, Eduardo Jorge
Camiña, José Manuel
author Cantarelli, Miguel Angel
author_facet Cantarelli, Miguel Angel
Pellerano, Roberto Gerardo
Marchevsky, Eduardo Jorge
Camiña, José Manuel
author_role author
author2 Pellerano, Roberto Gerardo
Marchevsky, Eduardo Jorge
Camiña, José Manuel
author2_role author
author
author
dc.subject.none.fl_str_mv Acesulfame-K
Aspartame
Hplc
Multivariate Calibration
Pls-2 Method
Sweeteners
topic Acesulfame-K
Aspartame
Hplc
Multivariate Calibration
Pls-2 Method
Sweeteners
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A new method to determine mixtures of two sweeteners, aspartame and acesulfame-K, in commercial sweeteners is proposed. A classical 52 full factorial design for standards was used for calibration in the concentration matrix. Salicylic acid was used as internal standard in order to evaluate the adjustment of the real samples in the PLS-2 model. This model was obtained from UV spectral data, validated by internal cross-validation and was used to find the concentration of analytes in the commercial samples. The PLS-2 method was validated externally by high performance liquid chromatography (HPLC), finding, in all cases, a relative error of less than 10% between the PLS-2 and the HPLC methods. The mean value of recovery degree in real samples was 99.2% with standard deviation of 3.2%. The proposed procedure was applied successfully to the determination of mixtures of aspartame and acesulfame-K in artificial sweeteners.
Fil: Cantarelli, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Fil: Pellerano, Roberto Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Marchevsky, Eduardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Camiña, José Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
description A new method to determine mixtures of two sweeteners, aspartame and acesulfame-K, in commercial sweeteners is proposed. A classical 52 full factorial design for standards was used for calibration in the concentration matrix. Salicylic acid was used as internal standard in order to evaluate the adjustment of the real samples in the PLS-2 model. This model was obtained from UV spectral data, validated by internal cross-validation and was used to find the concentration of analytes in the commercial samples. The PLS-2 method was validated externally by high performance liquid chromatography (HPLC), finding, in all cases, a relative error of less than 10% between the PLS-2 and the HPLC methods. The mean value of recovery degree in real samples was 99.2% with standard deviation of 3.2%. The proposed procedure was applied successfully to the determination of mixtures of aspartame and acesulfame-K in artificial sweeteners.
publishDate 2009
dc.date.none.fl_str_mv 2009-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/81666
Cantarelli, Miguel Angel; Pellerano, Roberto Gerardo; Marchevsky, Eduardo Jorge; Camiña, José Manuel; Simultaneous determination of aspartame and acesulfame-K by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromatography; Elsevier; Food Chemistry; 115; 3; 8-2009; 1128-1132
0308-8146
CONICET Digital
CONICET
url http://hdl.handle.net/11336/81666
identifier_str_mv Cantarelli, Miguel Angel; Pellerano, Roberto Gerardo; Marchevsky, Eduardo Jorge; Camiña, José Manuel; Simultaneous determination of aspartame and acesulfame-K by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromatography; Elsevier; Food Chemistry; 115; 3; 8-2009; 1128-1132
0308-8146
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0308814609000211
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2008.12.101
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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