Modified paramagnetic beads in a microfluidic system for the determination of ethinylestradiol (EE2) in river water samples

Autores
Martinez, Noelia Anabel; Schneider, Rudolf J.; Messina, Germán Alejandro; Raba, Julio
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work, we have developed and characterized a novel microfluidic immunoassay methodology for rapid and sensitive quantification of ethinylestradiol (EE2) in river water samples. The detection of EE2 was carried out using a competitive direct immunoassay method based on the use of anti-EE2 polyclonal antibodies immobilized on magnetic microspheres 3-aminopropyl-modified manipulated for an external removable magnet. The EE2 present in the water sample was allowed to compete with EE2-horseradish peroxidase (HPR) conjugated for the immobilized anti-EE2 antibody. The HPR, in the presence of hydrogen peroxide (H2O2) catalyzes the oxidation of catechol (Q) whose back electrochemical reduction was detected on gold electrode at 0.0 V. The response current obtained from the product of enzymatic reaction is inversely proportional to the amount of EE2 in the water sample. The electrochemical detection can be done within 1 min and total assay time was 30 min. The calculated detection limits for electrochemical detection and the ELISA procedure are 0.09 and 0.32 ng L-1 respectively and the intra- and inter-assay coefficients of variation were below 5.8%. Our electrochemical immunosensor showed higher sensitivity and lower time consumed than the standard spectrophotometric detection ELISA method, which shows the potential for assessment of EE2 in river water samples.
Fil: Martinez, Noelia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; Argentina
Fil: Rudolf J. Schneider. BAM-Federal Institute for Materials Research and Testing, Department of Analytical Chemistry; Alemania
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; Argentina
Materia
ENZYME IMMUNOASSAYS
ETHINYLESTRADIOL
FLOW INJECTION ANALYSIS
HORSERADISH PEROXIDASE
MICROFLUIDIC
PARAMAGNETIC BEADS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/92854
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/92854
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Modified paramagnetic beads in a microfluidic system for the determination of ethinylestradiol (EE2) in river water samplesMartinez, Noelia AnabelSchneider, Rudolf J.Messina, Germán AlejandroRaba, JulioENZYME IMMUNOASSAYSETHINYLESTRADIOLFLOW INJECTION ANALYSISHORSERADISH PEROXIDASEMICROFLUIDICPARAMAGNETIC BEADShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In this work, we have developed and characterized a novel microfluidic immunoassay methodology for rapid and sensitive quantification of ethinylestradiol (EE2) in river water samples. The detection of EE2 was carried out using a competitive direct immunoassay method based on the use of anti-EE2 polyclonal antibodies immobilized on magnetic microspheres 3-aminopropyl-modified manipulated for an external removable magnet. The EE2 present in the water sample was allowed to compete with EE2-horseradish peroxidase (HPR) conjugated for the immobilized anti-EE2 antibody. The HPR, in the presence of hydrogen peroxide (H2O2) catalyzes the oxidation of catechol (Q) whose back electrochemical reduction was detected on gold electrode at 0.0 V. The response current obtained from the product of enzymatic reaction is inversely proportional to the amount of EE2 in the water sample. The electrochemical detection can be done within 1 min and total assay time was 30 min. The calculated detection limits for electrochemical detection and the ELISA procedure are 0.09 and 0.32 ng L-1 respectively and the intra- and inter-assay coefficients of variation were below 5.8%. Our electrochemical immunosensor showed higher sensitivity and lower time consumed than the standard spectrophotometric detection ELISA method, which shows the potential for assessment of EE2 in river water samples.Fil: Martinez, Noelia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; ArgentinaFil: Rudolf J. Schneider. BAM-Federal Institute for Materials Research and Testing, Department of Analytical Chemistry; AlemaniaFil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; ArgentinaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; ArgentinaElsevier Advanced Technology2010-03-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92854Martinez, Noelia Anabel; Schneider, Rudolf J.; Messina, Germán Alejandro; Raba, Julio; Modified paramagnetic beads in a microfluidic system for the determination of ethinylestradiol (EE2) in river water samples; Elsevier Advanced Technology; Biosensors & Bioelectronics; 25; 6; 30-2-2010; 1376-13810956-5663CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bios.2009.10.031info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0956566309005909info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:47:23Zoai:ri.conicet.gov.ar:11336/92854instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:47:23.611CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Modified paramagnetic beads in a microfluidic system for the determination of ethinylestradiol (EE2) in river water samples
title Modified paramagnetic beads in a microfluidic system for the determination of ethinylestradiol (EE2) in river water samples
spellingShingle Modified paramagnetic beads in a microfluidic system for the determination of ethinylestradiol (EE2) in river water samples
Martinez, Noelia Anabel
ENZYME IMMUNOASSAYS
ETHINYLESTRADIOL
FLOW INJECTION ANALYSIS
HORSERADISH PEROXIDASE
MICROFLUIDIC
PARAMAGNETIC BEADS
title_short Modified paramagnetic beads in a microfluidic system for the determination of ethinylestradiol (EE2) in river water samples
title_full Modified paramagnetic beads in a microfluidic system for the determination of ethinylestradiol (EE2) in river water samples
title_fullStr Modified paramagnetic beads in a microfluidic system for the determination of ethinylestradiol (EE2) in river water samples
title_full_unstemmed Modified paramagnetic beads in a microfluidic system for the determination of ethinylestradiol (EE2) in river water samples
title_sort Modified paramagnetic beads in a microfluidic system for the determination of ethinylestradiol (EE2) in river water samples
dc.creator.none.fl_str_mv Martinez, Noelia Anabel
Schneider, Rudolf J.
Messina, Germán Alejandro
Raba, Julio
author Martinez, Noelia Anabel
author_facet Martinez, Noelia Anabel
Schneider, Rudolf J.
Messina, Germán Alejandro
Raba, Julio
author_role author
author2 Schneider, Rudolf J.
Messina, Germán Alejandro
Raba, Julio
author2_role author
author
author
dc.subject.none.fl_str_mv ENZYME IMMUNOASSAYS
ETHINYLESTRADIOL
FLOW INJECTION ANALYSIS
HORSERADISH PEROXIDASE
MICROFLUIDIC
PARAMAGNETIC BEADS
topic ENZYME IMMUNOASSAYS
ETHINYLESTRADIOL
FLOW INJECTION ANALYSIS
HORSERADISH PEROXIDASE
MICROFLUIDIC
PARAMAGNETIC BEADS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this work, we have developed and characterized a novel microfluidic immunoassay methodology for rapid and sensitive quantification of ethinylestradiol (EE2) in river water samples. The detection of EE2 was carried out using a competitive direct immunoassay method based on the use of anti-EE2 polyclonal antibodies immobilized on magnetic microspheres 3-aminopropyl-modified manipulated for an external removable magnet. The EE2 present in the water sample was allowed to compete with EE2-horseradish peroxidase (HPR) conjugated for the immobilized anti-EE2 antibody. The HPR, in the presence of hydrogen peroxide (H2O2) catalyzes the oxidation of catechol (Q) whose back electrochemical reduction was detected on gold electrode at 0.0 V. The response current obtained from the product of enzymatic reaction is inversely proportional to the amount of EE2 in the water sample. The electrochemical detection can be done within 1 min and total assay time was 30 min. The calculated detection limits for electrochemical detection and the ELISA procedure are 0.09 and 0.32 ng L-1 respectively and the intra- and inter-assay coefficients of variation were below 5.8%. Our electrochemical immunosensor showed higher sensitivity and lower time consumed than the standard spectrophotometric detection ELISA method, which shows the potential for assessment of EE2 in river water samples.
Fil: Martinez, Noelia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; Argentina
Fil: Rudolf J. Schneider. BAM-Federal Institute for Materials Research and Testing, Department of Analytical Chemistry; Alemania
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; Argentina
description In this work, we have developed and characterized a novel microfluidic immunoassay methodology for rapid and sensitive quantification of ethinylestradiol (EE2) in river water samples. The detection of EE2 was carried out using a competitive direct immunoassay method based on the use of anti-EE2 polyclonal antibodies immobilized on magnetic microspheres 3-aminopropyl-modified manipulated for an external removable magnet. The EE2 present in the water sample was allowed to compete with EE2-horseradish peroxidase (HPR) conjugated for the immobilized anti-EE2 antibody. The HPR, in the presence of hydrogen peroxide (H2O2) catalyzes the oxidation of catechol (Q) whose back electrochemical reduction was detected on gold electrode at 0.0 V. The response current obtained from the product of enzymatic reaction is inversely proportional to the amount of EE2 in the water sample. The electrochemical detection can be done within 1 min and total assay time was 30 min. The calculated detection limits for electrochemical detection and the ELISA procedure are 0.09 and 0.32 ng L-1 respectively and the intra- and inter-assay coefficients of variation were below 5.8%. Our electrochemical immunosensor showed higher sensitivity and lower time consumed than the standard spectrophotometric detection ELISA method, which shows the potential for assessment of EE2 in river water samples.
publishDate 2010
dc.date.none.fl_str_mv 2010-03-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/92854
Martinez, Noelia Anabel; Schneider, Rudolf J.; Messina, Germán Alejandro; Raba, Julio; Modified paramagnetic beads in a microfluidic system for the determination of ethinylestradiol (EE2) in river water samples; Elsevier Advanced Technology; Biosensors & Bioelectronics; 25; 6; 30-2-2010; 1376-1381
0956-5663
CONICET Digital
CONICET
url http://hdl.handle.net/11336/92854
identifier_str_mv Martinez, Noelia Anabel; Schneider, Rudolf J.; Messina, Germán Alejandro; Raba, Julio; Modified paramagnetic beads in a microfluidic system for the determination of ethinylestradiol (EE2) in river water samples; Elsevier Advanced Technology; Biosensors & Bioelectronics; 25; 6; 30-2-2010; 1376-1381
0956-5663
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bios.2009.10.031
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0956566309005909
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Advanced Technology
publisher.none.fl_str_mv Elsevier Advanced Technology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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