Microfluidic immunosensor with micromagnetic beads coupled to carbon-based screen-printed electrodes (SPCEs) for determination of botrytis cinerea in tissue of fruits

Autores
Fernández Baldo, Martín Alejandro; Messina, Germán Alejandro; Sanz Ferramola, Maria Isabel; Raba, Julio
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A wide range of plant species, including economically important crops such as vegetables, ornamentals, bulbs, and fundamentally fruits, can be affected by gray mold caused by the fungal pathogen Botrytis cinerea. This paper describes the development of a microfluidic immunosensor with micromagnetic beads (MMBs) coupled to carbon-based screen-printed electrodes (SPCEs) for the rapid and sensitive quantification of B. cinerea in apple (Red Delicious), table grape (pink Moscatel), and pear (William's) tissues. The detection of B. cinerea was carried out using a competitive immunoassay method based on the use of purified B. cinerea antigens immobilized on 3-aminopropyl-modified MMBs. The total assay time was 40 min, and the calculated detection limit was 0.008 μg mL -1. Moreover, the intra-and interassay coefficients of variation were below 7%. The developed method allowed detects B. cinerea even in asymptomatic fruits and promises to be particularly useful for application in the agricultural industry.
Fil: Fernández Baldo, Martín Alejandro. Microfluidic Immunosensor with Micromagnetic Beads Coupled to Carbon-Based Screen-Printed Electrodes (SPCEs) for Determination of Botrytis cinerea in Tissue of Fruits; Argentina
Fil: Messina, Germán Alejandro. Microfluidic Immunosensor with Micromagnetic Beads Coupled to Carbon-Based Screen-Printed Electrodes (SPCEs) for Determination of Botrytis cinerea in Tissue of Fruits; Argentina
Fil: Sanz Ferramola, Maria Isabel. Microfluidic Immunosensor with Micromagnetic Beads Coupled to Carbon-Based Screen-Printed Electrodes (SPCEs) for Determination of Botrytis cinerea in Tissue of Fruits; Argentina
Fil: Raba, Julio. Microfluidic Immunosensor with Micromagnetic Beads Coupled to Carbon-Based Screen-Printed Electrodes (SPCEs) for Determination of Botrytis cinerea in Tissue of Fruits; Argentina
Materia
BOTRYTIS CINEREA
ENZYME IMMUNOASSAYS
FLOW INJECTION ANALYSIS
HORSERADISH PEROXIDASE
MICROFLUIDIC SYSTEM
MICROMAGNETIC BEADS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/92876
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/92876
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Microfluidic immunosensor with micromagnetic beads coupled to carbon-based screen-printed electrodes (SPCEs) for determination of botrytis cinerea in tissue of fruitsFernández Baldo, Martín AlejandroMessina, Germán AlejandroSanz Ferramola, Maria IsabelRaba, JulioBOTRYTIS CINEREAENZYME IMMUNOASSAYSFLOW INJECTION ANALYSISHORSERADISH PEROXIDASEMICROFLUIDIC SYSTEMMICROMAGNETIC BEADShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A wide range of plant species, including economically important crops such as vegetables, ornamentals, bulbs, and fundamentally fruits, can be affected by gray mold caused by the fungal pathogen Botrytis cinerea. This paper describes the development of a microfluidic immunosensor with micromagnetic beads (MMBs) coupled to carbon-based screen-printed electrodes (SPCEs) for the rapid and sensitive quantification of B. cinerea in apple (Red Delicious), table grape (pink Moscatel), and pear (William's) tissues. The detection of B. cinerea was carried out using a competitive immunoassay method based on the use of purified B. cinerea antigens immobilized on 3-aminopropyl-modified MMBs. The total assay time was 40 min, and the calculated detection limit was 0.008 μg mL -1. Moreover, the intra-and interassay coefficients of variation were below 7%. The developed method allowed detects B. cinerea even in asymptomatic fruits and promises to be particularly useful for application in the agricultural industry.Fil: Fernández Baldo, Martín Alejandro. Microfluidic Immunosensor with Micromagnetic Beads Coupled to Carbon-Based Screen-Printed Electrodes (SPCEs) for Determination of Botrytis cinerea in Tissue of Fruits; ArgentinaFil: Messina, Germán Alejandro. Microfluidic Immunosensor with Micromagnetic Beads Coupled to Carbon-Based Screen-Printed Electrodes (SPCEs) for Determination of Botrytis cinerea in Tissue of Fruits; ArgentinaFil: Sanz Ferramola, Maria Isabel. Microfluidic Immunosensor with Micromagnetic Beads Coupled to Carbon-Based Screen-Printed Electrodes (SPCEs) for Determination of Botrytis cinerea in Tissue of Fruits; ArgentinaFil: Raba, Julio. Microfluidic Immunosensor with Micromagnetic Beads Coupled to Carbon-Based Screen-Printed Electrodes (SPCEs) for Determination of Botrytis cinerea in Tissue of Fruits; ArgentinaAmerican Chemical Society2010-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92876Fernández Baldo, Martín Alejandro; Messina, Germán Alejandro; Sanz Ferramola, Maria Isabel; Raba, Julio; Microfluidic immunosensor with micromagnetic beads coupled to carbon-based screen-printed electrodes (SPCEs) for determination of botrytis cinerea in tissue of fruits; American Chemical Society; Journal of Agricultural and Food Chemistry; 58; 21; 11-2010; 11201-112060021-8561CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1021/jf1025604info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/jf1025604info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:00:39Zoai:ri.conicet.gov.ar:11336/92876instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:00:39.631CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Microfluidic immunosensor with micromagnetic beads coupled to carbon-based screen-printed electrodes (SPCEs) for determination of botrytis cinerea in tissue of fruits
title Microfluidic immunosensor with micromagnetic beads coupled to carbon-based screen-printed electrodes (SPCEs) for determination of botrytis cinerea in tissue of fruits
spellingShingle Microfluidic immunosensor with micromagnetic beads coupled to carbon-based screen-printed electrodes (SPCEs) for determination of botrytis cinerea in tissue of fruits
Fernández Baldo, Martín Alejandro
BOTRYTIS CINEREA
ENZYME IMMUNOASSAYS
FLOW INJECTION ANALYSIS
HORSERADISH PEROXIDASE
MICROFLUIDIC SYSTEM
MICROMAGNETIC BEADS
title_short Microfluidic immunosensor with micromagnetic beads coupled to carbon-based screen-printed electrodes (SPCEs) for determination of botrytis cinerea in tissue of fruits
title_full Microfluidic immunosensor with micromagnetic beads coupled to carbon-based screen-printed electrodes (SPCEs) for determination of botrytis cinerea in tissue of fruits
title_fullStr Microfluidic immunosensor with micromagnetic beads coupled to carbon-based screen-printed electrodes (SPCEs) for determination of botrytis cinerea in tissue of fruits
title_full_unstemmed Microfluidic immunosensor with micromagnetic beads coupled to carbon-based screen-printed electrodes (SPCEs) for determination of botrytis cinerea in tissue of fruits
title_sort Microfluidic immunosensor with micromagnetic beads coupled to carbon-based screen-printed electrodes (SPCEs) for determination of botrytis cinerea in tissue of fruits
dc.creator.none.fl_str_mv Fernández Baldo, Martín Alejandro
Messina, Germán Alejandro
Sanz Ferramola, Maria Isabel
Raba, Julio
author Fernández Baldo, Martín Alejandro
author_facet Fernández Baldo, Martín Alejandro
Messina, Germán Alejandro
Sanz Ferramola, Maria Isabel
Raba, Julio
author_role author
author2 Messina, Germán Alejandro
Sanz Ferramola, Maria Isabel
Raba, Julio
author2_role author
author
author
dc.subject.none.fl_str_mv BOTRYTIS CINEREA
ENZYME IMMUNOASSAYS
FLOW INJECTION ANALYSIS
HORSERADISH PEROXIDASE
MICROFLUIDIC SYSTEM
MICROMAGNETIC BEADS
topic BOTRYTIS CINEREA
ENZYME IMMUNOASSAYS
FLOW INJECTION ANALYSIS
HORSERADISH PEROXIDASE
MICROFLUIDIC SYSTEM
MICROMAGNETIC BEADS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A wide range of plant species, including economically important crops such as vegetables, ornamentals, bulbs, and fundamentally fruits, can be affected by gray mold caused by the fungal pathogen Botrytis cinerea. This paper describes the development of a microfluidic immunosensor with micromagnetic beads (MMBs) coupled to carbon-based screen-printed electrodes (SPCEs) for the rapid and sensitive quantification of B. cinerea in apple (Red Delicious), table grape (pink Moscatel), and pear (William's) tissues. The detection of B. cinerea was carried out using a competitive immunoassay method based on the use of purified B. cinerea antigens immobilized on 3-aminopropyl-modified MMBs. The total assay time was 40 min, and the calculated detection limit was 0.008 μg mL -1. Moreover, the intra-and interassay coefficients of variation were below 7%. The developed method allowed detects B. cinerea even in asymptomatic fruits and promises to be particularly useful for application in the agricultural industry.
Fil: Fernández Baldo, Martín Alejandro. Microfluidic Immunosensor with Micromagnetic Beads Coupled to Carbon-Based Screen-Printed Electrodes (SPCEs) for Determination of Botrytis cinerea in Tissue of Fruits; Argentina
Fil: Messina, Germán Alejandro. Microfluidic Immunosensor with Micromagnetic Beads Coupled to Carbon-Based Screen-Printed Electrodes (SPCEs) for Determination of Botrytis cinerea in Tissue of Fruits; Argentina
Fil: Sanz Ferramola, Maria Isabel. Microfluidic Immunosensor with Micromagnetic Beads Coupled to Carbon-Based Screen-Printed Electrodes (SPCEs) for Determination of Botrytis cinerea in Tissue of Fruits; Argentina
Fil: Raba, Julio. Microfluidic Immunosensor with Micromagnetic Beads Coupled to Carbon-Based Screen-Printed Electrodes (SPCEs) for Determination of Botrytis cinerea in Tissue of Fruits; Argentina
description A wide range of plant species, including economically important crops such as vegetables, ornamentals, bulbs, and fundamentally fruits, can be affected by gray mold caused by the fungal pathogen Botrytis cinerea. This paper describes the development of a microfluidic immunosensor with micromagnetic beads (MMBs) coupled to carbon-based screen-printed electrodes (SPCEs) for the rapid and sensitive quantification of B. cinerea in apple (Red Delicious), table grape (pink Moscatel), and pear (William's) tissues. The detection of B. cinerea was carried out using a competitive immunoassay method based on the use of purified B. cinerea antigens immobilized on 3-aminopropyl-modified MMBs. The total assay time was 40 min, and the calculated detection limit was 0.008 μg mL -1. Moreover, the intra-and interassay coefficients of variation were below 7%. The developed method allowed detects B. cinerea even in asymptomatic fruits and promises to be particularly useful for application in the agricultural industry.
publishDate 2010
dc.date.none.fl_str_mv 2010-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/92876
Fernández Baldo, Martín Alejandro; Messina, Germán Alejandro; Sanz Ferramola, Maria Isabel; Raba, Julio; Microfluidic immunosensor with micromagnetic beads coupled to carbon-based screen-printed electrodes (SPCEs) for determination of botrytis cinerea in tissue of fruits; American Chemical Society; Journal of Agricultural and Food Chemistry; 58; 21; 11-2010; 11201-11206
0021-8561
CONICET Digital
CONICET
url http://hdl.handle.net/11336/92876
identifier_str_mv Fernández Baldo, Martín Alejandro; Messina, Germán Alejandro; Sanz Ferramola, Maria Isabel; Raba, Julio; Microfluidic immunosensor with micromagnetic beads coupled to carbon-based screen-printed electrodes (SPCEs) for determination of botrytis cinerea in tissue of fruits; American Chemical Society; Journal of Agricultural and Food Chemistry; 58; 21; 11-2010; 11201-11206
0021-8561
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1021/jf1025604
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/jf1025604
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613790140727296
score 13.070432

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