Modified paramagnetic beads in a microfluidic system for the determination of zearalenone in feedstuffs samples

Autores
Panini, Nancy Veronica; Salinas, Eloy; Messina, Germán Alejandro; Raba, Julio
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work, we have developed and characterised a novel microfluidic immunoassay methodology for rapid and sensitive quantification of ZEA in feedstuffs samples. The detection of ZEA was carried out using a competitive direct immunoassay method based on the use of anti-ZEA monoclonal antibodies immobilized on magnetic microspheres 3-aminopropyl-modified manipulated for an external remobilize magnets. The ZEA in feedstuffs sample is allowed to compete with ZEA-horseradish peroxidase (HPR) conjugated for the immobilized anti-ZEA antibody. The HPR, in the presence of hydrogen peroxide (H2O2) catalyses the oxidation of 4-tert-butylcatechol (4-TBC) whose back electrochemical reduction was detected on gold electrode at 0.0V. The calculated detection limits for electrochemical detection and ELISA procedure were 0.41 and 2.56μgkg-1 respectively, the intra and inter-assay coefficients of variation were below 6.5% and the total assay time was 30min. The microfluidic immunosensor showed higher sensitivity and lower detection limits than the standard ELISA method, which shows potential for detecting ZEA in foods and feeds diagnosis.
Fil: Panini, Nancy Veronica. Universidad Nacional de San Luis; Argentina
Fil: Salinas Eloy. Universidad Nacional de San Luis; Argentina
Fil: Messina, Germán Alejandro. Instituto de Química de San Luis, Universidad Nacional de San luis, Fcaultad de Quimica, Bioquimica y Farmacia.; Argentina
Fil: Raba, Julio. Instituto de Química de San Luis, Universidad Nacional de San luis, Fcaultad de Quimica, Bioquimica y Farmacia.; Argentina; Argentina
Materia
ENZYME IMMUNOASSAYS
FLOW INJECTION ANALYSIS
HORSERADISH PEROXIDASE
MICROFLUIDIC
PARAMAGNETIC BEADS
ZEARALENONE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/92856
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/92856
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Modified paramagnetic beads in a microfluidic system for the determination of zearalenone in feedstuffs samplesPanini, Nancy VeronicaSalinas, EloyMessina, Germán AlejandroRaba, JulioENZYME IMMUNOASSAYSFLOW INJECTION ANALYSISHORSERADISH PEROXIDASEMICROFLUIDICPARAMAGNETIC BEADSZEARALENONEhttps://purl.org/becyt/ford/4.2https://purl.org/becyt/ford/4In this work, we have developed and characterised a novel microfluidic immunoassay methodology for rapid and sensitive quantification of ZEA in feedstuffs samples. The detection of ZEA was carried out using a competitive direct immunoassay method based on the use of anti-ZEA monoclonal antibodies immobilized on magnetic microspheres 3-aminopropyl-modified manipulated for an external remobilize magnets. The ZEA in feedstuffs sample is allowed to compete with ZEA-horseradish peroxidase (HPR) conjugated for the immobilized anti-ZEA antibody. The HPR, in the presence of hydrogen peroxide (H2O2) catalyses the oxidation of 4-tert-butylcatechol (4-TBC) whose back electrochemical reduction was detected on gold electrode at 0.0V. The calculated detection limits for electrochemical detection and ELISA procedure were 0.41 and 2.56μgkg-1 respectively, the intra and inter-assay coefficients of variation were below 6.5% and the total assay time was 30min. The microfluidic immunosensor showed higher sensitivity and lower detection limits than the standard ELISA method, which shows potential for detecting ZEA in foods and feeds diagnosis.Fil: Panini, Nancy Veronica. Universidad Nacional de San Luis; ArgentinaFil: Salinas Eloy. Universidad Nacional de San Luis; ArgentinaFil: Messina, Germán Alejandro. Instituto de Química de San Luis, Universidad Nacional de San luis, Fcaultad de Quimica, Bioquimica y Farmacia.; ArgentinaFil: Raba, Julio. Instituto de Química de San Luis, Universidad Nacional de San luis, Fcaultad de Quimica, Bioquimica y Farmacia.; Argentina; ArgentinaElsevier2011-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92856Panini, Nancy Veronica; Salinas, Eloy; Messina, Germán Alejandro; Raba, Julio; Modified paramagnetic beads in a microfluidic system for the determination of zearalenone in feedstuffs samples; Elsevier; Food Chemistry; 125; 2; 3-2011; 791-7960308-8146CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2010.09.035info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0308814610011088info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:58:33Zoai:ri.conicet.gov.ar:11336/92856instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:58:33.46CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Modified paramagnetic beads in a microfluidic system for the determination of zearalenone in feedstuffs samples
title Modified paramagnetic beads in a microfluidic system for the determination of zearalenone in feedstuffs samples
spellingShingle Modified paramagnetic beads in a microfluidic system for the determination of zearalenone in feedstuffs samples
Panini, Nancy Veronica
ENZYME IMMUNOASSAYS
FLOW INJECTION ANALYSIS
HORSERADISH PEROXIDASE
MICROFLUIDIC
PARAMAGNETIC BEADS
ZEARALENONE
title_short Modified paramagnetic beads in a microfluidic system for the determination of zearalenone in feedstuffs samples
title_full Modified paramagnetic beads in a microfluidic system for the determination of zearalenone in feedstuffs samples
title_fullStr Modified paramagnetic beads in a microfluidic system for the determination of zearalenone in feedstuffs samples
title_full_unstemmed Modified paramagnetic beads in a microfluidic system for the determination of zearalenone in feedstuffs samples
title_sort Modified paramagnetic beads in a microfluidic system for the determination of zearalenone in feedstuffs samples
dc.creator.none.fl_str_mv Panini, Nancy Veronica
Salinas, Eloy
Messina, Germán Alejandro
Raba, Julio
author Panini, Nancy Veronica
author_facet Panini, Nancy Veronica
Salinas, Eloy
Messina, Germán Alejandro
Raba, Julio
author_role author
author2 Salinas, Eloy
Messina, Germán Alejandro
Raba, Julio
author2_role author
author
author
dc.subject.none.fl_str_mv ENZYME IMMUNOASSAYS
FLOW INJECTION ANALYSIS
HORSERADISH PEROXIDASE
MICROFLUIDIC
PARAMAGNETIC BEADS
ZEARALENONE
topic ENZYME IMMUNOASSAYS
FLOW INJECTION ANALYSIS
HORSERADISH PEROXIDASE
MICROFLUIDIC
PARAMAGNETIC BEADS
ZEARALENONE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.2
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv In this work, we have developed and characterised a novel microfluidic immunoassay methodology for rapid and sensitive quantification of ZEA in feedstuffs samples. The detection of ZEA was carried out using a competitive direct immunoassay method based on the use of anti-ZEA monoclonal antibodies immobilized on magnetic microspheres 3-aminopropyl-modified manipulated for an external remobilize magnets. The ZEA in feedstuffs sample is allowed to compete with ZEA-horseradish peroxidase (HPR) conjugated for the immobilized anti-ZEA antibody. The HPR, in the presence of hydrogen peroxide (H2O2) catalyses the oxidation of 4-tert-butylcatechol (4-TBC) whose back electrochemical reduction was detected on gold electrode at 0.0V. The calculated detection limits for electrochemical detection and ELISA procedure were 0.41 and 2.56μgkg-1 respectively, the intra and inter-assay coefficients of variation were below 6.5% and the total assay time was 30min. The microfluidic immunosensor showed higher sensitivity and lower detection limits than the standard ELISA method, which shows potential for detecting ZEA in foods and feeds diagnosis.
Fil: Panini, Nancy Veronica. Universidad Nacional de San Luis; Argentina
Fil: Salinas Eloy. Universidad Nacional de San Luis; Argentina
Fil: Messina, Germán Alejandro. Instituto de Química de San Luis, Universidad Nacional de San luis, Fcaultad de Quimica, Bioquimica y Farmacia.; Argentina
Fil: Raba, Julio. Instituto de Química de San Luis, Universidad Nacional de San luis, Fcaultad de Quimica, Bioquimica y Farmacia.; Argentina; Argentina
description In this work, we have developed and characterised a novel microfluidic immunoassay methodology for rapid and sensitive quantification of ZEA in feedstuffs samples. The detection of ZEA was carried out using a competitive direct immunoassay method based on the use of anti-ZEA monoclonal antibodies immobilized on magnetic microspheres 3-aminopropyl-modified manipulated for an external remobilize magnets. The ZEA in feedstuffs sample is allowed to compete with ZEA-horseradish peroxidase (HPR) conjugated for the immobilized anti-ZEA antibody. The HPR, in the presence of hydrogen peroxide (H2O2) catalyses the oxidation of 4-tert-butylcatechol (4-TBC) whose back electrochemical reduction was detected on gold electrode at 0.0V. The calculated detection limits for electrochemical detection and ELISA procedure were 0.41 and 2.56μgkg-1 respectively, the intra and inter-assay coefficients of variation were below 6.5% and the total assay time was 30min. The microfluidic immunosensor showed higher sensitivity and lower detection limits than the standard ELISA method, which shows potential for detecting ZEA in foods and feeds diagnosis.
publishDate 2011
dc.date.none.fl_str_mv 2011-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/92856
Panini, Nancy Veronica; Salinas, Eloy; Messina, Germán Alejandro; Raba, Julio; Modified paramagnetic beads in a microfluidic system for the determination of zearalenone in feedstuffs samples; Elsevier; Food Chemistry; 125; 2; 3-2011; 791-796
0308-8146
CONICET Digital
CONICET
url http://hdl.handle.net/11336/92856
identifier_str_mv Panini, Nancy Veronica; Salinas, Eloy; Messina, Germán Alejandro; Raba, Julio; Modified paramagnetic beads in a microfluidic system for the determination of zearalenone in feedstuffs samples; Elsevier; Food Chemistry; 125; 2; 3-2011; 791-796
0308-8146
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2010.09.035
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0308814610011088
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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