Nassella trichotoma (Poales:Poaceae) crown and root rot diseases in Argentina revisited: preliminary results

Autores
Angeletti, Bárbara; Daddario, Juan Facundo Fabian; Loydi, Alejandro; Anderson, Freda Elizabeth
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Nassella trichotoma, a grass species native to South America, has become a noxious weed in many regions of the world. In Australia and New Zealand, it has been targeted for classical biological control. Initial studies to identify potential fungal biocontrol agents started in 1999 in Argentina and ended three years later having discarded a rust and a smut fungus. Work on a crown rot associated with tussock die-back symptoms, and believed to be caused by a corticioid fungus at the time, was less detailed, and abandoned with inconclusive results. Almost 20 years later the problem is being revisited, with research focused on plants with die-back symptoms. New surveys have been conducted and diseased plants collected and studied in the laboratory. Surprisingly, five different corticioid fungi were found associated with the crowns and roots of declining tussocks. Several species of Fusarium, belonging to four different species complexes, were also isolated from crowns and/or roots of declining tussocks, while Bipolaris sp. was isolated from a seed of N. trichotoma recovered from a burial trial within a patch of diseased plants. Artificial inoculations with all the obtained isolates were carried out on healthy N. trichotoma plantlets. After many failed inoculation experiments, it was concluded that the corticioid fungi were not acting as pathogens but rather as secondary colonisers on senescing plant material. In contrast, all the plantlets inoculated with Bipolaris sp. and Fusarium spp. developed some degree of crown and root discoloration and rot, and the inoculated fungi were all re-isolated from affected tissues. Because these pathogens were also found associated with seeds recovered from the soil around diseased plants, healthy seeds were inoculated with Bipolaris sp. and one Fusarium isolate in a separate experiment, causing a great decrease in their germination compared with un-inoculated ones. Surviving seedlings were less vigorous than those grown from healthy seeds. Overall, although all the tested isolates (other than the corticioid ones) were shown to cause some level of disease, none of them produced the die-back symptoms observed in the field. It is now presumed those are caused by more than one of these pathogens acting together in an additive or synergic manner. Moreover, at least two of them have been shown to have an impact on plant recruitment processes. More work is needed to clarify the etiology of this disease in Argentina and to fully assess the suitability of the involved fungi as biocontrol agents.
Fil: Angeletti, Bárbara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Daddario, Juan Facundo Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Loydi, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Anderson, Freda Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
XVI International Symposium on Biological Control of Weeds
Iguazú
Argentina
Fundación para el estudio de especies invasivas
Centro de Recursos Naturales Renovables de la Zona Semiárida
Materia
Nassella trichotoma
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/222689
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Nassella trichotoma (Poales:Poaceae) crown and root rot diseases in Argentina revisited: preliminary resultsAngeletti, BárbaraDaddario, Juan Facundo FabianLoydi, AlejandroAnderson, Freda ElizabethNassella trichotomahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Nassella trichotoma, a grass species native to South America, has become a noxious weed in many regions of the world. In Australia and New Zealand, it has been targeted for classical biological control. Initial studies to identify potential fungal biocontrol agents started in 1999 in Argentina and ended three years later having discarded a rust and a smut fungus. Work on a crown rot associated with tussock die-back symptoms, and believed to be caused by a corticioid fungus at the time, was less detailed, and abandoned with inconclusive results. Almost 20 years later the problem is being revisited, with research focused on plants with die-back symptoms. New surveys have been conducted and diseased plants collected and studied in the laboratory. Surprisingly, five different corticioid fungi were found associated with the crowns and roots of declining tussocks. Several species of Fusarium, belonging to four different species complexes, were also isolated from crowns and/or roots of declining tussocks, while Bipolaris sp. was isolated from a seed of N. trichotoma recovered from a burial trial within a patch of diseased plants. Artificial inoculations with all the obtained isolates were carried out on healthy N. trichotoma plantlets. After many failed inoculation experiments, it was concluded that the corticioid fungi were not acting as pathogens but rather as secondary colonisers on senescing plant material. In contrast, all the plantlets inoculated with Bipolaris sp. and Fusarium spp. developed some degree of crown and root discoloration and rot, and the inoculated fungi were all re-isolated from affected tissues. Because these pathogens were also found associated with seeds recovered from the soil around diseased plants, healthy seeds were inoculated with Bipolaris sp. and one Fusarium isolate in a separate experiment, causing a great decrease in their germination compared with un-inoculated ones. Surviving seedlings were less vigorous than those grown from healthy seeds. Overall, although all the tested isolates (other than the corticioid ones) were shown to cause some level of disease, none of them produced the die-back symptoms observed in the field. It is now presumed those are caused by more than one of these pathogens acting together in an additive or synergic manner. Moreover, at least two of them have been shown to have an impact on plant recruitment processes. More work is needed to clarify the etiology of this disease in Argentina and to fully assess the suitability of the involved fungi as biocontrol agents.Fil: Angeletti, Bárbara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Daddario, Juan Facundo Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Loydi, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Anderson, Freda Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaXVI International Symposium on Biological Control of WeedsIguazúArgentinaFundación para el estudio de especies invasivasCentro de Recursos Naturales Renovables de la Zona SemiáridaInternational Symposium on biological control of weeds2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectSimposioBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/222689Nassella trichotoma (Poales:Poaceae) crown and root rot diseases in Argentina revisited: preliminary results; XVI International Symposium on Biological Control of Weeds; Iguazú; Argentina; 2023; 48-48978-631-90256-0-6CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.iobc-global.org/global_sg_Classical_Weed_BC.htmlInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:29:14Zoai:ri.conicet.gov.ar:11336/222689instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:29:14.936CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nassella trichotoma (Poales:Poaceae) crown and root rot diseases in Argentina revisited: preliminary results
title Nassella trichotoma (Poales:Poaceae) crown and root rot diseases in Argentina revisited: preliminary results
spellingShingle Nassella trichotoma (Poales:Poaceae) crown and root rot diseases in Argentina revisited: preliminary results
Angeletti, Bárbara
Nassella trichotoma
title_short Nassella trichotoma (Poales:Poaceae) crown and root rot diseases in Argentina revisited: preliminary results
title_full Nassella trichotoma (Poales:Poaceae) crown and root rot diseases in Argentina revisited: preliminary results
title_fullStr Nassella trichotoma (Poales:Poaceae) crown and root rot diseases in Argentina revisited: preliminary results
title_full_unstemmed Nassella trichotoma (Poales:Poaceae) crown and root rot diseases in Argentina revisited: preliminary results
title_sort Nassella trichotoma (Poales:Poaceae) crown and root rot diseases in Argentina revisited: preliminary results
dc.creator.none.fl_str_mv Angeletti, Bárbara
Daddario, Juan Facundo Fabian
Loydi, Alejandro
Anderson, Freda Elizabeth
author Angeletti, Bárbara
author_facet Angeletti, Bárbara
Daddario, Juan Facundo Fabian
Loydi, Alejandro
Anderson, Freda Elizabeth
author_role author
author2 Daddario, Juan Facundo Fabian
Loydi, Alejandro
Anderson, Freda Elizabeth
author2_role author
author
author
dc.subject.none.fl_str_mv Nassella trichotoma
topic Nassella trichotoma
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Nassella trichotoma, a grass species native to South America, has become a noxious weed in many regions of the world. In Australia and New Zealand, it has been targeted for classical biological control. Initial studies to identify potential fungal biocontrol agents started in 1999 in Argentina and ended three years later having discarded a rust and a smut fungus. Work on a crown rot associated with tussock die-back symptoms, and believed to be caused by a corticioid fungus at the time, was less detailed, and abandoned with inconclusive results. Almost 20 years later the problem is being revisited, with research focused on plants with die-back symptoms. New surveys have been conducted and diseased plants collected and studied in the laboratory. Surprisingly, five different corticioid fungi were found associated with the crowns and roots of declining tussocks. Several species of Fusarium, belonging to four different species complexes, were also isolated from crowns and/or roots of declining tussocks, while Bipolaris sp. was isolated from a seed of N. trichotoma recovered from a burial trial within a patch of diseased plants. Artificial inoculations with all the obtained isolates were carried out on healthy N. trichotoma plantlets. After many failed inoculation experiments, it was concluded that the corticioid fungi were not acting as pathogens but rather as secondary colonisers on senescing plant material. In contrast, all the plantlets inoculated with Bipolaris sp. and Fusarium spp. developed some degree of crown and root discoloration and rot, and the inoculated fungi were all re-isolated from affected tissues. Because these pathogens were also found associated with seeds recovered from the soil around diseased plants, healthy seeds were inoculated with Bipolaris sp. and one Fusarium isolate in a separate experiment, causing a great decrease in their germination compared with un-inoculated ones. Surviving seedlings were less vigorous than those grown from healthy seeds. Overall, although all the tested isolates (other than the corticioid ones) were shown to cause some level of disease, none of them produced the die-back symptoms observed in the field. It is now presumed those are caused by more than one of these pathogens acting together in an additive or synergic manner. Moreover, at least two of them have been shown to have an impact on plant recruitment processes. More work is needed to clarify the etiology of this disease in Argentina and to fully assess the suitability of the involved fungi as biocontrol agents.
Fil: Angeletti, Bárbara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Daddario, Juan Facundo Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Loydi, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Anderson, Freda Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
XVI International Symposium on Biological Control of Weeds
Iguazú
Argentina
Fundación para el estudio de especies invasivas
Centro de Recursos Naturales Renovables de la Zona Semiárida
description Nassella trichotoma, a grass species native to South America, has become a noxious weed in many regions of the world. In Australia and New Zealand, it has been targeted for classical biological control. Initial studies to identify potential fungal biocontrol agents started in 1999 in Argentina and ended three years later having discarded a rust and a smut fungus. Work on a crown rot associated with tussock die-back symptoms, and believed to be caused by a corticioid fungus at the time, was less detailed, and abandoned with inconclusive results. Almost 20 years later the problem is being revisited, with research focused on plants with die-back symptoms. New surveys have been conducted and diseased plants collected and studied in the laboratory. Surprisingly, five different corticioid fungi were found associated with the crowns and roots of declining tussocks. Several species of Fusarium, belonging to four different species complexes, were also isolated from crowns and/or roots of declining tussocks, while Bipolaris sp. was isolated from a seed of N. trichotoma recovered from a burial trial within a patch of diseased plants. Artificial inoculations with all the obtained isolates were carried out on healthy N. trichotoma plantlets. After many failed inoculation experiments, it was concluded that the corticioid fungi were not acting as pathogens but rather as secondary colonisers on senescing plant material. In contrast, all the plantlets inoculated with Bipolaris sp. and Fusarium spp. developed some degree of crown and root discoloration and rot, and the inoculated fungi were all re-isolated from affected tissues. Because these pathogens were also found associated with seeds recovered from the soil around diseased plants, healthy seeds were inoculated with Bipolaris sp. and one Fusarium isolate in a separate experiment, causing a great decrease in their germination compared with un-inoculated ones. Surviving seedlings were less vigorous than those grown from healthy seeds. Overall, although all the tested isolates (other than the corticioid ones) were shown to cause some level of disease, none of them produced the die-back symptoms observed in the field. It is now presumed those are caused by more than one of these pathogens acting together in an additive or synergic manner. Moreover, at least two of them have been shown to have an impact on plant recruitment processes. More work is needed to clarify the etiology of this disease in Argentina and to fully assess the suitability of the involved fungi as biocontrol agents.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/222689
Nassella trichotoma (Poales:Poaceae) crown and root rot diseases in Argentina revisited: preliminary results; XVI International Symposium on Biological Control of Weeds; Iguazú; Argentina; 2023; 48-48
978-631-90256-0-6
CONICET Digital
CONICET
url http://hdl.handle.net/11336/222689
identifier_str_mv Nassella trichotoma (Poales:Poaceae) crown and root rot diseases in Argentina revisited: preliminary results; XVI International Symposium on Biological Control of Weeds; Iguazú; Argentina; 2023; 48-48
978-631-90256-0-6
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.iobc-global.org/global_sg_Classical_Weed_BC.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv International Symposium on biological control of weeds
publisher.none.fl_str_mv International Symposium on biological control of weeds
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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