The Microbial Indicators of Cover Crops in High N-Input Agroecosystems

Autores
Kim, Nakian; Riggins, Chance; Zabaloy, Maria Celina; Allegrini, Marco; Rodriguez Zas, Sandra; Villamil, Maria Bonita
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Cover cropping is anticipated for its ability to mitigate soil N loss from heavy nitrogen (N) fertilizer uses. However, despite the critical roles that the soil microbiota, there is a lack of indicators with higher taxonomic resolutions that well describe the soil microbial responses to the cover crops under high N input agroecosystem. Thus, our goal was to identify genus-level indicators of the soil microbial community responding to N fertilization and introduction of cover crops. A 3x2 split-plot arrangement of N fertilization rates (0, 202, 269 kg N/ha) and cover cropping (cover crops vs. bare fallow) treatments under continuous corn was studied in a randomized complete block design with three replicates over two years. Bacterial and archaeal 16S rRNA and fungal ITS regions were sequenced with Illumina MiSeq system, processed with QIIME 2.0, and classified using the Ribosomal Database Project (RDP) database. Acidophilic indicators increased in abundance with N fertilization while neutrophiles and alkaliphiles increase with unfertilized control. We found N fertilization increasing the abundances of indicators of nitrate (NO3-) reducers, and nitrite (NO2-) oxidizers. In unfertilized control, we detected greater abundances of heterotrophic nitrifiers, NO2- oxidizers, and complete denitrifiers. The bioindicators of unfertilized soils implied adaptation to N-poor condition, and N cycling with microbially reduced risk of N loss as nitrous oxide (N2O) and NO3-. More indicators with diverse niches associated with cover crops than bare fallow, including the N-fixing Mesorhizobium, and potentially mycorrhizal Albatrellus. However, cover cropping decreased the abundances of indicators of anaerobic ammonia oxidizers and N2O reducers, while increasing that of NO2- reducers. These indicators suggested higher risk of microbially mediated N2O emission with N abundance. This study provides the primary information on the soil microbial aspects of the cover cropping that is necessary for better evaluating its ability to reduce soil N loss.
Fil: Kim, Nakian. University of Illinois; Estados Unidos
Fil: Riggins, Chance. University of Illinois; Estados Unidos
Fil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Allegrini, Marco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Rodriguez Zas, Sandra. University of Illinois; Estados Unidos
Fil: Villamil, Maria Bonita. University of Illinois; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
ASA, CSSA, SSSA International Annual Meeting: A Creative Economy for Sustainable Development
Salt Lake City
Estados Unidos
American Society of Agronomy; Crop Science Society of America
Soil Science Society of America
Materia
COVER CROPS
N FERTILIZATION
FUNGAL DIVERSITY
BACTERIAL DIVERSITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/262075
Acceder
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network_name_str CONICET Digital (CONICET)
spelling The Microbial Indicators of Cover Crops in High N-Input AgroecosystemsKim, NakianRiggins, ChanceZabaloy, Maria CelinaAllegrini, MarcoRodriguez Zas, SandraVillamil, Maria BonitaCOVER CROPSN FERTILIZATIONFUNGAL DIVERSITYBACTERIAL DIVERSITYhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Cover cropping is anticipated for its ability to mitigate soil N loss from heavy nitrogen (N) fertilizer uses. However, despite the critical roles that the soil microbiota, there is a lack of indicators with higher taxonomic resolutions that well describe the soil microbial responses to the cover crops under high N input agroecosystem. Thus, our goal was to identify genus-level indicators of the soil microbial community responding to N fertilization and introduction of cover crops. A 3x2 split-plot arrangement of N fertilization rates (0, 202, 269 kg N/ha) and cover cropping (cover crops vs. bare fallow) treatments under continuous corn was studied in a randomized complete block design with three replicates over two years. Bacterial and archaeal 16S rRNA and fungal ITS regions were sequenced with Illumina MiSeq system, processed with QIIME 2.0, and classified using the Ribosomal Database Project (RDP) database. Acidophilic indicators increased in abundance with N fertilization while neutrophiles and alkaliphiles increase with unfertilized control. We found N fertilization increasing the abundances of indicators of nitrate (NO3-) reducers, and nitrite (NO2-) oxidizers. In unfertilized control, we detected greater abundances of heterotrophic nitrifiers, NO2- oxidizers, and complete denitrifiers. The bioindicators of unfertilized soils implied adaptation to N-poor condition, and N cycling with microbially reduced risk of N loss as nitrous oxide (N2O) and NO3-. More indicators with diverse niches associated with cover crops than bare fallow, including the N-fixing Mesorhizobium, and potentially mycorrhizal Albatrellus. However, cover cropping decreased the abundances of indicators of anaerobic ammonia oxidizers and N2O reducers, while increasing that of NO2- reducers. These indicators suggested higher risk of microbially mediated N2O emission with N abundance. This study provides the primary information on the soil microbial aspects of the cover cropping that is necessary for better evaluating its ability to reduce soil N loss.Fil: Kim, Nakian. University of Illinois; Estados UnidosFil: Riggins, Chance. University of Illinois; Estados UnidosFil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Allegrini, Marco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Rodriguez Zas, Sandra. University of Illinois; Estados UnidosFil: Villamil, Maria Bonita. University of Illinois; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaASA, CSSA, SSSA International Annual Meeting: A Creative Economy for Sustainable DevelopmentSalt Lake CityEstados UnidosAmerican Society of Agronomy; Crop Science Society of AmericaSoil Science Society of AmericaAmerican Society of Agronomy2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/262075The Microbial Indicators of Cover Crops in High N-Input Agroecosystems; ASA, CSSA, SSSA International Annual Meeting: A Creative Economy for Sustainable Development; Salt Lake City; Estados Unidos; 2021; 1-3CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://scisoc.confex.com/scisoc/2021am/meetingapp.cgi/Paper/135544Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:34:09Zoai:ri.conicet.gov.ar:11336/262075instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:34:09.734CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The Microbial Indicators of Cover Crops in High N-Input Agroecosystems
title The Microbial Indicators of Cover Crops in High N-Input Agroecosystems
spellingShingle The Microbial Indicators of Cover Crops in High N-Input Agroecosystems
Kim, Nakian
COVER CROPS
N FERTILIZATION
FUNGAL DIVERSITY
BACTERIAL DIVERSITY
title_short The Microbial Indicators of Cover Crops in High N-Input Agroecosystems
title_full The Microbial Indicators of Cover Crops in High N-Input Agroecosystems
title_fullStr The Microbial Indicators of Cover Crops in High N-Input Agroecosystems
title_full_unstemmed The Microbial Indicators of Cover Crops in High N-Input Agroecosystems
title_sort The Microbial Indicators of Cover Crops in High N-Input Agroecosystems
dc.creator.none.fl_str_mv Kim, Nakian
Riggins, Chance
Zabaloy, Maria Celina
Allegrini, Marco
Rodriguez Zas, Sandra
Villamil, Maria Bonita
author Kim, Nakian
author_facet Kim, Nakian
Riggins, Chance
Zabaloy, Maria Celina
Allegrini, Marco
Rodriguez Zas, Sandra
Villamil, Maria Bonita
author_role author
author2 Riggins, Chance
Zabaloy, Maria Celina
Allegrini, Marco
Rodriguez Zas, Sandra
Villamil, Maria Bonita
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv COVER CROPS
N FERTILIZATION
FUNGAL DIVERSITY
BACTERIAL DIVERSITY
topic COVER CROPS
N FERTILIZATION
FUNGAL DIVERSITY
BACTERIAL DIVERSITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Cover cropping is anticipated for its ability to mitigate soil N loss from heavy nitrogen (N) fertilizer uses. However, despite the critical roles that the soil microbiota, there is a lack of indicators with higher taxonomic resolutions that well describe the soil microbial responses to the cover crops under high N input agroecosystem. Thus, our goal was to identify genus-level indicators of the soil microbial community responding to N fertilization and introduction of cover crops. A 3x2 split-plot arrangement of N fertilization rates (0, 202, 269 kg N/ha) and cover cropping (cover crops vs. bare fallow) treatments under continuous corn was studied in a randomized complete block design with three replicates over two years. Bacterial and archaeal 16S rRNA and fungal ITS regions were sequenced with Illumina MiSeq system, processed with QIIME 2.0, and classified using the Ribosomal Database Project (RDP) database. Acidophilic indicators increased in abundance with N fertilization while neutrophiles and alkaliphiles increase with unfertilized control. We found N fertilization increasing the abundances of indicators of nitrate (NO3-) reducers, and nitrite (NO2-) oxidizers. In unfertilized control, we detected greater abundances of heterotrophic nitrifiers, NO2- oxidizers, and complete denitrifiers. The bioindicators of unfertilized soils implied adaptation to N-poor condition, and N cycling with microbially reduced risk of N loss as nitrous oxide (N2O) and NO3-. More indicators with diverse niches associated with cover crops than bare fallow, including the N-fixing Mesorhizobium, and potentially mycorrhizal Albatrellus. However, cover cropping decreased the abundances of indicators of anaerobic ammonia oxidizers and N2O reducers, while increasing that of NO2- reducers. These indicators suggested higher risk of microbially mediated N2O emission with N abundance. This study provides the primary information on the soil microbial aspects of the cover cropping that is necessary for better evaluating its ability to reduce soil N loss.
Fil: Kim, Nakian. University of Illinois; Estados Unidos
Fil: Riggins, Chance. University of Illinois; Estados Unidos
Fil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Allegrini, Marco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Rodriguez Zas, Sandra. University of Illinois; Estados Unidos
Fil: Villamil, Maria Bonita. University of Illinois; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
ASA, CSSA, SSSA International Annual Meeting: A Creative Economy for Sustainable Development
Salt Lake City
Estados Unidos
American Society of Agronomy; Crop Science Society of America
Soil Science Society of America
description Cover cropping is anticipated for its ability to mitigate soil N loss from heavy nitrogen (N) fertilizer uses. However, despite the critical roles that the soil microbiota, there is a lack of indicators with higher taxonomic resolutions that well describe the soil microbial responses to the cover crops under high N input agroecosystem. Thus, our goal was to identify genus-level indicators of the soil microbial community responding to N fertilization and introduction of cover crops. A 3x2 split-plot arrangement of N fertilization rates (0, 202, 269 kg N/ha) and cover cropping (cover crops vs. bare fallow) treatments under continuous corn was studied in a randomized complete block design with three replicates over two years. Bacterial and archaeal 16S rRNA and fungal ITS regions were sequenced with Illumina MiSeq system, processed with QIIME 2.0, and classified using the Ribosomal Database Project (RDP) database. Acidophilic indicators increased in abundance with N fertilization while neutrophiles and alkaliphiles increase with unfertilized control. We found N fertilization increasing the abundances of indicators of nitrate (NO3-) reducers, and nitrite (NO2-) oxidizers. In unfertilized control, we detected greater abundances of heterotrophic nitrifiers, NO2- oxidizers, and complete denitrifiers. The bioindicators of unfertilized soils implied adaptation to N-poor condition, and N cycling with microbially reduced risk of N loss as nitrous oxide (N2O) and NO3-. More indicators with diverse niches associated with cover crops than bare fallow, including the N-fixing Mesorhizobium, and potentially mycorrhizal Albatrellus. However, cover cropping decreased the abundances of indicators of anaerobic ammonia oxidizers and N2O reducers, while increasing that of NO2- reducers. These indicators suggested higher risk of microbially mediated N2O emission with N abundance. This study provides the primary information on the soil microbial aspects of the cover cropping that is necessary for better evaluating its ability to reduce soil N loss.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/262075
The Microbial Indicators of Cover Crops in High N-Input Agroecosystems; ASA, CSSA, SSSA International Annual Meeting: A Creative Economy for Sustainable Development; Salt Lake City; Estados Unidos; 2021; 1-3
CONICET Digital
CONICET
url http://hdl.handle.net/11336/262075
identifier_str_mv The Microbial Indicators of Cover Crops in High N-Input Agroecosystems; ASA, CSSA, SSSA International Annual Meeting: A Creative Economy for Sustainable Development; Salt Lake City; Estados Unidos; 2021; 1-3
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://scisoc.confex.com/scisoc/2021am/meetingapp.cgi/Paper/135544
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv American Society of Agronomy
publisher.none.fl_str_mv American Society of Agronomy
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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