High-Resolution Indicators of Soil Microbial Responses to N Fertilization and Cover Cropping in Corn Monoculture

Autores
Kim, Nakian; Riggins, Chance W.; Zabaloy, Maria Celina; Allegrini, Marco; Rodriguez Zas, Sandra L.; Villamil, Maria Bonita
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cover cropping (CC) is the most promising in-field practice to improve soil health and mitigate N losses from fertilizer use. Although the soil microbiota play essential roles in soil health, their response to CC has not been well characterized by bioindicators of high taxonomic resolution within typical agricultural systems. Our objective was to fill this knowledge gap with genus-level indicators for corn [Zea mays L.] monocultures with three N fertilizer rates (N0, N202, N269; kg N ha−1), after introducing a CC mixture of cereal rye [Secale cereale L.] and hairy vetch [Vicia villosa Roth.], using winter fallows (BF) as controls. A 3 × 2 split-plot arrangement of N rates and CC treatments was studied in a randomized complete block design with three replicates over two years. Bacterial and archaeal 16S rRNA and fungal ITS regions were sequenced with Illumina MiSeq system. Overall, our high-resolution bioindicators were able to represent specific functional or ecological shifts within the microbial community. The abundances of indicators representing acidophiles, nitrifiers, and denitrifiers increased with N fertilization, while those of heterotrophic nitrifiers, nitrite oxidizers, and complete denitrifiers increased with N0. Introducing CC decreased soil nitrate levels by up to 50% across N rates, and CC biomass increased by 73% with N fertilization. CC promoted indicators of diverse functions and niches, including N-fixers, nitrite reducers, and mycorrhizae, while only two N-cycling genera were associated with BF. Thus, CC can enhance the soil biodiversity of simplified cropping systems and reduce nitrate leaching, but might increase the risk of nitrous oxide emission without proper nutrient management. This primary information is the first of its kind in this system and provided valuable insights into the limits and potential of CC as a strategy to improve soil health.
Fil: Kim, Nakian. University of Illinois at Urbana; Estados Unidos
Fil: Riggins, Chance W.. University of Illinois at Urbana; Estados Unidos
Fil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Allegrini, Marco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Rodriguez Zas, Sandra L.. University of Illinois at Urbana; Estados Unidos
Fil: Villamil, Maria Bonita. University of Illinois at Urbana; Estados Unidos
Materia
BIOINFORMATICS
MAIZE
NITRATE LEACHING
NITROGEN CYCLING
NITROUS OXIDE EMISSION
SOIL MICROBIOTA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/197040
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/197040
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling High-Resolution Indicators of Soil Microbial Responses to N Fertilization and Cover Cropping in Corn MonocultureKim, NakianRiggins, Chance W.Zabaloy, Maria CelinaAllegrini, MarcoRodriguez Zas, Sandra L.Villamil, Maria BonitaBIOINFORMATICSMAIZENITRATE LEACHINGNITROGEN CYCLINGNITROUS OXIDE EMISSIONSOIL MICROBIOTAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Cover cropping (CC) is the most promising in-field practice to improve soil health and mitigate N losses from fertilizer use. Although the soil microbiota play essential roles in soil health, their response to CC has not been well characterized by bioindicators of high taxonomic resolution within typical agricultural systems. Our objective was to fill this knowledge gap with genus-level indicators for corn [Zea mays L.] monocultures with three N fertilizer rates (N0, N202, N269; kg N ha−1), after introducing a CC mixture of cereal rye [Secale cereale L.] and hairy vetch [Vicia villosa Roth.], using winter fallows (BF) as controls. A 3 × 2 split-plot arrangement of N rates and CC treatments was studied in a randomized complete block design with three replicates over two years. Bacterial and archaeal 16S rRNA and fungal ITS regions were sequenced with Illumina MiSeq system. Overall, our high-resolution bioindicators were able to represent specific functional or ecological shifts within the microbial community. The abundances of indicators representing acidophiles, nitrifiers, and denitrifiers increased with N fertilization, while those of heterotrophic nitrifiers, nitrite oxidizers, and complete denitrifiers increased with N0. Introducing CC decreased soil nitrate levels by up to 50% across N rates, and CC biomass increased by 73% with N fertilization. CC promoted indicators of diverse functions and niches, including N-fixers, nitrite reducers, and mycorrhizae, while only two N-cycling genera were associated with BF. Thus, CC can enhance the soil biodiversity of simplified cropping systems and reduce nitrate leaching, but might increase the risk of nitrous oxide emission without proper nutrient management. This primary information is the first of its kind in this system and provided valuable insights into the limits and potential of CC as a strategy to improve soil health.Fil: Kim, Nakian. University of Illinois at Urbana; Estados UnidosFil: Riggins, Chance W.. University of Illinois at Urbana; Estados UnidosFil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Allegrini, Marco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Rodriguez Zas, Sandra L.. University of Illinois at Urbana; Estados UnidosFil: Villamil, Maria Bonita. University of Illinois at Urbana; Estados UnidosMDPI2022-04-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/197040Kim, Nakian; Riggins, Chance W.; Zabaloy, Maria Celina; Allegrini, Marco; Rodriguez Zas, Sandra L.; et al.; High-Resolution Indicators of Soil Microbial Responses to N Fertilization and Cover Cropping in Corn Monoculture; MDPI; Agronomy; 12; 4; 15-4-2022; 1-262073-4395CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4395/12/4/954info:eu-repo/semantics/altIdentifier/doi/10.3390/agronomy12040954info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:24:56Zoai:ri.conicet.gov.ar:11336/197040instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:24:57.158CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv High-Resolution Indicators of Soil Microbial Responses to N Fertilization and Cover Cropping in Corn Monoculture
title High-Resolution Indicators of Soil Microbial Responses to N Fertilization and Cover Cropping in Corn Monoculture
spellingShingle High-Resolution Indicators of Soil Microbial Responses to N Fertilization and Cover Cropping in Corn Monoculture
Kim, Nakian
BIOINFORMATICS
MAIZE
NITRATE LEACHING
NITROGEN CYCLING
NITROUS OXIDE EMISSION
SOIL MICROBIOTA
title_short High-Resolution Indicators of Soil Microbial Responses to N Fertilization and Cover Cropping in Corn Monoculture
title_full High-Resolution Indicators of Soil Microbial Responses to N Fertilization and Cover Cropping in Corn Monoculture
title_fullStr High-Resolution Indicators of Soil Microbial Responses to N Fertilization and Cover Cropping in Corn Monoculture
title_full_unstemmed High-Resolution Indicators of Soil Microbial Responses to N Fertilization and Cover Cropping in Corn Monoculture
title_sort High-Resolution Indicators of Soil Microbial Responses to N Fertilization and Cover Cropping in Corn Monoculture
dc.creator.none.fl_str_mv Kim, Nakian
Riggins, Chance W.
Zabaloy, Maria Celina
Allegrini, Marco
Rodriguez Zas, Sandra L.
Villamil, Maria Bonita
author Kim, Nakian
author_facet Kim, Nakian
Riggins, Chance W.
Zabaloy, Maria Celina
Allegrini, Marco
Rodriguez Zas, Sandra L.
Villamil, Maria Bonita
author_role author
author2 Riggins, Chance W.
Zabaloy, Maria Celina
Allegrini, Marco
Rodriguez Zas, Sandra L.
Villamil, Maria Bonita
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BIOINFORMATICS
MAIZE
NITRATE LEACHING
NITROGEN CYCLING
NITROUS OXIDE EMISSION
SOIL MICROBIOTA
topic BIOINFORMATICS
MAIZE
NITRATE LEACHING
NITROGEN CYCLING
NITROUS OXIDE EMISSION
SOIL MICROBIOTA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Cover cropping (CC) is the most promising in-field practice to improve soil health and mitigate N losses from fertilizer use. Although the soil microbiota play essential roles in soil health, their response to CC has not been well characterized by bioindicators of high taxonomic resolution within typical agricultural systems. Our objective was to fill this knowledge gap with genus-level indicators for corn [Zea mays L.] monocultures with three N fertilizer rates (N0, N202, N269; kg N ha−1), after introducing a CC mixture of cereal rye [Secale cereale L.] and hairy vetch [Vicia villosa Roth.], using winter fallows (BF) as controls. A 3 × 2 split-plot arrangement of N rates and CC treatments was studied in a randomized complete block design with three replicates over two years. Bacterial and archaeal 16S rRNA and fungal ITS regions were sequenced with Illumina MiSeq system. Overall, our high-resolution bioindicators were able to represent specific functional or ecological shifts within the microbial community. The abundances of indicators representing acidophiles, nitrifiers, and denitrifiers increased with N fertilization, while those of heterotrophic nitrifiers, nitrite oxidizers, and complete denitrifiers increased with N0. Introducing CC decreased soil nitrate levels by up to 50% across N rates, and CC biomass increased by 73% with N fertilization. CC promoted indicators of diverse functions and niches, including N-fixers, nitrite reducers, and mycorrhizae, while only two N-cycling genera were associated with BF. Thus, CC can enhance the soil biodiversity of simplified cropping systems and reduce nitrate leaching, but might increase the risk of nitrous oxide emission without proper nutrient management. This primary information is the first of its kind in this system and provided valuable insights into the limits and potential of CC as a strategy to improve soil health.
Fil: Kim, Nakian. University of Illinois at Urbana; Estados Unidos
Fil: Riggins, Chance W.. University of Illinois at Urbana; Estados Unidos
Fil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Allegrini, Marco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Rodriguez Zas, Sandra L.. University of Illinois at Urbana; Estados Unidos
Fil: Villamil, Maria Bonita. University of Illinois at Urbana; Estados Unidos
description Cover cropping (CC) is the most promising in-field practice to improve soil health and mitigate N losses from fertilizer use. Although the soil microbiota play essential roles in soil health, their response to CC has not been well characterized by bioindicators of high taxonomic resolution within typical agricultural systems. Our objective was to fill this knowledge gap with genus-level indicators for corn [Zea mays L.] monocultures with three N fertilizer rates (N0, N202, N269; kg N ha−1), after introducing a CC mixture of cereal rye [Secale cereale L.] and hairy vetch [Vicia villosa Roth.], using winter fallows (BF) as controls. A 3 × 2 split-plot arrangement of N rates and CC treatments was studied in a randomized complete block design with three replicates over two years. Bacterial and archaeal 16S rRNA and fungal ITS regions were sequenced with Illumina MiSeq system. Overall, our high-resolution bioindicators were able to represent specific functional or ecological shifts within the microbial community. The abundances of indicators representing acidophiles, nitrifiers, and denitrifiers increased with N fertilization, while those of heterotrophic nitrifiers, nitrite oxidizers, and complete denitrifiers increased with N0. Introducing CC decreased soil nitrate levels by up to 50% across N rates, and CC biomass increased by 73% with N fertilization. CC promoted indicators of diverse functions and niches, including N-fixers, nitrite reducers, and mycorrhizae, while only two N-cycling genera were associated with BF. Thus, CC can enhance the soil biodiversity of simplified cropping systems and reduce nitrate leaching, but might increase the risk of nitrous oxide emission without proper nutrient management. This primary information is the first of its kind in this system and provided valuable insights into the limits and potential of CC as a strategy to improve soil health.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/197040
Kim, Nakian; Riggins, Chance W.; Zabaloy, Maria Celina; Allegrini, Marco; Rodriguez Zas, Sandra L.; et al.; High-Resolution Indicators of Soil Microbial Responses to N Fertilization and Cover Cropping in Corn Monoculture; MDPI; Agronomy; 12; 4; 15-4-2022; 1-26
2073-4395
CONICET Digital
CONICET
url http://hdl.handle.net/11336/197040
identifier_str_mv Kim, Nakian; Riggins, Chance W.; Zabaloy, Maria Celina; Allegrini, Marco; Rodriguez Zas, Sandra L.; et al.; High-Resolution Indicators of Soil Microbial Responses to N Fertilization and Cover Cropping in Corn Monoculture; MDPI; Agronomy; 12; 4; 15-4-2022; 1-26
2073-4395
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4395/12/4/954
info:eu-repo/semantics/altIdentifier/doi/10.3390/agronomy12040954
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.087074

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