Amphetamine induced differential effects in vascular and glial components at somatosensory cortex: Why to focus on AT1 receptors

Autores
Armonelli Fiedler, Samanta; Occhieppo, Victoria Belen; Basmadjian, Osvaldo Martin; Bregonzio Diaz, Claudia; Baiardi, Gustavo Carlos
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Amphetamine (Amph), is associated with inflammatory processes, involving glial and vascular alterations. Brain Angiotensin II, through AT1-receptors (AT1-R), modulates dopaminergic neurotransmission and plays a crucial role in inflammatory responses. Our aim was to evaluate the role of AT1-R in long-term alterations induced by repeated exposure to Amph. Astrocyte and microglia reactivity, and brain microvascular network were analysed at the somatosensory cortex (S1 Barrel and S1 Trunk area). Male Wistar rats (250–320 g) were administered with AT1-R antagonist Candesartan/vehicle (3 mg/kg p.o., days 1–10) and Amph/saline (2.5 mg/kg i.p., days 6–10). The four experimental groups at the two times evaluated (17 and 31 days) were: Veh-Sal, CV-Sal, Veh-Amph, CV-Amph. On days 17 and 31 the animals were sacrificed and their brains were processed for immunohistochemistry against GFAP (astroglial marker), CD11b (microglial marker) and von Willebrand factor (vascular marker). Data were analysed with factorial ANOVA followed by Bonferroni test. Our results indicate that Amph exposure induces an endurable increase in astrocyte and microglia reactivity at S1 Barrel and S1 Trunk area. Although, the microvascular rearrangement (evaluated as vascular area density, branching points and tortuosity) showed time dependant differential response to Amph, since at day 31 these parameters return to basal conditions at S1 Barrel. Meanwhile, at S1 Trunk the vascular changes were observed only at day 31. Pretreatment with the AT1-R blocker prevented the described alterations induced by Amph. We conclude that neuroplastic changes induced by Amph demand an AT1-R active role showing a regional susceptibility at vascular level.
Fil: Armonelli Fiedler, Samanta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Occhieppo, Victoria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Basmadjian, Osvaldo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Bregonzio Diaz, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Baiardi, Gustavo Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología y IX Reunión Anual de la Asociación Argentina de Nanomedicinas
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Materia
AT1 receptors
Amphetamine
Brain vasculature
Astroglia
Microglia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/154293
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/154293
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Amphetamine induced differential effects in vascular and glial components at somatosensory cortex: Why to focus on AT1 receptorsArmonelli Fiedler, SamantaOcchieppo, Victoria BelenBasmadjian, Osvaldo MartinBregonzio Diaz, ClaudiaBaiardi, Gustavo CarlosAT1 receptorsAmphetamineBrain vasculatureAstrogliaMicrogliahttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Amphetamine (Amph), is associated with inflammatory processes, involving glial and vascular alterations. Brain Angiotensin II, through AT1-receptors (AT1-R), modulates dopaminergic neurotransmission and plays a crucial role in inflammatory responses. Our aim was to evaluate the role of AT1-R in long-term alterations induced by repeated exposure to Amph. Astrocyte and microglia reactivity, and brain microvascular network were analysed at the somatosensory cortex (S1 Barrel and S1 Trunk area). Male Wistar rats (250–320 g) were administered with AT1-R antagonist Candesartan/vehicle (3 mg/kg p.o., days 1–10) and Amph/saline (2.5 mg/kg i.p., days 6–10). The four experimental groups at the two times evaluated (17 and 31 days) were: Veh-Sal, CV-Sal, Veh-Amph, CV-Amph. On days 17 and 31 the animals were sacrificed and their brains were processed for immunohistochemistry against GFAP (astroglial marker), CD11b (microglial marker) and von Willebrand factor (vascular marker). Data were analysed with factorial ANOVA followed by Bonferroni test. Our results indicate that Amph exposure induces an endurable increase in astrocyte and microglia reactivity at S1 Barrel and S1 Trunk area. Although, the microvascular rearrangement (evaluated as vascular area density, branching points and tortuosity) showed time dependant differential response to Amph, since at day 31 these parameters return to basal conditions at S1 Barrel. Meanwhile, at S1 Trunk the vascular changes were observed only at day 31. Pretreatment with the AT1-R blocker prevented the described alterations induced by Amph. We conclude that neuroplastic changes induced by Amph demand an AT1-R active role showing a regional susceptibility at vascular level.Fil: Armonelli Fiedler, Samanta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; ArgentinaFil: Occhieppo, Victoria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; ArgentinaFil: Basmadjian, Osvaldo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; ArgentinaFil: Bregonzio Diaz, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; ArgentinaFil: Baiardi, Gustavo Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaLXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología y IX Reunión Anual de la Asociación Argentina de NanomedicinasMar del PlataArgentinaSociedad Argentina de Investigación ClínicaAsociación Argentina de Farmacología ExperimentalSociedad Argentina de BiologíaSociedad Argentina de ProtozoologíaAsociación Argentina de NanomedicinasAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioFundación Revista MedicinaCostas, Monica AlejandraMarino, Gabriela InésAzurmendi, Pablo Javier2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/154293Amphetamine induced differential effects in vascular and glial components at somatosensory cortex: Why to focus on AT1 receptors; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología y IX Reunión Anual de la Asociación Argentina de Nanomedicinas; Mar del Plata; Argentina; 2019; 115-1150025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2010-a-2019/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:04:45Zoai:ri.conicet.gov.ar:11336/154293instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:04:45.796CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Amphetamine induced differential effects in vascular and glial components at somatosensory cortex: Why to focus on AT1 receptors
title Amphetamine induced differential effects in vascular and glial components at somatosensory cortex: Why to focus on AT1 receptors
spellingShingle Amphetamine induced differential effects in vascular and glial components at somatosensory cortex: Why to focus on AT1 receptors
Armonelli Fiedler, Samanta
AT1 receptors
Amphetamine
Brain vasculature
Astroglia
Microglia
title_short Amphetamine induced differential effects in vascular and glial components at somatosensory cortex: Why to focus on AT1 receptors
title_full Amphetamine induced differential effects in vascular and glial components at somatosensory cortex: Why to focus on AT1 receptors
title_fullStr Amphetamine induced differential effects in vascular and glial components at somatosensory cortex: Why to focus on AT1 receptors
title_full_unstemmed Amphetamine induced differential effects in vascular and glial components at somatosensory cortex: Why to focus on AT1 receptors
title_sort Amphetamine induced differential effects in vascular and glial components at somatosensory cortex: Why to focus on AT1 receptors
dc.creator.none.fl_str_mv Armonelli Fiedler, Samanta
Occhieppo, Victoria Belen
Basmadjian, Osvaldo Martin
Bregonzio Diaz, Claudia
Baiardi, Gustavo Carlos
author Armonelli Fiedler, Samanta
author_facet Armonelli Fiedler, Samanta
Occhieppo, Victoria Belen
Basmadjian, Osvaldo Martin
Bregonzio Diaz, Claudia
Baiardi, Gustavo Carlos
author_role author
author2 Occhieppo, Victoria Belen
Basmadjian, Osvaldo Martin
Bregonzio Diaz, Claudia
Baiardi, Gustavo Carlos
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Costas, Monica Alejandra
Marino, Gabriela Inés
Azurmendi, Pablo Javier
dc.subject.none.fl_str_mv AT1 receptors
Amphetamine
Brain vasculature
Astroglia
Microglia
topic AT1 receptors
Amphetamine
Brain vasculature
Astroglia
Microglia
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Amphetamine (Amph), is associated with inflammatory processes, involving glial and vascular alterations. Brain Angiotensin II, through AT1-receptors (AT1-R), modulates dopaminergic neurotransmission and plays a crucial role in inflammatory responses. Our aim was to evaluate the role of AT1-R in long-term alterations induced by repeated exposure to Amph. Astrocyte and microglia reactivity, and brain microvascular network were analysed at the somatosensory cortex (S1 Barrel and S1 Trunk area). Male Wistar rats (250–320 g) were administered with AT1-R antagonist Candesartan/vehicle (3 mg/kg p.o., days 1–10) and Amph/saline (2.5 mg/kg i.p., days 6–10). The four experimental groups at the two times evaluated (17 and 31 days) were: Veh-Sal, CV-Sal, Veh-Amph, CV-Amph. On days 17 and 31 the animals were sacrificed and their brains were processed for immunohistochemistry against GFAP (astroglial marker), CD11b (microglial marker) and von Willebrand factor (vascular marker). Data were analysed with factorial ANOVA followed by Bonferroni test. Our results indicate that Amph exposure induces an endurable increase in astrocyte and microglia reactivity at S1 Barrel and S1 Trunk area. Although, the microvascular rearrangement (evaluated as vascular area density, branching points and tortuosity) showed time dependant differential response to Amph, since at day 31 these parameters return to basal conditions at S1 Barrel. Meanwhile, at S1 Trunk the vascular changes were observed only at day 31. Pretreatment with the AT1-R blocker prevented the described alterations induced by Amph. We conclude that neuroplastic changes induced by Amph demand an AT1-R active role showing a regional susceptibility at vascular level.
Fil: Armonelli Fiedler, Samanta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Occhieppo, Victoria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Basmadjian, Osvaldo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Bregonzio Diaz, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Baiardi, Gustavo Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología y IX Reunión Anual de la Asociación Argentina de Nanomedicinas
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
description Amphetamine (Amph), is associated with inflammatory processes, involving glial and vascular alterations. Brain Angiotensin II, through AT1-receptors (AT1-R), modulates dopaminergic neurotransmission and plays a crucial role in inflammatory responses. Our aim was to evaluate the role of AT1-R in long-term alterations induced by repeated exposure to Amph. Astrocyte and microglia reactivity, and brain microvascular network were analysed at the somatosensory cortex (S1 Barrel and S1 Trunk area). Male Wistar rats (250–320 g) were administered with AT1-R antagonist Candesartan/vehicle (3 mg/kg p.o., days 1–10) and Amph/saline (2.5 mg/kg i.p., days 6–10). The four experimental groups at the two times evaluated (17 and 31 days) were: Veh-Sal, CV-Sal, Veh-Amph, CV-Amph. On days 17 and 31 the animals were sacrificed and their brains were processed for immunohistochemistry against GFAP (astroglial marker), CD11b (microglial marker) and von Willebrand factor (vascular marker). Data were analysed with factorial ANOVA followed by Bonferroni test. Our results indicate that Amph exposure induces an endurable increase in astrocyte and microglia reactivity at S1 Barrel and S1 Trunk area. Although, the microvascular rearrangement (evaluated as vascular area density, branching points and tortuosity) showed time dependant differential response to Amph, since at day 31 these parameters return to basal conditions at S1 Barrel. Meanwhile, at S1 Trunk the vascular changes were observed only at day 31. Pretreatment with the AT1-R blocker prevented the described alterations induced by Amph. We conclude that neuroplastic changes induced by Amph demand an AT1-R active role showing a regional susceptibility at vascular level.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/154293
Amphetamine induced differential effects in vascular and glial components at somatosensory cortex: Why to focus on AT1 receptors; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología y IX Reunión Anual de la Asociación Argentina de Nanomedicinas; Mar del Plata; Argentina; 2019; 115-115
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/154293
identifier_str_mv Amphetamine induced differential effects in vascular and glial components at somatosensory cortex: Why to focus on AT1 receptors; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología y IX Reunión Anual de la Asociación Argentina de Nanomedicinas; Mar del Plata; Argentina; 2019; 115-115
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2010-a-2019/
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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