Wavelet analysis of corneal endothelial electrical potential difference reveals cyclic operation of the secretory mechanism

Autores
Cacace, Verónica Inés; Montalbetti, Nicolas; Kusnier, Carlos Federico; Gomez, M. P.; Fischbarg, Jorge
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The corneal endothelium is a fluid-transporting epithelium. As other similar tissues, it displays an electrical potential of ∼1 mV (aqueous side negative) across the entire layer [transendothelial potential difference (TEPD)]. It appears that this electrical potential is mainly the result of the transport of anions across the cell layer (from stroma to aqueous). There is substantial evidence that the TEPD is related linearly to fluid transport; hence, under proper conditions, its measure could serve as a measure of fluid transport. Furthermore, the TEPD is not steady; instead, it displays a spectrum of frequency components (0-15 Hz) recognized recently using Fourier transforms. Such frequency components appear due to charge-separating (electrogenic) processes mediated by epithelial plasma membrane proteins (both ionic channels and ionic cotransporters). In particular, the endothelial TEPD oscillations of the highest amplitude (1-2 Hz) were linked to the operation of so-called sodium bicarbonate cotransporters. However, no time localization of that activity could be obtained with the Fourier methodology utilized. For that reason we now characterize the TEPD using wavelet analysis with the aim to localize in time the variations in TEPD. We find that the mentioned high-amplitude oscillatory components of the TEPD appear cyclically during the several hours that an endothelial preparation survives in vitro. They have a period of 4.6 ± 0.4 s on average (n=4). The wavelet power value at the peak of such oscillations is 1.5 ± 0.1 mV2 Hz on average (n = 4), and is remarkably narrow in its distribution.
Fil: Cacace, Verónica Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Montalbetti, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Kusnier, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Gomez, M. P.. Comisión Nacional de Energía Atómica; Argentina. Universidad Tecnológica Nacional; Argentina
Fil: Fischbarg, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Materia
Wavelet
Epithelia
Electrical
Potential
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67464
Acceder
id CONICETDig_f016c4321aab1adbd46051e9f15724b5
oai_identifier_str oai:ri.conicet.gov.ar:11336/67464
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Wavelet analysis of corneal endothelial electrical potential difference reveals cyclic operation of the secretory mechanismCacace, Verónica InésMontalbetti, NicolasKusnier, Carlos FedericoGomez, M. P.Fischbarg, JorgeWaveletEpitheliaElectricalPotentialhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The corneal endothelium is a fluid-transporting epithelium. As other similar tissues, it displays an electrical potential of ∼1 mV (aqueous side negative) across the entire layer [transendothelial potential difference (TEPD)]. It appears that this electrical potential is mainly the result of the transport of anions across the cell layer (from stroma to aqueous). There is substantial evidence that the TEPD is related linearly to fluid transport; hence, under proper conditions, its measure could serve as a measure of fluid transport. Furthermore, the TEPD is not steady; instead, it displays a spectrum of frequency components (0-15 Hz) recognized recently using Fourier transforms. Such frequency components appear due to charge-separating (electrogenic) processes mediated by epithelial plasma membrane proteins (both ionic channels and ionic cotransporters). In particular, the endothelial TEPD oscillations of the highest amplitude (1-2 Hz) were linked to the operation of so-called sodium bicarbonate cotransporters. However, no time localization of that activity could be obtained with the Fourier methodology utilized. For that reason we now characterize the TEPD using wavelet analysis with the aim to localize in time the variations in TEPD. We find that the mentioned high-amplitude oscillatory components of the TEPD appear cyclically during the several hours that an endothelial preparation survives in vitro. They have a period of 4.6 ± 0.4 s on average (n=4). The wavelet power value at the peak of such oscillations is 1.5 ± 0.1 mV2 Hz on average (n = 4), and is remarkably narrow in its distribution.Fil: Cacace, Verónica Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Montalbetti, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Kusnier, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Gomez, M. P.. Comisión Nacional de Energía Atómica; Argentina. Universidad Tecnológica Nacional; ArgentinaFil: Fischbarg, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaAmerican Physical Society2011-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67464Cacace, Verónica Inés; Montalbetti, Nicolas; Kusnier, Carlos Federico; Gomez, M. P.; Fischbarg, Jorge; Wavelet analysis of corneal endothelial electrical potential difference reveals cyclic operation of the secretory mechanism; American Physical Society; Physical Review E: Statistical, Nonlinear and Soft Matter Physics; 84; 3; 9-2011; 1-5; 0329021539-3755CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1103/PhysRevE.84.032902info:eu-repo/semantics/altIdentifier/url/https://journals.aps.org/pre/abstract/10.1103/PhysRevE.84.032902info:eu-repo/semantics/altIdentifier/url/https://arxiv.org/abs/1106.3053info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:09Zoai:ri.conicet.gov.ar:11336/67464instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:10.189CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Wavelet analysis of corneal endothelial electrical potential difference reveals cyclic operation of the secretory mechanism
title Wavelet analysis of corneal endothelial electrical potential difference reveals cyclic operation of the secretory mechanism
spellingShingle Wavelet analysis of corneal endothelial electrical potential difference reveals cyclic operation of the secretory mechanism
Cacace, Verónica Inés
Wavelet
Epithelia
Electrical
Potential
title_short Wavelet analysis of corneal endothelial electrical potential difference reveals cyclic operation of the secretory mechanism
title_full Wavelet analysis of corneal endothelial electrical potential difference reveals cyclic operation of the secretory mechanism
title_fullStr Wavelet analysis of corneal endothelial electrical potential difference reveals cyclic operation of the secretory mechanism
title_full_unstemmed Wavelet analysis of corneal endothelial electrical potential difference reveals cyclic operation of the secretory mechanism
title_sort Wavelet analysis of corneal endothelial electrical potential difference reveals cyclic operation of the secretory mechanism
dc.creator.none.fl_str_mv Cacace, Verónica Inés
Montalbetti, Nicolas
Kusnier, Carlos Federico
Gomez, M. P.
Fischbarg, Jorge
author Cacace, Verónica Inés
author_facet Cacace, Verónica Inés
Montalbetti, Nicolas
Kusnier, Carlos Federico
Gomez, M. P.
Fischbarg, Jorge
author_role author
author2 Montalbetti, Nicolas
Kusnier, Carlos Federico
Gomez, M. P.
Fischbarg, Jorge
author2_role author
author
author
author
dc.subject.none.fl_str_mv Wavelet
Epithelia
Electrical
Potential
topic Wavelet
Epithelia
Electrical
Potential
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The corneal endothelium is a fluid-transporting epithelium. As other similar tissues, it displays an electrical potential of ∼1 mV (aqueous side negative) across the entire layer [transendothelial potential difference (TEPD)]. It appears that this electrical potential is mainly the result of the transport of anions across the cell layer (from stroma to aqueous). There is substantial evidence that the TEPD is related linearly to fluid transport; hence, under proper conditions, its measure could serve as a measure of fluid transport. Furthermore, the TEPD is not steady; instead, it displays a spectrum of frequency components (0-15 Hz) recognized recently using Fourier transforms. Such frequency components appear due to charge-separating (electrogenic) processes mediated by epithelial plasma membrane proteins (both ionic channels and ionic cotransporters). In particular, the endothelial TEPD oscillations of the highest amplitude (1-2 Hz) were linked to the operation of so-called sodium bicarbonate cotransporters. However, no time localization of that activity could be obtained with the Fourier methodology utilized. For that reason we now characterize the TEPD using wavelet analysis with the aim to localize in time the variations in TEPD. We find that the mentioned high-amplitude oscillatory components of the TEPD appear cyclically during the several hours that an endothelial preparation survives in vitro. They have a period of 4.6 ± 0.4 s on average (n=4). The wavelet power value at the peak of such oscillations is 1.5 ± 0.1 mV2 Hz on average (n = 4), and is remarkably narrow in its distribution.
Fil: Cacace, Verónica Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Montalbetti, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Kusnier, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Gomez, M. P.. Comisión Nacional de Energía Atómica; Argentina. Universidad Tecnológica Nacional; Argentina
Fil: Fischbarg, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
description The corneal endothelium is a fluid-transporting epithelium. As other similar tissues, it displays an electrical potential of ∼1 mV (aqueous side negative) across the entire layer [transendothelial potential difference (TEPD)]. It appears that this electrical potential is mainly the result of the transport of anions across the cell layer (from stroma to aqueous). There is substantial evidence that the TEPD is related linearly to fluid transport; hence, under proper conditions, its measure could serve as a measure of fluid transport. Furthermore, the TEPD is not steady; instead, it displays a spectrum of frequency components (0-15 Hz) recognized recently using Fourier transforms. Such frequency components appear due to charge-separating (electrogenic) processes mediated by epithelial plasma membrane proteins (both ionic channels and ionic cotransporters). In particular, the endothelial TEPD oscillations of the highest amplitude (1-2 Hz) were linked to the operation of so-called sodium bicarbonate cotransporters. However, no time localization of that activity could be obtained with the Fourier methodology utilized. For that reason we now characterize the TEPD using wavelet analysis with the aim to localize in time the variations in TEPD. We find that the mentioned high-amplitude oscillatory components of the TEPD appear cyclically during the several hours that an endothelial preparation survives in vitro. They have a period of 4.6 ± 0.4 s on average (n=4). The wavelet power value at the peak of such oscillations is 1.5 ± 0.1 mV2 Hz on average (n = 4), and is remarkably narrow in its distribution.
publishDate 2011
dc.date.none.fl_str_mv 2011-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67464
Cacace, Verónica Inés; Montalbetti, Nicolas; Kusnier, Carlos Federico; Gomez, M. P.; Fischbarg, Jorge; Wavelet analysis of corneal endothelial electrical potential difference reveals cyclic operation of the secretory mechanism; American Physical Society; Physical Review E: Statistical, Nonlinear and Soft Matter Physics; 84; 3; 9-2011; 1-5; 032902
1539-3755
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67464
identifier_str_mv Cacace, Verónica Inés; Montalbetti, Nicolas; Kusnier, Carlos Federico; Gomez, M. P.; Fischbarg, Jorge; Wavelet analysis of corneal endothelial electrical potential difference reveals cyclic operation of the secretory mechanism; American Physical Society; Physical Review E: Statistical, Nonlinear and Soft Matter Physics; 84; 3; 9-2011; 1-5; 032902
1539-3755
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1103/PhysRevE.84.032902
info:eu-repo/semantics/altIdentifier/url/https://journals.aps.org/pre/abstract/10.1103/PhysRevE.84.032902
info:eu-repo/semantics/altIdentifier/url/https://arxiv.org/abs/1106.3053
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Physical Society
publisher.none.fl_str_mv American Physical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269140231913472
score 13.13397

Publicaciones similares