Advancing gastrointestinal nematode treatment through phytochemicals administration to lambs

Autores
Miró, María Victoria; Lloberas, Maria Mercedes; Virkel, Guillermo Leon; Lifschitz, Adrian Luis
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
In the last 50 years, few antiparasitic drugs with new mechanisms of action have been introduced; leading to a global increase of drug resistance. Therefore, the search for alternative pharmacological tools is a priority in ruminant production systems. While numerous phytochemicals demonstrated efficacy against parasites in vitro, the transition to in vivo characterization poses a growing challenge. The drug concentration attained in the target parasites and, the resulting pharmacological effect are directly influenced by the administration route and pharmaceutical formulation. The aim of the current work was to analyze the pharmacokinetic-pharmacodynamic relationship of the combined administration of carvone (CNE) and ivermectin (IVM) to lambs. Three trials were conducted to evaluate the pharmacological interaction between CNE and IVM in lambs infected with nematodes. Drug concentrations were measured in plasma, target tissues and H. contortus by HPLC with fluorescent (IVM) and ultraviolet (CNE) detection. The decrease in fecal egg count was used as an indicator for estimating the efficacy of both compounds. CNE significantly enhanced the plasma bioavailability of IVM. CNE showed a moderate anthelmintic effect, which was greater on the susceptible isolate of H. contortus. After the combination of CNE and IVM as an oral emulsion, both compounds were quantified in H. contortus recovered from infected lambs. Although the coadministration of CNE and IVM in lambs demonstrated a moderate in vivo anthelmintic effect and enhanced systemic availability of IVM, the concentrations achieved in both target tissues and parasites remained notably lower compared to those documented to induce anthelmintic effects in the in vitro assays. Consequently, these levels were insufficient to achieve the desired optimal efficacy. Innovative pharmaceutical formulations are required to establish phytochemicals as a useful pharmacological tool for controlling nematodes in ruminants.
Fil: Miró, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Lloberas, Maria Mercedes. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Virkel, Guillermo Leon. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Materia
Anthelmintics
Carvone
Ivermectin
Lambs
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/221503
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Advancing gastrointestinal nematode treatment through phytochemicals administration to lambsMiró, María VictoriaLloberas, Maria MercedesVirkel, Guillermo LeonLifschitz, Adrian LuisAnthelminticsCarvoneIvermectinLambshttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4In the last 50 years, few antiparasitic drugs with new mechanisms of action have been introduced; leading to a global increase of drug resistance. Therefore, the search for alternative pharmacological tools is a priority in ruminant production systems. While numerous phytochemicals demonstrated efficacy against parasites in vitro, the transition to in vivo characterization poses a growing challenge. The drug concentration attained in the target parasites and, the resulting pharmacological effect are directly influenced by the administration route and pharmaceutical formulation. The aim of the current work was to analyze the pharmacokinetic-pharmacodynamic relationship of the combined administration of carvone (CNE) and ivermectin (IVM) to lambs. Three trials were conducted to evaluate the pharmacological interaction between CNE and IVM in lambs infected with nematodes. Drug concentrations were measured in plasma, target tissues and H. contortus by HPLC with fluorescent (IVM) and ultraviolet (CNE) detection. The decrease in fecal egg count was used as an indicator for estimating the efficacy of both compounds. CNE significantly enhanced the plasma bioavailability of IVM. CNE showed a moderate anthelmintic effect, which was greater on the susceptible isolate of H. contortus. After the combination of CNE and IVM as an oral emulsion, both compounds were quantified in H. contortus recovered from infected lambs. Although the coadministration of CNE and IVM in lambs demonstrated a moderate in vivo anthelmintic effect and enhanced systemic availability of IVM, the concentrations achieved in both target tissues and parasites remained notably lower compared to those documented to induce anthelmintic effects in the in vitro assays. Consequently, these levels were insufficient to achieve the desired optimal efficacy. Innovative pharmaceutical formulations are required to establish phytochemicals as a useful pharmacological tool for controlling nematodes in ruminants.Fil: Miró, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Lloberas, Maria Mercedes. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Virkel, Guillermo Leon. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaLXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de LaboratorioMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de BiologíaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioFundación Revista Medicina2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/221503Advancing gastrointestinal nematode treatment through phytochemicals administration to lambs; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 1-40025-7680CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2020/volumen-83-ano-2023-s5/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:12Zoai:ri.conicet.gov.ar:11336/221503instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:13.172CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Advancing gastrointestinal nematode treatment through phytochemicals administration to lambs
title Advancing gastrointestinal nematode treatment through phytochemicals administration to lambs
spellingShingle Advancing gastrointestinal nematode treatment through phytochemicals administration to lambs
Miró, María Victoria
Anthelmintics
Carvone
Ivermectin
Lambs
title_short Advancing gastrointestinal nematode treatment through phytochemicals administration to lambs
title_full Advancing gastrointestinal nematode treatment through phytochemicals administration to lambs
title_fullStr Advancing gastrointestinal nematode treatment through phytochemicals administration to lambs
title_full_unstemmed Advancing gastrointestinal nematode treatment through phytochemicals administration to lambs
title_sort Advancing gastrointestinal nematode treatment through phytochemicals administration to lambs
dc.creator.none.fl_str_mv Miró, María Victoria
Lloberas, Maria Mercedes
Virkel, Guillermo Leon
Lifschitz, Adrian Luis
author Miró, María Victoria
author_facet Miró, María Victoria
Lloberas, Maria Mercedes
Virkel, Guillermo Leon
Lifschitz, Adrian Luis
author_role author
author2 Lloberas, Maria Mercedes
Virkel, Guillermo Leon
Lifschitz, Adrian Luis
author2_role author
author
author
dc.subject.none.fl_str_mv Anthelmintics
Carvone
Ivermectin
Lambs
topic Anthelmintics
Carvone
Ivermectin
Lambs
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv In the last 50 years, few antiparasitic drugs with new mechanisms of action have been introduced; leading to a global increase of drug resistance. Therefore, the search for alternative pharmacological tools is a priority in ruminant production systems. While numerous phytochemicals demonstrated efficacy against parasites in vitro, the transition to in vivo characterization poses a growing challenge. The drug concentration attained in the target parasites and, the resulting pharmacological effect are directly influenced by the administration route and pharmaceutical formulation. The aim of the current work was to analyze the pharmacokinetic-pharmacodynamic relationship of the combined administration of carvone (CNE) and ivermectin (IVM) to lambs. Three trials were conducted to evaluate the pharmacological interaction between CNE and IVM in lambs infected with nematodes. Drug concentrations were measured in plasma, target tissues and H. contortus by HPLC with fluorescent (IVM) and ultraviolet (CNE) detection. The decrease in fecal egg count was used as an indicator for estimating the efficacy of both compounds. CNE significantly enhanced the plasma bioavailability of IVM. CNE showed a moderate anthelmintic effect, which was greater on the susceptible isolate of H. contortus. After the combination of CNE and IVM as an oral emulsion, both compounds were quantified in H. contortus recovered from infected lambs. Although the coadministration of CNE and IVM in lambs demonstrated a moderate in vivo anthelmintic effect and enhanced systemic availability of IVM, the concentrations achieved in both target tissues and parasites remained notably lower compared to those documented to induce anthelmintic effects in the in vitro assays. Consequently, these levels were insufficient to achieve the desired optimal efficacy. Innovative pharmaceutical formulations are required to establish phytochemicals as a useful pharmacological tool for controlling nematodes in ruminants.
Fil: Miró, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Lloberas, Maria Mercedes. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Virkel, Guillermo Leon. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
description In the last 50 years, few antiparasitic drugs with new mechanisms of action have been introduced; leading to a global increase of drug resistance. Therefore, the search for alternative pharmacological tools is a priority in ruminant production systems. While numerous phytochemicals demonstrated efficacy against parasites in vitro, the transition to in vivo characterization poses a growing challenge. The drug concentration attained in the target parasites and, the resulting pharmacological effect are directly influenced by the administration route and pharmaceutical formulation. The aim of the current work was to analyze the pharmacokinetic-pharmacodynamic relationship of the combined administration of carvone (CNE) and ivermectin (IVM) to lambs. Three trials were conducted to evaluate the pharmacological interaction between CNE and IVM in lambs infected with nematodes. Drug concentrations were measured in plasma, target tissues and H. contortus by HPLC with fluorescent (IVM) and ultraviolet (CNE) detection. The decrease in fecal egg count was used as an indicator for estimating the efficacy of both compounds. CNE significantly enhanced the plasma bioavailability of IVM. CNE showed a moderate anthelmintic effect, which was greater on the susceptible isolate of H. contortus. After the combination of CNE and IVM as an oral emulsion, both compounds were quantified in H. contortus recovered from infected lambs. Although the coadministration of CNE and IVM in lambs demonstrated a moderate in vivo anthelmintic effect and enhanced systemic availability of IVM, the concentrations achieved in both target tissues and parasites remained notably lower compared to those documented to induce anthelmintic effects in the in vitro assays. Consequently, these levels were insufficient to achieve the desired optimal efficacy. Innovative pharmaceutical formulations are required to establish phytochemicals as a useful pharmacological tool for controlling nematodes in ruminants.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/221503
Advancing gastrointestinal nematode treatment through phytochemicals administration to lambs; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 1-4
0025-7680
CONICET Digital
CONICET
url http://hdl.handle.net/11336/221503
identifier_str_mv Advancing gastrointestinal nematode treatment through phytochemicals administration to lambs; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 1-4
0025-7680
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2020/volumen-83-ano-2023-s5/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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