Electrochemical biointerfaces based on carbon nanotubes-mesoporous silica hybrid material: Bioelectrocatalysis of hemoglobin and biosensing applications

Autores
Eguílaz Rubio, Marcos; Villalonga, Reynaldo; Rivas, Gustavo Adolfo
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We are reporting a novel biosensing platform based on a hybrid nanomaterial that combines the advantages of Nafion-coated multiwalled carbon nanotubes (MWCNTs) and mesoporous silica MCM41 nanoparticles functionalized with hemoglobin (Hb). MWCNTs-MCM41-Hb hybrid bioconjugate was characterized by scanning electron microscopy (SEM), UV–vis spectroscopy and electrochemical techniques after deposition at glassy carbon electrodes (GCE). The combination of the high surface area, biocompatibility and protein loading capacity of MCM41 nanoparticles and the high surface area and catalytic properties of MWCNTs allowed the direct electron transfer (DET) between Hb and the electrode surface. The electron transfer rate constant (k) and the surface coverage of electroactive Hb (ΓHb) were 5.2 s−1 and 4.7 × 10−10 mol cm−2, respectively. The GCE modified with the nanostructured architecture (GCE/MWCNTs-MCM41-Hb) was successfully used as a third-generation biosensor for the highly sensitive and selective quantification of nitrite (NO2 -) and trichloroacetic acid (TCA) by taking advantage of the excellent biocatalytic activity of Hb and the efficient direct charge transfer of the heme group.
Fil: Eguílaz Rubio, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Villalonga, Reynaldo. Universidad Complutense de Madrid; España
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
CARBON NANOTUBES
HEMOGLOBIN
HYBRID MATERIALS
MESOPOROUS SILICA
NITRITE
TRICHLOROACETIC ACID
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/90788
Acceder
id CONICETDig_ef868356800069ad4cf69891aedaba42
oai_identifier_str oai:ri.conicet.gov.ar:11336/90788
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Electrochemical biointerfaces based on carbon nanotubes-mesoporous silica hybrid material: Bioelectrocatalysis of hemoglobin and biosensing applicationsEguílaz Rubio, MarcosVillalonga, ReynaldoRivas, Gustavo AdolfoCARBON NANOTUBESHEMOGLOBINHYBRID MATERIALSMESOPOROUS SILICANITRITETRICHLOROACETIC ACIDhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We are reporting a novel biosensing platform based on a hybrid nanomaterial that combines the advantages of Nafion-coated multiwalled carbon nanotubes (MWCNTs) and mesoporous silica MCM41 nanoparticles functionalized with hemoglobin (Hb). MWCNTs-MCM41-Hb hybrid bioconjugate was characterized by scanning electron microscopy (SEM), UV–vis spectroscopy and electrochemical techniques after deposition at glassy carbon electrodes (GCE). The combination of the high surface area, biocompatibility and protein loading capacity of MCM41 nanoparticles and the high surface area and catalytic properties of MWCNTs allowed the direct electron transfer (DET) between Hb and the electrode surface. The electron transfer rate constant (k) and the surface coverage of electroactive Hb (ΓHb) were 5.2 s−1 and 4.7 × 10−10 mol cm−2, respectively. The GCE modified with the nanostructured architecture (GCE/MWCNTs-MCM41-Hb) was successfully used as a third-generation biosensor for the highly sensitive and selective quantification of nitrite (NO2 -) and trichloroacetic acid (TCA) by taking advantage of the excellent biocatalytic activity of Hb and the efficient direct charge transfer of the heme group.Fil: Eguílaz Rubio, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Villalonga, Reynaldo. Universidad Complutense de Madrid; EspañaFil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaElsevier Advanced Technology2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/90788Eguílaz Rubio, Marcos; Villalonga, Reynaldo; Rivas, Gustavo Adolfo; Electrochemical biointerfaces based on carbon nanotubes-mesoporous silica hybrid material: Bioelectrocatalysis of hemoglobin and biosensing applications; Elsevier Advanced Technology; Biosensors & Bioelectronics; 111; 7-2018; 144-1510956-5663CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0956566318302586info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bios.2018.04.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:22:06Zoai:ri.conicet.gov.ar:11336/90788instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:22:06.343CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Electrochemical biointerfaces based on carbon nanotubes-mesoporous silica hybrid material: Bioelectrocatalysis of hemoglobin and biosensing applications
title Electrochemical biointerfaces based on carbon nanotubes-mesoporous silica hybrid material: Bioelectrocatalysis of hemoglobin and biosensing applications
spellingShingle Electrochemical biointerfaces based on carbon nanotubes-mesoporous silica hybrid material: Bioelectrocatalysis of hemoglobin and biosensing applications
Eguílaz Rubio, Marcos
CARBON NANOTUBES
HEMOGLOBIN
HYBRID MATERIALS
MESOPOROUS SILICA
NITRITE
TRICHLOROACETIC ACID
title_short Electrochemical biointerfaces based on carbon nanotubes-mesoporous silica hybrid material: Bioelectrocatalysis of hemoglobin and biosensing applications
title_full Electrochemical biointerfaces based on carbon nanotubes-mesoporous silica hybrid material: Bioelectrocatalysis of hemoglobin and biosensing applications
title_fullStr Electrochemical biointerfaces based on carbon nanotubes-mesoporous silica hybrid material: Bioelectrocatalysis of hemoglobin and biosensing applications
title_full_unstemmed Electrochemical biointerfaces based on carbon nanotubes-mesoporous silica hybrid material: Bioelectrocatalysis of hemoglobin and biosensing applications
title_sort Electrochemical biointerfaces based on carbon nanotubes-mesoporous silica hybrid material: Bioelectrocatalysis of hemoglobin and biosensing applications
dc.creator.none.fl_str_mv Eguílaz Rubio, Marcos
Villalonga, Reynaldo
Rivas, Gustavo Adolfo
author Eguílaz Rubio, Marcos
author_facet Eguílaz Rubio, Marcos
Villalonga, Reynaldo
Rivas, Gustavo Adolfo
author_role author
author2 Villalonga, Reynaldo
Rivas, Gustavo Adolfo
author2_role author
author
dc.subject.none.fl_str_mv CARBON NANOTUBES
HEMOGLOBIN
HYBRID MATERIALS
MESOPOROUS SILICA
NITRITE
TRICHLOROACETIC ACID
topic CARBON NANOTUBES
HEMOGLOBIN
HYBRID MATERIALS
MESOPOROUS SILICA
NITRITE
TRICHLOROACETIC ACID
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We are reporting a novel biosensing platform based on a hybrid nanomaterial that combines the advantages of Nafion-coated multiwalled carbon nanotubes (MWCNTs) and mesoporous silica MCM41 nanoparticles functionalized with hemoglobin (Hb). MWCNTs-MCM41-Hb hybrid bioconjugate was characterized by scanning electron microscopy (SEM), UV–vis spectroscopy and electrochemical techniques after deposition at glassy carbon electrodes (GCE). The combination of the high surface area, biocompatibility and protein loading capacity of MCM41 nanoparticles and the high surface area and catalytic properties of MWCNTs allowed the direct electron transfer (DET) between Hb and the electrode surface. The electron transfer rate constant (k) and the surface coverage of electroactive Hb (ΓHb) were 5.2 s−1 and 4.7 × 10−10 mol cm−2, respectively. The GCE modified with the nanostructured architecture (GCE/MWCNTs-MCM41-Hb) was successfully used as a third-generation biosensor for the highly sensitive and selective quantification of nitrite (NO2 -) and trichloroacetic acid (TCA) by taking advantage of the excellent biocatalytic activity of Hb and the efficient direct charge transfer of the heme group.
Fil: Eguílaz Rubio, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Villalonga, Reynaldo. Universidad Complutense de Madrid; España
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description We are reporting a novel biosensing platform based on a hybrid nanomaterial that combines the advantages of Nafion-coated multiwalled carbon nanotubes (MWCNTs) and mesoporous silica MCM41 nanoparticles functionalized with hemoglobin (Hb). MWCNTs-MCM41-Hb hybrid bioconjugate was characterized by scanning electron microscopy (SEM), UV–vis spectroscopy and electrochemical techniques after deposition at glassy carbon electrodes (GCE). The combination of the high surface area, biocompatibility and protein loading capacity of MCM41 nanoparticles and the high surface area and catalytic properties of MWCNTs allowed the direct electron transfer (DET) between Hb and the electrode surface. The electron transfer rate constant (k) and the surface coverage of electroactive Hb (ΓHb) were 5.2 s−1 and 4.7 × 10−10 mol cm−2, respectively. The GCE modified with the nanostructured architecture (GCE/MWCNTs-MCM41-Hb) was successfully used as a third-generation biosensor for the highly sensitive and selective quantification of nitrite (NO2 -) and trichloroacetic acid (TCA) by taking advantage of the excellent biocatalytic activity of Hb and the efficient direct charge transfer of the heme group.
publishDate 2018
dc.date.none.fl_str_mv 2018-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/90788
Eguílaz Rubio, Marcos; Villalonga, Reynaldo; Rivas, Gustavo Adolfo; Electrochemical biointerfaces based on carbon nanotubes-mesoporous silica hybrid material: Bioelectrocatalysis of hemoglobin and biosensing applications; Elsevier Advanced Technology; Biosensors & Bioelectronics; 111; 7-2018; 144-151
0956-5663
CONICET Digital
CONICET
url http://hdl.handle.net/11336/90788
identifier_str_mv Eguílaz Rubio, Marcos; Villalonga, Reynaldo; Rivas, Gustavo Adolfo; Electrochemical biointerfaces based on carbon nanotubes-mesoporous silica hybrid material: Bioelectrocatalysis of hemoglobin and biosensing applications; Elsevier Advanced Technology; Biosensors & Bioelectronics; 111; 7-2018; 144-151
0956-5663
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0956566318302586
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bios.2018.04.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Advanced Technology
publisher.none.fl_str_mv Elsevier Advanced Technology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781733903532032
score 12.982451

Publicaciones similares